James A Swenberg

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Dosimetry of N⁶-formyllysine adducts following [¹³C²H₂]-formaldehyde exposures in rats
    Bahar Edrissi
    Department of Biological Engineering, Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States
    Chem Res Toxicol 26:1421-3. 2013
  2. pmc Formaldehyde carcinogenicity research: 30 years and counting for mode of action, epidemiology, and cancer risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Pathol 41:181-9. 2013
  3. ncbi request reprint Biomarkers in toxicology and risk assessment: informing critical dose-response relationships
    James A Swenberg
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 21:253-65. 2008
  4. pmc Endogenous versus exogenous DNA adducts: their role in carcinogenesis, epidemiology, and risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 120:S130-45. 2011
  5. pmc A new LC-MS/MS method for the quantification of endogenous and vinyl chloride-induced 7-(2-Oxoethyl)guanine in sprague-dawley rats
    Esra Mutlu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 25:391-9. 2012
  6. pmc Identification and characterization of 2'-deoxyadenosine adducts formed by isoprene monoepoxides in vitro
    Petra Begemann
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, North Carolina 27599 7431, USA
    Chem Res Toxicol 24:1048-61. 2011
  7. ncbi request reprint Effects of ethylene oxide and ethylene inhalation on DNA adducts, apurinic/apyrimidinic sites and expression of base excision DNA repair genes in rat brain, spleen, and liver
    Ivan Rusyn
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    DNA Repair (Amst) 4:1099-110. 2005
  8. ncbi request reprint Analysis of diepoxide-specific cyclic N-terminal globin adducts in mice and rats after inhalation exposure to 1,3-butadiene
    Gunnar Boysen
    Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Cancer Res 64:8517-20. 2004
  9. pmc Accumulation of true single strand breaks and AP sites in base excision repair deficient cells
    April M Luke
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, USA
    Mutat Res 694:65-71. 2010
  10. ncbi request reprint Molecular dosimetry of N7-(2-hydroxypropyl)guanine in tissues of F344 rats after inhalation exposure to propylene oxide
    Melva N Ríos-Blanco
    Curriculum in Toxicology, Campus Box 7431, Rosenau Hall 253c, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Carcinogenesis 24:1233-8. 2003

Collaborators

Detail Information

Publications96

  1. pmc Dosimetry of N⁶-formyllysine adducts following [¹³C²H₂]-formaldehyde exposures in rats
    Bahar Edrissi
    Department of Biological Engineering, Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, United States
    Chem Res Toxicol 26:1421-3. 2013
    ..1 ppm formaldehyde exposure resulting in one-third of the total load of N⁶-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts...
  2. pmc Formaldehyde carcinogenicity research: 30 years and counting for mode of action, epidemiology, and cancer risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Pathol 41:181-9. 2013
    ....
  3. ncbi request reprint Biomarkers in toxicology and risk assessment: informing critical dose-response relationships
    James A Swenberg
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 21:253-65. 2008
    ..It is also clear, however, that low dose data on mutagenesis are needed for many more chemicals...
  4. pmc Endogenous versus exogenous DNA adducts: their role in carcinogenesis, epidemiology, and risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 120:S130-45. 2011
    ..Finally, it extends the thought process to VC, EO, and additional areas of potential research, pointing out needs, nuances, and potential paths forward to improved understanding that will lead to strong science-based risk assessment...
  5. pmc A new LC-MS/MS method for the quantification of endogenous and vinyl chloride-induced 7-(2-Oxoethyl)guanine in sprague-dawley rats
    Esra Mutlu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 25:391-9. 2012
    ..Studies on the persistence of [(13)C(2)]-7-OEG in adult rats sacrificed 2, 4, and 8 weeks postexposure to [(13)C(2)]-VC demonstrated a half-life of 7-OEG of 4 days in both liver and lung...
  6. pmc Identification and characterization of 2'-deoxyadenosine adducts formed by isoprene monoepoxides in vitro
    Petra Begemann
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, North Carolina 27599 7431, USA
    Chem Res Toxicol 24:1048-61. 2011
    ....
  7. ncbi request reprint Effects of ethylene oxide and ethylene inhalation on DNA adducts, apurinic/apyrimidinic sites and expression of base excision DNA repair genes in rat brain, spleen, and liver
    Ivan Rusyn
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    DNA Repair (Amst) 4:1099-110. 2005
    ..We conclude that accumulation of AP sites is not a likely primary mechanism for mutagenicity and carcinogenicity of EO...
  8. ncbi request reprint Analysis of diepoxide-specific cyclic N-terminal globin adducts in mice and rats after inhalation exposure to 1,3-butadiene
    Gunnar Boysen
    Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Cancer Res 64:8517-20. 2004
    ..This assay should also be readily adaptable to molecular epidemiology studies on 1,3-butadiene-exposed workers...
