C Y Liu

Summary

Affiliation: University of Cincinnati
Country: USA

Publications

  1. pmc Preservation and expansion of the primate keratocyte phenotype by downregulating TGF-beta signaling in a low-calcium, serum-free medium
    Tetsuya Kawakita
    TissueTech, Inc, Ocular Surface Center, Miami, Florida 33176, USA
    Invest Ophthalmol Vis Sci 47:1918-27. 2006
  2. ncbi request reprint Identification of a 3.2 kb 5'-flanking region of the murine keratocan gene that directs beta-galactosidase expression in the adult corneal stroma of transgenic mice
    C Liu
    Department of Ophthalmology, College of Medicine, University of Cincinnati, OH 45267 0527, USA
    Gene 250:85-96. 2000
  3. ncbi request reprint The cloning of mouse keratocan cDNA and genomic DNA and the characterization of its expression during eye development
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 273:22584-8. 1998
  4. ncbi request reprint TGFbeta2 in corneal morphogenesis during mouse embryonic development
    S Saika
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio, USA
    Dev Biol 240:419-32. 2001
  5. ncbi request reprint MK/T-1, an immortalized fibroblast cell line derived using cultures of mouse corneal stroma
    R L Gendron
    Department of Pediatrics, Division of Hematology and Oncology, Children s Hospital Medical Center, University of Cincinnati, Cincinnati, OH 45229 3039, USA
    Mol Vis 7:107-13. 2001
  6. ncbi request reprint Cornea-specific expression of K12 keratin during mouse development
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, OH 45267 0527
    Curr Eye Res 12:963-74. 1993
  7. pmc Role of lumican in the corneal epithelium during wound healing
    S Saika
    Department of Ophthalmology, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0527, USA
    J Biol Chem 275:2607-12. 2000
  8. ncbi request reprint Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Ohio 45267 0527
    J Biol Chem 269:24627-36. 1994
  9. ncbi request reprint Developmental patterns of two alpha 1(IX) collagen mRNA isoforms in mouse
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Ohio 45267 0527
    Dev Dyn 198:150-7. 1993
  10. pmc The heterogeneous murine corneal stromal cell populations in vitro
    Edgar M Espana
    TissueTech, Inc and Ocular Surface Center, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 46:4528-35. 2005

Collaborators

Detail Information

Publications46

  1. pmc Preservation and expansion of the primate keratocyte phenotype by downregulating TGF-beta signaling in a low-calcium, serum-free medium
    Tetsuya Kawakita
    TissueTech, Inc, Ocular Surface Center, Miami, Florida 33176, USA
    Invest Ophthalmol Vis Sci 47:1918-27. 2006
    ..To demonstrate whether the original keratocyte phenotype is maintained with proliferative activity by suppressing TGF-beta signaling in rhesus monkey keratocytes expanded in a serum-free and low-[Ca2+] medium...
  2. ncbi request reprint Identification of a 3.2 kb 5'-flanking region of the murine keratocan gene that directs beta-galactosidase expression in the adult corneal stroma of transgenic mice
    C Liu
    Department of Ophthalmology, College of Medicine, University of Cincinnati, OH 45267 0527, USA
    Gene 250:85-96. 2000
    ....
  3. ncbi request reprint The cloning of mouse keratocan cDNA and genomic DNA and the characterization of its expression during eye development
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 273:22584-8. 1998
    ..5 approximately E18.5. Interestingly, keratocan mRNA can be detected in scleral cells of E15.5 embryos, but not in E18.5 embryos. In adult eyes, keratocan mRNA can be detected in corneal keratocytes, but not in scleral cells...
  4. ncbi request reprint TGFbeta2 in corneal morphogenesis during mouse embryonic development
    S Saika
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio, USA
    Dev Biol 240:419-32. 2001
    ..Delayed appearance of macrophages in ocular tissues was observed in Tgfb2(-/-) mice. Malfunctioning macrophages may account for accumulation of cell mass in vitreous of Tgfb2 null mice...
