Jan Potempa

Summary

Affiliation: University of Georgia
Country: USA

Publications

  1. ncbi request reprint The C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities
    Maryta Sztukowska
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Mol Microbiol 54:1393-408. 2004
  2. ncbi request reprint Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mol Microbiol 57:605-10. 2005
  3. ncbi request reprint Functional and structural characterization of Spl proteases from Staphylococcus aureus
    Grzegorz M Popowicz
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82 152 Martinsried, Germany
    J Mol Biol 358:270-9. 2006
  4. pmc Corruption of innate immunity by bacterial proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 1:70-87. 2009
  5. pmc Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism
    Panagiota G Stathopoulou
    Center for Oral Health and Systemic Disease, School of Dentistry, University of Louisville, Louisville, KY, USA
    BMC Microbiol 9:107. 2009
  6. pmc Bacterial and human peptidylarginine deiminases: targets for inhibiting the autoimmune response in rheumatoid arthritis?
    Pamela Mangat
    The Kennedy Institute of Rheumatology Division, Imperial College, 65 Aspenlea Road, London, W6 8LH, UK
    Arthritis Res Ther 12:209. 2010
  7. ncbi request reprint Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Curr Protein Pept Sci 4:397-407. 2003
  8. pmc Staphylococcus aureus proteases degrade lung surfactant protein A potentially impairing innate immunity of the lung
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 5:251-60. 2013
  9. pmc Inactivation of epidermal growth factor by Porphyromonas gingivalis as a potential mechanism for periodontal tissue damage
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Infect Immun 81:55-64. 2013
  10. ncbi request reprint The staphostatin family of cysteine protease inhibitors in the genus Staphylococcus as an example of parallel evolution of protease and inhibitor specificity
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 388:227-35. 2007

Detail Information

Publications114 found, 100 shown here

  1. ncbi request reprint The C-terminal domains of the gingipain K polyprotein are necessary for assembly of the active enzyme and expression of associated activities
    Maryta Sztukowska
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Mol Microbiol 54:1393-408. 2004
    ..gingivalis outer membrane. Moreover, our results indicate that the immunoglobulin-like subdomain is indispensable for proper folding and expression of the gingipains...
  2. ncbi request reprint Fighting an enemy within: cytoplasmic inhibitors of bacterial cysteine proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mol Microbiol 57:605-10. 2005
    ..This apparently represents a novel system that bacteria use to control the intracellular activity of their proteases...
  3. ncbi request reprint Functional and structural characterization of Spl proteases from Staphylococcus aureus
    Grzegorz M Popowicz
    Max Planck Institute of Biochemistry, Am Klopferspitz 18a, 82 152 Martinsried, Germany
    J Mol Biol 358:270-9. 2006
    ....
  4. pmc Corruption of innate immunity by bacterial proteases
    Jan Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 1:70-87. 2009
    ..It is vital that such mechanisms are elucidated to allow strategies to be developed to aid the innate immune system in controlling bacterial infections...
  5. pmc Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism
    Panagiota G Stathopoulou
    Center for Oral Health and Systemic Disease, School of Dentistry, University of Louisville, Louisville, KY, USA
    BMC Microbiol 9:107. 2009
    ..The oral pathogen Porphyromonas gingivalis has been shown to modulate apoptosis in different cell types, but its effect on epithelial cells remains unclear...
  6. pmc Bacterial and human peptidylarginine deiminases: targets for inhibiting the autoimmune response in rheumatoid arthritis?
    Pamela Mangat
    The Kennedy Institute of Rheumatology Division, Imperial College, 65 Aspenlea Road, London, W6 8LH, UK
    Arthritis Res Ther 12:209. 2010
    ..Blockades of bacterial and human PADs may act as powerful novel therapies by inhibiting the generation of the antigens that trigger and sustain autoimmunity in rheumatoid arthritis...
  7. ncbi request reprint Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Curr Protein Pept Sci 4:397-407. 2003
    ..This hypothetical scenario is a reasonable explanation for the occurrence of many forms of gingipains...
  8. pmc Staphylococcus aureus proteases degrade lung surfactant protein A potentially impairing innate immunity of the lung
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 5:251-60. 2013
    ..Collectively, this study showed that the S. aureus protease ScpA is an important virulence factor that may impair innate immunity of the lungs...
  9. pmc Inactivation of epidermal growth factor by Porphyromonas gingivalis as a potential mechanism for periodontal tissue damage
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Infect Immun 81:55-64. 2013
    ..Cumulatively, these data suggest that the PPAD-activity-abrogating EGF function in gingival pockets may at least partially contribute to tissue damage and delayed healing within P. gingivalis-infected periodontia...
