GERARDO RAUL VASTA

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. ncbi request reprint Innate immunity in the Aegean: ancient pathways for today's survival
    G R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Dev Comp Immunol 26:217-25. 2002
  2. ncbi request reprint The primary structure and carbohydrate specificity of a beta-galactosyl-binding lectin from toad (Bufo arenarum Hensel) ovary reveal closer similarities to the mammalian galectin-1 than to the galectin from the clawed frog Xenopus laevis
    H Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
    J Biol Chem 271:33083-94. 1996
  3. ncbi request reprint Galectins in teleost fish: Zebrafish (Danio rerio) as a model species to address their biological roles in development and innate immunity
    Gerardo R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD, 21202, USA
    Glycoconj J 21:503-21. 2004
  4. pmc Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
    Keiko Saito
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt St, Baltimore, MD 21202, USA
    Appl Environ Microbiol 73:6410-20. 2007
  5. ncbi request reprint Biological roles of lectins in innate immunity: molecular and structural basis for diversity in self/non-self recognition
    Gerardo R Vasta
    University of Maryland Biotechnology Institute, Center of Marine Biotechnology, 701 East Pratt Street, Baltimore, MD 21202, USA
    Adv Exp Med Biol 598:389-406. 2007
  6. ncbi request reprint Structural and functional diversity of lectin repertoires in invertebrates, protochordates and ectothermic vertebrates
    Gerardo R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
    Curr Opin Struct Biol 14:617-30. 2004
  7. ncbi request reprint C-type lectins and galectins mediate innate and adaptive immune functions: their roles in the complement activation pathway
    G R Vasta
    Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202, USA
    Dev Comp Immunol 23:401-20. 1999
  8. pmc Roles of galectins in infection
    Gerardo R Vasta
    University of Maryland Biotechnology Institute, Center of Marine Biotechnology, Columbus Center, Baltimore, 21202, USA
    Nat Rev Microbiol 7:424-38. 2009
  9. ncbi request reprint Biochemical and molecular characterization of galectins from zebrafish (Danio rerio): notochord-specific expression of a prototype galectin during early embryogenesis
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Glycobiology 14:219-32. 2004
  10. ncbi request reprint Novel carbohydrate specificity of the 16-kDa galectin from Caenorhabditis elegans: binding to blood group precursor oligosaccharides (type 1, type 2, Talpha, and Tbeta) and gangliosides
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Glycobiology 12:451-61. 2002

Collaborators

  • Hafiz Ahmed
  • Mario A Bianchet
  • Julie D Gauthier
  • Jun Hirabayashi
  • PARTHA BANERJEE
  • Eric J Schott
  • José A Fernández-Robledo
  • Wolf T Pecher
  • Keiko Saito
  • Tomas Drgon
  • Mohammad R Alavi
  • José A F Robledo
  • Danara N Krupatkina
  • José Antonio F Robledo
  • Anita C Wright
  • Satoshi Tasumi
  • Eric W Odom
  • Abelardo M Silva
  • Laura Roth
  • Zhuoer Lin
  • Sean T Berg
  • Jana Drgonova
  • Natalia Mercer
  • Daniel E Terlizzi
  • Brent Whitaker
  • Federico Argemi
  • Patrick M Gillevet
  • Masoumeh Sikaroodi
  • Pascal Courville
  • Mathieu F M Cellier
  • Florence Okafor
  • Patricia A Nunes
  • M Leonor Cancela

