Yoram Vodovotz

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Gender-based reciprocal expression of transforming growth factor-beta1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis
    Pradeep Tyagi
    Department of Surgery, University of Pittsburgh, PA 15213, USA
    J Inflamm (Lond) 6:23. 2009
  2. doi request reprint Systems biology and inflammation
    Yoram Vodovotz
    Center for Inflammation and Regenerative Modeling, McGowan Institute of Regenerative Medicine and Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 662:181-201. 2010
  3. pmc Translational systems approaches to the biology of inflammation and healing
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Immunopharmacol Immunotoxicol 32:181-95. 2010
  4. pmc Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2
    Yuehua Li
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Physiol Regul Integr Comp Physiol 297:R1670-80. 2009
  5. ncbi request reprint The role of initial trauma in the host's response to injury and hemorrhage: insights from a correlation of mathematical simulations and hepatic transcriptomic analysis
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Shock 26:592-600. 2006
  6. ncbi request reprint Systemic inflammation and end organ damage following trauma involves functional TLR4 signaling in both bone marrow-derived cells and parenchymal cells
    Kevin P Mollen
    Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15217, USA
    J Leukoc Biol 83:80-8. 2008
  7. pmc In silico and in vivo approach to elucidate the inflammatory complexity of CD14-deficient mice
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Med 12:88-96. 2006
  8. pmc Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress
    Mallikarjuna R Metukuri
    Department of Surgery, Center for Biologic Imaging, University of Pittsburgh School of Medicine, W943 Biomedical Sciences Tower, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 296:G499-509. 2009
  9. ncbi request reprint Toll-like receptor-4 signaling mediates hepatic injury and systemic inflammation in hemorrhagic shock
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Am Coll Surg 202:407-17. 2006
  10. pmc Inducible protein-10, a potential driver of neurally controlled interleukin-10 and morbidity in human blunt trauma
    Akram M Zaaqoq
    1Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 2Department of Surgery, University of Pittsburgh, Pittsburgh, PA 3Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 4Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, PA 5Department of Rehabilitation Science and Technology, University of Pittsburgh, Pittsburgh, PA 6Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA
    Crit Care Med 42:1487-97. 2014

Collaborators

Detail Information

Publications98

  1. pmc Gender-based reciprocal expression of transforming growth factor-beta1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis
    Pradeep Tyagi
    Department of Surgery, University of Pittsburgh, PA 15213, USA
    J Inflamm (Lond) 6:23. 2009
    ....
  2. doi request reprint Systems biology and inflammation
    Yoram Vodovotz
    Center for Inflammation and Regenerative Modeling, McGowan Institute of Regenerative Medicine and Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 662:181-201. 2010
    ..This chapter reviews the existing, translational applications of computational simulations and related approaches as applied to inflammation...
  3. pmc Translational systems approaches to the biology of inflammation and healing
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Immunopharmacol Immunotoxicol 32:181-95. 2010
    ..This plethora of computational and intertwined experimental/engineering approaches is the cornerstone of Translational Systems Biology approaches for inflammatory diseases...
  4. pmc Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2
    Yuehua Li
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Physiol Regul Integr Comp Physiol 297:R1670-80. 2009
    ....
  5. ncbi request reprint The role of initial trauma in the host's response to injury and hemorrhage: insights from a correlation of mathematical simulations and hepatic transcriptomic analysis
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Shock 26:592-600. 2006
    ..Mathematical simulations and DNA microarrays, both systems biology tools, may provide valuable insight into the complex global physiological interactions that occur in response to trauma and hemorrhagic shock...
  6. ncbi request reprint Systemic inflammation and end organ damage following trauma involves functional TLR4 signaling in both bone marrow-derived cells and parenchymal cells
    Kevin P Mollen
    Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15217, USA
    J Leukoc Biol 83:80-8. 2008
    ..These data demonstrate that functional TLR4 is required in BM-derived cells and parenchymal cells for an optimal inflammatory response to trauma...
  7. pmc In silico and in vivo approach to elucidate the inflammatory complexity of CD14-deficient mice
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Med 12:88-96. 2006
    ....
  8. pmc Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress
    Mallikarjuna R Metukuri
    Department of Surgery, Center for Biologic Imaging, University of Pittsburgh School of Medicine, W943 Biomedical Sciences Tower, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 296:G499-509. 2009
    ..In turn, the upregulation of BNIP3 appears to be one mechanism of hepatocyte cell death and liver damage in these settings...