  10. ncbi request reprint The staphostatin family of cysteine protease inhibitors in the genus Staphylococcus as an example of parallel evolution of protease and inhibitor specificity
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 388:227-35. 2007
    ..The tight coevolution is likely the result of the known deleterious effects of uncontrolled staphopain action...
  11. doi request reprint Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus
    Justyna Stec-Niemczyk
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biochem J 419:555-64. 2009
    ..Of interest, even though precise N-terminal trimming is a common regulatory mechanism among S1 family enzymes, the crystal structure of SplA reveals novel significantly different mechanistic details...
  12. pmc Phagocytosis of Staphylococcus aureus by macrophages exerts cytoprotective effects manifested by the upregulation of antiapoptotic factors
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 4:e5210. 2009
    ..Ultimately, it is our contention that this process may contribute to the systemic dissemination of S. aureus infection...
  13. doi request reprint Substrate specificity of Staphylococcus aureus cysteine proteases--Staphopains A, B and C
    Magdalena Kalińska
    Department of Microbiology, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 94:318-27. 2012
    ..However, we show that at the same time the overall specificity of staphopains is relaxed, insofar as multiple substrates that diverge from the sequences described above are also efficiently hydrolyzed...
  14. pmc Roles of the host oxidative immune response and bacterial antioxidant rubrerythrin during Porphyromonas gingivalis infection
    Piotr Mydel
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS Pathog 2:e76. 2006
    ..gingivalis by exacerbating local and systemic inflammation, thereby contributing to the morbidity and mortality associated with infection...
  15. ncbi request reprint Genetic characterization of staphopain genes in Staphylococcus aureus
    Ewa Golonka
    Department of Microbiology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Biol Chem 385:1059-67. 2004
    ....
  16. pmc Biochemical and structural characterization of SplD protease from Staphylococcus aureus
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 8:e76812. 2013
    ..56 Å and 2.1 Å. Molecular modeling and mutagenesis studies allowed us to define a consensus model of substrate binding, and illustrated the molecular mechanism of protease specificity. ..
  17. pmc Citrullination alters immunomodulatory function of LL-37 essential for prevention of endotoxin-induced sepsis
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Immunol 192:5363-72. 2014
    ..Because LL-37 is a potential treatment for sepsis, its administration should be preceded by a careful analysis to ensure that the citrullinated peptide is not generated in treated patients. ..
  18. ncbi request reprint Characterisation of a highly specific, endogenous inhibitor of cysteine protease from Staphylococcus epidermidis, a new member of the staphostatin family
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biol Chem 385:543-6. 2004
    ..The difference in the susceptibility of individual inhibitors to proteolytic cleavage at the reactive site suggests subtle variations in the mechanism of interaction with cysteine proteases...
  19. doi request reprint Staphylococcal cysteine protease staphopain B (SspB) induces rapid engulfment of human neutrophils and monocytes by macrophages
    Jan Smagur
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 386 Krakow, Poland
    Biol Chem 390:361-71. 2009
    ..Collectively, this may lead to the depletion of functional neutrophils at the site of infection, thus facilitating staphylococcal colonisation and spreading...
  20. pmc Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis
    Halina Wojtowicz
    Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
    PLoS Pathog 5:e1000419. 2009
    ..It is also stable against thermal and chemical denaturation. In conclusion, these studies reveal novel molecular properties of HmuY that are consistent with its role as a putative virulence factor during bacterial infection...
  21. pmc Gingipains: Critical Factors in the Development of Aspiration Pneumonia Caused by Porphyromonas gingivalis
    Małgorzata Benedyk
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 8:185-98. 2016
    ..gingivalis-related morbidity and mortality than lysine-specific gingipains. Thus, inhibition of gingipain may be a useful adjunct treatment for P. gingivalis-mediated aspiration pneumonia. ..
  22. pmc A novel matrix metalloprotease-like enzyme (karilysin) of the periodontal pathogen Tannerella forsythia ATCC 43037
    Abdulkarim Y Karim
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow 30 387, Poland
    Biol Chem 391:105-17. 2010
    ..This specificity and the ability to degrade elastin, fibrinogen and fibronectin may contribute to the pathogenicity of periodontitis...
