Donald Siegel

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc Validation of glypican-3-specific scFv isolated from paired display/secretory yeast display library
    Yonghai Li
    Medicine and Research Services, Philadelphia VA Medical Center, PA 19104, USA
    BMC Biotechnol 12:23. 2012
  2. doi Translational applications of antibody phage display
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 510 Stellar Chance Laboratories, 422 Curie Blvd, Philadelphia, PA, 19104, USA
    Immunol Res 42:118-31. 2008
  3. ncbi Phage display-based molecular methods in immunohematology
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Transfusion 47:89S-94S. 2007
  4. ncbi Phage display tools for blood typing
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Curr Hematol Rep 4:459-64. 2005
  5. ncbi Developing phage display tools for use in transfusion medicine
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Transfusion 45:100S-108S. 2005
  6. ncbi Isolation of cell surface-specific human monoclonal antibodies using phage display and magnetically-activated cell sorting: applications in immunohematology
    D L Siegel
    Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Immunol Methods 206:73-85. 1997
  7. ncbi The human immune response to red blood cell antigens as revealed by repertoire cloning
    D L Siegel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, USA
    Immunol Res 17:239-51. 1998
  8. ncbi Section 5: Structural/genetic analysis of mAbs to blood group antigens. Coordinator's report
    D L Siegel
    University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Transfus Clin Biol 9:83-97. 2002
  9. ncbi Recombinant monoclonal antibody technology
    D L Siegel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Room 510 Stellar Chance Building, 422 Curie Blvd, Philadelphia, PA 19104, USA
    Transfus Clin Biol 9:15-22. 2002
  10. pmc Isolation of pathogenic monoclonal anti-desmoglein 1 human antibodies by phage display of pemphigus foliaceus autoantibodies
    Ken Ishii
    Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Invest Dermatol 128:939-48. 2008

