David A Rasko

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. pmc The QseC adrenergic signaling cascade in Enterohemorrhagic E. coli (EHEC)
    David T Hughes
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS Pathog 5:e1000553. 2009
  2. pmc Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli
    Susan R Steyert
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Institute for Genome Sciences Baltimore, MD, USA
    Front Cell Infect Microbiol 2:133. 2012
  3. pmc CadA negatively regulates Escherichia coli O157:H7 adherence and intestinal colonization
    Roberto C Vazquez-Juarez
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    Infect Immun 76:5072-81. 2008
  4. pmc Role of the K2 capsule in Escherichia coli urinary tract infection and serum resistance
    Eric L Buckles
    Department of Medicine, University of Maryland School of Medicine, MD, USA
    J Infect Dis 199:1689-97. 2009
  5. doi request reprint Genomic characterization of asymptomatic Escherichia coli isolated from the neobladder
    Jason W Sahl
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Microbiology 157:1088-102. 2011
  6. pmc The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis
    Nicola C Reading
    Department of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Proc Natl Acad Sci U S A 106:5889-94. 2009
  7. pmc Transcriptome of swarming Proteus mirabilis
    Melanie M Pearson
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Infect Immun 78:2834-45. 2010
  8. pmc Characterization of intracellular growth regulator icgR by utilizing transcriptomics to identify mediators of pathogenesis in Shigella flexneri
    Carolyn R Morris
    Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Infect Immun 81:3068-76. 2013
  9. pmc Genomic comparison of multi-drug resistant invasive and colonizing Acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity
    Jason W Sahl
    Department of Microbiology, Institute for Genome Sciences, Baltimore, MD 21201, USA
    BMC Genomics 12:291. 2011
  10. pmc Chemical sensing in mammalian host-bacterial commensal associations
    David T Hughes
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 107:9831-6. 2010

Collaborators

Detail Information

Publications41

  1. pmc The QseC adrenergic signaling cascade in Enterohemorrhagic E. coli (EHEC)
    David T Hughes
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS Pathog 5:e1000553. 2009
    ..Understanding adrenergic regulation of a bacterial cell is a powerful approach for studying the underlying mechanisms of stress and cellular survival...
  2. pmc Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli
    Susan R Steyert
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Institute for Genome Sciences Baltimore, MD, USA
    Front Cell Infect Microbiol 2:133. 2012
    ..A wide range of basal and induced toxin induction was observed. Overall, this is a first significant foray into the genome space of this unexplored group of emerging and divergent pathogens...
  3. pmc CadA negatively regulates Escherichia coli O157:H7 adherence and intestinal colonization
    Roberto C Vazquez-Juarez
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 1070, USA
    Infect Immun 76:5072-81. 2008
    ..In the infant rabbit model, the cadA mutant outcompeted the wild-type strain in the ileum but not in the cecum or mid-colon, raising the possibility that CadA negatively regulates EHEC pathogenicity in a tissue-specific fashion...
  4. pmc Role of the K2 capsule in Escherichia coli urinary tract infection and serum resistance
    Eric L Buckles
    Department of Medicine, University of Maryland School of Medicine, MD, USA
    J Infect Dis 199:1689-97. 2009
    ..Capsule expression may be important during ascending Escherichia coli urinary tract infections (UTIs)...
  5. doi request reprint Genomic characterization of asymptomatic Escherichia coli isolated from the neobladder
    Jason W Sahl
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Microbiology 157:1088-102. 2011
    ..coli, suggesting why they can colonize the transplanted intestinal tissue but rarely progress to acute pyelonephritis or more severe disease...
  6. pmc The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis
    Nicola C Reading
    Department of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Proc Natl Acad Sci U S A 106:5889-94. 2009
    ..QseG is an OM protein necessary for translocation of TTSS effectors that also works in conjunction with a 2-component signaling system that senses host stress signals...