  9. ncbi request reprint Toll-like receptor-4 signaling mediates hepatic injury and systemic inflammation in hemorrhagic shock
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Am Coll Surg 202:407-17. 2006
    ..We sought to determine the role of lipolysaccharide through use of CD14-/- mice...
  10. pmc Inducible protein-10, a potential driver of neurally controlled interleukin-10 and morbidity in human blunt trauma
    Akram M Zaaqoq
    1Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 2Department of Surgery, University of Pittsburgh, Pittsburgh, PA 3Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 4Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, PA 5Department of Rehabilitation Science and Technology, University of Pittsburgh, Pittsburgh, PA 6Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA
    Crit Care Med 42:1487-97. 2014
    ..We used in silico methods to discern dynamic inflammatory networks that could distinguish systemic inflammation in traumatic spinal cord injury from blunt trauma...
  11. pmc Physiologic responses to severe hemorrhagic shock and the genesis of cardiovascular collapse: can irreversibility be anticipated?
    Hernando Gomez
    Department of Critical Care Medicine, University of Pittsburgh, 3550 Terrace Street, Scaife Hall, Pittsburgh, PA 15261, USA
    J Surg Res 178:358-69. 2012
    ..We hypothesized that vascular tone loss characterizes CC, and that arterial pulse pressure/stroke volume index ratio or vascular tone index (VTI) would identify CC...
  12. pmc Augmenter of liver regeneration (ALR) is a novel biomarker of hepatocellular stress/inflammation: in vitro, in vivo and in silico studies
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    Mol Med 18:1421-9. 2013
    ..ALR may thus serve as a potential diagnostic marker of hepatocellular stress and/or acute inflammatory conditions...
  13. pmc Hemorrhagic shock activates lung endothelial reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase via neutrophil NADPH oxidase
    Meng Xiang
    Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA
    Am J Respir Cell Mol Biol 44:333-40. 2011
    ..Oxidant signaling by neutrophil NADPH oxidase is an important determinant of activation of endothelial NADPH oxidase after HS...
  14. pmc Interacting neuroendocrine and innate and acquired immune pathways regulate neutrophil mobilization from bone marrow following hemorrhagic shock
    Yujian Liu
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 182:572-80. 2009
    ..Blocking this novel signaling axis may present a novel therapeutic target for posttrauma inflammation...
  15. ncbi request reprint Nitric oxide and ionizing radiation synergistically promote apoptosis and growth inhibition of cancer by activating p53
    Tracy Cook
    Department of Surgery and Center for Biologic Imaging, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15232, USA
    Cancer Res 64:8015-21. 2004
    ..Furthermore, the transfer of both p53 and iNOS genes into tumor cells lacking functional p53 enhanced their radioresponsiveness more than transfer of either gene alone...
  16. pmc In vivo, in vitro, and in silico studies suggest a conserved immune module that regulates malaria parasite transmission from mammals to mosquitoes
    Ian Price
    Department of Mathematics, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Theor Biol 334:173-86. 2013
    ..We hypothesize that multiphasic behavior as represented in this model allows the mosquito to balance the conflicting demands of parasite killing and metabolic homeostasis in the face of damaging inflammation. ..
  17. doi request reprint The role of fracture-associated soft tissue injury in the induction of systemic inflammation and remote organ dysfunction after bilateral femur fracture
    Philipp Kobbe
    Department of Orthopedic Surgery, University of Pittsburghm Kaufmann Medical Building, Pittsburgh, PA 15213, USA
    J Orthop Trauma 22:385-90. 2008
    ..We hypothesized that fracture-associated soft tissue injury plays a pivotal role in the induction of systemic inflammation and remote organ dysfunction after fracture...
  18. pmc Hemoadsorption reprograms inflammation in experimental gram-negative septic peritonitis: insights from in vivo and in silico studies
    Rami A Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, United States of America
    Mol Med 18:1366-74. 2012
    ..Hemoadsorption (HA) is an emerging approach to modulate sepsis-induced inflammation. We sought to define the effects of HA on inflammatory compartmentalization in Escherichia coli-induced fibrin peritonitis in rats...
  19. ncbi request reprint Systemic inflammation and remote organ injury following trauma require HMGB1
    Ryan M Levy
    Dept of Surgery, F 1200 PUH, Univ of Pittsburgh, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1538-44. 2007
    ..Collectively, these data demonstrate a critical role for a TLR4-HMGB1 pathway in the initiation of systemic inflammation and end-organ injury following isolated peripheral tissue injury...
  20. doi request reprint Mathematical modeling of posthemorrhage inflammation in mice: studies using a novel, computer-controlled, closed-loop hemorrhage apparatus
    Andres Torres
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Shock 32:172-8. 2009
    ..In conclusion, we have established a hardware/software platform that allows for highly accurate, reproducible, and mathematically predictable HS in mice...