  23. pmc Proteolytic inactivation of LL-37 by karilysin, a novel virulence mechanism of Tannerella forsythia
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland joanna koziel uj edu pl
    J Innate Immun 2:288-93. 2010
    ..Consequently, the karilysin protease may directly contribute to periodontal tissue damage and the development and/or progression of chronic periodontitis...
  24. doi request reprint A new pathway of staphylococcal pathogenesis: apoptosis-like death induced by Staphopain B in human neutrophils and monocytes
    Jan Smagur
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 1:98-108. 2009
    ..We conclude that SspB, particularly in the presence of staphylococcal protein A, may reduce the number of functional phagocytes at infection sites, thus facilitating colonization and dissemination of S. aureus...
  25. pmc Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms
    Maria Rapala-Kozik
    Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    Infect Immun 79:797-805. 2011
    ..Taken together, these data show the importance of this specific type of bacterial surface-host homeostatic system interaction in periodontal infections...
  26. pmc Regulation of chemerin chemoattractant and antibacterial activity by human cysteine cathepsins
    Paulina Kulig
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Immunol 187:1403-10. 2011
    ..Cathepsins may therefore contribute to host defense by activating chemerin to directly inhibit bacterial growth and to recruit pDC to sites of infection...
  27. ncbi request reprint A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus
    Grzegorz Dubin
    Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biochemistry 42:13449-56. 2003
    ....
  28. ncbi request reprint Effects of elastase and cathepsin G on the levels of membrane and soluble TNFalpha
    Renata Mezyk-Kopec
    Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 386:801-11. 2005
    ..Taken together, the ability of NE and CG to modulate levels of membrane and soluble forms of TNFalpha may contribute to the proinflammatory activity of neutrophils...
  29. ncbi request reprint Proteolysis of interleukin-6 receptor (IL-6R) by Porphyromonas gingivalis cysteine proteinases (gingipains) inhibits interleukin-6-mediated cell activation
    Aneta Oleksy
    Institute of Molecular Biology, Jagellonian University, Krakow, Poland
    Microb Pathog 32:173-81. 2002
    ..gingivalis the inflammatory reactions dependent on IL-6 could be severely hindered contributing to both tissue damage and periodontopathogen survival...
  30. pmc Calcium Regulates the Activity and Structural Stability of Tpr, a Bacterial Calpain-like Peptidase
    Dominika Staniec
    From the Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland, Department of Laboratory Medicine, Division of Clinical Chemistry and Pharmacology, Lund University, Lund, 22 100 Sweden
    J Biol Chem 290:27248-60. 2015
    ..Taken together, these results indicate that Tpr likely represents an important pathogenesis factor for P. gingivalis...
  31. pmc The Janus face of α-toxin: a potent mediator of cytoprotection in staphylococci-infected macrophages
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Innate Immun 7:187-98. 2015
    ..Given that the intracellular survival of S. aureus depends on α-toxin, we propose a novel role for this agent in the protection of the intracellular niche, and further dissemination of staphylococci by infected macrophages...
  32. doi request reprint Prevalence of genes encoding extracellular proteases in Staphylococcus aureus - important targets triggering immune response in vivo
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    FEMS Immunol Med Microbiol 66:220-9. 2012
    ..Overall, the results presented in this study support the hypothesis that the secretory proteases as a group may contribute to the virulence of S. aureus...
  33. pmc The role of Mcl-1 in S. aureus-induced cytoprotection of infected macrophages
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mediators Inflamm 2013:427021. 2013
    ....
  34. pmc Porphyromonas gingivalis HmuY and HmuR: further characterization of a novel mechanism of heme utilization
    Teresa Olczak
    Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
    Arch Microbiol 189:197-210. 2008
    ....
  35. doi request reprint Enzymatic activity of the Staphylococcus aureus SplB serine protease is induced by substrates containing the sequence Trp-Glu-Leu-Gln
    Grzegorz Dubin
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    J Mol Biol 379:343-56. 2008
    ..Finally, to guide future development, we attempt to point at likely physiological substrates and thus the role of SplB in staphylococcal physiology...
  36. pmc Inhibition of Staphylococcus aureus cysteine proteases by human serpin potentially limits staphylococcal virulence
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Biol Chem 392:483-9. 2011
    ..aureus-derived inhibitors of the staphopains (staphostatins). Given that S. aureus is a major pathogen of epithelial surfaces, we suggest that SCCA1 functions to temper the virulence of this bacterium by inhibiting the staphopains...
  37. doi request reprint Porphyromonas gingivalis enzymes enhance infection with human metapneumovirus in vitro
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    J Gen Virol 92:2324-32. 2011
    ..In contrast to previously published data for influenza virus, however, Staphylococcus aureus proteases and human neutrophil elastase did not affect virus replication...