  9. pmc Accumulation of true single strand breaks and AP sites in base excision repair deficient cells
    April M Luke
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, USA
    Mutat Res 694:65-71. 2010
    ..Furthermore, the Pol β-null cells displayed greater AP site formation than the parental DT40 cells. OTX use represents a facile approach for assessing SSB formation, whose benefits can also be applied to other established SSB assays...
  10. ncbi request reprint Molecular dosimetry of N7-(2-hydroxypropyl)guanine in tissues of F344 rats after inhalation exposure to propylene oxide
    Melva N Ríos-Blanco
    Curriculum in Toxicology, Campus Box 7431, Rosenau Hall 253c, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Carcinogenesis 24:1233-8. 2003
    ....
  11. pmc Solution structures of a DNA dodecamer duplex with and without a cisplatin 1,2-d(GG) intrastrand cross-link: comparison with the same DNA duplex containing an oxaliplatin 1,2-d(GG) intrastrand cross-link
    Yibing Wu
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 7260, USA
    Biochemistry 46:6477-87. 2007
    ..We hypothesize that these conformational differences may be related to the ability of various DNA repair proteins, DNA binding proteins, and DNA polymerases to discriminate between cisplatin-GG and oxaliplatin-GG adducts...
  12. doi request reprint Toxicity, DNA binding, and cell proliferation in male F344 rats following short-term gavage exposures to trans-2-hexenal
    Matthew D Stout
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina, USA
    Toxicol Pathol 36:232-46. 2008
    ..These findings suggest that the toxicity of hexenal was limited to the site of contact (stomach) and that the observed DNA adduct formation and cell proliferation occurred in the setting of severe tissue damage...
  13. ncbi request reprint The importance of 3,4-epoxy-1,2-butanediol and hydroxymethylvinyl ketone in 3-butene-1,2-diol associated mutagenicity
    Mark W Powley
    Environmental Sciences and Engineering, University of North Carolina School of Public Health, Chapel Hill, NC 27599 7431, USA
    Chem Biol Interact 166:182-90. 2007
    ....
  14. pmc Exposure-response of 1,2:3,4-diepoxybutane-specific N-terminal valine adducts in mice and rats after inhalation exposure to 1,3-butadiene
    Nadia I Georgieva
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 115:322-9. 2010
    ..This research supports the hypothesis that the lower susceptibility of rats to BD-induced carcinogenesis results from greatly reduced formation of DEB following exposure to BD...
  15. ncbi request reprint WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase
    Courtney G Woods
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Toxicol Sci 98:366-74. 2007
    ..These results provide new insight for understanding the mode of hepatocarcinogenic action of peroxisome proliferators...
  16. ncbi request reprint Molecular dosimetry and repair of N(2),3-ethenoguanine in rats exposed to vinyl chloride
    Eric J Morinello
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Cancer Res 62:5189-95. 2002
    ..These data are well suited for consideration in future risk assessments of VC that incorporate nontumor mode of action data...
  17. ncbi request reprint LC/MS/MS method for the quantitation of trans-2-hexenal-derived exocyclic 1,N(2)-propanodeoxyguanosine in DNA
    Matthew D Stout
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Chem Res Toxicol 19:563-70. 2006
    ..035 or 0.35 fmol/microg DNA. These data indicate that Hex-PdG is formed following hexenal treatment and that this method is suitable for in vitro or in vivo assessment of Hex-PdG formation...
  18. pmc Structural characterization of formaldehyde-induced cross-links between amino acids and deoxynucleosides and their oligomers
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 132:3388-99. 2010
    ....
  19. ncbi request reprint Analysis of M1G-dR in DNA by aldehyde reactive probe labeling and liquid chromatography tandem mass spectrometry
    Yo Chan Jeong
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, North Carolina 27599 7431, USA
    Chem Res Toxicol 18:51-60. 2005
    ..By this assay, two M1G adducts/10(8) guanines can readily be detected. Furthermore, this approach should be applicable to the analysis of other aldehydic DNA adducts as well as the measurement of an array of DNA lesions...
  20. pmc Flanking bases influence the nature of DNA distortion by platinum 1,2-intrastrand (GG) cross-links
    Debadeep Bhattacharyya
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e23582. 2011
    ..OX) play an important role in the conformation and the conformational dynamics of Pt-DNA adducts, possibly explaining their influence on the ability of many damage-recognition proteins to bind to Pt-DNA adducts...