  7. pmc Transcriptome of swarming Proteus mirabilis
    Melanie M Pearson
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA
    Infect Immun 78:2834-45. 2010
    ..These data suggest that the consolidation phase is a state in which P. mirabilis prepares for the next wave of swarming...
  8. pmc Characterization of intracellular growth regulator icgR by utilizing transcriptomics to identify mediators of pathogenesis in Shigella flexneri
    Carolyn R Morris
    Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Infect Immun 81:3068-76. 2013
    ....
  9. pmc Genomic comparison of multi-drug resistant invasive and colonizing Acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity
    Jason W Sahl
    Department of Microbiology, Institute for Genome Sciences, Baltimore, MD 21201, USA
    BMC Genomics 12:291. 2011
    ..baumannii strains isolated from distinct body sites. Isolates from blood, peri-anal, and wound sources were examined in an attempt to identify genetic features that could be correlated to each isolation source...
  10. pmc Chemical sensing in mammalian host-bacterial commensal associations
    David T Hughes
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 107:9831-6. 2010
    ....
  11. pmc Refining the pathovar paradigm via phylogenomics of the attaching and effacing Escherichia coli
    Tracy H Hazen
    Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 110:12810-5. 2013
    ..coli pathovars than to text modification AEEC. Our findings demonstrate evolutionary relationships among diverse AEEC pathogens and the utility of phylogenomics for lineage-specific identification of AEEC clinical isolates. ..
  12. pmc Targeting QseC signaling and virulence for antibiotic development
    David A Rasko
    Department of Microbiology, University of Texas UT Southwestern Medical Center, Dallas, TX 75390, USA
    Science 321:1078-80. 2008
    ..Inhibition of signaling offers a strategy for the development of broad-spectrum antimicrobial drugs...
  13. pmc Global effects of the cell-to-cell signaling molecules autoinducer-2, autoinducer-3, and epinephrine in a luxS mutant of enterohemorrhagic Escherichia coli
    Melissa M Kendall
    University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9048, USA
    Infect Immun 75:4875-84. 2007
    ..This study began to examine the global signaling networks in EHEC and revealed expression profiles that are signal and pathogen specific...
  14. pmc Hfq virulence regulation in enterohemorrhagic Escherichia coli O157:H7 strain 86-24
    Melissa M Kendall
    Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9048, USA
    J Bacteriol 193:6843-51. 2011
    ..Altogether, these data indicate that Hfq plays a regulatory role in EHEC 86-24 that is different from what has been reported for EHEC strain EDL933 and that the role of Hfq in EHEC virulence regulation extends beyond the LEE...
  15. pmc The LysR-type regulator QseA regulates both characterized and putative virulence genes in enterohaemorrhagic Escherichia coli O157:H7
    Melissa M Kendall
    University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    Mol Microbiol 76:1306-21. 2010
    ..Taken together, these results indicate that QseA acts as a global regulator in EHEC, co-ordinating expression of virulence genes...
  16. pmc Origins of the E. coli strain causing an outbreak of hemolytic-uremic syndrome in Germany
    David A Rasko
    University of Maryland School of Medicine, Institute for Genome Sciences and Department of Microbiology, Baltimore, USA
    N Engl J Med 365:709-17. 2011
    ..coli strains. Preliminary genetic characterization of the outbreak strain suggested that, unlike most of these strains, it should be classified within the enteroaggregative pathotype of E. coli...
  17. pmc Shigella flexneri effectors OspE1 and OspE2 mediate induced adherence to the colonic epithelium following bile salts exposure
    Christina S Faherty
    Department of Medicine, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, USA
    Mol Microbiol 85:107-21. 2012
    ..The adhesins required for Shigella attachment to the colonic epithelium may serve as ideal targets for vaccine development...
  18. pmc QseA and GrlR/GrlA regulation of the locus of enterocyte effacement genes in enterohemorrhagic Escherichia coli
    Regan M Russell
    University of Texas Southwestern Medical Center, Department of Microbiology, Dallas, TX 75390 9048, USA
    J Bacteriol 189:5387-92. 2007
    ..This work demonstrates that GrlR/GrlA activate LEE2 transcription in a Ler-independent fashion, whereas transcription of grlRA is activated by QseA in both Ler-dependent and -independent manners...