  21. ncbi request reprint In silico models of acute inflammation in animals
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Shock 26:235-44. 2006
    ..Mathematical modeling may provide insights into the complex dynamics of acute inflammation in a manner that can be tested in vivo using many fewer animals than has been possible previously...
  22. ncbi request reprint Systemic inflammation and remote organ damage following bilateral femur fracture requires Toll-like receptor 4
    Ryan M Levy
    Department of Surgery, F 1200 PUH, University of Pittsburgh, 200 Lothrop St, Pittsburgh, PA 15217, USA
    Am J Physiol Regul Integr Comp Physiol 291:R970-6. 2006
    ..Application of these findings in an evolutionary context suggests that multicellular organisms have evolved to use the same pattern recognition receptor for surviving traumatic and infectious challenges...
  23. pmc Central role for MCP-1/CCL2 in injury-induced inflammation revealed by in vitro, in silico, and clinical studies
    Cordelia Ziraldo
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America Department of Computational and Systems Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America Joint Carnegie Mellon University University of Pittsburgh Ph D Program in Computational Biology, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e79804. 2013
    ....
  24. ncbi request reprint Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling
    Jie Fan
    Department of Surgery, School of Medicine, University of Pittsburgh, and Division of Pediatric Surgery, Children s Hospital of Pittsburgh, PA 15213, USA
    J Immunol 178:6573-80. 2007
    ..Thus, PMN NAD(P)H oxidase activation, induced by HS/R and as mediated by HMGB1/TLR4 signaling, is an important mechanism responsible for PMN-mediated inflammation and organ injury after hemorrhage...
  25. doi request reprint Local exposure of bone components to injured soft tissue induces Toll-like receptor 4-dependent systemic inflammation with acute lung injury
    Philipp Kobbe
    Department of Orthopaedic Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Shock 30:686-91. 2008
    ..These findings suggest that bone components contribute to systemic inflammation and acute lung injury after long bone fractures via TLR-4 signaling and support the notion of a central role for TLR-4 in sensing tissue damage...
  26. pmc Biosimulation of acute phonotrauma: an extended model
    Nicole Y K Li
    Department of Surgery, University of Wisconsin Madison, Madison, Wisconsin, USA
    Laryngoscope 121:2418-28. 2011
    ..The central aim of the present study was to investigate the utility of mechanistic computational modeling of inflammation and healing to address personalized therapy for patients with acute phonotrauma...
  27. ncbi request reprint The acute inflammatory response in diverse shock states
    Carson C Chow
    Department of Mathematics, University of Pittsburgh, Pittsburgh, Pensylvania 15261, USA
    Shock 24:74-84. 2005
    ....
  28. pmc Early airway pressure release ventilation prevents ARDS-a novel preventive approach to lung injury
    Shreyas Roy
    Cardiopulmonary and Critical Care Laboratory, Department of Surgery, Upstate Medical University, Syracuse, NY, USA
    Shock 39:28-38. 2013
    ..Protective ventilation could change the clinical paradigm from supportive care for ARDS with LTV ventilation to preventing development of ARDS with APRV...
  29. pmc A dynamic view of trauma/hemorrhage-induced inflammation in mice: principal drivers and networks
    Qi Mi
    Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e19424. 2011
    ..We utilized multiplexing cytokine analysis coupled with data-driven modeling to gain a systems perspective into T/HS...
  30. pmc A simple, rapid, and convenient Luminex-compatible method of tissue isolation
    Derek Barclay
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Clin Lab Anal 22:278-81. 2008
    ..This method was compared with flash-freezing in both mouse liver and human debrided wound specimens, and may be of utility in other clinical settings...
  31. pmc An adequately robust early TNF-alpha response is a hallmark of survival following trauma/hemorrhage
    Rajaie Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 4:e8406. 2009
    ..Trauma/hemorrhagic shock (T/HS) results in cytokine-mediated acute inflammation that is generally considered detrimental...
  32. pmc Combined in silico, in vivo, and in vitro studies shed insights into the acute inflammatory response in middle-aged mice
    Rami A Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 8:e67419. 2013
    ....
  33. doi request reprint Roles of dendritic cells in murine hepatic warm and liver transplantation-induced cold ischemia/reperfusion injury
    Matthew Zhang
    Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Hepatology 57:1585-96. 2013
    ..When Flt3L KO liver was transplanted into WT mice, ALT levels were significantly higher than in WT to WT LTx, with enhanced hepatic necrosis and neutrophil infiltration, indicating a protective role of liver-resident DC...
