Alexander M Makhov

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Visualization of the annealing of complementary single-stranded DNA catalyzed by the herpes simplex virus type 1 ICP8 SSB/recombinase
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 355:911-22. 2006
  2. ncbi request reprint Two-dimensional crystallization of herpes simplex virus type 1 single-stranded DNA-binding protein, ICP8, on a lipid monolayer
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Campus Box 7295, Chapel Hill, NC 27599 7295, USA
    Biochim Biophys Acta 1701:101-8. 2004
  3. pmc Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia
    Liang Cai
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Cell 134:828-42. 2008
  4. ncbi request reprint The Epstein-Barr virus polymerase accessory factor BMRF1 adopts a ring-shaped structure as visualized by electron microscopy
    Alexander M Makhov
    Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 279:40358-61. 2004
  5. pmc Origin-specific unwinding of herpes simplex virus 1 DNA by the viral UL9 and ICP8 proteins: visualization of a specific preunwinding complex
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Proc Natl Acad Sci U S A 100:898-903. 2003
  6. pmc The bipolar filaments formed by herpes simplex virus type 1 SSB/recombination protein (ICP8) suggest a mechanism for DNA annealing
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, Department Microbiology and Immunology, University of North Carolina at Chapel Hill, 27517, USA
    J Mol Biol 386:273-9. 2009
  7. ncbi request reprint Architecture of the replication complex and DNA loops at the fork generated by the bacteriophage t4 proteins
    Paul D Chastain
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:21276-85. 2003
  8. pmc Details of ssDNA annealing revealed by an HSV-1 ICP8-ssDNA binary complex
    Gökhan Tolun
    Lineberger Comprehensive Cancer Center, and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 41:5927-37. 2013
  9. ncbi request reprint Hairpin formation in Friedreich's ataxia triplet repeat expansion
    Brooke L Heidenfelder
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, 27599 7295, USA
    J Biol Chem 278:2425-31. 2003
  10. ncbi request reprint F-actin binding is essential for coronin 1B function in vivo
    Liang Cai
    Lineberger Comprehensive Cancer Center, Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    J Cell Sci 120:1779-90. 2007

