Louise C Laurent

Summary

Affiliation: University of California
Country: USA

Publications

  1. doi request reprint Comprehensive microRNA profiling reveals a unique human embryonic stem cell signature dominated by a single seed sequence
    Louise C Laurent
    The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Stem Cells 26:1506-16. 2008
  2. pmc MicroRNAs in embryonic stem cells and early embryonic development
    Louise C Laurent
    Department of Reproductive Medicine, Division of Maternal Fetal Medicine, University of California, San Diego, CA 92103 8433, USA
    J Cell Mol Med 12:2181-8. 2008
  3. pmc Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture
    Louise C Laurent
    Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Stem Cell 8:106-18. 2011
  4. pmc Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives
    Kristopher L Nazor
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Stem Cell 10:620-34. 2012
  5. pmc Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions
    Xiaoyan Liao
    Department of Reproductive Medicine, University of California, San Diego, California, USA
    J Cell Sci 126:3848-61. 2013
  6. pmc Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis
    Yu Chieh Wang
    The Scripps Research Institute, Department of Chemical Physiology, La Jolla, CA 92037, USA
    Cell Res 21:1551-63. 2011
  7. pmc Regulatory networks define phenotypic classes of human stem cell lines
    Franz Josef Muller
    Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 455:401-5. 2008
  8. doi request reprint Unraveling epigenetic regulation in embryonic stem cells
    Marina Bibikova
    Illumina, Inc, San Diego, CA 92121, USA
    Cell Stem Cell 2:123-34. 2008
  9. doi request reprint Spontaneous Single-Copy Loss of TP53 in Human Embryonic Stem Cells Markedly Increases Cell Proliferation and Survival
    Hadar Amir
    Department of Reproductive Medicine, UCSD Healthcare, 9500 Gilman Drive, Mail Code 0695, San Diego, CA, 92093, United States of America
    Stem Cells . 2016
  10. pmc Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions
    Ibon Garitaonandia
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States of America
    PLoS ONE 10:e0118307. 2015