  34. pmc Hepatic stellate cells undermine the allostimulatory function of liver myeloid dendritic cells via STAT3-dependent induction of IDO
    Tina L Sumpter
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 189:3848-58. 2012
    ..In conclusion, by promoting IDO expression, HSCs may act as potent regulators of liver mDCs and function to maintain hepatic homeostasis and tolerogenicity...
  35. pmc A two-compartment mathematical model of endotoxin-induced inflammatory and physiologic alterations in swine
    Gary Nieman
    Department of Surgery, Upstate Medical University, Syracuse, NY, USA
    Crit Care Med 40:1052-63. 2012
    ..To gain insights into individual variations in acute inflammation and physiology...
  36. pmc Hypoxia-induced overexpression of BNIP3 is not dependent on hypoxia-inducible factor 1α in mouse hepatocytes
    Rajaie A Namas
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Shock 36:196-202. 2011
    ..These results suggest that HIF-1α does not mediate the hypoxia-induced upregulation of BNIP3 in mouse hepatocytes in vitro and possibly in the liver in vivo...
  37. pmc Transcriptomic response of murine liver to severe injury and hemorrhagic shock: a dual-platform microarray analysis
    Rebecca D Edmonds
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Physiol Genomics 43:1170-83. 2011
    ..This is the first study to examine the postinjury response using dual-platform microarray analysis, revealing responses that may enable novel therapies or diagnostics...
  38. doi request reprint Patterns of cytokine release and evolution of remote organ dysfunction after bilateral femur fracture
    Philipp Kobbe
    Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Shock 30:43-7. 2008
    ....
  39. ncbi request reprint Neutrophil NAD(P)H oxidase is required for hemorrhagic shock-enhanced TLR2 up-regulation in alveolar macrophages in response to LPS
    Jie Fan
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15240, USA
    Shock 28:213-8. 2007
    ..Thus, PMN nicotinamide adenine dinucleotide phosphate (reduced form) oxidase sensitizes hemorrhagic shock-primed AM to LPS, at least in part via enhancing IL-1 receptor associated kinase 4 activity...
  40. ncbi request reprint Inflammatory modulation of hepatocyte apoptosis by nitric oxide: in vivo, in vitro, and in silico studies
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Curr Mol Med 4:753-62. 2004
    ..Our studies suggest that modulation of iron, oxygen, and superoxide may dictate whether NO* is hepatoprotective or hepatotoxic...
  41. ncbi request reprint A reduced mathematical model of the acute inflammatory response II. Capturing scenarios of repeated endotoxin administration
    Judy Day
    Department of Mathematics, 301 Thackeray, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Theor Biol 242:237-56. 2006
    ....
  42. pmc Endotoxin uptake in mouse liver is blocked by endotoxin pretreatment through a suppressor of cytokine signaling-1-dependent mechanism
    Melanie J Scott
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Hepatology 49:1695-708. 2009
    ..In the same samples, ubiquitinated TIRAP was detected after 4 hours and up to 8 hours after LPS stimulation, but not at later times...
  43. pmc Insights into the Role of Chemokines, Damage-Associated Molecular Patterns, and Lymphocyte-Derived Mediators from Computational Models of Trauma-Induced Inflammation
    Rami A Namas
    1 Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania
    Antioxid Redox Signal 23:1370-87. 2015
    ..The complex interplay between inflammation and physiology in critical illness remains a challenge for translational research, including the extrapolation to human disease from animal models...
  44. pmc Impact of Injury Severity on Dynamic Inflammation Networks Following Blunt Trauma
    Khalid Almahmoud
    Department of Surgery and Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, and Department of Critical Care Medicine, University of Pittsburgh, Pennsylvania
    Shock 44:101-9. 2015
    ..We hypothesized that dynamic networks of systemic inflammation manifest differently as a function of injury severity in human blunt trauma...
  45. pmc Temporal Patterns of Circulating Inflammation Biomarker Networks Differentiate Susceptibility to Nosocomial Infection Following Blunt Trauma in Humans
    Rami A Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA Department of Internal Medicine, Division of Rheumatology, Wayne State University, Detroit, MI Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, PA
    Ann Surg 263:191-8. 2016
    ..We hypothesized that early circulating biomarker patterns following trauma would correlate with sustained immune dysregulation associated with NI and remote organ failure...
  46. ncbi request reprint A DNA microarray study of nitric oxide-induced genes in mouse hepatocytes: implications for hepatic heme oxygenase-1 expression in ischemia/reperfusion
    Ruben Zamora
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Nitric Oxide 7:165-86. 2002
    ....