Detail Information

Publications31

  1. ncbi request reprint Innate immunity in the Aegean: ancient pathways for today's survival
    G R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Dev Comp Immunol 26:217-25. 2002
    ....
  2. ncbi request reprint The primary structure and carbohydrate specificity of a beta-galactosyl-binding lectin from toad (Bufo arenarum Hensel) ovary reveal closer similarities to the mammalian galectin-1 than to the galectin from the clawed frog Xenopus laevis
    H Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
    J Biol Chem 271:33083-94. 1996
    ..laevis 16-kDa galectin, clearly constitute distinct subgroups in the extant amphibian taxa and may have diverged early in the evolution of chordate lineages...
  3. ncbi request reprint Galectins in teleost fish: Zebrafish (Danio rerio) as a model species to address their biological roles in development and innate immunity
    Gerardo R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD, 21202, USA
    Glycoconj J 21:503-21. 2004
    ....
  4. pmc Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
    Keiko Saito
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt St, Baltimore, MD 21202, USA
    Appl Environ Microbiol 73:6410-20. 2007
    ..The addition of ammonia, urea, nitrate, phosphate, or surprisingly, spent fish aquarium water had no effect...
  5. ncbi request reprint Biological roles of lectins in innate immunity: molecular and structural basis for diversity in self/non-self recognition
    Gerardo R Vasta
    University of Maryland Biotechnology Institute, Center of Marine Biotechnology, 701 East Pratt Street, Baltimore, MD 21202, USA
    Adv Exp Med Biol 598:389-406. 2007
  6. ncbi request reprint Structural and functional diversity of lectin repertoires in invertebrates, protochordates and ectothermic vertebrates
    Gerardo R Vasta
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, Maryland 21202, USA
    Curr Opin Struct Biol 14:617-30. 2004
    ....
  7. ncbi request reprint C-type lectins and galectins mediate innate and adaptive immune functions: their roles in the complement activation pathway
    G R Vasta
    Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202, USA
    Dev Comp Immunol 23:401-20. 1999
    ..Therefore, despite being 'new' to the textbooks, experimental evidence indicates that this pathway is ancient, and has been conserved intact throughout its evolution...
  8. pmc Roles of galectins in infection
    Gerardo R Vasta
    University of Maryland Biotechnology Institute, Center of Marine Biotechnology, Columbus Center, Baltimore, 21202, USA
    Nat Rev Microbiol 7:424-38. 2009
    ..This Review discusses the role of galectins in microbial infection, with particular emphasis on adaptations of pathogens to evasion or subversion of host galectin-mediated immune responses...
  9. ncbi request reprint Biochemical and molecular characterization of galectins from zebrafish (Danio rerio): notochord-specific expression of a prototype galectin during early embryogenesis
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Glycobiology 14:219-32. 2004
    ..The distinct temporal and spatial patterns of expression of members of the zebrafish galectin repertoire suggest that each may play distinct biological roles during early embryogenesis...
  10. ncbi request reprint Novel carbohydrate specificity of the 16-kDa galectin from Caenorhabditis elegans: binding to blood group precursor oligosaccharides (type 1, type 2, Talpha, and Tbeta) and gangliosides
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Glycobiology 12:451-61. 2002
    ....
  11. ncbi request reprint Transfection of the protozoan parasite Perkinsus marinus
    José A Fernández-Robledo
    University of Maryland Biotechnology Institute, Center of Marine Biotechnology, 701 E Pratt Street, Suite 236, Baltimore, MD 21202 3101, USA
    Mol Biochem Parasitol 157:44-53. 2008
    ....
  12. doi request reprint Knockdown of a galectin-1-like protein in zebrafish (Danio rerio) causes defects in skeletal muscle development
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Glycoconj J 26:277-83. 2009
    ....
  13. pmc Characterization of ichthyocidal activity of Pfiesteria piscicida: dependence on the dinospore cell density
    Tomas Drgon
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt St, Baltimore, MD 21202, USA
    Appl Environ Microbiol 71:519-29. 2005
    ..and (ii) that the ichthyocidal activity of Pfiesteria spp. is mostly due to direct interactions of the zoospores with fish skin and gill epithelia rather than to soluble factors...
  14. ncbi request reprint Gene organization and expression of the divalent cation transporter Nramp in the protistan parasite Perkinsus marinus
    José Antonio F Robledo
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202 3101, USA
    J Parasitol 90:1004-14. 2004
    ..marinus sequence and the rest of the archetype Nramp group. Preliminary studies suggest that expression of PmNramp in in vitro-cultured P. marinus trophozoites is modulated by metals and by exogenous oxidative stress...
  15. ncbi request reprint Differential expression of galectins in normal, benign and malignant prostate epithelial cells: silencing of galectin-3 expression in prostate cancer by its promoter methylation
    Hafiz Ahmed
    University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
    Biochem Biophys Res Commun 358:241-6. 2007
    ..The methylation of gal3 promoter may constitute a powerful tool for early diagnosis of prostate cancer...
  16. ncbi request reprint Identification of a second rRNA gene unit in the Perkinsus andrewsi genome
    Wolf T Pecher
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, Baltimore, Maryland 21202, USA
    J Eukaryot Microbiol 51:234-45. 2004
    ..Each rRNA gene unit is arranged in a "head-to-tail" tandem repeat. This is the first report demonstrating two distinct rRNA units in a Perkinsus species...
  17. ncbi request reprint Development of an in vitro clonal culture and characterization of the rRNA gene cluster of Perkinsus atlanticus, a protistan parasite of the clam Tapes decussatus