  38. pmc Staphylococcal SplB serine protease utilizes a novel molecular mechanism of activation
    Katarzyna Pustelny
    From the Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland, the Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Biol Chem 289:15544-53. 2014
    ..The results allow us to propose a consistent model where changes in the overall protein dynamics rather than structural rearrangement of the active site are involved in the activation process. ..
  39. pmc Degradation of human antimicrobial peptide LL-37 by Staphylococcus aureus-derived proteinases
    Magdalena Sieprawska-Lupa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
    Antimicrob Agents Chemother 48:4673-9. 2004
    ..Taken together, these data suggest that aureolysin production by S. aureus contributes to the resistance of this pathogen to the innate immune system of humans mediated by LL-37...
  40. ncbi request reprint Bacterial proteases in disease - role in intracellular survival, evasion of coagulation/ fibrinolysis innate defenses, toxicoses and viral infections
    Grzegorz Dubin
    Departments of Microbiology, Jagiellonian University, Krakow, Poland
    Curr Pharm Des 19:1090-113. 2013
    ..As such, the potential benefits of pharmacological interference in relevant pathways for the struggle against bacterial pathogens are also discussed...
  41. pmc Inactivation of membrane tumor necrosis factor alpha by gingipains from Porphyromonas gingivalis
    Renata Mezyk-Kopec
    Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Cracow, Poland
    Infect Immun 73:1506-14. 2005
    ..Thus, gingipains are able not only to cleave soluble TNF-alpha but also to destroy the membrane form of the cytokine, which may additionally dysregulate the cytokine network...
  42. ncbi request reprint Friendly fire against neutrophils: proteolytic enzymes confuse the recognition of apoptotic cells by macrophages
    Krzysztof Guzik
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Biochimie 90:405-15. 2008
    ....
  43. doi request reprint Papain-like proteases of Staphylococcus aureus
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Adv Exp Med Biol 712:1-14. 2011
    ..Since these systems are highly conserved, this points to the importance of these proteases in the survival and/or pathogenicity of S. aureus...
  44. pmc Miropin, a novel bacterial serpin from the periodontopathogen Tannerella forsythia, inhibits a broad range of proteases by using different peptide bonds within the reactive center loop
    Miroslaw Ksiazek
    From the Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Biol Chem 290:658-70. 2015
    ..Alternatively, it may act as a housekeeping protein that regulates the activity of endogenous T. forsythia serine proteases. ..
  45. doi request reprint A6.1 "Intratracheal inoculation of porphyromonas gingivalis induces inflammatory changes in the joints of DBA/1 mice"
    Karina Adamowicz
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Ann Rheum Dis 73:A70-1. 2014
    ..Moreover, our findings shown that Porphyromonas gingivalis, the major etiologic factor in PD, facilitates the development and progression of collagen induced arthritis...
  46. ncbi request reprint Growth phase-dependent production of a cell wall-associated elastinolytic cysteine proteinase by Staphylococcus epidermidis
    Aneta Oleksy
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 385:525-35. 2004
    ..This enzyme, with elastinolytic properties, as well as the ability to cleave alpha1PI, fibrinogen and fibronectin, may possibly contribute to the invasiveness and pathogenic potential of S. epidermidis...
  47. doi request reprint Secretory leukocyte proteinase inhibitor-competent DNA deposits are potent stimulators of plasmacytoid dendritic cells: implication for psoriasis
    Joanna Skrzeczyńska-Moncznik
    Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Immunol 189:1611-7. 2012
    ..IFNI synthesis by stimulated pDCs was dependent on intracellular DNA receptor TLR9. Thus, SLPI may contribute to psoriasis by enabling pDCs to sense extracellular DNA and produce IFNI...
  48. pmc Use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 7:e32582. 2012
    ....
  49. pmc Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function
    Tomasz Kantyka
    Department of Microbiology, Jagiellonian University, Krakow, Poland
    Biochimie 92:1644-56. 2010
    ..In this review we present distribution of protein inhibitors from different families among prokaryotes, describe their mode of action and hypothesize on their role in microbial physiology and interactions with hosts and environment...
  50. ncbi request reprint Interaction of regulators Mdm2 and Mdmx with transcription factors p53, p63 and p73
    Michal Zdzalik
    Department of Microbiology, Faculty of Biochemistry, Jagiellonian University, Krakow, Poland
    Cell Cycle 9:4584-91. 2010
    ..Finally, the contribution of these findings to our understanding of the physiological role of these interactions is discussed...