  5. ncbi request reprint MK/T-1, an immortalized fibroblast cell line derived using cultures of mouse corneal stroma
    R L Gendron
    Department of Pediatrics, Division of Hematology and Oncology, Children s Hospital Medical Center, University of Cincinnati, Cincinnati, OH 45229 3039, USA
    Mol Vis 7:107-13. 2001
    ..Immortalized cell lines representing fibroblast cells from corneal stroma would facilitate studies of corneal cell biology and injury response...
  6. ncbi request reprint Cornea-specific expression of K12 keratin during mouse development
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, OH 45267 0527
    Curr Eye Res 12:963-74. 1993
    ..Taken together, our results demonstrate that the expression of K12 keratin is cornea-specific, differentiation-dependent, and developmentally regulated...
  7. pmc Role of lumican in the corneal epithelium during wound healing
    S Saika
    Department of Ophthalmology, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0527, USA
    J Biol Chem 275:2607-12. 2000
    ..These observations indicate that lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing...
  8. ncbi request reprint Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Ohio 45267 0527
    J Biol Chem 269:24627-36. 1994
    ..It is not known whether these alternate K12 poly(A)+ RNAs have any biological functions, e.g. regulation of K12 gene expression...
  9. ncbi request reprint Developmental patterns of two alpha 1(IX) collagen mRNA isoforms in mouse
    C Y Liu
    Department of Ophthalmology, University of Cincinnati, Ohio 45267 0527
    Dev Dyn 198:150-7. 1993
    ..This differs from that of chick cornea development. In summary, the expression of the Col9a1 gene shows a temporospatial pattern throughout mouse eye development.(ABSTRACT TRUNCATED AT 250 WORDS)..
  10. pmc The heterogeneous murine corneal stromal cell populations in vitro
    Edgar M Espana
    TissueTech, Inc and Ocular Surface Center, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 46:4528-35. 2005
    ..To demonstrate that the murine corneal stroma is inhabited by heterogeneous cell populations that include cells expressing nestin...
  11. pmc Focus on molecules: lumican
    Winston W Y Kao
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH, USA
    Exp Eye Res 82:3-4. 2006
  12. ncbi request reprint Characterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice
    Tai ichiro Chikama
    Department of Ophthahlmology, University of Cincinnati, Ohio 45267, USA
    Invest Ophthalmol Vis Sci 46:1966-72. 2005
    ..To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline...
  13. pmc Keratocan, a cornea-specific keratan sulfate proteoglycan, is regulated by lumican
    Eric C Carlson
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio 45267 0527, USA
    J Biol Chem 280:25541-7. 2005
    ..The results also suggest a critical level of small proteoglycans to be essential for collagen organization but that overabundance is not detrimental to extracellular matrix morphogenesis...
  14. ncbi request reprint Over expression of FGF7 enhances cell proliferation but fails to cause pathology in corneal epithelium of Kerapr-rtTA/FGF7 bitransgenic mice
    Miyuki Hayashi
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH 45267 0527, USA
    Mol Vis 11:201-7. 2005
    ..In the present study, we used a binary inducible transgenic mouse model to examine the role of FGF7 on corneal epithelium proliferation...
  15. pmc Soluble lumican glycoprotein purified from human amniotic membrane promotes corneal epithelial wound healing
    Lung Kun Yeh
    Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, Florida 33136, USA
    Invest Ophthalmol Vis Sci 46:479-86. 2005
    ..To purify and characterize the glycoprotein lumican, isolated from human amniotic membrane (AM), and to examine its efficacy in treating corneal epithelium debridement...
  16. ncbi request reprint Pax6 overexpression suppresses cell proliferation and retards the cell cycle in corneal epithelial cells
    Jie Ouyang
    Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, FL 33136, USA, and Department of Ophthalmology, Veterans General Hospital, Taichung, Taiwan
    Invest Ophthalmol Vis Sci 47:2397-407. 2006
    ..Pax6 continues to be expressed in the ocular surface epithelia of the postnatal eye. The goal of this study was to investigate a possible role for Pax6 in controlling dynamics of the ocular surface epithelia...
