Genomes and Genes
Affiliation: University of California
- Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetesBjorn Tyrberg
UCSD Cancer Center, La Jolla, CA 92037 0912, USA
BMC Endocr Disord 2:2. 2002..An increase of Ogg1 in islet cell mitochondria is consistent with a model in which hyperglycemia and consequent increased beta-cell oxidative metabolism lead to DNA damage and the induction of Ogg1 expression...
- Towards gene therapy of diabetes mellitusF Levine
Department of Pediatrics, Center for Molecular Genetics, UCSD School of Medicine, La Jolla, CA 92093 0634, USA
Mol Med Today 5:165-71. 1999..This review discusses various approaches to modifying cells genetically, both in vitro and in vivo, for the treatment of diabetes...
- beta-cell Regeneration: neogenesis, replication or both?Fred Levine
UCSD School of Medicine, Pediatrics, La Jolla, CA, USA
J Mol Med (Berl) 86:247-58. 2008..Clearly, understanding the nature and control of beta-cell regeneration is critical for success in efforts to treat diabetes by beta-cell replacement...
- β-Cell replication and islet neogenesis following partial pancreatectomySeung Hee Lee
Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA, USA
Islets 3:188-95. 2011..In summary, our data indicate that islet neogenesis following partial pancreatectomy does not occur...
- HES6 reverses nuclear reprogramming of insulin-producing cells following cell fusionAndrew J Ball
UCSD Department of Pediatrics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0816, USA
Biochem Biophys Res Commun 355:331-7. 2007..We conclude that the balance of Notch activators and inhibitors may play an important role in maintaining the beta-cell differentiated state...
- Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreasErgeng Hao
Rebecca and John Moores UCSD Cancer Center, University of California San Diego, 9500 Gilman Drive, MC 0816, La Jolla, California 92093, USA
Nat Med 12:310-6. 2006..Nonendocrine-to-endocrine differentiation of NEPECs supports the existence of endocrine stem or progenitor cells within the epithelial compartment of the adult human pancreas...
- Id3 upregulates BrdU incorporation associated with a DNA damage response, not replication, in human pancreatic β-cellsSeung Hee Lee
Sanford Children s Health Research Center, Sanford Burnham Institute for Medical Research, La Jolla, CA, USA
Islets 3:358-66. 2011..The data provide a much needed comparative model for investigating the molecular basis for this resistance in order to develop a strategy for improving replication competence in β-cells...
- Sources of beta-cells for human cell-based therapies for diabetesPamela Itkin-Ansari
Cancer Center, University of California San Diego School of Medicine, La Jolla, CA, USA
Cell Biochem Biophys 40:103-12. 2004..Further understanding of the factors governing beta-cell development are likely to be required before a robust cell-based therapy is available for the treatment of diabetes...
- The Id3/E47 axis mediates cell-cycle control in human pancreatic ducts and adenocarcinomaSeung Hee Lee
Department of Pediatrics, Universityof California San Diego, La Jolla, CA 92093, USA
Mol Cancer Res 9:782-90. 2011..Collectively, the data establish dysregulation of the Id/bHLH axis as an early and sustained feature of ductal pathogenesis and mark this axis as a potential therapeutic target for intervention in pancreatitis and PDA...
- Islet specific Wnt activation in human type II diabetesSeung Hee Lee
Development and Aging Program, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
Exp Diabetes Res 2008:728763. 2008..These data support a model in which the Wnt pathway plays a dynamic role in the pathogenesis of type II diabetes and suggest manipulation of Wnt signaling as a new approach to beta-cell-directed diabetes therapy...
- HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoterAlice Kiselyuk
Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
Chem Biol 19:806-18. 2012..The discovery of bioactive modulators for HNF4α raises the possibility that diseases involving HNF4α, such as diabetes and cancer, might be amenable to pharmacologic intervention by modulation of HNF4α activity...
- Cell-based therapies for diabetes: progress towards a transplantable human beta cell linePamela Itkin-Ansari
Cancer Center, University of California, San Diego, La Jolla, California 92093, USA
Ann N Y Acad Sci 1005:138-47. 2003..Here, we provide further information on the characteristics of cell lines that we have developed from the human pancreas that are relevant to the development of a beta cell replacement therapy for diabetes...
- Telomere-independent cellular senescence in human fetal cardiomyocytesAndrew J Ball
UCSD Cancer Center, 9500 Gilman Drive, La Jolla, CA 92093 0816, USA
Aging Cell 4:21-30. 2005..In addition to offering novel insights into the behavior of human fetal cardiomyocytes in culture, these findings have implications for the development of a cell-based therapy for cardiac injury using primary fetal heart tissue...
- CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cellsSeung Hee Lee
Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
Aging (Albany NY) 2:785-90. 2010..This finding has implications for efforts to induce beta-cell replication as a treatment for diabetes...
- Phenothiazine neuroleptics signal to the human insulin promoter as revealed by a novel high-throughput screenAlice Kiselyuk
Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA
J Biomol Screen 15:663-70. 2010....
- Coordinated regulation by Shp2 tyrosine phosphatase of signaling events controlling insulin biosynthesis in pancreatic beta-cellsSharon S Zhang
Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:7531-6. 2009..Therefore, Shp2 acts as a signal coordinator in beta-cells, orchestrating multiple pathways controlling insulin biosynthesis to maintain glucose homeostasis...
