Keith E Mostov

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint ERK and MMPs sequentially regulate distinct stages of epithelial tubule development
    Lucy Erin O'Brien
    Department of Anatomy, Department of Biochemistry and Biophysics and The Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Cell 7:21-32. 2004
  2. pmc From cells to organs: building polarized tissue
    David M Bryant
    Department of Anatomy, University of California San Francisco, California 94143 2140, USA
    Nat Rev Mol Cell Biol 9:887-901. 2008
  3. pmc Cell height: Tao rising
    Liang Cai
    State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China
    J Cell Biol 199:1023-4. 2012
  4. pmc STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis
    Minji Kim
    Department of Anatomy, University of California San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 21:3926-33. 2010
  5. pmc Computational investigation of epithelial cell dynamic phenotype in vitro
    Sean H J Kim
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA
    Theor Biol Med Model 6:8. 2009
  6. pmc A computational approach to resolve cell level contributions to early glandular epithelial cancer progression
    Sean H J Kim
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA
    BMC Syst Biol 3:122. 2009
  7. pmc Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b
    Isla D Cheung
    Department of Biochemistry and Biophysics, Program in Developmental and Stem Cell Biology, and Institute for Regeneration Medicine, University of California, San Francisco, CA 94158, USA
    Dev Biol 361:68-78. 2012
  8. ncbi request reprint Rac1 is required for reorientation of polarity and lumen formation through a PI 3-kinase-dependent pathway
    Kathleen D Liu
    Division of Nephrology, University of California, San Francisco, Box 2140, San Francisco, CA 94143 2140, USA
    Am J Physiol Renal Physiol 293:F1633-40. 2007
  9. pmc Nectin proteins are expressed at early stages of nephrogenesis and play a role in renal epithelial cell morphogenesis
    PAUL R BRAKEMAN
    Division of Pediatric Nephrology, Department of Pediatrics, University of California, Box 0748, San Francisco, California 94143 0748, USA
    Am J Physiol Renal Physiol 296:F564-74. 2009
  10. pmc Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarity
    Wei Yu
    Department of Anatomy, Neurology, University of California, San Francisco, California 94143, USA
    EMBO Rep 9:923-9. 2008

Research Grants

Collaborators

Detail Information

Publications45

  1. ncbi request reprint ERK and MMPs sequentially regulate distinct stages of epithelial tubule development
    Lucy Erin O'Brien
    Department of Anatomy, Department of Biochemistry and Biophysics and The Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Cell 7:21-32. 2004
    ..Thus, ERK and MMPs define two regulatory subprograms that act in sequence. By inducing these reciprocal signals, HGF directs the morphogenetic progression of tubule development...
  2. pmc From cells to organs: building polarized tissue
    David M Bryant
    Department of Anatomy, University of California San Francisco, California 94143 2140, USA
    Nat Rev Mol Cell Biol 9:887-901. 2008
    ....
  3. pmc Cell height: Tao rising
    Liang Cai
    State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, China
    J Cell Biol 199:1023-4. 2012
    ..Their results indicate that Tao is rising as a regulator of cell height...
  4. pmc STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis
    Minji Kim
    Department of Anatomy, University of California San Francisco, San Francisco, CA 94158, USA
    Mol Biol Cell 21:3926-33. 2010
    ..Constitutively active or inactive forms of STAT1 did not promote lumen maturation, suggesting this requires a distinct signal...
  5. pmc Computational investigation of epithelial cell dynamic phenotype in vitro
    Sean H J Kim
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA
    Theor Biol Med Model 6:8. 2009
    ..Characterizing essential cell actions and their roles, which constitute the system's dynamic phenotype, is critical to gaining deeper insight into the cystogenesis phenomena...
  6. pmc A computational approach to resolve cell level contributions to early glandular epithelial cancer progression
    Sean H J Kim
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA
    BMC Syst Biol 3:122. 2009
    ..We explored selective disruption of individual simulated cell actions. New framework features enabled recording detailed measures of ISEA cell activities and morphology...
  7. pmc Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b
    Isla D Cheung
    Department of Biochemistry and Biophysics, Program in Developmental and Stem Cell Biology, and Institute for Regeneration Medicine, University of California, San Francisco, CA 94158, USA
    Dev Biol 361:68-78. 2012
    ..Thus, cldn15lb mutants provide a novel in vivo model to study the role of tight junction proteins in the remodeling of the biliary network and hereditary cholestasis...
