Genomes and Genes
Affiliation: University of California
- Electron tomography of mitochondria after the arrest of protein import associated with Tom19 depletionG A Perkins
National Center for Microscopy and Imaging Research, and Department of Neurosciences, University of California San Diego, La Jolla 92093 0608, USA
Eur J Cell Biol 80:139-50. 2001....
- The use of miniSOG in the localization of mitochondrial proteinsGuy A Perkins
National Center for Microscopy and Imaging Research, University of California, San Diego, California, USA Electronic address
Methods Enzymol 547:165-79. 2014..miniSOG is now doing for electron microscopy what the family of green fluorescent protein did for fluorescence microscopy. ..
- The pro-apoptotic Bcl-2 family member tBid localizes to mitochondrial contact sitesM Lutter
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
BMC Cell Biol 2:22. 2001..Once associated with mitochondria, truncated Bid (tBid) causes the potent release of cytochrome c, endonuclease G, and smac...
- Electron tomographic analysis of cytoskeletal cross-bridges in the paranodal region of the node of Ranvier in peripheral nervesGuy A Perkins
National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0608, USA
J Struct Biol 161:469-80. 2008..Interactions between cytoskeleton, membranes, and extracellular matrix associations in the paranodal region are likely critical not only for scaffolding, but also for intracellular and extracellular communication...
- New insights into mitochondrial structure during cell deathGuy Perkins
National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, 92093 0608, USA
Exp Neurol 218:183-92. 2009....
- PKA, PKC, and AKAP localization in and around the neuromuscular junctionG A Perkins
Department of Neurosciences and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA 92093 0608, USA
BMC Neurosci 2:17. 2001..We found previously that a new class of AKAPs, dual-specific AKAPs, denoted D-AKAP1 and D-AKAP2, bind to RIalpha in addition to the RII subunits...
- Three-dimensional structure of the gap junction connexonG Perkins
Department of Neurosciences, University of California, San Diego, La Jolla 92093 0322, USA
Biophys J 72:533-44. 1997..The implication of our hemichannel structure is discussed in relation to the in vivo state of unpaired connexons, which have been shown to exist in the plasma membrane...
- Mitochondrial configurations in peripheral nerve suggest differential ATP productionGuy A Perkins
National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA 92093 0608, United States
J Struct Biol 173:117-27. 2011..The greater density of condensed mitochondria in the PNS axoplasm and in particular at the PNP suggests a need for these organelles to operate at a high workload of ATP production...
- Recent structural insight into mitochondria gained by microscopyG A Perkins
Department of Biology, San Diego State University, CA 92182 4614, USA
Micron 31:97-111. 2000..Sometimes these advances have produced surprising results and more often than not, they have challenged current conceptions of how mitochondria work...
- Electron tomography of neuronal mitochondria: three-dimensional structure and organization of cristae and membrane contactsG Perkins
Biology Department, San Diego State University, California 92115, USA
J Struct Biol 119:260-72. 1997..In addition, a close association was observed between endoplasmic reticulum membranes and the outer mitochondrial membrane, consistent with the reported mechanism of transport of certain lipids into the mitochondrion...
- Perturbations in skeletal muscle sarcomere structure in patients with heart failure and type 2 diabetes: restorative effects of (-)-epicatechin-rich cocoaPam R Taub
School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Clin Sci (Lond) 125:383-9. 2013..These initial results warrant further rigorous investigation, since the use of ERC (or pure epicatechin) may represent a safe and novel means of improving muscle function...
- Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerizationRyuji Yamaguchi
La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA 92037, USA
Mol Cell 31:557-69. 2008..Thus, apoptosis requires a subtle form of Opa1-dependent crista remodeling that is induced by BH3-only proteins and Bax/Bak but independent of MOMP...
- Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membraneTomomi Kuwana
La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Cell 111:331-42. 2002..We conclude that mitochondrial protein release in apoptosis can be mediated by supramolecular openings in the outer mitochondrial membrane, promoted by BH3/Bax/lipid interaction and directly inhibited by Bcl-x(L)...
