Affiliation: University of California
- Genetic parts to program bacteriaChristopher A Voigt
Biophysics and Chemistry and Chemical Biology, Department of Pharmaceutical Chemistry, University of California San Francisco, QB3 Box 2540, 1700 4th Street, San Francisco, CA 94158, USA
Curr Opin Biotechnol 17:548-57. 2006..On the basis of these developments, increasingly complex cellular machines are being constructed to build specialty chemicals, weave biomaterials, and to deliver therapeutics...
- Environmental signal integration by a modular AND gateJ Christopher Anderson
Department of Pharmaceutical Chemistry, QB3 California Institute for Quantitative Biological Research, The University of California San Francisco, San Francisco, CA 94158 2330, USA
Mol Syst Biol 3:133. 2007..A mathematical model of the transfer function is derived and parameterized using experimental data...
- Engineering the Salmonella type III secretion system to export spider silk monomersDaniel M Widmaier
Chemistry and Chemical Biology Graduate Program, University of California San Francisco, San Francisco, CA 94110, USA
Mol Syst Biol 5:309. 2009..8 mg l(-1) h(-1) are demonstrated with up to 14% of expressed protein secreted. This work introduces new parts to control protein secretion in Gram-negative bacteria, which will be broadly applicable to problems in biotechnology...
- Modular control of multiple pathways using engineered orthogonal T7 polymerasesKarsten Temme
UCB UCSF Joint Graduate Group in Bioengineering, MC2540, Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 1700 4th Street, San Francisco, CA 94158, USA
Nucleic Acids Res 40:8773-81. 2012....
- Programming cellular functionChristopher A Voigt
Department of Pharmaceutical Chemistry and California Institute for Quantitative Biomedical Research QB3, University of California, San Francisco, San Francisco, California 94143 2540, USA
Nat Chem Biol 1:304-7. 2005..A meeting was held in August 2005 to present progress in the field and to discuss topics in ethics, safety and security...
- The Bacillus subtilis sin operon: an evolvable network motifChristopher A Voigt
Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
Genetics 169:1187-202. 2005..The dynamical plasticity of the protein-antagonist operon motif suggests that it is an evolutionarily convergent design selected not only for particular immediate function but also for its evolvability...
- Engineering bacterial signals and sensorsHoward Salis
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94158, USA
Contrib Microbiol 16:194-225. 2009..We also provide specification sheets describing many two-component and quorum-sensing systems, focusing on the information that one needs to know in order to use them for engineering applications...
- Environmentally controlled invasion of cancer cells by engineered bacteriaJ Christopher Anderson
Howard Hughes Medical Institute, California Institute of Quantitative Biology Department of Bioengineering, University of California, 717 Potter Street, Room 257 Berkeley, CA 94720, USA
J Mol Biol 355:619-27. 2006..This approach could be used to engineer bacteria to sense the microenvironment of a tumor and respond by invading cancerous cells and releasing a cytotoxic agent...
- Induction and relaxation dynamics of the regulatory network controlling the type III secretion system encoded within Salmonella pathogenicity island 1Karsten Temme
UCSF UCB Joint Graduate Group in Bioengineering, San Francisco, CA, USA
J Mol Biol 377:47-61. 2008..This combination of theory and experiments provides a platform for predicting how genetic perturbations lead to changes in the global dynamics of a regulatory network...
- Synthesis of methyl halides from biomass using engineered microbesTravis S Bayer
Department of Pharmaceutical Chemistry, University of California, San Francisco, MC 2540, Room 408C, 1700 4th Street, San Francisco, California 94158 2330, USA
J Am Chem Soc 131:6508-15. 2009..These results demonstrate the potential of producing methyl halides from non-food agricultural resources...
- Synthetic biology: engineering Escherichia coli to see lightAnselm Levskaya
Biophysics Program, University of California, San Francisco, California 94143, USA
Nature 438:441-2. 2005..This spatial control of bacterial gene expression could be used to 'print' complex biological materials, for example, and to investigate signalling pathways through precise spatial and temporal control of their phosphorylation steps...
- Kinetic buffering of cross talk between bacterial two-component sensorsEli S Groban
University of California, San Francisco, 94158, USA
J Mol Biol 390:380-93. 2009..These results support a kinetic model of buffering where both the cognate bifunctional phosphatase activity and the competition between regulator proteins for phosphate prevent cross talk in vivo...
- Automated design of synthetic ribosome binding sites to control protein expressionHoward M Salis
Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA
Nat Biotechnol 27:946-50. 2009..The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems...
- Spatiotemporal control of cell signalling using a light-switchable protein interactionAnselm Levskaya
The Cell Propulsion Lab, UCSF UCB NIH Nanomedicine Development Center, University of California, San Francisco, California 94158 2517, USA
Nature 461:997-1001. 2009....
- Life from informationChristopher A Voigt
Nat Methods 5:27-8. 2008
- Functional evolution and structural conservation in chimeric cytochromes p450: calibrating a structure-guided approachChristopher R Otey
Biochemistry and Molecular Biophysics Option, Mail Code 210 41, Pasadena, CA 91125, USA
Chem Biol 11:309-18. 2004..These results suggest that SCHEMA-guided recombination can be used to generate diverse p450s for exploring function evolution within the p450 structural framework...
- De novo design of biocatalystsDaniel N Bolon
Biochemistry and Molecular Biophysics Option, California Institute of Technology, Mail Code 147 75, Pasadena, California 91125, USA
Curr Opin Chem Biol 6:125-9. 2002..Improved rational design efforts coupled with directed evolution should dramatically improve the scope of de novo enzyme design...