  17. ncbi request reprint Eye drop delivery of nano-polymeric micelle formulated genes with cornea-specific promoters
    Yaw Chong Tong
    College of Pharmacy, Taipei Medical University, Taipei, Taiwan
    J Gene Med 9:956-66. 2007
    ..keratin 12 (K12) and keratocan (Kera3.2) promoters, by non-ionic poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) polymeric micelles (PM) to mouse and rabbit eyes, and investigates the underlying mechanisms...
  18. pmc Keratocan and lumican regulate neutrophil infiltration and corneal clarity in lipopolysaccharide-induced keratitis by direct interaction with CXCL1
    Eric C Carlson
    Department of Ophthalmology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 282:35502-9. 2007
    ..Together, these data support a significant role for lumican and keratocan in a corneal inflammatory response with respect to edema, corneal clarity, and cellular infiltration...
  19. ncbi request reprint Molecular analysis and characterization of zebrafish keratocan (zKera) gene
    Lung Kun Yeh
    Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    J Biol Chem 283:506-17. 2008
    ..This is different from mouse, for which keratocan-deficient mice are viable. Taken together, our data indicate that mammalian keratocan is conserved in zebrafish in terms of gene structure, expression pattern, and promoter function...
  20. ncbi request reprint Targeted expression of a lumican transgene rescues corneal deficiencies in lumican-null mice
    Johanna T A Meij
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH, USA
    Mol Vis 13:2012-8. 2007
    ..To investigate whether targeted expression of lumican in the mouse cornea rescued the Lum(-/-) phenotype...
  21. doi request reprint Morphological differences between the trabecular meshworks of zebrafish and mammals
    Chun Chen Chen
    Department of Ophthalmology, Taipei City Hospital, Ren Ai Branch, Taipei, Taiwan
    Curr Eye Res 33:59-72. 2008
    ..This study investigated and compared the histological morphologies and compositions of the extracellular matrices of the TMs of the zebrafish and some commonly used animal models, including the mouse, rat, rabbit, and cow...
  22. ncbi request reprint Signaling pathways in morphogenesis of cornea and eyelid
    Winston W Y Kao
    Department of Ophthalmology, University of Cincinnati, Cincinnati, Ohio 45267 0838, USA
    Ocul Surf 6:9-23. 2008
    ..This review discusses known signaling transduction pathways involved in the morphogenesis of the cornea and eyelids during embryonic development and homeostasis in adults, using genetically modified experimental animal models...
  23. pmc Excess FGF-7 in corneal epithelium causes corneal intraepithelial neoplasia in young mice and epithelium hyperplasia in adult mice
    Taiichiro Chikama
    Department of Ophthalmology, University of Cincinnati Medical Center, 3223 Eden Ave, Suite 350, Cincinnati, OH 45267 0527, USA
    Am J Pathol 172:638-49. 2008
    ..Taken together, Krt12-rtTA/tet-O-FGF-7 mice may be a suitable animal model for the study of the molecular and cellular mechanisms of human OSSN...
  24. pmc Promiscuous recombination of LoxP alleles during gametogenesis in cornea Cre driver mice
    Daniel Y Weng
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH 45267 0838, USA
    Mol Vis 14:562-71. 2008
    ..To examine whether promiscuous Cre/LoxP recombination happens during gametogenesis in double transgenic mice carrying LoxP modified alleles and Cre transgene driven by tissue-specific promoter outside the gonads of adult mice...
  25. pmc PITX2 gain-of-function in Rieger syndrome eye model
    Johan Holmberg
    Lund University, Department of Cell and Molecular Biology, Section for Cell and Developmental Biology, BMC B12, Tornavagen 10, SE 22184 Lund, Sweden
    Am J Pathol 165:1633-41. 2004
    ..These findings indicate a novel pathogenetic mechanism in which excess corneal and iridal PITX2A cause glaucoma and anterior defects that closely resemble Axenfeld-Rieger syndrome...
