Peter Walter

Summary

Affiliation: University of California
Country: USA

Publications

  1. doi request reprint Science as a Way of Knowing: From Protein Machines to Evidence-Based Decisions
    Peter Walter
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94143, USA Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA Electronic address
    Cell 167:16-9. 2016
  2. doi request reprint The unfolded protein response: from stress pathway to homeostatic regulation
    Peter Walter
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    Science 334:1081-6. 2011
  3. pmc The unfolded protein response signals through high-order assembly of Ire1
    Alexei V Korennykh
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Nature 457:687-93. 2009
  4. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
  5. pmc Mechanism of association and reciprocal activation of two GTPases
    Shu ou Shan
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:e320. 2004
  6. pmc BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response
    David Pincus
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS Biol 8:e1000415. 2010
  7. pmc Gene recruitment of the activated INO1 locus to the nuclear membrane
    Jason H Brickner
    Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA
    PLoS Biol 2:e342. 2004
  8. pmc Seg1 controls eisosome assembly and shape
    Karen E Moreira
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 198:405-20. 2012
  9. pmc X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates
    Christopher L Reyes
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e607. 2007
  10. pmc SRP RNA controls a conformational switch regulating the SRP-SRP receptor interaction
    Saskia B Neher
    Howard Hughes Medical Institute, University of California at San Francisco, 600 16th Street, San Francisco, California 94158, USA
    Nat Struct Mol Biol 15:916-23. 2008

Detail Information

Publications71

  1. doi request reprint Science as a Way of Knowing: From Protein Machines to Evidence-Based Decisions
    Peter Walter
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94143, USA Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143, USA Electronic address
    Cell 167:16-9. 2016
    ....
  2. doi request reprint The unfolded protein response: from stress pathway to homeostatic regulation
    Peter Walter
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    Science 334:1081-6. 2011
    ..Recent advances shed light on mechanistic complexities and on the role of the UPR in numerous diseases...
  3. pmc The unfolded protein response signals through high-order assembly of Ire1
    Alexei V Korennykh
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Nature 457:687-93. 2009
    ..Activation of Ire1 through oligomerization expands the mechanistic repertoire of kinase-based signalling receptors...
  4. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
    ..The changes in the ER lumen in turn position Ire1 kinase domains in the cytoplasm optimally for autophosphorylation to initiate the UPR...
  5. pmc Mechanism of association and reciprocal activation of two GTPases
    Shu ou Shan
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:e320. 2004
    ..Each stage provides a potential control point in the targeting reaction at which regulation by additional components can be exerted, thus ensuring the binding and release of cargo at the appropriate time...
  6. pmc BiP binding to the ER-stress sensor Ire1 tunes the homeostatic behavior of the unfolded protein response
    David Pincus
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS Biol 8:e1000415. 2010
    ..By contrast, BiP provides a buffer for inactive Ire1 molecules that ensures an appropriate response to restore protein folding homeostasis to the ER by modulating the sensitivity and dynamics of Ire1 activity...
  7. pmc Gene recruitment of the activated INO1 locus to the nuclear membrane
    Jason H Brickner
    Howard Hughes Medical Institute and the Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA
    PLoS Biol 2:e342. 2004
    ..Gene recruitment to the nuclear periphery, therefore, is a dynamic process and appears to play an important regulatory role...
  8. pmc Seg1 controls eisosome assembly and shape
    Karen E Moreira
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 198:405-20. 2012
    ..The function of Seg1 in the stepwise biogenesis of eisosomes reveals striking architectural similarities between eisosomes in yeast and caveolae in mammals...
  9. pmc X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates
    Christopher L Reyes
    Graduate Group in Biophysics, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e607. 2007
    ..We propose that these structural changes represent discrete conformational states assumed by FtsY during targeting complex formation and dissociation...
  10. pmc SRP RNA controls a conformational switch regulating the SRP-SRP receptor interaction
    Saskia B Neher
    Howard Hughes Medical Institute, University of California at San Francisco, 600 16th Street, San Francisco, California 94158, USA
    Nat Struct Mol Biol 15:916-23. 2008
    ..These results demonstrate that the N-terminal helices of SRP and SR are autoinhibitory for complex formation in the absence of SRP RNA, suggesting a mechanism for RNA-mediated coordination of the SRP-SR interaction...
