Luis Martinez-Sobrido

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc Generation of recombinant influenza virus from plasmid DNA
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester, USA
    J Vis Exp . 2010
  2. pmc Reverse Genetics Approaches to Control Arenavirus
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA
    Methods Mol Biol 1403:313-51. 2016
  3. pmc Reporter-Expressing, Replicating-Competent Recombinant Arenaviruses
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Viruses 8:. 2016
  4. pmc Arenavirus reverse genetics for vaccine development
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Gen Virol 94:1175-88. 2013
  5. pmc The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer's disease
    Hayk Davtyan
    Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647, USA
    J Transl Med 9:127. 2011
  6. pmc Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals
    Margaret J Wortman
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 W, Olney Road, Norfolk, VA 23507, USA
    BMC Mol Biol 11:81. 2010
  7. pmc D471G mutation in LCMV-NP affects its ability to self-associate and results in a dominant negative effect in viral RNA synthesis
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642, USA
    Viruses 4:2137-61. 2012
  8. pmc Arenavirus nucleoproteins prevent activation of nuclear factor kappa B
    W W Shanaka I Rodrigo
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 86:8185-97. 2012
  9. pmc Self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 86:3307-17. 2012
  10. pmc The C-terminal region of lymphocytic choriomeningitis virus nucleoprotein contains distinct and segregable functional domains involved in NP-Z interaction and counteraction of the type I interferon response
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Virol 85:13038-48. 2011