    José A F Robledo
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, Baltimore 21202, USA
    J Eukaryot Microbiol 49:414-22. 2002
    ..This Perkinsus "genus-specific" PCR-based assay complements the species-specific assays developed earlier and strengthen the detection of Perkinsus species for which specific detection assays are not yet available...
  18. pmc Unlike mammalian GRIFIN, the zebrafish homologue (DrGRIFIN) represents a functional carbohydrate-binding galectin
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E Pratt Street, Suite 236, Baltimore, MD 21202, USA
    Biochem Biophys Res Commun 371:350-5. 2008
    ..Therefore, DrGRIFIN is a bona fide galectin family member that in addition to its carbohydrate-binding properties, may also function as a crystallin...
  19. pmc Structure and specificity of a binary tandem domain F-lectin from striped bass (Morone saxatilis)
    Mario A Bianchet
    Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Mol Biol 401:239-52. 2010
    ....
  20. ncbi request reprint Gene organization and homology modeling of two iron superoxide dismutases of the early branching protist Perkinsus marinus
    Eric J Schott
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Gene 309:1-9. 2003
    ..The divergent genomic organizations of two FeSOD genes in the same organism illustrates the complexity of the antioxidant system of even simple, early-branching protists such as P. marinus...
  21. pmc Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the nontranscribed spacer
    Keiko Saito
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
    Appl Environ Microbiol 68:5394-407. 2002
    ..piscicida in water and sediments...
  22. doi request reprint Assessment of the northern distribution range of selected Perkinsus species in eastern oysters (Crassostrea virginica) and hard clams (Mercenaria mercenaria) with the use of PCR-based detection assays
    Wolf T Pecher
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
    J Parasitol 94:410-22. 2008
    ..Interestingly, the prevalence of P. marinus in M. mercenaria was significantly lower than in C. virginica, suggesting that M. mercenaria is not an optimal host for P. marinus...
  23. ncbi request reprint A galectin of unique domain organization from hemocytes of the Eastern oyster (Crassostrea virginica) is a receptor for the protistan parasite Perkinsus marinus
    Satoshi Tasumi
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    J Immunol 179:3086-98. 2007
    ..marinus subverts the host's immune/feeding recognition mechanism to passively gain entry into the hemocytes...
  24. ncbi request reprint cDNA cloning and characterization of two iron superoxide dismutases from the oyster parasite Perkinsus marinus
    Anita C Wright
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Mol Biochem Parasitol 123:73-77. 2002
  25. ncbi request reprint A novel fucose recognition fold involved in innate immunity
    Mario A Bianchet
    Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Nat Struct Biol 9:628-34. 2002
    ..Modification of these residues explains how the different isoforms in serum can provide a diverse pathogen-specific recognition...
  26. ncbi request reprint The PmSOD1 gene of the protistan parasite Perkinsus marinus complements the sod2Delta mutant of Saccharomyces cerevisiae, and directs an iron superoxide dismutase to mitochondria
    Eric J Schott
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore 21202, USA
    Mol Biochem Parasitol 126:81-92. 2003
    ..It also illustrates the potential of complementation-based approaches for further gene discovery and characterization in P. marinus...
  27. ncbi request reprint Characterization of a binary tandem domain F-type lectin from striped bass (Morone saxatilis)
    Eric W Odom
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202, USA
    J Biol Chem 281:1698-713. 2006
    ..In contrast with the amniotes, in which the F-type lectins appear conspicuously absent, the widespread gene duplication in the teleost fish suggests these F-type lectins acquired increasing evolutionary value within this taxon...
  28. ncbi request reprint Superoxide dismutases from the oyster parasite Perkinsus marinus: purification, biochemical characterization, and development of a plate microassay for activity
    Hafiz Ahmed
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Anal Biochem 318:132-41. 2003
    ..The results are discussed in the context of processing of mitochondrially targeted SODs...
  29. ncbi request reprint The protistan parasite Perkinsus marinus is resistant to selected reactive oxygen species
    Eric J Schott
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202, USA
    Exp Parasitol 105:232-40. 2003
    ..Viable P. marinus trophozoites degrade H(2)O(2) in vitro, but lack detectable catalase activity. However, extracts contain an ascorbate dependent peroxidase activity that may contribute to H(2)O(2) removal in vitro and in vivo...
  30. doi request reprint Perkinsus marinus superoxide dismutase 2 (PmSOD2) localizes to single-membrane subcellular compartments
    José A Fernández-Robledo
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 East Pratt Street, Baltimore, MD 21202 3101, USA
    Biochem Biophys Res Commun 375:215-9. 2008
    ..In light of the in silico targeting prediction, the association of PmSOD2 with single-membrane compartments raises interesting questions regarding its organellar targeting, and the nature of a putative relic plastid in Perkinsus species...
  31. doi request reprint Development of an in vitro assay to examine intracellular survival of Perkinsus marinus trophozoites upon phagocytosis by oyster (Crassostrea virginica and Crassostrea ariakensis) hemocytes
    Mohammad R Alavi
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202 3101, USA
    J Parasitol 95:900-7. 2009
    ..marinus genome, EST sequences, and the transfection methodology we recently developed, this assay should significantly contribute to a rigorous identification of the P. marinus genes responsible for its intrahemocytic survival...