  11. pmc Pkh-kinases control eisosome assembly and organization
    Tobias C Walther
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, USA
    EMBO J 26:4946-55. 2007
    ..Taken together with our previous results that link eisosomes to endocytosis, these observations suggest that Pkh-kinase signaling relayed to Pil1 may help regulate endocytic events to modulate the organization of the plasma membrane...
  12. pmc Signal sequences activate the catalytic switch of SRP RNA
    Niels Bradshaw
    Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA
    Science 323:127-30. 2009
    ..Thus, SRP RNA is a molecular switch that renders the SRP-SR GTPase engine responsive to signal peptide recruitment, coupling GTP hydrolysis to productive protein targeting...
  13. pmc Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS ONE 3:e3619. 2008
    ..Based on previous structures of two sub-complexes, we propose a model of the core of archeal and eukaryotic SRP*SR targeting complexes...
  14. pmc The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting
    Niels Bradshaw
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, CA 94158, USA
    Mol Biol Cell 18:2728-34. 2007
    ..In this way, the SRP RNA may coordinate the interaction of the SRP and the SR with ribosome recruitment and transfer to the translocon, explaining why the SRP RNA is an indispensable component of the protein targeting machinery...
  15. pmc Homeostatic adaptation to endoplasmic reticulum stress depends on Ire1 kinase activity
    Claudia Rubio
    Howard Hughes Medical Institute, San Francisco, CA, USA
    J Cell Biol 193:171-84. 2011
    ..Thus, Ire1-catalyzed phosphoryl-transfer aids disassembly of Ire1 signaling complexes and is a critical component of the UPR homeostatic feedback loop...
  16. pmc Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
    Martin C Jonikas
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143, USA
    Science 323:1693-7. 2009
    ..The use of a quantitative reporter in a comprehensive screen followed by systematic analysis of genetic dependencies should be broadly applicable to functional dissection of complex cellular processes from yeast to human...
  17. pmc Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry
    Feixia Chu
    Mass Spectrometry Facility, University of California, San Francisco 94143 0046, USA
    Proc Natl Acad Sci U S A 101:16454-9. 2004
    ..FtsY interface in the complex...
  18. pmc Gcn4p and novel upstream activating sequences regulate targets of the unfolded protein response
    Christopher K Patil
    Howard Hughes Medical Institute, Chevy Chase, Maryland, USA
    PLoS Biol 2:E246. 2004
    ..The discovery of a role for Gcn4p in the yeast UPR reveals an additional level of complexity and demonstrates a surprising conservation of the signaling circuit between yeast and metazoan cells...
  19. pmc Cofactor-mediated conformational control in the bifunctional kinase/RNase Ire1
    Alexei V Korennykh
    Howard Hughes Medical Institute, University of California, San Francisco, 600 16th Street, Room S272, Box 0724, San Francisco, CA 94158, USA
    BMC Biol 9:48. 2011
    ..In this study, we investigated how the binding of cofactors to the kinase domain of Ire1 modulates its RNase activity...
  20. pmc Mammalian endoplasmic reticulum stress sensor IRE1 signals by dynamic clustering
    Han Li
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:16113-8. 2010
    ..Thus IRE1 activity is governed by a timer that may be important in switching the UPR from the initially cytoprotective phase to the apoptotic mode...
  21. pmc Induced nucleotide specificity in a GTPase
    Shu ou Shan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Proc Natl Acad Sci U S A 100:4480-5. 2003
    ..We propose that this conformational change leads to more accurate positioning of the nucleotide and thus could contribute to activation of FtsY's GTPase activity by a novel mechanism...
  22. pmc Genome-scale approaches for discovering novel nonconventional splicing substrates of the Ire1 nuclease
    Maho Niwa
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
    Genome Biol 6:R3. 2005
    ..Because only the spliced mRNA is translated, splicing is the key regulatory step of the UPR...
  23. pmc Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses
    Polly M Fordyce
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 109:E3084-93. 2012
    ..Our results also suggest that even structurally simple transcription factors can recognize multiple divergent target sites of very different lengths, potentially enriching their downstream target repertoire...
  24. pmc Messenger RNA targeting to endoplasmic reticulum stress signalling sites
    TOMAS ARAGON
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158 2517, USA
    Nature 457:736-40. 2009
    ..Translational repression afforded by the intron fulfils this requirement for HAC1 mRNA. Recruitment of mRNA to signalling centres provides a new paradigm for the control of eukaryotic gene expression...