  26. ncbi request reprint Cis-regulatory elements of the mouse Krt1.12 gene
    I Jong Wang
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH, USA
    Mol Vis 8:94-101. 2002
    ..To better understand the regulatory mechanism of its expression, the cis-regulatory elements located between the transcription start site and 600 bp upstream of the Krt1.12 gene were determined...
  27. ncbi request reprint Stromal niche controls the plasticity of limbal and corneal epithelial differentiation in a rabbit model of recombined tissue
    Edgar M Espana
    TissueTech, Inc, and Ocular Surface Research and Education Foundation, 7000 SW 97 Avenue, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 44:5130-5. 2003
    ..This study was conducted to determine whether the limbal stroma dictates the plasticity of limbal and corneal epithelial differentiation...
  28. ncbi request reprint Roles of lumican and keratocan on corneal transparency
    Winston W Y Kao
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH 45267 0527, USA
    Glycoconj J 19:275-85. 2002
    ....
  29. pmc A role for MEK kinase 1 in TGF-beta/activin-induced epithelium movement and embryonic eyelid closure
    Lin Zhang
    Department of Environmental Health, University of Cincinnati Medical Center, Cincinnati, OH 45267, USA
    EMBO J 22:4443-54. 2003
    ..This study also suggests that the signaling mechanisms that control eyelid closure in mammals and dorsal closure in Drosophila are evolutionarily conserved...
  30. ncbi request reprint Response of lens epithelial cells to injury: role of lumican in epithelial-mesenchymal transition
    Shizuya Saika
    Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan
    Invest Ophthalmol Vis Sci 44:2094-102. 2003
    ....
  31. ncbi request reprint Keratocan-deficient mice display alterations in corneal structure
    Chia Yang Liu
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, McKnight Vision Research Center, 1638 NW 10th Avenue, Rm 621, Miami, FL 33136, USA
    J Biol Chem 278:21672-7. 2003
    ..Keratocan thus plays a unique role in maintaining the appropriate corneal shape to ensure normal vision...
  32. pmc Role of Cys41 in the N-terminal domain of lumican in ex vivo collagen fibrillogenesis by cultured corneal stromal cells
    Eric C Carlson
    Department of Ophthalmology, University of Cincinnati, 3223 Eden Avenue, Cincinnati, OH 45267, USA
    Biochem J 369:461-8. 2003
    ..The results indicate that the cysteine-rich domain of lumican is important in collagen fibrillogenesis and stromal matrix assembly...
  33. ncbi request reprint Altered collagen fibril formation in the sclera of lumican-deficient mice
    Bobbie A Austin
    Department of Anatomy and Cell Biology, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    Invest Ophthalmol Vis Sci 43:1695-701. 2002
    ..In addition, lumican was characterized in the sclera of wild-type and lumican-deficient mice by Western blot analyses...
  34. ncbi request reprint Epithelial repair: roles of extracellular matrix
    Shizuya Saika
    Department of Ophthalmology, Wakayama Medical College, Japan
    Cornea 21:S23-9. 2002
    ..3) Expression of keratan sulfate proteoglycan (KSPG) proteins in corneal epithelium was examined during repair after injury in wild-type and lumican-null mice...
  35. pmc Excess biglycan causes eyelid malformation by perturbing muscle development and TGF-alpha signaling
    Yasuhito Hayashi
    Department of Ophthalmology, University of Cincinnati Medical Center, Cincinnati, OH 45267 0527, United States
    Dev Biol 277:222-34. 2005
    ....
  36. ncbi request reprint Altered KSPG expression by keratocytes following corneal injury
    Eric C Carlson
    Department of Ophthalmology, University of Cincinnati, Cincinnati, OH, USA
    Mol Vis 9:615-23. 2003
    ....
  37. ncbi request reprint Fibroblast growth factor 2: roles of regulation of lens cell proliferation and epithelial-mesenchymal transition in response to injury
    Takeshi Tanaka
    Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan
    Mol Vis 10:462-7. 2004
    ..To examine the role of fibroblast growth factor 2 (FGF2) in regulating lens cell proliferation and epithelial-mesenchymal transition (EMT) in response to injury...