Collaborators

Detail Information

Publications41

  1. pmc Generation of recombinant influenza virus from plasmid DNA
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester, USA
    J Vis Exp . 2010
    ....
  2. pmc Reverse Genetics Approaches to Control Arenavirus
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY, 14642, USA
    Methods Mol Biol 1403:313-51. 2016
    ..In this chapter we summarize the current knowledge on arenavirus reverse genetics and the implementation of plasmid-based reverse genetics techniques for the development of arenavirus vaccines and vaccine vectors. ..
  3. pmc Reporter-Expressing, Replicating-Competent Recombinant Arenaviruses
    Luis Martinez-Sobrido
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Viruses 8:. 2016
    ....
  4. pmc Arenavirus reverse genetics for vaccine development
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Gen Virol 94:1175-88. 2013
    ..Moreover, we show the feasibility of using these novel strategies for efficient rescue of recombinant tri-segmented both LCMV and Candid#1...
  5. pmc The immunological potency and therapeutic potential of a prototype dual vaccine against influenza and Alzheimer's disease
    Hayk Davtyan
    Department of Molecular Immunology, Institute for Molecular Medicine, Huntington Beach, CA 92647, USA
    J Transl Med 9:127. 2011
    ..We hypothesized that in elderly people with pre-existing memory Th cells specific to influenza this dual vaccine will simultaneously boost anti-influenza immunity and induce production of therapeutically active anti-Aβ antibodies...
  6. pmc Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals
    Margaret J Wortman
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, 700 W, Olney Road, Norfolk, VA 23507, USA
    BMC Mol Biol 11:81. 2010
    ..The distinct but related roles of Purα suggest a need for expression regulated differently depending on intracellular and external signals...
  7. pmc D471G mutation in LCMV-NP affects its ability to self-associate and results in a dominant negative effect in viral RNA synthesis
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York 14642, USA
    Viruses 4:2137-61. 2012
    ..Additionally, we also document the dominant negative effect of D471G mutation on viral infection, suggesting that NP self-association is an excellent target for the development of new antivirals against arenaviruses...
  8. pmc Arenavirus nucleoproteins prevent activation of nuclear factor kappa B
    W W Shanaka I Rodrigo
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 86:8185-97. 2012
    ..Altogether, our findings demonstrate that arenavirus infection inhibits NF-κB-dependent innate immune and inflammatory responses, possibly playing a key role in the pathogenesis and virulence of arenavirus...
  9. pmc Self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 86:3307-17. 2012
    ....
  10. pmc The C-terminal region of lymphocytic choriomeningitis virus nucleoprotein contains distinct and segregable functional domains involved in NP-Z interaction and counteraction of the type I interferon response
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Virol 85:13038-48. 2011
    ..In addition, this NP-Z interaction represents a potential target for the development of antiviral drugs to combat human-pathogenic arenaviruses...
  11. doi request reprint Canine influenza viruses with modified NS1 proteins for the development of live-attenuated vaccines
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, NY, USA
    Virology 500:1-10. 2017
    ..Immunogenicity and protection efficacy was better than that observed with an IIV. This is the first description of a LAIV for the prevention of H3N8 CIV in dogs...
  12. pmc Inhibition of arenavirus by A3, a pyrimidine biosynthesis inhibitor
    Emilio Ortiz-Riaño
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 88:878-89. 2014
    ..We document an additive antiarenavirus effect of A3 and ribavirin, supporting the potential combination therapy of ribavirin and pyrimidine biosynthesis inhibitors for the treatment of arenavirus infections. ..
  13. pmc Development and applications of single-cycle infectious influenza A virus (sciIAV)
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY, United States
    Virus Res 216:26-40. 2016
    ..Here, we review the recent burst in sciIAV generation and summarize the applications and findings on this important human pathogen using biocontained viral mimics. ..
  14. pmc Replication-competent fluorescent-expressing influenza B virus
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA
    Virus Res 213:69-81. 2016
    ..Lastly, fluorescent-expressing IBVs can be combined with the recently described reporter-expressing IAVs for the identification of novel therapeutics to combat these two important human respiratory pathogens. ..
  15. pmc Influenza A virus attenuation by codon deoptimization of the NS gene for vaccine development
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 88:10525-40. 2014
    ....
  16. pmc Influenza A and B virus intertypic reassortment through compatible viral packaging signals
    Steven F Baker
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 88:10778-91. 2014
    ....
  17. pmc Development of live-attenuated arenavirus vaccines based on codon deoptimization
    Benson Yee Hin Cheng
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 89:3523-33. 2015
    ..These results provide proof of concept of the safety, efficacy, and stability of a CD-based approach for developing live-attenuated vaccine candidates against human-pathogenic arenaviruses...
  18. pmc Mutations Designed by Ensemble Defect to Misfold Conserved RNA Structures of Influenza A Segments 7 and 8 Affect Splicing and Attenuate Viral Replication in Cell Culture
    Tian Jiang
    Department of Chemistry, University of Rochester, Rochester, New York, United States of America
    PLoS ONE 11:e0156906. 2016
    ..The results also demonstrate that the ensemble defect approach is an efficient way to test for function of RNA sequences. ..
  19. pmc Replication-Competent Influenza A Viruses Expressing Reporter Genes
    Michael Breen
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Viruses 8:. 2016
    ..In summary, reporter-expressing, replicating-competent IAV represent a powerful tool for the study of IAV both in vitro and in vivo. ..
  20. pmc NS1 Protein Mutation I64T Affects Interferon Responses and Virulence of Circulating H3N2 Human Influenza A Viruses
    Marta L DeDiego
    David H Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, New York, USA Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 90:9693-9711. 2016
    ..These data demonstrate the importance of influenza virus surveillance in identifying new mutations in the NS1 protein, affecting its ability to inhibit innate immune responses and, as a consequence, the pathogenicity of the virus...
  21. pmc Development of live-attenuated arenavirus vaccines based on codon deoptimization of the viral glycoprotein
    Benson Y H Cheng
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Virology 501:35-46. 2017
    ..Our findings support the feasibility of implementing an arenavirus GP CD-based approach for the development of safe and effective live-attenuated vaccines (LAVs) to combat diseases caused by human pathogenic arenaviruses...
  22. pmc Induction of CD8 T cell heterologous protection by a single dose of single-cycle infectious influenza virus
    Hailong Guo
    Center for Infectious Diseases and Vaccine Immunology, Rochester General Hospital Research Institute, Rochester, New York, USA
    J Virol 88:12006-16. 2014