  21. pmc Development and application of an LC-MS/MS method for the detection of the vinyl chloride-induced DNA adduct N(2),3-ethenoguanine in tissues of adult and weanling rats following exposure to [(13)C(2)]-VC
    Esra Mutlu
    Department of Environmental Sciences and Engineering, and Curriculum in Toxicology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 23:1485-91. 2010
    ..The half-life of εG was found to be 150 days in the liver and lung and 75 days in the kidney, suggesting little or no active repair of this promutagenic adduct...
  22. pmc 1,3-Butadiene: Biomarkers and application to risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27559, USA
    Chem Biol Interact 192:150-4. 2011
    ..These data provide a scientific basis for improved risk assessment of BD...
  23. pmc Biomarkers of exposure and effect in human lymphoblastoid TK6 cells following [13C2]-acetaldehyde exposure
    Benjamin C Moeller
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 133:1-12. 2013
    ..These data can replace default assumptions of linear extrapolation to very low doses of exogenous acetaldehyde for risk prediction...
  24. pmc Phenotypic anchoring of acetaminophen-induced oxidative stress with gene expression profiles in rat liver
    Christine L Powell
    Curriculum in Toxicology and Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 93:213-22. 2006
    ....
  25. doi request reprint Formaldehyde-induced histone modifications in vitro
    Kun Lu
    Curriculum in Applied Science and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 21:1586-93. 2008
    ..Additionally, we found that formaldehyde-induced Schiff bases on lysine residues could inhibit the formation of PTM on histone, raising the possibility that formaldehyde might alter epigenetic regulation...
  26. pmc Pyrimido[1,2-a]-purin-10(3H)-one, M1G, is less prone to artifact than base oxidation
    Yo Chan Jeong
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, NC 27599 7431, USA
    Nucleic Acids Res 33:6426-34. 2005
    ..In addition, this study confirmed that the background number of M1G adducts reported in genomic DNA could have been overestimated by one to three orders of magnitude in previous reports...
  27. pmc Determination of N2-hydroxymethyl-dG adducts in the nasal epithelium and bone marrow of nonhuman primates following 13CD2-formaldehyde inhalation exposure
    Benjamin C Moeller
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, North Carolina 27599 7431, United States
    Chem Res Toxicol 24:162-4. 2011
    ..In contrast, only endogenous adducts were detectable in the bone marrow, even though ∼10 times more DNA was analyzed...
  28. pmc Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 25:664-75. 2012
    ..The use of highly sensitive LC-MS/MS and isotope labeled compounds for exposure solves these challenges and provides mechanistic insights on the formation and role of these DNA adducts...
  29. ncbi request reprint N-terminal globin adducts as biomarkers for formation of butadiene derived epoxides
    Gunnar Boysen
    Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Chem Biol Interact 166:84-92. 2007
    ..The analyses of BD derived protein adducts correlate well with the observed species and gender differences in BD-carcinogenesis and suggest that DEB may indeed be the most important metabolite...
  30. pmc Quantitative analysis of N-terminal valine peptide adducts specific for 1,2-epoxy-3-butene
    Nadia I Georgieva
    Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, NC, USA
    Chem Biol Interact 166:219-25. 2007
    ..Based on our experience with pyr-Val adduct assay we anticipate that this assay will be suitable for evaluation of HB-Val in multiple species...
  31. pmc Formaldehyde and epigenetic alterations: microRNA changes in the nasal epithelium of nonhuman primates
    Julia E Rager
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Environ Health Perspect 121:339-44. 2013
    ..Our previous investigation demonstrated that formaldehyde exposure in human lung cells caused disruptions in miRNA expression profiles in vitro...
  32. ncbi request reprint Low utilization of circulating glucose after food withdrawal in Snell dwarf mice
    Natasha L Brooks
    Department of Nutrition, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 282:35069-77. 2007
    ....
  33. pmc Detection of PIGO-deficient cells using proaerolysin: a valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system
    Jun Nakamura
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e33563. 2012
    ..This novel assay for DT40 cells provides a valuable tool to investigate the mode of action of mutations caused by reactive agents using a series of isogenic mutant DT40 cells...
  34. pmc Development of an ultraperformance liquid chromatography/mass spectrometry method to quantify cisplatin 1,2 intrastrand guanine-guanine adducts
    Irene M Baskerville-Abraham
    Curriculum in Toxicology, Center of Environmental Health and Susceptibility, and Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 22:905-12. 2009
    ..This will support the refinement of therapeutic regimes and appropriate individualized treatment protocols...
  35. ncbi request reprint Aldehydic DNA lesions in calf thymus DNA and HeLa S3 cells produced by bacterial quinone metabolites of fluoranthene and pyrene
    Joanna Zielinska-Park
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Carcinogenesis 25:1727-33. 2004
    ..This study exemplifies the importance of considering not only primary environmental pollutants, but also their biologically or chemically generated transformation products...