  51. pmc Functional Analysis of Porphyromonas gingivalis W83 CRISPR-Cas Systems
    Michał Burmistrz
    Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow, Poland
    J Bacteriol 197:2631-41. 2015
    ..Four spacers match genomic sequences of the host; however, none of these is flanked at its 3' terminus by the appropriate PAM element...
  52. pmc Human coronavirus NL63 utilizes heparan sulfate proteoglycans for attachment to target cells
    Aleksandra Milewska
    Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Virol 88:13221-30. 2014
    ..In contrast, binding of HCoV-NL63 to heparan sulfates was required for viral attachment and infection of target cells, showing that these molecules serve as attachment receptors for HCoV-NL63...
  53. pmc Pyocycanin, a contributory factor in haem acquisition and virulence enhancement of Porphyromonas gingivalis in the lung
    Malgorzata Benedyk
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 10:e0118319. 2015
    ..gingivalis and disease severity when co-infecting with P. aeruginosa in the lung. ..
  54. doi request reprint HexaPrime: a novel method for detection of coronaviruses
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    J Virol Methods 188:29-36. 2013
    ..The method has been tested for coronaviruses, although it may be also adopted for other RNA viruses...
  55. pmc New method for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates
    Artur Sabat
    Department of Microbiology, Institute of Molecular Biology and Biotechnology, Jagiellonian University, Cracow, Poland
    J Clin Microbiol 41:1801-4. 2003
    ..aureus strains with the discriminatory power and reproducibility of pulsed-field gel electrophoresis while at the same time being rapid and applicable to analysis of large numbers of isolates...
  56. ncbi request reprint Staphostatins: an expanding new group of proteinase inhibitors with a unique specificity for the regulation of staphopains, Staphylococcus spp. cysteine proteinases
    Malgorzata Rzychon
    Department of Analytical Biochemistry, Faculty of Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387 Krakow, Poland
    Mol Microbiol 49:1051-66. 2003
    ....
  57. ncbi request reprint Bacterial peptidases
    Jan Potempa
    Department of Microbiology, Faculty of Biotechnology, Jagiellonian University, PL 30 387 Krakow, Poland
    Contrib Microbiol 12:132-80. 2005
  58. ncbi request reprint Staphylococcus aureus-derived staphopain B, a potent cysteine protease activator of plasma chemerin
    Paulina Kulig
    Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Ulica Gronostajowa 7, 30 387 Krakow, Poland
    J Immunol 178:3713-20. 2007
    ..SspB may help direct the recruitment of specialized host cells, including immunoregulatory pDC and/or macrophages, contributing to the ability of S. aureus to elicit and maintain a chronic inflammatory state...
  59. pmc A potential new pathway for Staphylococcus aureus dissemination: the silent survival of S. aureus phagocytosed by human monocyte-derived macrophages
    Malgorzata Kubica
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS ONE 3:e1409. 2008
    ..S. aureus persists inside macrophages for several days without affecting the viability of these mobile cells which may serve as vehicles for the dissemination of infection...
  60. doi request reprint Purification and characterization of gingipains
    Jan Potempa
    Jagiellonian University, Krakow, Poland
    Curr Protoc Protein Sci . 2007
    ..Obtained from these procedures, the gingipains are stable and can be stored at -80 degrees C for years without loss of activity...
  61. pmc Inhibition of tumor necrosis factor-alpha-induced RANTES secretion by alkaline protease in A549 cells
    Thomas M Krunkosky
    Department of Anatomy and Radiology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30606, USA
    Am J Respir Cell Mol Biol 33:483-9. 2005
    ..These results demonstrate that AP stimulates shedding of cell-surface TNFR1, resulting in an increase in sTNFR1. Consequently, these events decrease the cells' ability to stimulate RANTES gene expression and secretion through TNFR1...
  62. pmc Investigations into sigmaB-modulated regulatory pathways governing extracellular virulence determinant production in Staphylococcus aureus
    Lindsey N Shaw
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA
    J Bacteriol 188:6070-80. 2006
    ..Thus, we propose that the loss of individual genes in this chromosomal hot spot region results in a destabilization of cellular harmony and disruption of the sigmaB regulatory cascade...
  63. pmc Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-Negative bacteria?
    Ky Anh Nguyen
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Science Bldg, Rm A322, Athens, GA 30602, USA
    J Bacteriol 189:833-43. 2007
    ..This result may have a wider implication in a novel secretory pathway in distinct members of the Cytophaga-Flavobacterium-Bacteroidetes phylum...