Research Grants

Detail Information

Publications19

  1. pmc Validation of glypican-3-specific scFv isolated from paired display/secretory yeast display library
    Yonghai Li
    Medicine and Research Services, Philadelphia VA Medical Center, PA 19104, USA
    BMC Biotechnol 12:23. 2012
    ..We hypothesized that we could utilize a recently described paired display/secretory yeast library to isolate human-derived scFv against glypican-3 for potential diagnostic and/or therapeutic application...
  2. doi Translational applications of antibody phage display
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 510 Stellar Chance Laboratories, 422 Curie Blvd, Philadelphia, PA, 19104, USA
    Immunol Res 42:118-31. 2008
    ....
  3. ncbi Phage display-based molecular methods in immunohematology
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Transfusion 47:89S-94S. 2007
  4. ncbi Phage display tools for blood typing
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Curr Hematol Rep 4:459-64. 2005
    ....
  5. ncbi Developing phage display tools for use in transfusion medicine
    Don L Siegel
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Transfusion 45:100S-108S. 2005
  6. ncbi Isolation of cell surface-specific human monoclonal antibodies using phage display and magnetically-activated cell sorting: applications in immunohematology
    D L Siegel
    Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Immunol Methods 206:73-85. 1997
    ....
  7. ncbi The human immune response to red blood cell antigens as revealed by repertoire cloning
    D L Siegel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, USA
    Immunol Res 17:239-51. 1998
    ..Our results have provided a comprehensive genetic and serologic analysis of anit-Rh(D) antibodies within single alloimmunized individuals thereby offering new insights into the development of human immune repertoires...
  8. ncbi Section 5: Structural/genetic analysis of mAbs to blood group antigens. Coordinator's report
    D L Siegel
    University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Transfus Clin Biol 9:83-97. 2002
    ..Analysis of other mAbs demonstrated the value of antibody sequence data for the design and production of novel reagents useful in blood group serology...
  9. ncbi Recombinant monoclonal antibody technology
    D L Siegel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Room 510 Stellar Chance Building, 422 Curie Blvd, Philadelphia, PA 19104, USA
    Transfus Clin Biol 9:15-22. 2002
    ..The use of such recombinant methods in transfusion medicine is anticipated to play an important role in the development and production of renewable supplies of low-cost reagents for diagnostic and therapeutic applications...
  10. pmc Isolation of pathogenic monoclonal anti-desmoglein 1 human antibodies by phage display of pemphigus foliaceus autoantibodies
    Ken Ishii
    Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Invest Dermatol 128:939-48. 2008
    ..These mAbs will be useful for studying Dsg1 function and mechanisms of blister formation in PF and for developing targeted therapies and tools to monitor disease activity...
  11. ncbi Genetic analysis of autoantibodies in idiopathic thrombocytopenic purpura reveals evidence of clonal expansion and somatic mutation
    Jessica H Roark
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Blood 100:1388-98. 2002
    ..The extraordinarily high usage of the V(H)3-30 heavy-chain gene in these patients has implications for the pathogenesis, diagnosis, and management of chronic ITP...
  12. ncbi Targeting pemphigus autoantibodies through their heavy-chain variable region genes
    Aimee S Payne
    Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Invest Dermatol 127:1681-91. 2007
    ..Together, these data suggest novel V(H) gene-targeted approaches toward PV treatment...
  13. pmc Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display
    Aimee S Payne
    Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 115:888-99. 2005
    ..Detailed characterization of these pemphigus mAbs should lead to a better understanding of the immunopathogenesis of disease and to more specifically targeted therapeutic approaches...
  14. ncbi Mechanisms of action of therapeutics in idiopathic thrombocytopenic purpura
    Douglas B Cines
    University of Pennsylvania School of Medicine, Department of Pathology and Laboratory Medicine, 513A Stellar Chance, 422 Curie Boulevard, Philadelphia, PA 19104, USA
    J Pediatr Hematol Oncol 25:S52-6. 2003
    ..Rationale therapy awaits a more thorough understanding of autoantibody production...
  15. pmc Update on the cloning of monoclonal anti-desmoglein antibodies from human pemphigus patients: implications for targeted therapy
    John R Stanley
    University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Vet Dermatol 20:327-30. 2009
    ..In summary, the antibody response is restricted and, therefore, it may be feasible to target the specific pathogenic antibodies for therapy...
  16. ncbi Post-transplant outcomes of induction therapy for myeloma: thalidomide and dexamethasone versus doxorubicin, vincristine, and dexamethasone prior to high-dose melphalan with autologous stem cell support
    Dan T Vogl
    Bone Marrow and Stem Cell Transplant Program, Abramson Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Am J Hematol 82:1071-5. 2007
    ..Future trials comparing induction therapies should examine progression-free and overall survival after transplant to confirm this benefit...
  17. pmc Antibodies to the desmoglein 1 precursor proprotein but not to the mature cell surface protein cloned from individuals without pemphigus
    Jun Yamagami
    Department of Dermatology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Immunol 183:5615-21. 2009
    ..However, presentation of peptides from Dsg1 by preDsg1-specific B cells may be one step in developing autoimmunity in PF...
  18. pmc Homologous regions of autoantibody heavy chain complementarity-determining region 3 (H-CDR3) in patients with pemphigus cause pathogenicity
    Jun Yamagami
    Department of Dermatology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 120:4111-7. 2010
    ..These studies indicate that H-CDR3 is critical for pathogenicity of a human autoantibody, that a small region (even 1 amino acid) can mediate pathogenicity, and that pathogenicity can be uncoupled from binding in these antibodies...
  19. ncbi Selecting antibodies to cell-surface antigens using magnetic sorting techniques
    Don L Siegel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA
    Methods Mol Biol 178:219-26. 2002

Research Grants2

  1. Phage Display Tools for Automated Blood Typing
    Donald Siegel; Fiscal Year: 2007
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  2. MONOCLONAL ITP AUTOANTIBODIES--PARADIGM FOR AUTOIMMUNITY
    Donald Siegel; Fiscal Year: 2002
    ..Ultimately, the proposed approach may be applied to the understanding of other autoimmune disorders. ..