    ..Without these cells, protection against lethal challenge was essentially lost. Our data indicate that an influenza vaccine that primarily relies on CD8 T cells for protection could be developed...
  23. pmc Protection against lethal influenza with a viral mimic
    Steven F Baker
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 87:8591-605. 2013
    ..All together, our studies suggest that sciIV may have potential as a broadly protective vaccine against influenza virus. ..
  24. pmc NS1 protein amino acid changes D189N and V194I affect interferon responses, thermosensitivity and virulence of circulating H3N2 human influenza A viruses
    Aitor Nogales
    David H Smith Center for Vaccine Biology and Immunology, and Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol . 2016
    ..These studies are relevant in order to identify new residues important for NS1 functions, and in human influenza virus surveillance to assess mutations affecting the pathogenicity of circulating viruses...
  25. pmc Competitive detection of influenza neutralizing antibodies using a novel bivalent fluorescence-based microneutralization assay (BiFMA)
    Steven F Baker
    Department of Microbiology and Immunology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, United States
    Vaccine 33:3562-70. 2015
    ..Importantly, this novel BiFMA can be used for any given influenza HA-pseudotyped virus under BSL-2 facilities, including highly pathogenic influenza HA isolates. ..
  26. pmc Replication-Competent Influenza A and B Viruses Expressing a Fluorescent Dynamic Timer Protein for In Vitro and In Vivo Studies
    Michael Breen
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York, 14642, United States of America
    PLoS ONE 11:e0147723. 2016
    ..By providing the ability to chronologically track viral spread, Timer-expressing influenza viruses are an excellent option to evaluate the in vitro and in vivo dynamics of viral infection. ..
  27. pmc Development of a mouse-adapted live attenuated influenza virus that permits in vivo analysis of enhancements to the safety of live attenuated influenza virus vaccine
    Andrew Cox
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 89:3421-6. 2015
    ..This model may permit preliminary safety analysis of improved LAIVs. ..
  28. pmc Arenavirus Genome Rearrangement for the Development of Live Attenuated Vaccines
    Benson Yee Hin Cheng
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 89:7373-84. 2015
    ..Attenuation of rLCMV/TransS was associated with reduced NP expression levels. These results open a new avenue for the development of arenavirus live attenuated vaccines based on rearrangement of their viral genome...
  29. pmc An improved method for estimating antibody titers in microneutralization assay using green fluorescent protein
    Hongmei Yang
    a Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, New York, USA
    J Biopharm Stat 26:409-20. 2016
    ..Our simulation study indicates that the new response approach outperforms the traditional response approach regardless of the data variability. The proposed new response approach can be used in similar assays for other disease models. ..
  30. pmc Temperature-Sensitive Live-Attenuated Canine Influenza Virus H3N8 Vaccine
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 91:. 2017
    ..These results demonstrate the feasibility of implementing a LAIV approach for the prevention and control of H3N8 CIV in dogs and suggest the need for a new LAIV for the control of H3N2 CIV...
  31. doi request reprint Human antibodies against dengue enhance dengue viral infectivity without suppressing type I interferon secretion in primary human monocytes
    Zhihua Kou
    Department of Medicine, University of Rochester, Rochester, NY 14642, USA
    Virology 410:240-7. 2011
    ..These results demonstrate that the regulation of virus production in ADE infection of primary human monocytes is more complex than previously appreciated...
  32. pmc Rearrangement of Influenza Virus Spliced Segments for the Development of Live-Attenuated Vaccines
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA
    J Virol 90:6291-302. 2016
    ....
  33. pmc Replication-competent influenza A viruses expressing a red fluorescent protein
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, NY, United States
    Virology 476:206-16. 2015
    ....
  34. pmc The specialized proresolving mediator 17-HDHA enhances the antibody-mediated immune response against influenza virus: a new class of adjuvant?
    Sesquile Ramon
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642 Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115 and
    J Immunol 193:6031-40. 2014
    ..Furthermore, this work has important implications for the understanding of B cell biology, as well as the development of new potential vaccine adjuvants. ..
  35. pmc Use of single-cycle infectious lymphocytic choriomeningitis virus to study hemorrhagic fever arenaviruses
    W W Shanaka I Rodrigo
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Virol 85:1684-95. 2011
    ..Using GP-pseudotyped rLCMVΔGP/GFP, we have also obtained evidence supporting the feasibility of this approach to identify and evaluate candidate antiviral drugs against HF arenaviruses without the need of BSL-4 laboratories...
  36. pmc Novel strategies for development of hemorrhagic fever arenavirus live-attenuated vaccines
    Luis Martinez-Sobrido
    a Department of Microbiology and Immunology, University of Rochester Medical Center Ringgold standard institution, Rochester, NY, USA
    Expert Rev Vaccines 15:1113-21. 2016
    ..Moreover, HF arenaviruses represent credible biodefense threats. There are not FDA-approved arenavirus vaccines and current anti-arenaviral therapy is limited to an off-label use of ribavirin that is only partially effective...
  37. pmc Antisense Oligonucleotides Targeting Influenza A Segment 8 Genomic RNA Inhibit Viral Replication
    Elzbieta Lenartowicz
    1 Department of Chemistry, University of Rochester, Rochester, New York
    Nucleic Acid Ther 26:277-285. 2016
    ..These studies establish the accessibility of IAV vRNA for ASOs in regions other than the panhandle formed between the 5' and 3' ends. Thus, these regions can provide targets for the development of novel IAV antiviral approaches...
  38. ncbi request reprint Mucosal immunization with attenuated Shigella flexneri harboring an influenza hemagglutinin DNA vaccine protects mice against a lethal influenza challenge
    William H Vecino
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Virology 325:192-9. 2004
    ..These results suggest that S. flexneri Deltaasd strain 15D is a promising vector for mucosal DNA vaccine immunization against influenza virus and other mucosal pathogens...
  39. pmc Use of bacteriophage particles displaying influenza virus hemagglutinin for the detection of hemagglutination-inhibition antibodies
    William Domm
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester 14642, NY, USA
    J Virol Methods 197:47-50. 2014
    ..These results demonstrate the utility of HA-displaying bacteriophage capsids for the detection of influenza virus-specific HAI antibodies. ..
  40. pmc Reverse Genetics Approaches for the Development of Influenza Vaccines
    Aitor Nogales
    Department of Microbiology and Immunology, University of Rochester, Rochester, NY 14642, USA
    Int J Mol Sci 18:. 2016
    ....
  41. pmc The human cytomegalovirus UL26 protein antagonizes NF-κB activation
    Chun Mathers
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York, USA
    J Virol 88:14289-300. 2014
    ..Combined, our results indicate that U(L)26 is part of a viral program to antagonize innate immunity through modulation of NF-κB signaling...