  64. pmc Peptidyl arginine deiminase from Porphyromonas gingivalis abolishes anaphylatoxin C5a activity
    Ewa Bielecka
    From the Department of Laboratory Medicine, Medical Protein Chemistry, Lund University, SE 205 02 Malmo, Sweden, The Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    J Biol Chem 289:32481-7. 2014
    ..gingivalis, our findings support a crucial role of PPAD in the virulence of P. gingivalis. ..
  65. pmc Gingival Fibromatosis with Significant De Novo Formation of Fibrotic Tissue and a High Rate of Recurrence
    Katarzyna Gawron
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Am J Case Rep 17:655-9. 2016
    ..Further studies are needed to explain this association and improve the management of this condition. ..
  66. pmc The nucleocapsid protein of human coronavirus NL63
    Kaja Zuwała
    Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387, Krakow, Poland
    PLoS ONE 10:e0117833. 2015
    ..Furthermore, no significant alteration in cell cycle progression in cells expressing the protein was observed. This is in stark contrast with results obtained for other coronaviruses, except for the SARS-CoV. ..
  67. pmc Protease-armed bacteria in the skin
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387, Krakow, Poland
    Cell Tissue Res 351:325-37. 2013
    ..Together, this provides protection for colonising and/or invading pathogens from attack by antibacterial forces of the skin...
  68. pmc Porphyromonas gingivalis facilitates the development and progression of destructive arthritis through its unique bacterial peptidylarginine deiminase (PAD)
    Katarzyna J Maresz
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    PLoS Pathog 9:e1003627. 2013
    ..gingivalis. Together, these results suggest bacterial PAD as the mechanistic link between P. gingivalis periodontal infection and rheumatoid arthritis. ..
  69. doi request reprint Novel polymeric inhibitors of HCoV-NL63
    Aleksandra Milewska
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Antiviral Res 97:112-21. 2013
    ..Furthermore, both compounds showed prominent activity against murine hepatitis virus, suggesting broader anticoronaviral activity...
  70. doi request reprint Human SCCA serpins inhibit staphylococcal cysteine proteases by forming classic "serpin-like" covalent complexes
    Tomasz Kantyka
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
    Methods Enzymol 499:331-45. 2011
    ..Anticancer Res.16, 2149-2153.], all of which are regularly colonized by S. aureus, the physiological relevance of SCCA1-staphopain B interaction as a defense mechanism seems to be very well substantiated...
  71. pmc The link between periodontal disease and rheumatoid arthritis: an updated review
    Joanna Koziel
    Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30 387, Krakow, Poland
    Curr Rheumatol Rep 16:408. 2014
    ..In this review, we discuss evidence that PPAD may represent a mechanistic link between periodontitis and RA, diseases that are known to be significantly associated at the epidemiological level. ..
  72. pmc Protease-dependent mechanisms of complement evasion by bacterial pathogens
    Michal Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 393:873-88. 2012
    ....
  73. ncbi request reprint Role of rubrerythrin in the oxidative stress response of Porphyromonas gingivalis
    Maryta Sztukowska
    Institute of Molecular Biology, Jagiellonian University, 31 120 Krakow, Poland
    Mol Microbiol 44:479-88. 2002
    ..Porphyromonas gingivalis contains a superoxide dismutase but lacks catalase and haem peroxidases. We therefore suggest that rubrerythrin provides oxidative stress protection via catalytic reduction of intracellular hydrogen peroxide...
  74. ncbi request reprint Attenuated kinin release from human neutrophil elastase-pretreated kininogens by tissue and plasma kallikreins
    Robert Dulinski
    Faculty of Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Biol Chem 384:929-37. 2003
    ..These results suggest that elastase may be involved in quenching the kinin-release cascade at the late stages of the inflammatory reaction...
  75. ncbi request reprint Poison-antidote systems in bacteria: the co-evolution of functional counterparts
    Jan Potempa
    Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    Cell Mol Biol (Noisy-le-grand) 52:18-22. 2006
    ....
  76. pmc Infection with human coronavirus NL63 enhances streptococcal adherence to epithelial cells
    Anna Golda
    Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
    J Gen Virol 92:1358-68. 2011
    ..The enhanced binding of bacteria correlated with an increased expression level of the platelet-activating factor receptor (PAF-R), but detailed evaluation of the bacterium-PAF-R interaction revealed a limited relevance of this process...