- Chronic treatment of mitochondrial disease patients with dichloroacetateBruce A Barshop
Department of Pediatrics, University of California San Diego, La Jolla, CA 92093 0830, USA
Mol Genet Metab 83:138-49. 2004....
- Gene therapy for diabetesC Demeterco
UCSD Cancer Center, La Jolla, CA. 92093-0912, USA
Front Biosci 6:D175-91. 2001..The focus of this review is on how gene therapy can be used in beta cell replacement strategies. Gene transfer to beta cells as well as recent advances in beta cell growth and development will be discussed...
- Current and future treatment strategies for type 2 diabetes: the beta-cell as a therapeutic targetB Tyrberg
UCSD Cancer Center, La Jolla, CA 92037 0912, USA
Curr Opin Investig Drugs 2:1568-74. 2001..The treatment strategies presented include promotion of beta-cell proliferation and differentiation by glucagon-like peptide 1 receptor agonists...
- NeuroD1 in the endocrine pancreas: localization and dual function as an activator and repressorP Itkin-Ansari
Cancer Center, University of California, San Diego, School of Medicine, Stem Cell Program, The Burnham Institute, La Jolla, California 92093 0816, USA
Dev Dyn 233:946-53. 2005....
- PAR2 regulates regeneration, transdifferentiation, and deathRon Piran
Sanford Children s Health Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA
Cell Death Dis 7:e2452. 2016..These studies provide a pharmacologically relevant target to induce tissue regeneration in a number of diseases, including T1D...
- Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cellsIgor I Katkov
UCSD Cancer Center, San Diego, CA 92122, USA Stem Cell Center Core, Burnham Institute for Medical Research BIMR, La Jolla, CA 92037, USA
Cryobiology 53:194-205. 2006..It is concluded that new protocols and media are required for freezing hESC and safe storage at -80 degrees C as well as studies of the mechanisms of stress-related events associated with cell cryopreservation...
- Diabetes mellitus-cell transplantation and gene therapy approachesT Halvorsen
UCSD Cancer Center, La Jolla, CA 92093-0912, USA
Curr Mol Med 1:273-86. 2001..This review focuses on approaches to developing robust and widely applicable beta-cell replacement strategies with an emphasis on manipulating beta-cell growth and differentiation by genetic engineering...
- c-Myc controls proliferation versus differentiation in human pancreatic endocrine cellsCarla Demeterco
Cancer Center, University of California San Diego, 1st Floor Building 7, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
J Clin Endocrinol Metab 87:3475-85. 2002..These results suggest that c-Myc plays a central role in a cell-cell contact-mediated switch mechanism by which cell division vs. differentiation in endocrine cells is determined...
- Efficient β-cell regeneration by a combination of neogenesis and replication following β-cell ablation and reversal of pancreatic duct ligationErgeng Hao
Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, California, USA Pediatric Diabetes Research Center, University of California, San Diego, California, USA
Stem Cells 31:2388-95. 2013..This provides an important proof of principle that efficient β-cell regeneration is possible, even from a starting point of profound β-cell ablation. This has important implications for efforts to promote β-cell regeneration...
- E2A gene products are not required for insulin gene expressionP Itkin-Ansari
Center for Molecular Genetics, Whittier Institute, Department of Pediatrics, University of California San Diego School of Medicine 92093 0634, USA
Endocrinology 137:3540-3. 1996..These data show that although E2A gene products probably play an important role in insulin gene expression, pancreatic development and insulin production can proceed in their absence...
- Induction of β-cell replication by a synthetic HNF4α antagonistSeung Hee Lee
Sanford Children s Health Research Center, Sanford Burnham Medical Research Institute, La Jolla, California, USA
Stem Cells 31:2396-407. 2013....
- Derivation of a retinoid X receptor scaffold from peroxisome proliferator-activated receptor gamma ligand 1-Di(1H-indol-3-yl)methyl-4-trifluoromethylbenzeneMarcia I Dawson
Cancer Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Rd, La Jolla, CA 92037, USA
ChemMedChem 4:1106-19. 2009....
- Characterization of ataxia telangiectasia fibroblasts with extended life-span through telomerase expressionL D Wood
Department of Biology, University of California San Diego, La Jolla, California, CA 92093-0322, USA
Oncogene 20:278-88. 2001..These immortalized A-T cells provide a more stable cell system to investigate the molecular mechanisms underlying the cellular phenotypes of Ataxia-telangiectasia...
- Presenilin 1 facilitates the constitutive turnover of beta-catenin: differential activity of Alzheimer's disease-linked PS1 mutants in the beta-catenin-signaling pathwayD E Kang
Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
J Neurosci 19:4229-37. 1999..These findings raise the intriguing possibility that PS1-beta-catenin interactions and subsequent activities may be consequential for the pathogenesis of AD...
- No pancreatic endocrine stem cells?Fred Levine
Burnham Institute and the University of California, San Diego, USA
N Engl J Med 351:1024-6. 2004
- High-Throughput Screen for Beta-Cell ReplicationFred Levine; Fiscal Year: 2006..By furthering our understanding of the mechanisms that control cell cycle entry in human beta-cells, we should be in an improved position to induce beta-cell regeneration in vivo and/or in vitro. ..