Kritika Pershad

Summary

Affiliation: University of Illinois at Chicago
Country: USA

Publications

  1. pmc Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage display
    Kritika Pershad
    Department of Biological Sciences, Laboratory for Molecular Biology M C 567, University of Illinois at Chicago, Molecular Biology Research Building, Chicago, IL 60607, USA
    J Mol Biol 424:88-103. 2012
  2. pmc Drop-out phagemid vector for switching from phage displayed affinity reagents to expression formats
    Kritika Pershad
    Department of Biological Sciences, University of Illinois at Chicago, 900 S Ashland Avenue, Molecular Biology Research Building, Room 4318, Laboratory for Molecular Biology M C 567, Chicago, IL 60607, USA
    Anal Biochem 412:210-6. 2011
  3. pmc A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides
    Leon A Venegas
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    N Biotechnol 33:537-43. 2016
  4. doi Generating thermal stable variants of protein domains through phage display
    Kritika Pershad
    Department of Biological Sciences, University of Illinois at Chicago, 845 W Taylor St, 3240 SES MC 066, Chicago, IL 60607 7060, USA
    Methods 60:38-45. 2013

Collaborators

  • Leon A Venegas
  • Noman Shah
  • Brian K Kay
  • Oluwadamilola Bankole

Detail Information

Publications4

  1. pmc Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage display
    Kritika Pershad
    Department of Biological Sciences, Laboratory for Molecular Biology M C 567, University of Illinois at Chicago, Molecular Biology Research Building, Chicago, IL 60607, USA
    J Mol Biol 424:88-103. 2012
    ..Thus, we have changed the specificity of the FHA1 domain and demonstrated that engineering phosphopeptide-binding domains is an attractive avenue for generating new anti-phosphopeptide binding specificities in vitro by phage display...
  2. pmc Drop-out phagemid vector for switching from phage displayed affinity reagents to expression formats
    Kritika Pershad
    Department of Biological Sciences, University of Illinois at Chicago, 900 S Ashland Avenue, Molecular Biology Research Building, Room 4318, Laboratory for Molecular Biology M C 567, Chicago, IL 60607, USA
    Anal Biochem 412:210-6. 2011
    ..This vector is especially useful in speeding up the downstream screening of affinity reagents and bypassing the time-consuming subcloning experiments...
  3. pmc A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides
    Leon A Venegas
    Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA
    N Biotechnol 33:537-43. 2016
    ..Both the pThr and the residue at the pT+3 position were major factors in defining the specificity of the FHA domains...
  4. doi Generating thermal stable variants of protein domains through phage display
    Kritika Pershad
    Department of Biological Sciences, University of Illinois at Chicago, 845 W Taylor St, 3240 SES MC 066, Chicago, IL 60607 7060, USA
    Methods 60:38-45. 2013
    ..It is anticipated that thermally stable variants will serve as excellent scaffolds for generating affinity reagents to a variety of targets of interest...