  36. ncbi request reprint Synthesis, characterization, and identification of N7-guanine adducts of isoprene monoepoxides in vitro
    Petra Begemann
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7431, USA
    Chem Res Toxicol 17:929-36. 2004
    ..Characterization of the N7-Gua adducts identified in incubations of DNA with IP monoepoxides represents the first step toward establishing biomarkers of IP metabolism and exposure...
  37. pmc Liquid chromatography electrospray ionization tandem mass spectrometry analysis method for simultaneous detection of trichloroacetic acid, dichloroacetic acid, S-(1,2-dichlorovinyl)glutathione and S-(1,2-dichlorovinyl)-L-cysteine
    Sungkyoon Kim
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, CB 7431, Chapel Hill, NC 27599, USA
    Toxicology 262:230-8. 2009
    ..037+/-0.015 nmol/ml (0.001 nmol/ml); DCVC, 0.0024+/-0.0009 nmol/ml (0.001 nmol/ml). This method opens new opportunities to increase throughput and decrease number of animals required for mechanistic studies on TCE in rodents...
  38. pmc Convenient, multi-well plate-based DNA damage response analysis using DT40 mutants is applicable to a high-throughput genotoxicity assay with characterization of modes of action
    John R Ridpath
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7431, USA
    Environ Mol Mutagen 52:153-60. 2011
    ....
  39. pmc Bis-butanediol-mercapturic acid (bis-BDMA) as a urinary biomarker of metabolic activation of butadiene to its ultimate carcinogenic species
    Srikanth Kotapati
    Department of Medicinal Chemistry and the Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA, Sealy Center for Environmental Health and Medicine SCEHM and the Department of Preventive Medicine and Community Health, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA, Department of Pathology, University of Vermont, Burlington, VT 05405, USA and Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Carcinogenesis 35:1371-8. 2014
    ..In humans, DHBMA was the most abundant BD-mercapturic acid excreted (93%), followed by THBMA (5%) and MHBMA (2%), whereas no bis-BDMA was detected. These results reveal significant differences in metabolism of BD between rats and humans...
  40. ncbi request reprint Effects of propylene oxide exposure on rat nasal respiratory cell proliferation
    Melva N Ríos-Blanco
    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 75:279-88. 2003
    ..These studies demonstrate a clear concordance between the site and exposure concentration for tumor induction and those causing significant increases in cell proliferation in the rat nose...
  41. pmc Formation of hydroxymethyl DNA adducts in rats orally exposed to stable isotope labeled methanol
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 126:28-38. 2012
    ....
  42. pmc Distribution of DNA adducts caused by inhaled formaldehyde is consistent with induction of nasal carcinoma but not leukemia
    Kun Lu
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 116:441-51. 2010
    ....
  43. ncbi request reprint Cells deficient in the FANC/BRCA pathway are hypersensitive to plasma levels of formaldehyde
    John R Ridpath
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Cancer Res 67:11117-22. 2007
    ....
  44. pmc Formation of S-[1-(N2-deoxyguanosinyl)methyl]glutathione between glutathione and DNA induced by formaldehyde
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 131:3414-5. 2009
    ..This adduct is unique because of the involvement of S-hydroxymethylglutathione which is a key player during the detoxication of formaldehyde...
  45. pmc Cells deficient in PARP-1 show an accelerated accumulation of DNA single strand breaks, but not AP sites, over the PARP-1-proficient cells exposed to MMS
    Brian F Pachkowski
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC, USA
    Mutat Res 671:93-9. 2009
    ..These data suggest that during exposure, PARP-1 impacts the stage of BER after excision of the deoxyribosephosphate moiety from the 5' end of DNA strand breaks by polymerase beta...
  46. ncbi request reprint Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage
    Christine L Powell
    Curriculum in Toxicology and Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA
    Cancer Lett 229:1-11. 2005
    ..Here, we demonstrate the utility of measuring changes in expression of BER genes as a sensitive in vivo biomarker for oxidative DNA damage...
  47. pmc Accurate quantitation of standard peptides used for quantitative proteomics
    Narisa K Bordeerat
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, USA
    Proteomics 9:3939-44. 2009
    ..1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required...
  48. ncbi request reprint Profiling of ecdysteroids in complex biological samples using liquid chromatography/ion trap mass spectrometry
    Yutai Li
    Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina at Chapel Hill, NC 27599, USA
    Rapid Commun Mass Spectrom 20:185-92. 2006
    ....