  47. ncbi request reprint Adenoviral gene transfer of the human inducible nitric oxide synthase gene enhances the radiation response of human colorectal cancer associated with alterations in tumor vascularity
    Zifa Wang
    Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15232, USA
    Cancer Res 64:1386-95. 2004
    ..These radiosensitizing effects of AdiNOS occurred at low infection efficiency (4% of tumor infected), indicating a significant bystander effect...
  48. ncbi request reprint The role of hepatic type 1 plasminogen activator inhibitor (PAI-1) during murine hemorrhagic shock
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, S411 B South BST, Pittsburgh, PA 15261, USA
    Hepatology 42:390-9. 2005
    ..In conclusion, PAI-1 protein is a negative effector of hepatic damage after HS-R through its influence on classic regulators of hepatic growth, as opposed to its role in fibrinolysis...
  49. pmc Translational systems biology of inflammation
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS Comput Biol 4:e1000014. 2008
    ..Future advances in understanding complex medical problems are highly dependent on methodological advances and integration of the computational systems biology community with biologists and clinicians...
  50. pmc Computational Analysis Supports an Early, Type 17 Cell-Associated Divergence of Blunt Trauma Survival and Mortality
    Andrew Abboud
    1Department of Surgery, University of Pittsburgh, Pittsburgh, PA 2Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, PA 3Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 4Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 5Division of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 6Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, Pittsburgh, PA
    Crit Care Med 44:e1074-e1081. 2016
    ..We hypothesized that this divergence was due to different trajectories of systemic inflammation and utilized computational analyses to define these differences...
  51. pmc Elevated Admission Base Deficit Is Associated with a Complex Dynamic Network of Systemic Inflammation Which Drives Clinical Trajectories in Blunt Trauma Patients
    Othman Abdul-Malak
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Mediators Inflamm 2016:7950374. 2016
    ..These results suggest that elevated admission BD is associated with differential immune/inflammatory pathways, which subsequently could predispose patients to follow a complicated clinical course...
  52. pmc Individual-specific principal component analysis of circulating inflammatory mediators predicts early organ dysfunction in trauma patients
    Rami A Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213 Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219
    J Crit Care 36:146-153. 2016
    ....
  53. ncbi request reprint Characterization of the expression of inducible nitric oxide synthase in rat and human liver during hemorrhagic shock
    Joy L Collins
    Department of Surgery, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Shock 19:117-22. 2003
    ..Collectively, these results suggest that hepatic iNOS expression, associated with peroxisomal localization, is an early molecular response to HS in experimental animals and possibly in human patients with trauma with HS...
  54. doi request reprint The central role of arginine catabolism in T-cell dysfunction and increased susceptibility to infection after physical injury
    Xinmei Zhu
    Departments of Surgery Departments of Microbiology, Immunology, and Parasitology, Stanley S Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA Immunology Microbiology and Molecular Genetics Critical Care Medicine, University of Pittsburgh School of Medicine, University of Pittsburgh, Pittsburgh, PA and Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine
    Ann Surg 259:171-8. 2014
    ..To explore the hypothesis that decreased arginine availability by myeloid-derived suppressor cells (MDSCs) is a cause of T-cell dysfunction after physical injury (PI)...
  55. pmc Selective expansion of allogeneic regulatory T cells by hepatic stellate cells: role of endotoxin and implications for allograft tolerance
    Anil Dangi
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 188:3667-77. 2012
    ..These data implicate endotoxin-stimulated HSCs as important immune regulators in liver transplantation by inducing selective expansion of tolerance-promoting Tregs and reducing inflammation and alloimmunity...
  56. ncbi request reprint Autologous nitric oxide protects mouse and human keratinocytes from ultraviolet B radiation-induced apoptosis
    Richard Weller
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Am J Physiol Cell Physiol 284:C1140-8. 2003
    ..These results demonstrate an antiapoptotic role for NO in keratinocytes, mediated by cGMP, and indicate an antiapoptotic role for both eNOS and iNOS in skin damage induced by UVB...
  57. pmc Models of lower extremity damage in mice: time course of organ damage and immune response
    Christoph L Menzel
    Department of Orthopedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
    J Surg Res 166:e149-56. 2011
    ..This follow-up study tests the hypothesis that inflammatory responses are comparable between a novel trauma model ("pseudofracture", PFx) and a bilateral femur fracture (BFF) model...
  58. pmc Translational systems biology: introduction of an engineering approach to the pathophysiology of the burn patient
    Gary An
    Department of Surgery, Northwestern University, Chicago, IL 60611, USA
    J Burn Care Res 29:277-85. 2008
    ....
