Genomes and Genes
Affiliation: University of Virginia
- Distinct functional roles of cytoplasmic dynein defined by the intermediate chain isoformsK Kevin Pfister
Cell Biology Department School of Medicine University of Virginia, PO Box 800732, Charlottesville, VA 22908, United States Electronic address
Exp Cell Res 334:54-60. 2015..Some of the phospho-isoforms of the intermediate chain also specify binding to specific cargo. These data support the model that cytoplasmic dynein can be specifically regulated through the different isoforms of the subunits. ..
- A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomesJunghoon Ha
Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
J Cell Biol 181:1027-39. 2008..These data demonstrate that the IC isoforms define dynein populations that are selectively recruited to transport distinct cargoes...
- Identification and molecular characterization of the p24 dynactin light chainK K Pfister
Department of Cell Biology, University of Virginia Health Science Center, Charlottesville 22908 0439, USA
Cell Motil Cytoskeleton 41:154-67. 1998....
- Genetic analysis of the cytoplasmic dynein subunit familiesK Kevin Pfister
Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
PLoS Genet 2:e1. 2006....
- Cytoplasmic dynein nomenclatureK Kevin Pfister
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Cell Biol 171:411-3. 2005..This nomenclature recognizes the two distinct cytoplasmic dynein complexes and has the flexibility to accommodate the discovery of new subunits and isoforms...
- The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3Kevin W H Lo
Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908 0732, USA
J Biol Chem 282:11205-12. 2007....
- The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chainStephen J Susalka
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
J Biol Chem 277:32939-46. 2002..In addition, this roadblock-binding domain is significantly downstream from both the Tctex1- and LC8-binding sites, supporting the hypothesis that multiple light chain family members can bind to the same intermediate chain...
- Methods to study the interactions of the dynein light chains and intermediate chainsKevin W H Lo
Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA
Methods Mol Biol 392:85-95. 2007..By using several complementary binding methods, we have investigated the protein-protein interaction in the cargo-binding domain of the cytoplasmic dynein...
- Identification of a novel region of the cytoplasmic Dynein intermediate chain important for dimerization in the absence of the light chainsKevin W H Lo
Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 281:9552-9. 2006..Analytical gel filtration and covalent cross-linking of purified recombinant polypeptides further demonstrated that the intermediate chains can dimerize in vitro in the absence of the light chains...
- Intermediate chain subunit as a probe for cytoplasmic dynein function: biochemical analyses and live cell imaging in PC12 cellsKenneth R Myers
Cell Biology Department, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
J Neurosci Res 85:2640-7. 2007..Live cell imaging and comparative immunocytochemical analyses show that dynein is enriched in the actin rich region of growth cones...
- Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complexKevin W H Lo
Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 282:36871-8. 2007..Thus, whereas all combinations of DYNLT light chain dimers can be made, not all of the possible combinations of the isoforms are utilized during the assembly of the dynein complex...
- The Roadblock light chains are ubiquitous components of cytoplasmic dynein that form homo- and heterodimersKarina Nikulina
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
Cell Motil Cytoskeleton 57:233-45. 2004..Immunofluorescence analysis reveals that Robl is present in punctate organelles in rat neuroblastoma cells. In testis, Robl is found in Leydig cells, spermatocytes, and sperm flagella...
- Cytoplasmic dynein mediates adenovirus binding to microtubulesSamir A Kelkar
Weill Medical College of Cornell University, Department of Genetic Medicine, 515 E 71st St, S 1000, New York, NY 10021, USA
J Virol 78:10122-32. 2004..Partially uncoated capsids isolated from cells 40 min after infection also exhibited microtubule binding. In summary, the primary mode of Ad attachment to microtubules occurs though cytoplasmic dynein-mediated binding...
- Viral strategies for intracellular trafficking: motors and microtubulesPhilip L Leopold
Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY, USA
Traffic 7:516-23. 2006....
- Dynein cargo gets its groove backK Kevin Pfister
Structure 13:172-3. 2005..Recent work from the King lab (Wu et al., 2005) on the structure of the Tctex1 dynein light chain provides new insights into the mechanism of cytoplasmic dynein cargo binding and the functional significance of light chain isoform diversity...
- The Role of Cytoplasmic Dynein in Axonal TransportKEVIN PFISTER; Fiscal Year: 2006..These studies will contribute valuable information about the mechanism of axonal transport, a neuronal process important for axon growth and regeneration. ..