  8. ncbi request reprint Rac1 is required for reorientation of polarity and lumen formation through a PI 3-kinase-dependent pathway
    Kathleen D Liu
    Division of Nephrology, University of California, San Francisco, Box 2140, San Francisco, CA 94143 2140, USA
    Am J Physiol Renal Physiol 293:F1633-40. 2007
    ..This effect cannot be overcome by constitutively active Rac1, demonstrating that both Rac1 and atypical PKC are required for reorientation of cellular polarity...
  9. pmc Nectin proteins are expressed at early stages of nephrogenesis and play a role in renal epithelial cell morphogenesis
    PAUL R BRAKEMAN
    Division of Pediatric Nephrology, Department of Pediatrics, University of California, Box 0748, San Francisco, California 94143 0748, USA
    Am J Physiol Renal Physiol 296:F564-74. 2009
    ....
  10. pmc Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarity
    Wei Yu
    Department of Anatomy, Neurology, University of California, San Francisco, California 94143, USA
    EMBO Rep 9:923-9. 2008
    ..These results might be relevant to the hyperactivation of RhoA and disruption of normal polarity frequently observed in human epithelial cancers...
  11. pmc Scrib regulates HGF-mediated epithelial morphogenesis and is stabilized by Sgt1-HSP90
    Dennis J Eastburn
    Department of Anatomy, University of California, San Francisco, CA 94143, USA
    J Cell Sci 125:4147-57. 2012
    ....
  12. pmc Liver progenitor cells fold up a cell monolayer into a double-layered structure during tubular morphogenesis
    Naoki Tanimizu
    Departments of Anatomy, and Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143 2140, USA
    Mol Biol Cell 20:2486-94. 2009
    ....
  13. pmc p120 catenin is required for normal renal tubulogenesis and glomerulogenesis
    DENISE K MARCIANO
    Department of Medicine, University of California, San Francisco, CA 94158, USA
    Development 138:2099-109. 2011
    ..Taken together, our data show that p120ctn is required for early tubule and glomerular morphogenesis, as well as control of luminal diameter, probably through regulation of cadherins...
  14. pmc MDCK cystogenesis driven by cell stabilization within computational analogues
    Jesse A Engelberg
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, University of California, San Francisco, California, USA
    PLoS Comput Biol 7:e1002030. 2011
    ..Reducing the amount of in silico luminal cell death had limited effect on cystogenesis. Simulations provide an observable theory for cystogenesis based on hypothesized, cell-level operating principles...
  15. pmc Molecular regulation of lumen morphogenesis
    Anirban Datta
    Department of Anatomy, University of California San Francisco, CA 94143 2140, USA
    Curr Biol 21:R126-36. 2011
    ..We here discuss progress in understanding common design principles underpinning de novo lumen formation and expansion...
  16. pmc Beta1-integrin orients epithelial polarity via Rac1 and laminin
    Wei Yu
    Department of Anatomy, School of Medicine, University of California San Francisco, San Francisco, CA 94143, USA
    Mol Biol Cell 16:433-45. 2005
    ..Together, our results suggest a signaling pathway in which the activation of beta1-integrins orients the apical pole of polarized cysts via a mechanism that requires Rac1 activation and laminin organization into the basement membrane...
  17. pmc A kinase cascade leading to Rab11-FIP5 controls transcytosis of the polymeric immunoglobulin receptor
    Tao Su
    Department of Anatomy, University of California, San Francisco, CA 94158 2517, USA
    Nat Cell Biol 12:1143-53. 2010
    ..Finally, we demonstrate that Rab11-FIP5 phosphorylation by ERK controls Rab11a endosome distribution and pIgA-pIgR transcytosis. Our results reveal a novel Yes-EGFR-ERK-FIP5 signalling network for regulation of pIgA-pIgR transcytosis...
  18. pmc A molecular network for de novo generation of the apical surface and lumen
    David M Bryant
    Department of Anatomy, University of California, San Francisco, CA 94143 2140, USA
    Nat Cell Biol 12:1035-45. 2010
    ..This Rab11a-directed network directs Cdc42-dependent apical exocytosis during lumen formation, revealing an interaction between the machineries of vesicular transport and polarization...