  38. ncbi request reprint In vivo gene delivery and visualization of corneal stromal cells using an adenoviral vector and keratocyte-specific promoter
    Eric C Carlson
    Ophthalmic Research, Cole Eye Institute, The Cleveland Clinic Foundation, Ohio 44195, USA
    Invest Ophthalmol Vis Sci 45:2194-200. 2004
    ....
  39. pmc Smad3 signaling is required for epithelial-mesenchymal transition of lens epithelium after injury
    Shizuya Saika
    Department of Ophthalmology, Wakayama Medical University, Kimiidera, Wakayama, Japan
    Am J Pathol 164:651-63. 2004
    ..The results suggest that blocking the Smad3 pathway might be beneficial in inhibiting capsular fibrosis after injury and/or surgery...
  40. pmc Keratocan expression of murine keratocytes is maintained on amniotic membrane by down-regulating transforming growth factor-beta signaling
    Tetsuya Kawakita
    TissueTech, Inc and Ocular Surface Center, Miami, Florida 33173, USA
    J Biol Chem 280:27085-92. 2005
    ..This strategy of suppressing TGF-beta signaling, achieved by AM stromal matrix in part via suppression of TGF-beta gene transcription, can be used to expand keratocytes in culture without the use of AM in the future...
  41. ncbi request reprint Role of p38 MAP kinase in regulation of cell migration and proliferation in healing corneal epithelium
    Shizuya Saika
    Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan
    Invest Ophthalmol Vis Sci 45:100-9. 2004
    ..e., Smad and p38 mitogen-activated kinase [MAPK]) in regulation of cell migration and proliferation of healing mouse corneal epithelium...
  42. ncbi request reprint Human keratocytes cultured on amniotic membrane stroma preserve morphology and express keratocan
    Edgar M Espana
    TissueTech, Inc, and Ocular Surface Research and Education Foundation, 7000 SW 97 Avenue, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 44:5136-41. 2003
    ..To develop a new method of expanding human corneal keratocytes in serum while maintaining their characteristic morphology and keratocan expression...
  43. ncbi request reprint Cell lineage-specific effects associated with multiple deficiencies of tumor susceptibility genes in Msh2(-/-)Rb(+/-) mice
    Alexander Yu Nikitin
    Department of Molecular Medicine and Institute of Biotechnology, The University of Texas Health Science Center, San Antonio, TX 78245 3207, USA
    Cancer Res 62:5134-8. 2002
    ..Taken together, these data not only revealed a novel biological interaction between Rb and Msh2 but also cell lineage specificity effects associated with multiple deficiencies in these tumor susceptibility genes...
  44. ncbi request reprint Characterization of corneal pannus removed from patients with total limbal stem cell deficiency
    Edgar M Espana
    Ocular Surface Center, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 45:2961-6. 2004
    ..To determine the epithelial lineage of origin in corneal pannus tissue surgically removed from patients with total limbal stem cell (SC) deficiency...
  45. ncbi request reprint Calcium-induced abnormal epidermal-like differentiation in cultures of mouse corneal-limbal epithelial cells
    Tetsuya Kawakita
    TissueTech, Inc, Miami, Florida, USA
    Invest Ophthalmol Vis Sci 45:3507-12. 2004
    ..To develop a reproducible method for expanding mouse corneal-limbal epithelial cells and determine the role of extracellular Ca(2+) concentration and serum in modulating their growth and differentiation...
  46. ncbi request reprint CD-34 expression by cultured human keratocytes is downregulated during myofibroblast differentiation induced by TGF-beta1
    Edgar M Espana
    TissueTech, Inc, Miami, Florida 33173, USA
    Invest Ophthalmol Vis Sci 45:2985-91. 2004
    ..To establish CD34 as a cell surface marker for human keratocytes and to demonstrate its downregulation during TGF-beta1-induced myofibroblast differentiation...