  19. doi request reprint Outbreak caused by cad-negative Shiga toxin-producing Escherichia coli O111, Oklahoma
    Veronica E Calderon
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 1070, USA
    Foodborne Pathog Dis 7:107-9. 2010
    ..coli, particularly Shiga toxin-producing E. coli O111 isolates, that have the potential of causing human infections and outbreaks...
  20. pmc Antimicrobial resistance-conferring plasmids with similarity to virulence plasmids from avian pathogenic Escherichia coli strains in Salmonella enterica serovar Kentucky isolates from poultry
    W Florian Fricke
    Institute for Genome Sciences, University of Maryland School of Medicine, 801 W Baltimore St, Baltimore, MD 21201, USA
    Appl Environ Microbiol 75:5963-71. 2009
    ....
  21. doi request reprint Novel approaches to bacterial infection therapy by interfering with cell-to-cell signaling
    David A Rasko
    University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
    Curr Protoc Microbiol . 2009
    ..We also provide a detailed protocol using a specific example of signaling inhibitor identification using enterohemorrhagic Escherichia coli as a model system...
  22. pmc Analysis of global transcriptional profiles of enterotoxigenic Escherichia coli isolate E24377A
    Jason W Sahl
    Department of Microbiology and Immunology and Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Infect Immun 80:1232-42. 2012
    ..These results provide transcriptional targets and putative mechanisms that should help improve understanding of the global regulatory networks and virulence expression in this important human pathogen...
  23. pmc Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli
    Amanda L Lloyd
    Department of Microbiology and Immunology, University of Michigan Medical School, 5641 Medical Science Bldg II, 1150 West Medical Center Dr, Ann Arbor, MI 48109 0620, USA
    J Bacteriol 189:3532-46. 2007
    ..The large number of hypothetical genes in the CFT073 genome, especially those shown to be UPEC specific, strongly suggests that many urovirulence factors remain uncharacterized...
  24. pmc The role of genomics in the identification, prediction, and prevention of biological threats
    W Florian Fricke
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, USA
    PLoS Biol 7:e1000217. 2009
    ....
  25. pmc Genome sequence of Klebsiella oxytoca 11492-1, a nosocomial isolate possessing a FOX-5 AmpC β-lactamase
    Tracy H Hazen
    Institute for Genome Sciences and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA
    J Bacteriol 194:3028-9. 2012
    ..The K. oxytoca 11492-1 draft genome contains multiple antibiotic resistance genes, including a FOX-5 AmpC β-lactamase encoded on a large IncA/C plasmid...
  26. pmc Draft genome sequences of the diarrheagenic Escherichia coli collection
    Tracy H Hazen
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA
    J Bacteriol 194:3026-7. 2012
    ..net/new/). These genomes represent a valuable resource to the community of researchers who examine these enteric pathogens...
  27. doi request reprint Anti-virulence strategies to combat bacteria-mediated disease
    David A Rasko
    Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Nat Rev Drug Discov 9:117-28. 2010
    ..Here, we discuss the mechanisms of bacterial virulence and present promising anti-virulence strategies and compounds for the future treatment of bacterial infections...
  28. pmc A comparative genomic analysis of diverse clonal types of enterotoxigenic Escherichia coli reveals pathovar-specific conservation
    Jason W Sahl
    University of Maryland School of Medicine, Institute for Genome Sciences, Baltimore, MD, USA
    Infect Immun 79:950-60. 2011
    ..These studies increase our understanding of ETEC evolution, as well as provide insight into virulence factors and conserved proteins, which may be targets for vaccine development...
  29. pmc The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates
    David A Rasko
    Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, 20 Penn Street, Baltimore, MD 21201, USA
    J Bacteriol 190:6881-93. 2008
    ..This comparative study of the species E. coli, while descriptive, should provide the basis for future functional work on this important group of pathogens...