  19. pmc Transcriptional profiling identifies TNS4 function in epithelial tubulogenesis
    Sang Ho Kwon
    Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143 2140, USA
    Curr Biol 21:161-6. 2011
    ..Exogenous expression of a constitutively active STAT3 mimics the defect by the mutation. Our study highlights the role of the TNS4-STAT3 axis in epithelial sheet invasion and tubulogenesis...
  20. pmc Pak1 and PIX regulate contact inhibition during epithelial wound healing
    Mirjam M P Zegers
    Department of Anatomy and Biochemistry and Biophysics, University of California San Francisco, Box 2140, 600 16th Street, San Francisco, CA 94143, USA
    EMBO J 22:4155-65. 2003
    ..Thus, recruitment of activated Pak and PIX to cell-cell contacts is pivotal to transduction of growth-inhibitory signals from neighboring cells in epithelial wound healing...
  21. pmc Reduced immunoglobulin A transcytosis associated with immunoglobulin A nephropathy and nasopharyngeal carcinoma
    Tao Su
    Department of Anatomy, University of California, San Francisco, California 94158, USA
    J Biol Chem 286:44921-5. 2011
    ..We propose that the defects in pIgR trafficking caused by the A580V mutation may underlie the pathogenesis of both diseases...
  22. ncbi request reprint Polarity proteins PAR6 and aPKC regulate cell death through GSK-3beta in 3D epithelial morphogenesis
    Minji Kim
    Departments of Anatomy, and Biochemistry and Biophysics, University of California School of Medicine, San Francisco, CA 94158, USA
    J Cell Sci 120:2309-17. 2007
    ..We conclude that a PAR6-aPKC-GSK-3beta mechanism links cell polarity and apoptosis...
  23. ncbi request reprint Epithelial polarity and tubulogenesis in vitro
    Mirjam M P Zegers
    Department of Anatomy, Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0452, USA
    Trends Cell Biol 13:169-76. 2003
    ..In vitro systems can be used to study tube formation and might help to define common principles underlying the formation of diverse types of tubular organ...
  24. ncbi request reprint The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor
    Marcel Verges
    Department of Anatomy, University of California, San Francisco, CA 94143 2140, USA
    Nat Cell Biol 6:763-9. 2004
    ..Here, we show a function for the mammalian Vps35-Vps29-Vps26 retromer subcomplex in promoting pIgR-pIgA transcytosis...
  25. pmc Phosphoinositide 3-kinase regulates the role of retromer in transcytosis of the polymeric immunoglobulin receptor
    Marcel Verges
    Department of Anatomy, Cardiovascular Research Institute and Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco, California 94143 2140, USA
    Exp Cell Res 313:707-18. 2007
    ..These data implicate PI3K in controlling retromer's role in pIgR-pIgA transcytosis...
  26. pmc Hepatocyte growth factor switches orientation of polarity and mode of movement during morphogenesis of multicellular epithelial structures
    Wei Yu
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143 0452, USA
    Mol Biol Cell 14:748-63. 2003
    ..Our results demonstrate the power of direct observation in investigating how individual cell behaviors, such as polarization, movement, and division are coordinated in the very complex process of producing multicellular structures...
  27. pmc Slug is required for cell survival during partial epithelial-mesenchymal transition of HGF-induced tubulogenesis
    Pascale Leroy
    Department of Anatomy, University of California, San Francisco, CA 94158 2517, USA
    Mol Biol Cell 18:1943-52. 2007
    ..We conclude that in epithelial cells, promoting cell survival can be a primary function of Slug, rather than being acquired concomitantly with EMT...
  28. pmc Identification of a cytoplasmic signal for apical transcytosis
    Frederic Luton
    Department of Anatomy, and Biochemistry and Biophysics, and Cardiovascular Research Institute, University of California, San Francisco, CA 94158 2140, USA
    Traffic 10:1128-42. 2009
    ..This apical transcytosis sorting signal was not involved in polarized biosynthetic traffic of the pIgR...
  29. pmc Cyclic AMP regulates formation of mammary epithelial acini in vitro
    Pavel I Nedvetsky
    Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143 2140, USA
    Mol Biol Cell 23:2973-81. 2012
    ..These data demonstrate that cAMP regulates lumen formation in mammary epithelial cells in vitro, both through acceleration of polarization of outer cells and apoptosis of inner cells of the acinus...
  30. ncbi request reprint Opinion: Building epithelial architecture: insights from three-dimensional culture models
    Lucy Erin O'Brien
    Department of Anatomy, and the Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    Nat Rev Mol Cell Biol 3:531-7. 2002
    ....