  25. pmc Unfolded proteins are Ire1-activating ligands that directly induce the unfolded protein response
    Brooke M Gardner
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    Science 333:1891-4. 2011
    ..Peptide binding caused Ire1 cLD oligomerization in vitro, suggesting that direct binding to unfolded proteins activates the UPR...
  26. pmc Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, USA
    PLoS ONE 3:e3528. 2008
    ....
  27. pmc Pil1 controls eisosome biogenesis
    Karen E Moreira
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 20:809-18. 2009
    ..Pil1 expression is regulated by the cell cycle, which synchronizes eisosome formation with plasma membrane growth. Our results establish a first framework of the molecular principles that define eisosome assembly and distribution...
  28. ncbi request reprint Targeting proteins to membranes: structure of the signal recognition particle
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
    Curr Opin Struct Biol 15:213-20. 2005
    ....
  29. ncbi request reprint Molecular crosstalk between the nucleotide specificity determinant of the SRP GTPase and the SRP receptor
    Shu ou Shan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 2200, USA
    Biochemistry 44:6214-22. 2005
    ..FtsY complex...
  30. pmc Structural and functional basis for RNA cleavage by Ire1
    Alexei V Korennykh
    Howard Hughes Medical Institute, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94158, USA
    BMC Biol 9:47. 2011
    ..Here, we experimentally define the active site of Ire1 RNase and quantitatively evaluate the contribution of the key active site residues to catalysis...
  31. pmc The yeast cell fusion protein Prm1p requires covalent dimerization to promote membrane fusion
    Alex Engel
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 5:e10593. 2010
    ..The importance of intermolecular disulfide bonding informs models regarding the mechanism of Prm1-mediated cell-cell fusion...
  32. pmc Divergent effects of PERK and IRE1 signaling on cell viability
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e4170. 2009
    ..These results demonstrate that extended PERK and IRE1 signaling have opposite effects on cell viability. Differential activation of PERK and IRE1 may determine life or death decisions after ER protein misfolding...
  33. pmc Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating
    Pablo S Aguilar
    Departments of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 107:4170-5. 2010
    ..Thus, a two-atom change in the aliphatic moiety of ergosterol is sufficient to obstruct cell shape remodeling and cell fusion...
  34. pmc IRE1 signaling affects cell fate during the unfolded protein response
    Jonathan H Lin
    Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 318:944-9. 2007
    ..Key findings from our studies in cell culture were recapitulated in photoreceptors expressing mutant rhodopsin in animal models of retinitis pigmentosa...
  35. pmc Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response
    Sebastian Schuck
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 187:525-36. 2009
    ..Thus, increasing ER size through membrane synthesis is an integral yet distinct part of the cellular program to overcome ER stress...
  36. pmc A CHOP-regulated microRNA controls rhodopsin expression
    Shannon Behrman
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    J Cell Biol 192:919-27. 2011
    ....
  37. ncbi request reprint Eisosomes mark static sites of endocytosis
    Tobias C Walther
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, 600 16th Street, San Francisco, California 94143 2200, USA
    Nature 439:998-1003. 2006
    ..Loss of Pil1 leads to clustering of eisosome remnants and redirects endocytosis and endocytic effector proteins to these clusters...
  38. pmc Lipase maturation factor LMF1, membrane topology and interaction with lipase proteins in the endoplasmic reticulum
    Mark H Doolittle
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    J Biol Chem 284:33623-33. 2009
    ..We discuss the implication of our results with regard to lipase maturation and DUF1222 domain structure...
  39. doi request reprint Structure-Activity Studies of Bis-O-Arylglycolamides: Inhibitors of the Integrated Stress Response
    Brian R Hearn
    Department of Pharmaceutical Chemistry and, Small Molecule Discovery Center, University of California, San Francisco, CA, 94158, USA
    ChemMedChem 11:870-80. 2016
    ..The SAR studies described herein support the proposed pharmacology of ISRIB analogues as binding across a symmetrical protein-protein interface formed between protein subunits of the dimeric eIF2B heteropentamer. ..
  40. pmc Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response
    Sebastián Bernales
    Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, United States of America
    PLoS Biol 4:e423. 2006
    ..Selective ER sequestration may help cells to maintain a new steady-state level of ER abundance even in the face of continuously accumulating unfolded proteins...
  41. ncbi request reprint Intracellular signaling by the unfolded protein response
    Sebastián Bernales
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
    Annu Rev Cell Dev Biol 22:487-508. 2006
    ....
