Kazuya Machida

Summary

Affiliation: University of Connecticut Health Center
Country: USA

Publications

  1. ncbi request reprint The SH2 domain: versatile signaling module and pharmaceutical target
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Biochim Biophys Acta 1747:1-25. 2005
  2. doi request reprint Detection of protein-protein interactions by far-western blotting
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA
    Methods Mol Biol 536:313-29. 2009
  3. pmc Fast rebinding increases dwell time of Src homology 2 (SH2)-containing proteins near the plasma membrane
    Dongmyung Oh
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 109:14024-9. 2012
  4. ncbi request reprint High-throughput phosphotyrosine profiling using SH2 domains
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Cell 26:899-915. 2007
  5. pmc The application of modular protein domains in proteomics
    Joshua A Jadwin
    Department of Genetics and Developmental Biology, Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06030, USA
    FEBS Lett 586:2586-96. 2012
  6. ncbi request reprint Profiling the global tyrosine phosphorylation state
    Kazuya Machida
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Mol Cell Proteomics 2:215-33. 2003
  7. pmc Characterizing tyrosine phosphorylation signaling in lung cancer using SH2 profiling
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS ONE 5:e13470. 2010
  8. doi request reprint Detection and quantification of protein-protein interactions by far-western blotting
    Joshua A Jadwin
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06030, USA
    Methods Mol Biol 1312:379-98. 2015
  9. pmc SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
    Christopher M Thompson
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, 06030, Farmington, CT, USA
    BMC Biotechnol 15:60. 2015
  10. doi request reprint Expression and Purification of SH2 Domains Using Baculovirus Expression System
    Mari Ogiue-Ikeda
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06032, USA
    Methods Mol Biol 1555:183-198. 2017

Collaborators

Detail Information

Publications15

  1. ncbi request reprint The SH2 domain: versatile signaling module and pharmaceutical target
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Biochim Biophys Acta 1747:1-25. 2005
    ..These studies have also led to new insights into molecular recognition by the SH2 domain...
  2. doi request reprint Detection of protein-protein interactions by far-western blotting
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA
    Methods Mol Biol 536:313-29. 2009
    ..We here describe a rapid and simple protocol for far-western blotting, in which GST-tagged Src homology 2 (SH2) domains are used to probe cellular proteins in a phosphorylation-dependent manner...
  3. pmc Fast rebinding increases dwell time of Src homology 2 (SH2)-containing proteins near the plasma membrane
    Dongmyung Oh
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 109:14024-9. 2012
    ....
  4. ncbi request reprint High-throughput phosphotyrosine profiling using SH2 domains
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
    Mol Cell 26:899-915. 2007
    ..These results demonstrate that high-throughput comprehensive SH2 profiling provides valuable mechanistic insights into tyrosine kinase signaling pathways...
  5. pmc The application of modular protein domains in proteomics
    Joshua A Jadwin
    Department of Genetics and Developmental Biology, Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06030, USA
    FEBS Lett 586:2586-96. 2012
    ....
  6. ncbi request reprint Profiling the global tyrosine phosphorylation state
    Kazuya Machida
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Mol Cell Proteomics 2:215-33. 2003
    ..We also describe ongoing efforts to modify and improve SH2 profiling, including the development of a multiplexed assay system that will allow high-throughput functional profiling of the tyrosine phosphoproteome...
  7. pmc Characterizing tyrosine phosphorylation signaling in lung cancer using SH2 profiling
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut, United States of America
    PLoS ONE 5:e13470. 2010
    ..However, the comprehensive analysis of tyrosine phosphorylation of large numbers of human cancer specimens is technically challenging using current methods...
  8. doi request reprint Detection and quantification of protein-protein interactions by far-western blotting
    Joshua A Jadwin
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06030, USA
    Methods Mol Biol 1312:379-98. 2015
    ..We also present a batch quantification method that allows for the direct comparison of probe binding patterns. ..
  9. pmc SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
    Christopher M Thompson
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, 06030, Farmington, CT, USA
    BMC Biotechnol 15:60. 2015
    ..However, in order to use this method to investigate SH2 binding sites on a specific target in cell lysate, additional procedures such as pull-down or immunoprecipitation which consume large amounts of sample are required...
  10. doi request reprint Expression and Purification of SH2 Domains Using Baculovirus Expression System
    Mari Ogiue-Ikeda
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06032, USA
    Methods Mol Biol 1555:183-198. 2017
    ..We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system...
  11. doi request reprint Functionally Altered SH2 Domains for Biochemical Studies: Loss-of-Function Mutant and Domain Concatenation
    Mari Ogiue-Ikeda
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06030, USA
    Methods Mol Biol 1555:199-223. 2017
    ..In this chapter, we describe preparation of loss-of-function and concatenated (tandem) SH2 domains that should be widely applicable to both high- and low-throughput phosphoproteomics studies...
  12. doi request reprint Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull-Down, and Fluorescence Polarization
    Kazuya Machida
    Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Avenue, Farmington, CT, 06030, USA
    Methods Mol Biol 1555:307-330. 2017
    ..Here, we outline standard protocols for these established assays using recombinant SH2 domain, emphasizing the importance of appropriate sample preparation and assay controls...
  13. pmc Deciphering Phosphotyrosine-Dependent Signaling Networks in Cancer by SH2 Profiling
    Kazuya Machida
    Raymond and Beverly Sacker Laboratory of Genetics and Molecular Medicine, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA
    Genes Cancer 3:353-61. 2012
    ..SH2 profiling can serve as an alternative phosphoproteomics tool to dissect aberrant tyrosine kinase pathways responsible for individual malignancies, with the goal of facilitating personalized diagnostics for the treatment of cancer...
  14. ncbi request reprint Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domains
    Kevin Dierck
    Department of Clinical Chemistry, Center of Clinical Pathology, University Medical Center Hamburg Eppendorf, Martinistr 52, 20246 Hamburg, Germany
    Nat Methods 3:737-44. 2006
    ....
  15. ncbi request reprint Tyrosine phosphorylation of NOS3 in a breast cancer cell line and Src-transformed cells
    Yasushi Takenouchi
    Division of Cancer Biology, Nagoya University Graduate School of Medicine, Showa Ku, Nagoya 466 8550, Japan
    Oncol Rep 11:1059-62. 2004
    ..Taken together, our results suggest that active, membrane-bound form of Src can induce constitutive phosphorylation of NOS3...