  31. pmc Liver progenitor cells develop cholangiocyte-type epithelial polarity in three-dimensional culture
    Naoki Tanimizu
    Department of Anatomy, University of California San Francisco, San Francisco, CA 94143 2140, USA
    Mol Biol Cell 18:1472-9. 2007
    ....
  32. ncbi request reprint Hepatocyte growth factor induces MDCK cell morphogenesis without causing loss of tight junction functional integrity
    Anne L Pollack
    Department of Anatomy, Department of Biochemistry and Biophysics and Cardiovascular Research Institute, University of California San Francisco School of Medicine, San Francisco, California 94143 2140, USA
    Am J Physiol Cell Physiol 286:C482-94. 2004
    ....
  33. pmc The role of syntaxins in the specificity of vesicle targeting in polarized epithelial cells
    Martin B A ter Beest
    Department of Anatomy and Biochemistry, University of California, San Francisco, San Francisco, CA 94143 2140, USA
    Mol Biol Cell 16:5784-92. 2005
    ..We suggest that syntaxins may play a role in determining the specificity of membrane targeting by permitting fusion with only certain target membranes...
  34. doi request reprint In silico simulation of epithelial cell tubulogenesis
    Jesse A Engelberg
    UCSF UC Berkeley Joint Graduate Group in Bioengineering, San Francisco, CA 94143, USA
    Conf Proc IEEE Eng Med Biol Soc 2008:1036-9. 2008
    ....
  35. pmc mTOR is out of control in polycystic kidney disease
    Keith E Mostov
    Department of Anatomy, School of Medicine, University of California San Francisco, Genentech Hall Room N212B, Box 2140, 600 16th Street, San Francisco, CA 94147, USA
    Proc Natl Acad Sci U S A 103:5247-8. 2006
  36. pmc A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma
    Qi Wen Fan
    Department of Neurology, University of California, San Francisco, San Francisco, California 94143, USA
    Cancer Cell 9:341-9. 2006
    ..PI-103 showed significant activity in xenografted tumors with no observable toxicity. These data demonstrate an emergent efficacy due to combinatorial inhibition of mTOR and PI3 kinase alpha in malignant glioma...
  37. pmc Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection
    Cindy S Tran
    Division of Neonatology, Department of Pediatrics, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Host Microbe 15:636-43. 2014
    ..Our data reveal a role for spatiotemporal epithelial polarity changes in the activation of innate immune responses...
  38. ncbi request reprint Direct interaction between Rab3b and the polymeric immunoglobulin receptor controls ligand-stimulated transcytosis in epithelial cells
    Sven C D Van IJzendoorn
    Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
    Dev Cell 2:219-28. 2002
    ..Overexpression of GTP-locked rab3b inhibits dIgA-stimulated transcytosis. Together, our data demonstrate that a rab protein can bind directly to a specific cargo protein and thereby control its trafficking...
  39. pmc Intercellular Transfer of GPRC5B via Exosomes Drives HGF-Mediated Outward Growth
    Sang Ho Kwon
    Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 24:199-204. 2014
    ....
  40. ncbi request reprint Genetic control of single lumen formation in the zebrafish gut
    Michel Bagnat
    Department of Biochemistry and Biophysics, Programs in Developmental Biology, Genetics and Human Genetics, University of California, San Francisco, 1550 Fourth Street, San Francisco, California 94158 2324, USA
    Nat Cell Biol 9:954-60. 2007
    ..This work shows that single lumen formation is genetically controlled and appears to be driven by the accumulation of fluid...
  41. ncbi request reprint The exocyst affects protein synthesis by acting on the translocation machinery of the endoplasmic reticulum
    Joshua H Lipschutz
    Department of Medicine and the Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 278:20954-60. 2003
    ..Although the exact mechanism remains to be elucidated, the exocyst/Sec61beta interaction represents an important link between the cellular membrane trafficking and protein synthetic machinery...
  42. ncbi request reprint Role of rab proteins in epithelial membrane traffic
    Sven C D Van IJzendoorn
    Department of Membrane Cell Biology, University of Groningen, Groningen 9713AV, The Netherlands
    Int Rev Cytol 232:59-88. 2003
    ..This review discusses the role of rabs in epithelial membrane transport...