  42. pmc ERMES-mediated ER-mitochondria contacts: molecular hubs for the regulation of mitochondrial biology
    Benoît Kornmann
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California at San Francisco, CA 94158, USA
    J Cell Sci 123:1389-93. 2010
    ..Finally, we discuss how cells might use ER-mitochondria communication to fine-tune these processes according to their metabolic needs...
  43. pmc The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating
    Maxwell G Heiman
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 176:209-22. 2007
    ..Kex2 and Kex1 may promote cell fusion by proteolytically processing substrates that act in parallel to Prm1 as an alternative fusion machine, as cell wall components, or both...
  44. pmc IRE1-independent gain control of the unfolded protein response
    Jess H Leber
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:E235. 2004
    ..We show that by adding an additional signaling element to the basic UPR circuit, a simple switch is transformed into a complex response...
  45. pmc Endoplasmic reticulum stress sensing in the unfolded protein response
    Brooke M Gardner
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94158, USA
    Cold Spring Harb Perspect Biol 5:a013169. 2013
    ..BiP dissociation from Ire1 regulates this oligomeric equilibrium, ultimately modulating Ire1's sensitivity and duration of activation. The mechanistic principles of ER stress sensing are the focus of this review...
  46. pmc An ER-mitochondria tethering complex revealed by a synthetic biology screen
    Benoît Kornmann
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Science 325:477-81. 2009
    ..The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange...
  47. ncbi request reprint Co-translational protein targeting by the signal recognition particle
    Shu ou Shan
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    FEBS Lett 579:921-6. 2005
    ..SR complex. Each stage provides a potential control point in the targeting reaction at which regulation by additional components can be exerted, thus ensuring the binding and release of cargo at the appropriate time...
  48. pmc The conserved GTPase Gem1 regulates endoplasmic reticulum-mitochondria connections
    Benoît Kornmann
    Department of Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 108:14151-6. 2011
    ..Furthermore, we provide evidence that the metazoan Gem1 ortholog Miro-1 localizes to sites of ER-mitochondrial contact, suggesting that some of the features ascribed to Gem1 may be evolutionarily conserved...
  49. ncbi request reprint ER-phagy: selective autophagy of the endoplasmic reticulum
    Sebastián Bernales
    Howard Hughes Medical Institute, Department of Biochemistry, School of Medicine, University of California San Francisco, San Francisco, California 94158 2517, USA
    Autophagy 3:285-7. 2007
    ..ER-phagy could remove damaged or redundant parts of the ER and thus represent an important degradative functionality of the UPR that helps to afford homeostatic control...
  50. pmc Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic
    Aaron S Mendez
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United States
    elife 4:. 2015
    ..The mechanism by which partial occupancy with an inhibitor can activate kinases may be wide-spread and carries major implications for design and therapeutic application of kinase inhibitors. ..
  51. pmc Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli
    Bentley Lim
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California, United States of America
    PLoS Biol 11:e1001735. 2013
    ..Our results show that σ(32) must be membrane-associated to be properly regulated in response to the protein folding status in the cell, explaining how the HSR integrates information from both the cytoplasm and bacterial cell membrane. ..
  52. pmc The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating
    Pablo S Aguilar
    Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 18:547-56. 2007
    ..Thus, our results suggest that Prm1 and Fig1 have a role in enhancing membrane fusion and maintaining its fidelity. Their absence results in frequent mating pair lysis, which is counteracted by Ca(2+)-dependent membrane repair...
  53. pmc A first line of defense against ER stress
    David Pincus
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 198:277-9. 2012
    ..In this issue, Chambers et al. (2012. J. Cell Biol. doi:10.1083/jcb.201202005) report that ADP ribosylation of BiP provides a reversible switch that fine tunes BiP activity according to need...
  54. pmc Membrane lysis during biological membrane fusion: collateral damage by misregulated fusion machines
    Alex Engel
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 183:181-6. 2008
    ..The convergence of these findings showing membrane integrity loss during biological membrane fusion suggests new mechanistic models for membrane fusion and the role of membrane fusion complexes...
  55. ncbi request reprint Substrate twinning activates the signal recognition particle and its receptor
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California at San Francisco, California 94143 2240, USA
    Nature 427:215-21. 2004
    ..This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery...