  49. ncbi request reprint New immunoaffinity-LC-MS/MS methodology reveals that Aag null mice are deficient in their ability to clear 1,N6-etheno-deoxyadenosine DNA lesions from lung and liver in vivo
    Amy Joan L Ham
    Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill 27599, USA
    DNA Repair (Amst) 3:257-65. 2004
    ..Our results demonstrate that while Aag clearly represents the major DNA repair enzyme for the in vivo removal (epsilon)dA bases, these lesions can also be eliminated from the genome via an alternative mechanism...
  50. ncbi request reprint 1,N2-propanodeoxyguanosine adducts of the 1,3-butadiene metabolite, hydroxymethylvinyl ketone
    Mark W Powley
    Department of Pathology and Laboratory Medicine, University of North Carolina Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599 7400, USA
    Chem Res Toxicol 16:1448-54. 2003
    ..Overall, our research demonstrates that HMVK can form promutagenic DNA adducts and it therefore has the potential to play a role in BD-associated mutagenicity...
  51. ncbi request reprint Formation of M1G-dR from endogenous and exogenous ROS-inducing chemicals
    Yo Chan Jeong
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Free Radic Biol Med 39:1021-9. 2005
    ....
  52. ncbi request reprint Synthesis of chlorinated and non-chlorinated biphenyl-2,3- and 3,4-catechols and their [2H3]-isotopomers
    Po Hsiung Lin
    Department of Environmental Sciences and Engineering, University of North Carolina, CB7431, Chapel Hill, North Carolina 27599 7431, USA
    Org Biomol Chem 2:2624-9. 2004
    ..This hydrogenation procedure provides a simple and convenient approach to selective reduction of aryl nitro groups in the presence of halogen ring substituents...
  53. pmc Accumulation of M1dG DNA adducts after chronic exposure to PCBs, but not from acute exposure to polychlorinated aromatic hydrocarbons
    Yo Chan Jeong
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, NC 27599, USA
    Free Radic Biol Med 45:585-91. 2008
    ..In summary, the results from the present study support the hypothesis that oxidative DNA damage plays a key role in toxicity and carcinogenicity following long-term PCB exposure...
  54. pmc Neuroendocrine inhibition of glucose production and resistance to cancer in dwarf mice
    J McKee Alderman
    University of North Carolina, Chapel Hill, NC 27599, USA
    Exp Gerontol 44:26-33. 2009
    ..In summary, our results in Snell dwarf mice suggest that the pituitary gland and adipose tissue are part of a neuroendocrine loop that lowers the risk of cancer during aging by reducing the availability of glucose...
  55. ncbi request reprint Oxidative DNA damage from potassium bromate exposure in Long-Evans rats is not enhanced by a mixture of drinking water disinfection by-products
    Kevin S McDorman
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA
    Chem Biol Interact 152:107-17. 2005
    ..These data are consistent with previous studies where chronic exposure to this chemical mixture in drinking water resulted in a less than additive carcinogenic response in Tsc2 mutant Long-Evans rats...
  56. ncbi request reprint Synthesis and characterization of peptides containing a cyclic Val adduct of diepoxybutane, a possible biomarker of human exposure to butadiene
    K Jayaraj
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, 27599 7431, USA
    Chem Res Toxicol 16:637-43. 2003
    ..Use of FMOC-protected (2)H(3)-Leu in the automated oligopeptide synthesis provided the required isotopomers for use as internal standard...
  57. pmc Measurement of Endogenous versus Exogenous Formaldehyde-Induced DNA-Protein Crosslinks in Animal Tissues by Stable Isotope Labeling and Ultrasensitive Mass Spectrometry
    Yongquan Lai
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
    Cancer Res 76:2652-61. 2016
    ..Cancer Res; 76(9); 2652-61. ©2016 AACR. ..
  58. pmc Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations
    Vyom Sharma
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, Chapel Hill, NC 27599, USA
    Oncotarget 7:25377-90. 2016
    ....
  59. pmc Formation, Accumulation, and Hydrolysis of Endogenous and Exogenous Formaldehyde-Induced DNA Damage
    Rui Yu
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina, Chapel Hill, North Carolina 27599
    Toxicol Sci 146:170-82. 2015
    ....
  60. pmc Formaldehyde-associated changes in microRNAs: tissue and temporal specificity in the rat nose, white blood cells, and bone marrow
    Julia E Rager
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina, Chapel Hill, North Carolina 27599
    Toxicol Sci 138:36-46. 2014
    ..These data inform our current hypothesis that formaldehyde-induced inflammatory signals originating in the nose may drive WBC effects. ..
  61. pmc Molecular dosimetry of N2-hydroxymethyl-dG DNA adducts in rats exposed to formaldehyde
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 24:159-61. 2011
    ..In contrast, exogenous adducts were not detectable in the bone marrow of rats exposed to 15.2 ppm [(13)CD(2)] formaldehyde...