  43. ncbi request reprint Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures
    Lucy Erin O'Brien
    Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Methods Enzymol 406:676-91. 2006
    ..These protocols enable the regulation of epithelial morphogenesis to be explored at a detailed molecular level...
  44. ncbi request reprint EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin
    David M Bryant
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    J Cell Sci 120:1818-28. 2007
    ..Together, these findings demonstrate a role for SNX1 in retrieval of E-cadherin from a degradative endosomal pathway and in membrane trafficking pathways that regulate E-cadherin recycling...
  45. ncbi request reprint Epithelial polarity and morphogenesis
    Keith E Mostov
    Methods 30:189-90. 2003

Research Grants56

  1. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2006
    ..We will analyze the effects of this point mutation on plgR trafficking and function in vitro system, using mutant plgR expressed in Madin-Darby canine kidney epithelial cells. ..
  2. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 1991
    ..6) Cell-free assays for pIg-R transport will be developed. 7) The intestinal IgG receptor will be cloned and sequenced, and the cDNA will be expressed in MDCK cells to study the receptor's function...
  3. TRANSPORT OF IMMUNOGOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 1993
    ..7) Transport from the TGN to the basolateral surface will be reconstituted in a perforated cell system. 8) The composition of transcytotic and TGN derived vesicles will be compared...
  4. HOST CELL DETERMINANTS IN PSEUDOMONAS PNEUMONIA
    Keith Mostov; Fiscal Year: 2003
    ..abstract_text> ..
  5. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2001
    ..4). Preliminary data indicate that there are specific substrates for p62Yes and we will identify these and test their roles in stimulation of transcytosis. ..
  6. HOST CELL DETERMINANTS IN PSEUDOMONAS PNEUMONIA
    Keith Mostov; Fiscal Year: 2001
    ..abstract_text> ..
  7. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2003
    ..4). Preliminary data indicate that there are specific substrates for p62Yes and we will identify these and test their roles in stimulation of transcytosis. ..
  8. Mechanisms of Renal Tubulogenesis
    Keith Mostov; Fiscal Year: 2007
    ..We are using a simple model system where kidney cells are grown in culture and produce such tubes, to study tube formation and how we can influence this to treat and prevent kidney diseases. ..
  9. HOST CELL DETERMINANTS IN PSEUDOMONAS PNEUMONIA
    Keith Mostov; Fiscal Year: 1999
    ..abstract_text> ..
  10. Control of Orientation of Epithelial Polarity
    Keith Mostov; Fiscal Year: 2007
    ..This will be invaluable in understanding the pathogenesis of renal diseases where this polarity is deranged. ..
  11. Formation of bile ducts in three dimensional culture
    Keith E Mostov; Fiscal Year: 2010
    ..The proposed work will greatly increase our understanding of early bile duct development and lead to improved health for patients with these diseases. ..
  12. Novel Method for Mucosal Delivery of Protein Drugs
    Keith Mostov; Fiscal Year: 2003
    ..In Specific Aim 2 we will maximize transcytosis by optimizing our new class of ligands. We will vary off rate, pH sensitivity, and the exact region of the pIgR to which they bind. ..
  13. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2004
    ..4). Preliminary data indicate that there are specific substrates for p62Yes and we will identify these and test their roles in stimulation of transcytosis. ..
  14. Control of Orientation of Epithelial Polarity
    Keith Mostov; Fiscal Year: 2004
    ..This will be invaluable in understanding the pathogenesis of renal diseases where this polarity is deranged. ..
  15. Control of Orientation of Epithelial Polarity
    Keith Mostov; Fiscal Year: 2005
    ..This will be invaluable in understanding the pathogenesis of renal diseases where this polarity is deranged. ..
  16. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2005
    ..We will analyze the effects of this point mutation on plgR trafficking and function in vitro system, using mutant plgR expressed in Madin-Darby canine kidney epithelial cells. ..
  17. Mechanisms of Renal Tubulogenesis
    Keith E Mostov; Fiscal Year: 2010
    ..We are using a simple model system where kidney cells are grown in culture and produce such tubes, to study tube formation and how we can influence this to treat and prevent kidney diseases. ..
  18. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2009
    ..We will analyze the effects of this point mutation on plgR trafficking and function in vitro system, using mutant plgR expressed in Madin-Darby canine kidney epithelial cells. ..
  19. Control of Orientation of Epithelial Polarity
    Keith Mostov; Fiscal Year: 2006
    ..This will be invaluable in understanding the pathogenesis of renal diseases where this polarity is deranged. ..