  77. doi request reprint Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63
    Krzysztof Pyrc
    Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
    J Virol Methods 175:133-6. 2011
    ..The detection limit was found to be 1 copy of RNA template per reaction in cell culture supernatants and clinical specimens...
  78. ncbi request reprint The Clostridium ramosum IgA proteinase represents a novel type of metalloendopeptidase
    Klaudia Kosowska
    Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus C DK 8000, Denmark
    J Biol Chem 277:11987-94. 2002
    ..The GC percentage of the iga gene is significantly higher than that for the entire genome of C. ramosum, suggesting that the gene was acquired recently in evolution...
  79. pmc Hydrolysis of epithelial junctional proteins by Porphyromonas gingivalis gingipains
    Jannet Katz
    Department of Oral Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Infect Immun 70:2512-8. 2002
    ..gingivalis...
  80. pmc Proteolysis of CD14 on human gingival fibroblasts by arginine-specific cysteine proteinases from Porphyromonas gingivalis leading to down-regulation of lipopolysaccharide-induced interleukin-8 production
    Hiroyuki Tada
    Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980 8575, Japan
    Infect Immun 70:3304-7. 2002
    ....
  81. ncbi request reprint Inhibition of arginine gingipains (RgpB and HRgpA) with benzamidine inhibitors: zinc increases inhibitory potency
    Joel A Krauser
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30093 0400, USA
    Biol Chem 383:1193-8. 2002
    ..In summary, we have discovered a new series of effective inhibitors for the gingipains and found a novel way to increase inhibitor potency with the HRgpA and RgpB gingipains using zinc...
  82. ncbi request reprint Sequential autolytic processing activates the zymogen of Arg-gingipain
    Jowita Mikolajczyk
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 278:10458-64. 2003
    ..Each step in activation requires the previous step, and an affinity probe reveals that incremental activity enhancements are achieved in a stepwise manner...
  83. ncbi request reprint The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    J Biol Chem 278:40959-66. 2003
    ..Mutations in this residue lead to a loss of affinity of the inhibitor for protease and convert the inhibitor into a substrate...
  84. ncbi request reprint Three-dimensional structure of MecI. Molecular basis for transcriptional regulation of staphylococcal methicillin resistance
    Raquel García-Castellanos
    Institut de Biologia Molecular de Barcelona, Centre d Investigació i Desenvolupament Consell Superior d Investigacions Científiques C Jordi Girona, 18 26, 08034 Barcelona, Spain
    J Biol Chem 278:39897-905. 2003
    ....
  85. ncbi request reprint Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes
    Nafisa Ally
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Biochemistry 42:11693-700. 2003
    ....
  86. ncbi request reprint The biphasic virulence activities of gingipains: activation and inactivation of host proteins
    Takahisa Imamura
    Division of Molecular Pathology, Kumamoto University Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Curr Protein Pept Sci 4:443-50. 2003
    ..gingivalis. Gingipains are potent virulence factors of P. gingivalis, and in many regards their pathogenic activities constitute new mechanisms of bacterial virulence...
  87. ncbi request reprint The role and regulation of the extracellular proteases of Staphylococcus aureus
    Lindsey Shaw
    Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:217-28. 2004
    ..It is likely the attenuation observed in the sspA strain is due to polarity on the sspB gene...
  88. ncbi request reprint 1H, 15N and 13C NMR resonance assignments of staphostatin A, a specific Staphylococcus aureus cysteine proteinase inhibitor
    Grzegorz Dubin
    J Biomol NMR 28:295-6. 2004
    ..Here we report the (1)H, (15)N and (13)C NMR resonance assignments of staphostatin A...
  89. ncbi request reprint Aza-peptide Michael acceptors: a new class of inhibitors specific for caspases and other clan CD cysteine proteases
    Ozlem Dogan Ekici
    School of Chemistry and Biochemistry and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    J Med Chem 47:1889-92. 2004
    ..Aza-Lys and aza-Orn derivatives were potent inhibitors of gingipain K and clostripain. Aza-peptide Michael acceptors showed no cross reactivity toward papain, cathepsin B, and calpain...
  90. ncbi request reprint Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    Biochemistry 43:14306-15. 2004
    ..In a modeled complex of prostaphopain B with staphostatin B, clashes occur both inside and outside the active site cleft, but involve mostly poorly ordered regions of the protein that may be mobile...