  30. pmc Characterization of Klebsiella sp. strain 10982, a colonizer of humans that contains novel antibiotic resistance alleles and exhibits genetic similarities to plant and clinical Klebsiella isolates
    Tracy H Hazen
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Antimicrob Agents Chemother 58:1879-88. 2014
    ..10982 demonstrated that this organism has acquired genes conferring new metabolic strategies and novel antibiotic resistance alleles, both of which may enhance its ability to colonize the human body. ..
  31. pmc Functional and phylogenetic analysis of ureD in Shiga toxin-producing Escherichia coli
    Susan R Steyert
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201 1509, USA
    J Bacteriol 193:875-86. 2011
    ..coli isolates combined with the ureD sequence reveals that except for a subset of the O157:H7 strains, neither the in vitro urease-positive phenotype nor the ureD sequence is phylogenetically restricted...
  32. pmc Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids
    David A Rasko
    University of Texas Southwestern Medical Center at Dallas, Department of Microbiology, 6000 Harry Hines Blvd, Room NA6 138, Dallas, TX 75235 9048, USA
    Plasmid 58:159-66. 2007
    ..tularensis genetics. Our promoter trap can identify promoters that are activated during intracellular growth and survival. These new vectors provide additional tools for the genetic manipulation of F. tularensis...
  33. pmc The complete genome sequence of Bacillus anthracis Ames "Ancestor"
    Jacques Ravel
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    J Bacteriol 191:445-6. 2009
    ..This study describes the complete genome sequence of that original isolate, derived from a sample kept in cold storage since 1981...
  34. pmc Metabolic analysis of Moraxella catarrhalis and the effect of selected in vitro growth conditions on global gene expression
    Wei Wang
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9048, USA
    Infect Immun 75:4959-71. 2007
    ..These results indicate that growth of M. catarrhalis in a biofilm results in increased expression of gene products which can function not only in energy generation but also in resisting certain elements of the innate immune response...
  35. pmc Native outer membrane proteins protect mice against pulmonary challenge with virulent type A Francisella tularensis
    Jason F Huntley
    Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Infect Immun 76:3664-71. 2008
    ..Taken together, the results of this study demonstrate the potential utility of an OMP subunit (acellular) vaccine for protecting mammals against type A F. tularensis...
  36. ncbi request reprint Bacterial genome sequencing in the clinic: bioinformatic challenges and solutions
    W Florian Fricke
    Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Nat Rev Genet 15:49-55. 2014
    ....
  37. pmc The human microbiome: from symbiosis to pathogenesis
    Emiley A Eloe-Fadrosh
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Annu Rev Med 64:145-63. 2013
    ..In line with these predictions, it is possible that existing conditions may be resolved by altering the microbiota in a positive way...
  38. pmc H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli
    Jonathan A Levine
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America Graduate Program in Biochemistry and Molecular Biology, University of Maryland, Baltimore, Maryland, United States of America
    PLoS ONE 9:e86618. 2014
    ..A regulator present in EPEC, H-NST, positively affects expression of H-NS regulon members in E. coli K-12, although the effect of H-NST on LEE expression and virulence of A/E pathogens has yet-to-be determined...
  39. pmc Patients with Acinetobacter baumannii bloodstream infections are colonized in the gastrointestinal tract with identical strains
    Kerri A Thom
    Division of Healthcare Outcomes Research, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, 100 N Greene Street, Baltimore, MD 21201, USA
    Am J Infect Control 38:751-3. 2010
    ..Seven patients with imipenem-resistant A baumannii bacteremia were identified from January to June of 2008. Six of 7 (86%) patients were colonized in the gastrointestinal tract with genetically similar strains preceding their bacteremia...
  40. pmc Autotransporter genes pic and tsh are associated with Escherichia coli strains that cause acute pyelonephritis and are expressed during urinary tract infection
    Susan R Heimer
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Infect Immun 72:593-7. 2004
    ..There was no significant correlation between cystitis strains (n = 20) and the pic determinant...