  62. ncbi request reprint Expression of base excision DNA repair genes is a sensitive biomarker for in vivo detection of chemical-induced chronic oxidative stress: identification of the molecular source of radicals responsible for DNA damage by peroxisome proliferators
    Ivan Rusyn
    Laboratory of Environmental Genomics, Department of Environmental Sciences and Engineering, University of North Carolina School of Public Health, Chapel Hill, North Carolina 27599, USA
    Cancer Res 64:1050-7. 2004
    ....
  63. pmc Abasic sites preferentially form at regions undergoing DNA replication
    Paul D Chastain
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7525, USA
    FASEB J 24:3674-80. 2010
    ..They also reveal that there is increased susceptibility to oxidative damage in DNA regions undergoing replication, which may explain the previously observed clustering of AP sites...
  64. ncbi request reprint XRCC1 genotype and breast cancer: functional studies and epidemiologic data show interactions between XRCC1 codon 280 His and smoking
    Brian F Pachkowski
    Department of Environmental Sciences, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Cancer Res 66:2860-8. 2006
    ..0) and 399Arg (D' = 1.0). These data suggest that less common, functional polymorphisms may lie within common haplotypes and drive gene-environment interactions...
  65. ncbi request reprint NMR solution structure of an oxaliplatin 1,2-d(GG) intrastrand cross-link in a DNA dodecamer duplex
    Yibing Wu
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    J Mol Biol 341:1251-69. 2004
    ..These structural differences may explain some of the biological differences between oxaliplatin- and cisplatin-GG adducts...
  66. ncbi request reprint Differential induction of N(2),3-ethenoguanine in rat brain and liver after exposure to vinyl chloride
    Eric J Morinello
    Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Cancer Res 62:5183-8. 2002
    ..Future exposures of weanling rats to the stable isotopically labeled compound will be necessary to conclusively determine whether this increase was due to VC...
  67. ncbi request reprint Future directions in butadiene risk assessment and the role of cross-species internal dosimetry
    James A Swenberg
    Laboratory of Molecular Mutagenesis and Carcinogenesis, University of North Carolina, Chapel Hill, NC 27599, USA
    Chem Biol Interact 166:78-83. 2007
    ..Further improvements in analytical sensitivity for the pyr-Val adduct are on the horizon. Epidemiology studies are also described and ongoing efforts promise to help bridge our understanding of past and future risks...
  68. pmc Adiponectin lowers glucose production by increasing SOGA
    Rachael B Cowherd
    Departments of Nutrition, School of Medicine and Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Am J Pathol 177:1936-45. 2010
    ..Circulating SOGA increased in parallel with adiponectin and insulin activity in both humans and mice. These results suggest that adiponectin-mediated increases in SOGA contribute to the inhibition of glucose production...
  69. ncbi request reprint Quantitation of choline and its metabolites in tissues and foods by liquid chromatography/electrospray ionization-isotope dilution mass spectrometry
    Hasan Koc
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599, USA
    Anal Chem 74:4734-40. 2002
    ..The new LC/ESI-IDMS method was validated using the existing published GC/IDMS method...
  70. pmc Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time
    Jun Nakamura
    Department of Environmental Sciences and Engineering, Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 31:e104. 2003
    ..These results indicate that the analysis of intracellular NAD(P)H level using water-soluble tetrazolium salt can assess an imbalance of SSB repair in living cells in real time...
  71. ncbi request reprint Exposure-dependent accumulation of N-(2-hydroxypropyl)valine in hemoglobin of F344 rats exposed to propylene oxide by the inhalation route
    Melva N Ríos-Blanco
    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7400, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 778:383-91. 2002
    ..When the present rat data were compared with recent N-HPVal measurements in humans, similar binding was found...
  72. pmc Micromolar concentrations of hydrogen peroxide induce oxidative DNA lesions more efficiently than millimolar concentrations in mammalian cells
    Jun Nakamura
    Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 31:1790-5. 2003
    ..These results suggest that ADLs induced by submillimolar levels of H2O2 may be due to a Fenton-type reaction between H2O2 and intracellular iron ions in mammalian cells...
  73. pmc A mass spectrometric method to simultaneously measure a biomarker and dilution marker in exhaled breath condensate
    Charles R Esther
    Division of Pediatric Pulmonology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27517, USA
    Rapid Commun Mass Spectrom 22:701-5. 2008
    ..These results demonstrate that mass spectrometry can be used successfully in EBC analysis to simultaneously detect a biomarker for airway inflammation and control for variable dilution...
