Genomes and Genes
Richard L Thompson
Affiliation: University of Cincinnati
- Herpes simplex virus type 1 latency-associated transcript gene promotes neuronal survivalR L Thompson
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati Medical Center, 231 Albert Sabin Way, Cincinnati, Ohio 45267 0524, USA
J Virol 75:6660-75. 2001..Thus, one function of the LAT gene is to protect sensory neurons and enhance the establishment of latency in the PNS...
- Analysis of herpes simplex virus ICP0 promoter function in sensory neurons during acute infection, establishment of latency, and reactivation in vivoR L Thompson
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0524, USA
J Virol 77:12319-30. 2003..These last results strongly suggest that there is a posttranscriptional constraint on the expression of ICP0 protein during reactivation from latency and that this constraint is mediated by LAT...
- The herpes simplex virus type 1 latency associated transcript locus is required for the maintenance of reactivation competent latent infectionsRichard L Thompson
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0524, USA
J Neurovirol 17:552-8. 2011..Here, we report that the latency associated transcript locus of HSV-1 is required for long-term maintenance of reactivation competent latent infections...
- VP16 serine 375 is a critical determinant of herpes simplex virus exit from latency in vivoNancy M Sawtell
Department of Pediatrics, Division of Infectious Diseases, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229 3039, USA
J Neurovirol 17:546-51. 2011....
- Therapeutic implications of new insights into the critical role of VP16 in initiating the earliest stages of HSV reactivation from latencyRichard L Thompson
Department of Molecular Genetics, Microbiology, and Biochemistry, University of Cincinnati School of Medicine, Cincinnati, OH 45267 0524, USA
Future Med Chem 2:1099-105. 2010..Blocking VP16 transactivation would reduce the spread of the virus in the population and, importantly, presumably reduce or prevent the pathological long term chronic inflammation in the nervous system...
- De novo synthesis of VP16 coordinates the exit from HSV latency in vivoRichard L Thompson
Department of Molecular Genetics, Microbiology, and Biochemistry, University of Cincinnati School of Medicine, Cincinnati, Ohio, USA
PLoS Pathog 5:e1000352. 2009..HSV reactivation from latency conforms to a model in which stochastic derepression of the VP16 promoter and expression of VP16 initiates entry into the lytic cycle...
- Herpes simplex virus and the lexicon of latency and reactivation: a call for defining terms and building an integrated collective frameworkNancy M Sawtell
Division of Infectious Diseases, Cincinnati Children s Hospital Medical Center, Cincinnati, OH, USA
F1000Res 5:. 2016..We propose to open a dialogue among investigators for the purpose of updating and clearly defining terms used to describe these processes and to build a collective integrated framework to move our field forward. ..
- A forward phenotypically driven unbiased genetic analysis of host genes that moderate herpes simplex virus virulence and stromal keratitis in miceRichard L Thompson
Department of Molecular Genetics, Microbiology, and Biochemistry, University of Cincinnati College of Medicine, Cincinnati, Ohio, United States of America
PLoS ONE 9:e92342. 2014..To our knowledge this is the first report of a host genetic locus that modulates the severity of both herpetic disease in the nervous system and herpetic stromal keratitis...
- Herpes simplex virus mutant generation and dual-detection methods for gaining insight into latent/lytic cycles in vivoNancy M Sawtell
Division of Infectious Diseases, Department of Pediatrics, Cincinnati Children s Hospital Medical Center, 3333 Burnet Ave, MLC 7017, Cincinnati, OH, 45229 3039, USA
Methods Mol Biol 1144:129-47. 2014..Our strategy and protocols for generating, characterizing, and employing HSV viral promoter/reporter mutants in vivo are provided in this two-part chapter. ..
- Determination, by inductively coupled plasma mass spectrometry, of changes in cellular metal content resulting from herpes simplex virus-1 (HSV-1) infectionKatie DeNicola Cafferky
Department of Chemistry, University of Cincinnati, Mail Location 0172, Cincinnati, OH 45221, USA
Anal Bioanal Chem 387:2037-43. 2007..This work is the first step in the identification of metals pertinent to HSV-1 infection and lays the foundation for future studies concentrating on characterization of these metal-associated or containing molecules...