  59. pmc Challenges and rewards on the road to translational systems biology in acute illness: four case reports from interdisciplinary teams
    Gary An
    Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
    J Crit Care 22:169-75. 2007
    ..However, the development of these groups must overcome domain-specific barriers to communication and understanding...
  60. ncbi request reprint Nitric oxide-mediated inhibition of caspase-dependent T lymphocyte proliferation
    Raja S Mahidhara
    Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    J Leukoc Biol 74:403-11. 2003
    ..Previous studies demonstrated inhibition of apoptosis through S-nitrosylation of caspases; the present studies extend this effect to inhibition of caspase-dependent T cell proliferation...
  61. ncbi request reprint Hepatocytes enhance effects of lipopolysaccharide on liver nonparenchymal cells through close cell interactions
    Melanie J Scott
    Department of Surgery Laboratories, Montefiore University Hospital, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Shock 23:453-8. 2005
    ..Thus, interaction between hepatocytes and NPC are critical for cytokine secretion by NPC...
  62. ncbi request reprint Early growth response 1 mediates the systemic and hepatic inflammatory response initiated by hemorrhagic shock
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA, and Department of Hepatobiliary Surgery, General Hospital of Beijing Military District, DongSi, Beijing, China
    Shock 27:157-64. 2007
    ..Our results showing a blunted inflammatory response in Egr-1 mice provides evidence that Egr-1 functions as a proximal signal transduction mechanism responding to shock by amplifying the systemic inflammatory response...
  63. doi request reprint An ensemble of models of the acute inflammatory response to bacterial lipopolysaccharide in rats: results from parameter space reduction
    Silvia Daun
    Department of Critical Care Medicine, School of Medicine, University of Pittsburgh, Scaife 606B, 3550 Terrace St, Pittsburgh, PA 15261, USA
    J Theor Biol 253:843-53. 2008
    ....
  64. pmc Analysis of serum inflammatory mediators identifies unique dynamic networks associated with death and spontaneous survival in pediatric acute liver failure
    Nabil Azhar
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 8:e78202. 2013
    ..We hypothesized that specific, dynamic, and measurable patterns of immune/inflammatory dysregulation will correlate with outcomes in PALF...
  65. pmc In silico modeling: methods and applications to trauma and sepsis
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Crit Care Med 41:2008-14. 2013
    ..To familiarize clinicians with advances in computational disease modeling applied to trauma and sepsis...
  66. pmc Identification of a novel pathway of transforming growth factor-β1 regulation by extracellular NAD+ in mouse macrophages: in vitro and in silico studies
    Ruben Zamora
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 287:31003-14. 2012
    ..Thus, in vitro and in silico evidence points to NAD(+) as a novel modulator of TGF-β1...
  67. pmc Sepsis: Something old, something new, and a systems view
    Rami Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Crit Care 27:314.e1-11. 2012
    ..We highlight the translational impact of computational modeling and other complex systems approaches as applied to sepsis, including in silico clinical trials, patient-specific models, and complexity-based assessments of physiology...
  68. pmc Biosimulation of inflammation and healing in surgically injured vocal folds
    Nicole Y K Li
    Department of Communication Science and Disorders, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Ann Otol Rhinol Laryngol 119:412-23. 2010
    ..The current study is part of research endeavors aimed at applying systems biology approaches to address the complex biological processes involved in the pathogenesis of vocal fold scarring and other lesions affecting the larynx...
  69. ncbi request reprint Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation
    Jie Fan
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
    Am J Physiol Lung Cell Mol Physiol 290:L738-L746. 2006
    ..Thus TLR2 expression in AMphi, signaled by TLR4 and regulated by shock-activated PMN, is an important positive-feedback mechanism responsible for shock-primed PMN infiltration into the lung after primary PMN sequestration...
  70. ncbi request reprint 'Til cell death do us part: nitric oxide and mechanisms of hepatotoxicity
    Peter K M Kim
    Department of Surgery, University of Pittsburgh Medical School, NW607 MUH, 3459 Fifth Avenue, Pittsburgh, PA 15213, USA
    Biol Chem 385:11-5. 2004
    ..Insights that we and others have gained from the liver under pathologic conditions of stress can be applied to the understanding of cellular death mechanisms in other organs and tissues...
  71. ncbi request reprint Peroxisomal localization of inducible nitric oxide synthase in hepatocytes
    Donna Beer Stolz
    Department of Cell Biology and Physiology, University of Pittsburgh Medical School, Pittsburgh, PA 12561, USA
    Hepatology 36:81-93. 2002
    ..In conclusion, these studies establish the peroxisome as a site of iNOS localization in hepatocytes and show a relationship between iNOS up-regulation and decreased expression of catalase...