  20. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 2007
    ..The first specific immunological defense against these agents is the transport of immunoglobulin A (IgA) into external mucosal secretions. We are studying how IgA is transported by the polymeric immunoglobulin receptor. ..
  21. HOST CELL DETERMINANTS IN PSEUDOMONAS PNEUMONIA
    Keith Mostov; Fiscal Year: 2002
    ..abstract_text> ..
  22. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2002
    ..4). Preliminary data indicate that there are specific substrates for p62Yes and we will identify these and test their roles in stimulation of transcytosis. ..
  23. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 1999
    ..5) Calmodulin binds with high affinity in a Ca2+ dependent fashion to the region of the cytoplasmic domain of the plgR that controls its polarized sorting. We will investigate the role of Ca2+ and calmodulin in transcytosis of the plgR. ..
  24. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 1999
    ..Their roles in the movement of the pIgR across polarized epithelial cells will be analyzed. Other proteins that interact with these proteins or with the pIgR will also be identified and their roles examined. ..
  25. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 1990
    ..6) Cell-free assays for pIg-R transport will be developed. 7) The intestinal IgG receptor will be cloned and sequenced, and the cDNA will be expressed in MDCK cells to study the receptor's function...
  26. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 2000
    ..Their roles in the movement of the pIgR across polarized epithelial cells will be analyzed. Other proteins that interact with these proteins or with the pIgR will also be identified and their roles examined. ..
  27. HOST CELL DETERMINANTS IN PSEUDOMONAS PNEUMONIA
    Keith Mostov; Fiscal Year: 2000
    ..abstract_text> ..
  28. Novel Method for Mucosal Delivery of Protein Drugs
    Keith Mostov; Fiscal Year: 2004
    ..In Specific Aim 2 we will maximize transcytosis by optimizing our new class of ligands. We will vary off rate, pH sensitivity, and the exact region of the pIgR to which they bind. ..
  29. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 2001
    ..Their roles in the movement of the pIgR across polarized epithelial cells will be analyzed. Other proteins that interact with these proteins or with the pIgR will also be identified and their roles examined. ..
  30. Novel Method for Mucosal Delivery of Protein Drugs
    Keith Mostov; Fiscal Year: 2001
    ..In Specific Aim 2 we will maximize transcytosis by optimizing our new class of ligands. We will vary off rate, pH sensitivity, and the exact region of the pIgR to which they bind. ..
  31. Multiphoton Microscope Facility for UCSF Mission Bay
    Keith Mostov; Fiscal Year: 2003
    ..This new system greatly facilitates the use of fluorophores with closely overlapping spectra (such as different variants of GFP) and should significantly enhance the use of FRET. ..
  32. Mechanisms of Renal Tubulogenesis
    Keith Mostov; Fiscal Year: 2009
    ..We are using a simple model system where kidney cells are grown in culture and produce such tubes, to study tube formation and how we can influence this to treat and prevent kidney diseases. ..
  33. Novel Method for Mucosal Delivery of Protein Drugs
    Keith Mostov; Fiscal Year: 2002
    ..In Specific Aim 2 we will maximize transcytosis by optimizing our new class of ligands. We will vary off rate, pH sensitivity, and the exact region of the pIgR to which they bind. ..
  34. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2007
    ..We will analyze the effects of this point mutation on plgR trafficking and function in vitro system, using mutant plgR expressed in Madin-Darby canine kidney epithelial cells. ..
  35. REGULATION OF TRANSCYTOSIS OF POLYMERIC IMMUNOGLOBULINS
    Keith Mostov; Fiscal Year: 2000
    ..4). Preliminary data indicate that there are specific substrates for p62Yes and we will identify these and test their roles in stimulation of transcytosis. ..
  36. TRANSPORT OF IMMUNOGLOBULINS ACROSS EPITHELIAL CELLS
    Keith Mostov; Fiscal Year: 1992
    ..7) Transport from the TGN to the basolateral surface will be reconstituted in a perforated cell system. 8) The composition of transcytotic and TGN derived vesicles will be compared...
  37. Mechanisms of Renal Tubulogenesis
    Keith E Mostov; Fiscal Year: 2010
    ..We are using a simple model system where kidney cells are grown in culture and produce such tubes, to study tube formation and how we can influence this to treat and prevent kidney diseases. ..