Collaborators

Detail Information

Publications17

  1. ncbi request reprint Visualization of the annealing of complementary single-stranded DNA catalyzed by the herpes simplex virus type 1 ICP8 SSB/recombinase
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Mol Biol 355:911-22. 2006
    ..Mechanisms for how the coiled-coil structures facilitate annealing are discussed...
  2. ncbi request reprint Two-dimensional crystallization of herpes simplex virus type 1 single-stranded DNA-binding protein, ICP8, on a lipid monolayer
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Campus Box 7295, Chapel Hill, NC 27599 7295, USA
    Biochim Biophys Acta 1701:101-8. 2004
    ..5 nm filament with a shallow 31.2 nm pitch were observed and reconstruction revealed a double-helical structure...
  3. pmc Coronin 1B antagonizes cortactin and remodels Arp2/3-containing actin branches in lamellipodia
    Liang Cai
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Cell 134:828-42. 2008
    ..We conclude that Coronin 1B replaces the Arp2/3 complex at actin filament branches as the dendritic network matures and drives the turnover of branched actin networks...
  4. ncbi request reprint The Epstein-Barr virus polymerase accessory factor BMRF1 adopts a ring-shaped structure as visualized by electron microscopy
    Alexander M Makhov
    Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 279:40358-61. 2004
    ..These findings extend the theme of polymerase accessory factors adopting ring-shaped structures and provide an example in which the ring is significantly larger than any previously described sliding clamp...
  5. pmc Origin-specific unwinding of herpes simplex virus 1 DNA by the viral UL9 and ICP8 proteins: visualization of a specific preunwinding complex
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Proc Natl Acad Sci U S A 100:898-903. 2003
    ..Unwinding proceeded until the acquisition of a high level of positive supertwists in the remaining duplex DNA inhibited further unwinding. Addition of topoisomerase I allowed further unwinding, opening >1 kb of DNA around oriS...
  6. pmc The bipolar filaments formed by herpes simplex virus type 1 SSB/recombination protein (ICP8) suggest a mechanism for DNA annealing
    Alexander M Makhov
    Lineberger Comprehensive Cancer Center, Department Microbiology and Immunology, University of North Carolina at Chapel Hill, 27517, USA
    J Mol Biol 386:273-9. 2009
    ....
  7. ncbi request reprint Architecture of the replication complex and DNA loops at the fork generated by the bacteriophage t4 proteins
    Paul D Chastain
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:21276-85. 2003
    ..These bobbins constitute a major portion of the mass of the full replication complex...
  8. pmc Details of ssDNA annealing revealed by an HSV-1 ICP8-ssDNA binary complex
    Gökhan Tolun
    Lineberger Comprehensive Cancer Center, and Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 41:5927-37. 2013
    ..3 MDa. Fitting of the ICP8 crystal structure suggests a mechanism for the annealing reaction catalyzed by ICP8, which is most likely a general mechanism for protein-driven DNA annealing...
  9. ncbi request reprint Hairpin formation in Friedreich's ataxia triplet repeat expansion
    Brooke L Heidenfelder
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, 27599 7295, USA
    J Biol Chem 278:2425-31. 2003
    ..The results provide unifying evidence that predicts that hairpin formation during DNA synthesis mediates all of the disease-associated, triplet repeat expansions...
  10. ncbi request reprint F-actin binding is essential for coronin 1B function in vivo
    Liang Cai
    Lineberger Comprehensive Cancer Center, Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    J Cell Sci 120:1779-90. 2007
    ..Furthermore, our analysis indicates that F-actin binding is absolutely required for coronin 1B to exert its effects on whole-cell motility and lamellipodial dynamics...
  11. pmc Unpacking a gel-forming mucin: a view of MUC5B organization after granular release
    Mehmet Kesimer
    1Department of Biochemistry and Biophysics and Cystic Fibrosis Pulmonary Research and Treatment Center, Chapel Hill, NC, USA
    Am J Physiol Lung Cell Mol Physiol 298:L15-22. 2010
    ....
  12. ncbi request reprint Regulation of the interaction of inosine monophosphate dehydrogenase with mycophenolic Acid by GTP
    YanShan Ji
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 281:206-12. 2006
    ..We conclude that intracellular GTP acts as an antagonist to MPA by directly binding to IMPDH and reversing the conformational changes in the protein...
  13. pmc Preferential binding of ATR protein to UV-damaged DNA
    Keziban Unsal-Kaçmaz
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 99:6673-8. 2002
    ..In addition, damaged DNA stimulates the kinase activity of ATR to a significantly higher level than undamaged DNA. Our data suggest that ATR may function as an initial sensor in the DNA damage checkpoint response...
  14. ncbi request reprint Structural features of the Bluetongue virus NS2 protein
    Eleni Mumtsidu
    European Molecular Biology Laboratory, Hamburg Outstation, c o DESY, Notkestrasse 85, D22603 Hamburg, Germany
    J Struct Biol 160:157-67. 2007
    ..This decamer changes conformation upon binding of a non-hydrolysable ATP analogue...
  15. pmc Chaperone proteins abrogate inhibition of the human papillomavirus (HPV) E1 replicative helicase by the HPV E2 protein
    Biing Yuan Lin
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 35294 0005, USA
    Mol Cell Biol 22:6592-604. 2002
    ..An E1 mutation in the ATP binding site caused deficient binding and unwinding of origin DNA, indicating the importance of ATP binding in efficient helicase assembly on the origin...
  16. ncbi request reprint Architecture of the bacteriophage T4 replication complex revealed with nanoscale biopointers
    Nancy G Nossal
    Laboratory of Molecular and Cellular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
    J Biol Chem 282:1098-108. 2007
    ..Unexpectedly, we found that 59 helicase-loading protein remains on the fork after loading the helicase and is present on molecules with extensive replication...
  17. ncbi request reprint Amplification of telomeric arrays via rolling-circle mechanism
    Jozef Nosek
    Department of Biochemistry, Mlynska dolina CH 1, Comenius University, 842 15 Bratislava, Slovakia
    J Biol Chem 280:10840-5. 2005
    ....