  91. ncbi request reprint Coordinate expression of the Porphyromonas gingivalis lysine-specific gingipain proteinase, Kgp, arginine-specific gingipain proteinase, RgpA, and the heme/hemoglobin receptor, HmuR
    Xinyan Liu
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Biol Chem 385:1049-57. 2004
    ..Collectively, these results indicate that kgp , rgpA and hmuR gene transcription is coordinately regulated and may facilitate greater efficiency of heme utilization in P. gingivalis ...
  92. ncbi request reprint A comparison of staphostatin B with standard mechanism serine protease inhibitors
    Renata Filipek
    International Institute of Molecular and Cell Biology, ul Trojdena 4, 02 109 Warsaw, Poland
    J Biol Chem 280:14669-74. 2005
    ....
  93. pmc Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotype
    Lindsey N Shaw
    Department of Biochemistry and Molecular Biology, University of Georgia, Life Sciences Bldg, Athens, GA 30602, USA
    J Bacteriol 187:1751-62. 2005
    ..Seemingly, some of these proteins may play a role in protein secretion; hence, their proteolytic inactivation by SspB has pleiotropic effects on the SspC-deficient mutant...
  94. pmc Gingipains from Porphyromonas gingivalis W83 induce cell adhesion molecule cleavage and apoptosis in endothelial cells
    Shaun M Sheets
    Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Infect Immun 73:1543-52. 2005
    ..Taken together, these results indicate that gingipains from P. gingivalis can alter cell adhesion molecules and induce endothelial cell death, which could have implications for the pathogenicity of this organism...
  95. ncbi request reprint Neuropeptide release from dental pulp cells by RgpB via proteinase-activated receptor-2 signaling
    Salunya Tancharoen
    Department of Restorative Dentistry and Endodontology, Laboratory of Vascular Medicine, Kagoshima University Graduate School of Medical and Dental Science, Japan
    J Immunol 174:5796-804. 2005
    ..This new RgpB activity suggests a possible link between periodontitis and pulp inflammation, which may be modulated by neuropeptides released in the lesion...
  96. pmc Induction of vascular leakage through release of bradykinin and a novel kinin by cysteine proteinases from Staphylococcus aureus
    Takahisa Imamura
    Division of Molecular Pathology, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    J Exp Med 201:1669-76. 2005
    ..aureus virulence and bacterial shock. Therefore, staphopain-specific inhibitors and kinin-receptor antagonists could be used to treat this disease...
  97. ncbi request reprint Exploring the Sn binding pockets in gingipains by newly developed inhibitors: structure-based design, chemistry, and activity
    Arkadiusz Białas
    Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50 370 Wrocław, Poland
    J Med Chem 49:1744-53. 2006
    ..The detailed analysis of Sn binding pockets revealed the molecular basis of inhibitory affinity and provided insight into the structure-activity relationship...
  98. ncbi request reprint Selective inhibition of Porphyromonas gingivalis growth by a factor Xa inhibitor, DX-9065a
    Kenji Matsushita
    Department of Oral Disease Research, National Institute for Longevity Science, Aichi, Japan
    J Periodontal Res 41:171-6. 2006
    ..Porphyromonas gingivalis is a causative bacterium of adult periodontitis. However, there is no drug specific for P. gingivalis and for its virulence factor...
  99. pmc Gingipains from Porphyromonas gingivalis W83 synergistically disrupt endothelial cell adhesion and can induce caspase-independent apoptosis
    Shaun M Sheets
    Department of Biochemistry and Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA
    Infect Immun 74:5667-78. 2006
    ..Degradation of integrin beta1 was inhibited only in the presence of z-VAD-FMK. These results further clarify the role P. gingivalis plays in tissue destruction occurring in the periodontal pocket...
  100. ncbi request reprint Biphasic effect of gingipains from Porphyromonas gingivalis on the human complement system
    Katarzyna Popadiak
    Lund University, Department of Laboratory Medicine, Section of Medical Protein Chemistry, University Hospital Malmo, Malmo, Sweden
    J Immunol 178:7242-50. 2007
    ..At later stages of infection the concentration of proteases is high enough to destroy complement factors and thus render the bacteria resistant to the bactericidal activity of complement...
  101. ncbi request reprint Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the haem-containing pigment from oxyhaemoglobin
    John W Smalley
    Department of Clinical Dental Sciences, The University of Liverpool, Liverpool, UK
    Arch Biochem Biophys 465:44-9. 2007
    ..W. Smalley, M.F. Thomas, A.J. Birss, R. Withnall, J. Silver, Biochem. J. 379 (2004) 833-840.] of the requirement for a combination of both R- and K-gingipains for pigment production from oxyhaemoglobin by P. gingivalis...