  56. pmc Walking along the serendipitous path of discovery
    Peter Walter
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143 2200, USA
    Mol Biol Cell 21:15-7. 2010
    ..A key regulatory switch in the UPR, for example, is a cytoplasmic, nonconventional mRNA spicing reaction, initiated by a bifunctional transmembrane kinase/endoribonuclease...
  57. pmc Endoplasmic reticulum stress in disease pathogenesis
    Jonathan H Lin
    Department of Biochemistry, University of California, San Francisco, CA 94143, USA
    Annu Rev Pathol 3:399-425. 2008
    ..This review provides background information on the unfolded protein response and discusses a selection of diseases whose pathogenesis involves ER stress...
  58. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  59. pmc The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis
    Philipp Kimmig
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States
    elife 1:e00048. 2012
    ..Thus, S. pombe uses a universally conserved stress-sensing machinery in novel ways to maintain homeostasis in the ER.DOI:http://dx.doi.org/10.7554/eLife.00048.001...
  60. pmc A conformational RNA zipper promotes intron ejection during non-conventional XBP1 mRNA splicing
    Jirka Peschek
    Department of Biochemistry and Biophysics and Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA, USA
    EMBO Rep 16:1688-98. 2015
    ....
  61. pmc Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6α branch
    Ciara M Gallagher
    Department of Biochemistry and Biophysics, Howard Hughes MedicaI Institute, University of California, San Francisco, United States
    elife 5:. 2016
    ..The discovery of Ceapins now enables pharmacological modulation all three UPR branches either singly or in combination. ..
  62. pmc ER-phagy mediates selective degradation of endoplasmic reticulum independently of the core autophagy machinery
    Sebastian Schuck
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA
    J Cell Sci 127:4078-88. 2014
    ..Finally, we provide evidence that ER-phagy degrades excess ER membrane, suggesting that it contributes to cell homeostasis by controlling organelle size. ..
  63. pmc Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response
    Carmela Sidrauski
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States
    elife 4:e07314. 2015
    ..By modulating eIF2B function, ISRIB promises to be an invaluable tool in proof-of-principle studies aiming to ameliorate cognitive defects resulting from neurodegenerative diseases. ..
  64. pmc ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
    Andrew Murley
    Department of Molecular and Cellular Biology, University of California, Davis, Davis, United States
    elife 2:e00422. 2013
    ..Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI:http://dx.doi.org/10.7554/eLife.00422.001...
  65. pmc Pharmacological brake-release of mRNA translation enhances cognitive memory
    Carmela Sidrauski
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United States
    elife 2:e00498. 2013
    ..Thus, memory consolidation is inherently limited by the ISR, and ISRIB releases this brake. As such, ISRIB promises to contribute to our understanding and treatment of cognitive disorders. DOI:http://dx.doi.org/10.7554/eLife.00498.001...
  66. pmc Identification of yeast proteins necessary for cell-surface function of a potassium channel
    Friederike A Haass
    Department of Physiology, Neuroscience Graduate Program, and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 104:18079-84. 2007
    ..The seven yeast proteins identified in our screen likely impact Kir-channel functional expression at the level of vesicle budding from the ER and/or the local lipid environment at the plasma membrane...
  67. pmc Translation from the 5' untranslated region shapes the integrated stress response
    Shelley R Starck
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Science 351:aad3867. 2016
    ....
  68. pmc Ceapins inhibit ATF6α signaling by selectively preventing transport of ATF6α to the Golgi apparatus during ER stress
    Ciara M Gallagher
    Howard Hughes MedicaI Institute, University of California, San Francisco, San Francisco, United States
    elife 5:. 2016
    ..Washout of Ceapins resensitized ATF6α to ER stress. These results suggest that trafficking of ATF6α is regulated by its oligomeric state. ..
  69. pmc The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stress granule assembly
    Carmela Sidrauski
    Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francis, San Francisco, United States
    elife 4:. 2015
    ..Restoration of mRNA translation and modulation of SG dynamics may be an effective treatment of neurodegenerative diseases characterized by eIF2α phosphorylation, SG formation, and cognitive loss. ..
  70. ncbi request reprint Bypassing a kinase activity with an ATP-competitive drug
    Feroz R Papa
    Department of Medicine, University of California, San Francisco, CA 94143 2200, USA
    Science 302:1533-7. 2003
    ..Thus, rather than through phosphorylation per se, a conformational change in the kinase domain triggered by occupancy of the active site with a ligand leads to activation of all known downstream functions...