- Transform-based backprojection for volume reconstruction of large format electron microscope tilt seriesAlbert Lawrence
National Center for Microscopy and Imaging Research, Center for Research in Biological Structure, University of California at San Diego, La Jolla, CA 92093 0608, USA
J Struct Biol 154:144-67. 2006..We will also report on new reconstruction codes and describe our results using these codes...
- Beneficial effects of dark chocolate on exercise capacity in sedentary subjects: underlying mechanisms. A double blind, randomized, placebo controlled trialPam R Taub
University of California, San Diego, School of Medicine, USA
Food Funct 7:3686-93. 2016..Improvements in maximum work achieved and VO2 max may be due to DC activation of upstream control systems and enhancement of SkM mitochondria efficiency. Larger clinical studies are warranted to confirm these observations. ..
- A thermodynamic model describing the nature of the crista junction: a structural motif in the mitochondrionChristian Renken
Department of Biology, San Diego State University, San Diego, CA 92185, USA
J Struct Biol 138:137-44. 2002..We conclude that the crista junction exists predominantly as a circular junction, with other shapes as exceptions made possible by specific characteristics of the lipid bilayer...
- Dynamics of mitochondrial structure during apoptosis and the enigma of Opa1Ryuji Yamaguchi
Burnham Institute for Medical Science, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
Biochim Biophys Acta 1787:963-72. 2009..We review the recent literature pertaining to the coordinated release of cytochrome c during cell death...
- Efficient elimination of cancer cells by deoxyglucose-ABT-263/737 combination therapyRyuji Yamaguchi
Program of Cell Death and Apoptosis, Sanford Burnham Medical Research Institute, La Jolla, California, United States of America
PLoS ONE 6:e24102. 2011....
- Wolfram Syndrome protein, Miner1, regulates sulphydryl redox status, the unfolded protein response, and Ca2+ homeostasisSandra E Wiley
Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA
EMBO Mol Med 5:904-18. 2013..Treatment with the sulphydryl anti-oxidant N-acetylcysteine reversed the abnormalities in the Miner1 deficient cells, suggesting that sulphydryl reducing agents should be explored as a treatment for this rare genetic disease...
- Stimulatory effects of the flavanol (-)-epicatechin on cardiac angiogenesis: additive effects with exerciseIsrael Ramirez-Sanchez
Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
J Cardiovasc Pharmacol 60:429-38. 2012..Thus, the use of Epi may represent a safe and novel means to stimulate myocardial angiogenesis...
- Ultrastructural modifications in the mitochondria of hypoxia-adapted Drosophila melanogasterGuy Perkins
National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, California, United States of America
PLoS ONE 7:e45344. 2012....
- Proteomic and Metabolic Analyses of S49 Lymphoma Cells Reveal Novel Regulation of Mitochondria by cAMP and Protein Kinase AAndrea Wilderman
From the Department of Pharmacology, University of California San Diego, La Jolla, California 92093 0626
J Biol Chem 290:22274-86. 2015..Together, these findings show that cAMP acts via PKA to regulate multiple aspects of mitochondrial function and structure. Mitochondrial perturbation thus likely contributes to cAMP/PKA-mediated cellular responses. ..
- Alterations in skeletal muscle indicators of mitochondrial structure and biogenesis in patients with type 2 diabetes and heart failure: effects of epicatechin rich cocoaPam R Taub
School of Medicine, University of California, San Diego, California, USA
Clin Transl Sci 5:43-7. 2012..Future controlled studies are warranted using Epi-rich cocoa (or pure Epi) to translate improved mitochondrial structure into enhanced cardiac and/or SkM muscle function...
- NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulumL J Huang
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0654, USA
J Cell Biol 145:951-9. 1999..This represents the first example of a differentially targeted AKAP and adds an additional level of complexity to the PKA signaling network...
- Neuronal expression of a functional receptor for the C5a complement activation fragmentJ Caguioa
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Immunol 166:4154-62. 2001..These results have implications for understanding the role of neuronal C5aR receptors in normal neuronal development, neuronal homeostasis, and neuroinflammatory conditions such as Alzheimer's disease...
- Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring proteinL Wang
Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, CA 92093 0654, USA
Proc Natl Acad Sci U S A 98:3220-5. 2001..D-AKAP2 from all three species is highly enriched in mitochondria. The mitochondrial localization and the presence of RGS domains in D-AKAP2 may have important implications for its function in PKA and G protein signal transduction...
- Disruption of mitochondrial function during apoptosis is mediated by caspase cleavage of the p75 subunit of complex I of the electron transport chainJean Ehrland Ricci
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Cell 117:773-86. 2004..Therefore, caspase cleavage of NDUFS1 is required for several mitochondrial changes associated with apoptosis...
- Bcl-xL overexpression blocks bax-mediated mitochondrial contact site formation and apoptosis in rod photoreceptors of lead-exposed miceLihua He
College of Optometry, Department of Biology, University of Houston, Houston, TX 77204, USA
Proc Natl Acad Sci U S A 100:1022-7. 2003..These results have relevance for therapies in a wide variety of progressive retinal and neuronal degenerations where Ca(2+) overload, lead exposure, andor mitochondrial dysfunction occur...
- Three-dimensional analysis of mouse rod and cone mitochondrial cristae architecture: bioenergetic and functional implicationsGuy A Perkins
Department of Neurosciences and National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, CA, USA
Mol Vis 9:60-73. 2003..Therefore, we investigated the oxidative metabolism and three-dimensional membrane architecture of mouse rod and cone inner segment mitochondria...
- Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosisMei G Sun
Department of Biology, San Diego State University, San Diego, CA 92182 4614, USA
Nat Cell Biol 9:1057-65. 2007..Swelling occurs only late in apoptosis after release of cytochrome c and loss of the mitochondrial membrane potential...
- Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapsesJerry E Johnson
Department of Natural Sciences, University of Houston Downtown, Houston, TX, USA
Mol Vis 13:887-919. 2007..The goal of our structural and functional studies was to determine the spatiotemporal regulation of ATP and Ca2+ dynamics in rod spherules and cone pedicles...
- Crosstalk between nitric oxide and zinc pathways to neuronal cell death involving mitochondrial dysfunction and p38-activated K+ channelsElla Bossy-Wetzel
Center for Neuroscience and Aging, The Burnham Institute, La Jolla, CA 92037, USA
Neuron 41:351-65. 2004..Thus, these data establish a new form of crosstalk between NO and Zn2+ apoptotic signal transduction pathways that may contribute to neurodegeneration...
- Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neuronsMark J Barsoum
Apoptosis and Cell Death Program, Burnham Institute for Medical Research, La Jolla, CA, USA
EMBO J 25:3900-11. 2006..Importantly, NO-induced neuronal cell death was mitigated by Mfn1 and Drp1(K38A). Thus, persistent mitochondrial fission may play a causal role in NO-mediated neurotoxicity...
- Elevated hydrostatic pressure triggers mitochondrial fission and decreases cellular ATP in differentiated RGC-5 cellsWon Kyu Ju
Hamilton Glaucoma Center and Department of Ophthalmology, University of California San Diego, La Jolla, California 92037, USA
Invest Ophthalmol Vis Sci 48:2145-51. 2007..The purpose of this study was to determine whether elevated hydrostatic pressure induces mitochondrial fission and dysfunction in cultured retinal ganglion cells...
- Intraocular pressure elevation induces mitochondrial fission and triggers OPA1 release in glaucomatous optic nerveWon Kyu Ju
Department of Ophthalmology, Hamilton Glaucoma Center, School of Medicine, University of California San Diego, La Jolla, California 92037 0946, USA
Invest Ophthalmol Vis Sci 49:4903-11. 2008..To determine whether elevation of intraocular pressure (IOP) triggers mitochondrial fission and ultrastructural changes and alters optic atrophy type 1 (OPA1) expression and distribution in the optic nerve (ON) of glaucomatous DBA/2J mice...