  74. ncbi request reprint Analysis of DNA adducts in rats exposed to pentachlorophenol
    Po Hsiung Lin
    Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, NC 27599 7400, USA
    Carcinogenesis 23:365-9. 2002
    ....
  75. ncbi request reprint Identification of covalent modifications in P450 2E1 by 1,2-epoxy-3-butene in vitro
    Gunnar Boysen
    Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Chem Biol Interact 166:170-5. 2007
    ..The data support the hypothesis that EB can inactivate P450 2E1 by covalent modifications and thus add an additional regulatory mechanism for BD metabolism...
  76. ncbi request reprint Applications of mass spectrometry for quantitation of DNA adducts
    Hasan Koc
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 778:323-43. 2002
    ....
  77. ncbi request reprint Anti-AIDS agents 65: investigation of the in vitro oxidative metabolism of 3',4'-Di-O-(-)-camphanoyl-(+)-cis-khellactone derivatives as potent anti-hiv agents
    Madoka Suzuki
    Division of Medicinal Chemistry, School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599 7360, USA
    Drug Metab Dispos 33:1588-92. 2005
    ....
  78. ncbi request reprint Epifluorescence microscopy and image analysis of high-level polycyclic aromatic hydrocarbon contamination in soils
    J Chadwick Roper
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7431, USA
    Environ Toxicol Chem 25:3093-100. 2006
    ..These qualitative descriptions of the microscale spatial distribution of PAH contamination are presented as potential endpoints for future research on biogeochemical interactions in heavily contaminated solids...
  79. pmc Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress
    Robert W Sobol
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 99:6860-5. 2002
    ..These results illustrate that BER plays a role in protecting mouse embryonic fibroblast cells against methylation-induced mutations and characterize the effect of a particular combination of BER gene defect and environmental exposure...
  80. ncbi request reprint Age-, gender-, and species-dependent mutagenicity in T cells of mice and rats exposed by inhalation to 1,3-butadiene
    Quanxin Meng
    Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr SE, Albuquerque, NM 87108, USA
    Chem Biol Interact 166:121-31. 2007
    ..03). These results help explain part of the differing outcomes/interpretations of data in earlier Hprt mutation studies in BD-exposed rodents...
  81. ncbi request reprint Influence of metabolic genotypes on biomarkers of exposure to 1,3-butadiene in humans
    Silvia Fustinoni
    Dipartimento di Medicina del Lavoro, Istituti Clinici di Perfezionamento, Milano, Italy 20122
    Cancer Epidemiol Biomarkers Prev 11:1082-90. 2002
    ..698). Our results indicate that the THBVal level is influenced by genotypes, and that the analysis of combined polymorphisms may be the key to a better understanding of the role played by polymorphism of BD-metabolizing enzymes...
  82. ncbi request reprint Hypersensitivity of DNA polymerase beta null mouse fibroblasts reflects accumulation of cytotoxic repair intermediates from site-specific alkyl DNA lesions
    Julie K Horton
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    DNA Repair (Amst) 2:27-48. 2003
    ....
  83. ncbi request reprint Evaluation of effects from repeated inhalation exposure of F344 rats to high concentrations of propylene
    Lynn H Pottenger
    Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, MI 48674, USA
    Toxicol Sci 97:336-47. 2007
    ..In addition, these data indicate that formation of N7-HPGua does not correlate with any measure of genotoxic effect, neither mutagenic nor clastogenic...
  84. doi request reprint Possible genotoxic modes of action for naphthalene
    David Brusick
    Department of Environmental Engineering, Carnegie Mellon University, USA
    Regul Toxicol Pharmacol 51:S43-50. 2008
    ..The report also outlines a set of six studies that could resolve some of these key uncertainties...
  85. ncbi request reprint Aldehydic DNA lesions induced by catechol estrogens in calf thymus DNA
    Po Hsiung Lin
    Department of Environmental Engineering, National Chung Hsing University, Taichung 402, Taiwan
    Carcinogenesis 24:1133-41. 2003
    ..We hypothesize that in addition to DNA adducts and oxidized bases, the ADL induced by estrogen quinonoid-mediated oxidative stress may play a role in estrogen-induced carcinogenicity...
  86. ncbi request reprint Molecular epidemiological studies in 1,3-butadiene exposed Czech workers: female-male comparisons
    Richard J Albertini
    BioMosaics, Inc, 665 Spear Street, Burlington, VT 05401, USA
    Chem Biol Interact 166:63-77. 2007
    ..However, as in the first, differences in the metabolic detoxification of BD as reflected in relative amounts of the M1 and M2 urinary metabolites were associated with genotypes, this time both GST and EH...