  72. ncbi request reprint Agent-based model of inflammation and wound healing: insights into diabetic foot ulcer pathology and the role of transforming growth factor-beta1
    Qi Mi
    Department of Mathematics, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Wound Repair Regen 15:671-82. 2007
    ..reduced TGF-beta1). In silico methods may elucidate mechanisms of and suggest therapies for aberrant skin healing...
  73. ncbi request reprint A role for angiotensin II in the activation of extracellular signal-regulated kinases in the liver during hemorrhagic shock
    Carol A McCloskey
    Department of Surgery, University of Pittsburgh Medical Center, Presbyterian University Hospital, Pennsylvania 15213, USA
    Shock 20:316-9. 2003
    ..Together, these data suggest that angiotensin II is an important stimulus for ERK 1/2 activation in the liver during HS...
  74. pmc A patient-specific in silico model of inflammation and healing tested in acute vocal fold injury
    Nicole Y K Li
    Department of Communication Science and Disorders, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 3:e2789. 2008
    ..We propose that this translational application of computational modeling could be used to design patient-specific therapies for the larynx, and will serve as a paradigm for future extension to other clinical domains...
  75. ncbi request reprint Intestinal and hepatic expression of BNIP3 in necrotizing enterocolitis: regulation by nitric oxide and peroxynitrite
    Ruben Zamora
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Am J Physiol Gastrointest Liver Physiol 289:G822-30. 2005
    ....
  76. pmc Mechanistic simulations of inflammation: current state and future prospects
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Math Biosci 217:1-10. 2009
    ..Here we discuss the state of this emerging field. We note several common features of inflammation models, as well as challenges and prospects for future studies...
  77. pmc Activation of latent transforming growth factor-beta1 by nitric oxide in macrophages: role of soluble guanylate cyclase and MAP kinases
    Mallikarjuna Reddy Metukuri
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Wound Repair Regen 17:578-88. 2009
    ....
  78. pmc Multiplex analysis of urinary cytokine levels in rat model of cyclophosphamide-induced cystitis
    Marc C Smaldone
    Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Urology 73:421-6. 2009
    ..In the present study, we subjected rat urine to multiplex cytokine analysis in an attempt to identify an inflammatory signature of the temporal course of cyclophosphamide (CYP)-induced cystitis...
  79. pmc Role of toll-like receptors in changes in gene expression and NF-kappa B activation in mouse hepatocytes stimulated with lipopolysaccharide
    Shubing Liu
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Infect Immun 70:3433-42. 2002
    ..Thus, hepatocytes respond to nanogram concentrations of LPS through a TLR4 response pathway...
  80. pmc The microenvironment in hepatocyte regeneration and function in rats with advanced cirrhosis
    Liping Liu
    Department of Surgery, University of Nebraska Medical Center, Omaha, NE, USA
    Hepatology 55:1529-39. 2012
    ..Both, however, recovered more than 2 months after transplantation, indicating that either mature hepatocytes or a subpopulation of adult stem cells are capable of full recovery in severe cirrhosis...
  81. pmc Evidence-based modeling of critical illness: an initial consensus from the Society for Complexity in Acute Illness
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Crit Care 22:77-84. 2007
    ..Accordingly, SCAI identified a need for and carried out a critical appraisal of DMM as currently used in the setting of acute illness...
  82. ncbi request reprint In silico design of clinical trials: a method coming of age
    Gilles Clermont
    Department of Critical Care Medicine and Clinical Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Crit Care Med 32:2061-70. 2004
    ..To determine the feasibility and potential usefulness of mathematical models in evaluating immunomodulatory strategies in clinical trials of severe sepsis...
  83. pmc Translational systems biology of inflammation and healing
    Yoram Vodovotz
    Department of Surgery Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Wound Repair Regen 18:3-7. 2010
    ..We suggest that computational simulations are the cornerstone of Translational Systems Biology approaches for inflammatory diseases...
  84. ncbi request reprint Thresholded area over the curve of spectrometric tissue oxygen saturation as an indicator of volume resuscitability in porcine hemorrhagic shock
    Sven Zenker
    Center for Inflammation and Regenerative Modeling CIRM, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Trauma 63:573-8; discussion 578-80. 2007
    ..We therefore explored the value of peripheral muscle Sto2 in predicting systemic responsiveness to colloid volume resuscitation in a porcine model of hemorrhagic shock...