  87. ncbi request reprint Induction of cytotoxicity, aldehydic DNA lesions, and poly(ADP-ribose) polymerase-1 activation by catechol derivatives of pentachlorophenol in calf thymus DNA and in human breast cancer cells
    Chia Hua Lin
    Department of Environmental Engineering, National Chung Hsing University, Taichung 402, Taiwan
    Chem Res Toxicol 18:257-64. 2005
    ..Overall, these findings indicate that dechlorination may decrease the potentials of chlorinated catechols to induce oxidative DNA lesions and cytotoxic effects in living cells...
  88. ncbi request reprint Analysis of 7-methylguanine using isotope dilution and gas chromatography/electron-capture negative chemical ionization mass spectrometry
    Su Yin Chiang
    Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan
    Rapid Commun Mass Spectrom 19:1915-20. 2005
    ..The results indicate that this method could be applied to the analysis of endogenous and exogenous 7-MG in human tissues for future molecular epidemiology studies on potential health effects caused by methylating agents...
  89. ncbi request reprint Propylene oxide in blood and soluble nonprotein thiols in nasal mucosa and other tissues of male Fischer 344/N rats exposed to propylene oxide vapors--relevance of glutathione depletion for propylene oxide-induced rat nasal tumors
    Moung S Lee
    Institute of Toxicology, GSF National Research Center for Environment and Health, D 85764 Neuherberg, Germany
    Toxicol Sci 83:177-89. 2005
    ..We conclude that continuous, severe perturbation of GSH in RNM following repeated high PO exposures may lead to inflammatory lesions and cell proliferation, critical steps on the path towards tumorigenicity...
  90. ncbi request reprint DNA damage in nasal and brain tissues of canines exposed to air pollutants is associated with evidence of chronic brain inflammation and neurodegeneration
    LILIAN CALDERON-GARCIDUENAS
    Environmental Pathology Program, University of North Carolina at Chapel Hill, North Carolina 27599 7310, USA
    Toxicol Pathol 31:524-38. 2003
    ..These data suggest that Alzheimer's disease may be the sequela of air pollutant exposures and the resulting systemic inflammation...
  91. ncbi request reprint Endogenous deoxyribonucleic Acid (DNA) damage in human tissues: a comparison of ethenobases with aldehydic DNA lesions
    Alain Barbin
    International Agency for Research on Cancer, Lyon, France
    Cancer Epidemiol Biomarkers Prev 12:1241-7. 2003
    ..These data are in line with previous studies showing that brain tissues are more susceptible to oxidative stress and lipid peroxidation than other tissues...
  92. ncbi request reprint Associations of antioxidant nutrients and oxidative DNA damage in healthy African-American and White adults
    Joanne L Watters
    Department of Nutrition, Division of Digestive Diseases and Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina USA
    Cancer Epidemiol Biomarkers Prev 16:1428-36. 2007
    ..21; P = 0.02) and in African-American men (r = 0.63; P = 0.01) after adjusting for covariates. This study is among the first to examine these associations in a sample of healthy adults with an adequate representation of African-Americans...
  93. ncbi request reprint Comparison of analyses of urinary 8-hydroxy-2'-deoxyguanosine by isotope-dilution liquid chromatography with electrospray tandem mass spectrometry and by enzyme-linked immunosorbent assay
    Chiung Wen Hu
    Division of Environmental Health and Occupational Medicine, National Health Research Institute, Kaohsiung, Taiwan
    Rapid Commun Mass Spectrom 18:505-10. 2004
    ....
  94. ncbi request reprint Dose-dependent transitions in mechanisms of toxicity: case studies
    William Slikker
    US FDA National Center for Toxicological Research, Jefferson, AR 72079, USA
    Toxicol Appl Pharmacol 201:226-94. 2004
    ..The case studies formed the basis for technical discourse at two scientific workshops in 2003...
  95. ncbi request reprint Dose-dependent transitions in mechanisms of toxicity
    William Slikker
    US FDA National Center for Toxicological Research, Jefferson, AR 72079, USA
    Toxicol Appl Pharmacol 201:203-25. 2004
    ..This paper addresses the issues discussed at both workshops, and presents the consensus conclusions drawn by expert participants...
  96. ncbi request reprint Quantification of 2'-fluoro-2'-deoxyuridine and 2'-fluoro-2'-deoxycytidine in DNA and RNA isolated from rats and woodchucks using LC/MS/MS
    Frank C Richardson
    OSI Pharmaceuticals, Inc, Boulder, Colorado 80301, USA
    Chem Res Toxicol 15:922-6. 2002
    ..These data demonstrate that 2'-fluoropyrimidines are incorporated into DNA and RNA of various tissues of rats and woodchucks following long-term administration...