  85. pmc A simple mathematical model of signaling resulting from the binding of lipopolysaccharide with Toll-like receptor 4 demonstrates inherent preconditioning behavior
    Beatrice Rivière
    Department of Mathematics, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Math Biosci 217:19-26. 2009
    ....
  86. pmc An exploratory pathways analysis of temporal changes induced by spinal cord injury in the rat bladder wall: insights on remodeling and inflammation
    Silvia Wognum
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 4:e5852. 2009
    ..We sought to characterize the complex pathways of remodeling, inflammation, and infection in the urinary bladder at the level of the transcriptome in a rat model of SCI, using pathways analysis bioinformatics...
  87. pmc Translational systems biology and voice pathophysiology
    Nicole Y K Li
    Department of Communication Science and Disorders, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Laryngoscope 120:511-5. 2010
    ..The purpose of the article is: 1) to review the pros and cons of EBM, and 2) to discuss the alternative systems biology method and present its utility in clinical voice research...
  88. ncbi request reprint Overexpression of the human inducible nitric oxide synthase gene enhances radiation-induced apoptosis in colorectal cancer cells via a caspase-dependent mechanism
    Peter Chung
    Department of Surgery, University of Pittsburgh School of Medicine, Cancer Pavillion, 5150 Centre Avenue, Rm 438, Pittsburgh, PA 15232, USA
    Nitric Oxide 8:119-26. 2003
    ..Gene transfer of iNOS holds much promise as a potential radiosensitizer of cancer cells since it increases apoptosis in an additive manner with radiation...
  89. pmc The nitric oxide donor S-nitrosoglutathione reduces apoptotic primary liver cell loss in a three-dimensional perfusion bioreactor culture model developed for liver support
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15219 3130, USA
    Tissue Eng Part A 16:861-6. 2010
    ....
  90. pmc Cross-talk between nitric oxide and transforming growth factor-beta1 in malaria
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Curr Mol Med 4:787-97. 2004
    ..Bloodfeeding by Anopheles, therefore, results in a juxtaposition of evolutionarily conserved mosquito and mammalian TGF-beta superfamily homologs that may influence transmission dynamics of Plasmodium in endemic regions...
  91. pmc Mammalian target of rapamycin inhibition and alloantigen-specific regulatory T cells synergize to promote long-term graft survival in immunocompetent recipients
    Giorgio Raimondi
    Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 184:624-36. 2010
    ..These findings delineate combination of transient mammalian target of Rapa inhibition with appropriate AAsTreg selection as an effective approach to promote long-term organ graft survival...
  92. ncbi request reprint Mathematical models of the acute inflammatory response
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Curr Opin Crit Care 10:383-90. 2004
    ..There is a growing recognition that the complexity of the acute inflammatory response precludes the efficient development of therapies for sepsis and multiple organ failure until systems approaches are brought to bear on this problem...
  93. ncbi request reprint Deciphering the complexity of acute inflammation using mathematical models
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, and Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA
    Immunol Res 36:237-45. 2006
    ..Modeling may help in understanding the complex interactions among the components of inflammation and response to stress, and therefore aid in the development of novel therapies and diagnostics...
  94. ncbi request reprint Nitric oxide and wound repair: role of cytokines?
    Ann Schwentker
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Nitric Oxide 7:1-10. 2002
    ..In this review, we summarize the history and present state of research on the role of NO in wound healing within the framework of modulation of cytokines...
  95. ncbi request reprint A reduced mathematical model of the acute inflammatory response: I. Derivation of model and analysis of anti-inflammation
    Angela Reynolds
    Department of Mathematics, 301 Thackeray, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Theor Biol 242:220-36. 2006
    ..Further, we offer a clinical correlation between model predictions and potential therapeutic interventions based on modulation of immunity by anti-inflammatory agents...
  96. ncbi request reprint The dynamics of acute inflammation
    Rukmini Kumar
    Departments of Physics and Astronomy, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Theor Biol 230:145-55. 2004
    ..We suggest that the clinical condition of sepsis can arise from several distinct physiological states, each of which requires a different treatment approach...
  97. ncbi request reprint Mechanisms of hepatoprotection by nitric oxide
    Yinna Wang
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Ann N Y Acad Sci 962:415-22. 2002
    ..Our preliminary studies also suggest that NO can modulate apoptosis-related genes in a manner consistent with an antiapoptotic effect. This review focuses on these molecular mechanisms of cytoprotection by NO...
  98. ncbi request reprint Transforming growth factor-beta in critical illness
    Ruben Zamora
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Crit Care Med 33:S478-81. 2005