Collaborators

Detail Information

Publications25

  1. doi request reprint Comprehensive microRNA profiling reveals a unique human embryonic stem cell signature dominated by a single seed sequence
    Louise C Laurent
    The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Stem Cells 26:1506-16. 2008
    ..We propose that miRNAs are coordinately controlled in hESCs, and are key regulators of pluripotence and differentiation. Disclosure of potential conflicts of interest is found at the end of this article...
  2. pmc MicroRNAs in embryonic stem cells and early embryonic development
    Louise C Laurent
    Department of Reproductive Medicine, Division of Maternal Fetal Medicine, University of California, San Diego, CA 92103 8433, USA
    J Cell Mol Med 12:2181-8. 2008
    ..Finally, we will point out evidence showing that there is strong and highly redundant miRNA regulation of a common set of target genes in the early stages of embryonic development and in ESCs...
  3. pmc Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture
    Louise C Laurent
    Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Cell Stem Cell 8:106-18. 2011
    ..Our results illustrate the dynamic nature of genomic abnormalities in pluripotent stem cells and the need for frequent genomic monitoring to assure phenotypic stability and clinical safety...
  4. pmc Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivatives
    Kristopher L Nazor
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Stem Cell 10:620-34. 2012
    ....
  5. pmc Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions
    Xiaoyan Liao
    Department of Reproductive Medicine, University of California, San Diego, California, USA
    J Cell Sci 126:3848-61. 2013
    ..These findings suggest that precise control of target mRNA expression by miRNAs ensures proper lineage specification during pancreatic development. ..
  6. pmc Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis
    Yu Chieh Wang
    The Scripps Research Institute, Department of Chemical Physiology, La Jolla, CA 92037, USA
    Cell Res 21:1551-63. 2011
    ....
  7. pmc Regulatory networks define phenotypic classes of human stem cell lines
    Franz Josef Muller
    Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 455:401-5. 2008
    ..Our results offer a new strategy for classifying stem cells and support the idea that pluripotency and self-renewal are under tight control by specific molecular networks...
  8. doi request reprint Unraveling epigenetic regulation in embryonic stem cells
    Marina Bibikova
    Illumina, Inc, San Diego, CA 92121, USA
    Cell Stem Cell 2:123-34. 2008
    ..Understanding the interactions among these factors will be critical to the development of improved strategies to reprogram differentiated cells or direct differentiation of pluripotent cells...
  9. doi request reprint Spontaneous Single-Copy Loss of TP53 in Human Embryonic Stem Cells Markedly Increases Cell Proliferation and Survival
    Hadar Amir
    Department of Reproductive Medicine, UCSD Healthcare, 9500 Gilman Drive, Mail Code 0695, San Diego, CA, 92093, United States of America
    Stem Cells . 2016
    ..1 deletion phenotype. Thus, sporadic mutations in hPSCs can have phenotypic effects that may impact their utility for clinical applications. This article is protected by copyright. All rights reserved...
  10. pmc Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions
    Ibon Garitaonandia
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States of America
    PLoS ONE 10:e0118307. 2015
    ..The effects of enzymatic passaging and feeder-free conditions were also observed in hiPSC cultures. Our results highlight the need for careful assessment of the effects of culture conditions on cells intended for clinical therapies. ..
  11. pmc Conversion of human fibroblasts to angioblast-like progenitor cells
    Leo Kurian
    Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Methods 10:77-83. 2013
    ..This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies...
  12. doi request reprint The functions of microRNAs in pluripotency and reprogramming
    Trevor R Leonardo
    Department of Chemical Physiology, The Scripps Research Institute and the Center for Regenerative Medicine, La Jolla, California 92037, USA
    Nat Cell Biol 14:1114-21. 2012
    ..Detailed studies of miRNAs and their targets in PSCs should help to parse the regulatory networks that underlie developmental processes and cellular reprogramming...
  13. pmc DNA methylation in embryonic stem cells
    Gulsah Altun
    Department of Reproductive Medicine, University of California, San Diego, California 92103, USA
    J Cell Biochem 109:1-6. 2010
    ..In this article, we highlight these methods and discuss recent DNA methylation studies on ESCs...
  14. pmc Stage-specific regulation of the WNT/β-catenin pathway enhances differentiation of hESCs into hepatocytes
    Thomas Touboul
    Department of Reproductive Medicine, UC San Diego, La Jolla, CA 92037, United States
    J Hepatol 64:1315-26. 2016
    ..We hypothesize that the functionality of hPSC-derived hepatocytes might be improved by making the differentiation method more similar to normal in vivo hepatic development...
  15. pmc Establishment of human iPSC-based models for the study and targeting of glioma initiating cells
    Ignacio Sancho-Martinez
    Gene Expression Laboratory Belmonte, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Commun 7:10743. 2016
    ..Last, screening of 101 anti-cancer compounds identifies three molecules specifically targeting transformed iNPCs and primary GTICs. Together, our results highlight the potential of hiPSCs for studying human tumourigenesis. ..
  16. doi request reprint Abnormal uterine artery Doppler velocimetry predicts adverse outcomes in patients with abnormal analytes
    Hilary A Roeder
    UC San Diego Health System, Department of Reproductive Medicine, 200 W Arbor Drive MC 8433, San Diego, CA 92103, USA Electronic address
    Pregnancy Hypertens 4:296-301. 2014
    ....
  17. pmc BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell state
    Yingchun Li
    Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
    Development 140:3965-76. 2013
    ....
  18. pmc Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells
    Mason A Israel
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Nature 482:216-20. 2012
    ..More generally, we demonstrate that iPSC technology can be used to observe phenotypes relevant to Alzheimer's disease, even though it can take decades for overt disease to manifest in patients...
  19. pmc Friedreich's ataxia induced pluripotent stem cells model intergenerational GAA⋅TTC triplet repeat instability
    Sherman Ku
    Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Stem Cell 7:631-7. 2010
    ....
  20. pmc Miniaturization Technologies for Efficient Single-Cell Library Preparation for Next-Generation Sequencing
    Sergio Mora-Castilla
    Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA
    J Lab Autom 21:557-67. 2016
    ..Thus, we have developed a process to enable efficient and cost-effective high-throughput single-cell transcriptome sequencing. ..
  21. doi request reprint Maternal obesity and sex-specific differences in placental pathology
    Sandra M Leon-Garcia
    Department of Reproductive Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Placenta 38:33-40. 2016
    ..We sought to investigate the influence of pregravid obesity and fetal sex on placental histopathology while controlling for diabetes and hypertension...
  22. doi request reprint The epigenome in pluripotency and differentiation
    Rathi D Thiagarajan
    1 Department of Reproductive Medicine, The University of California, San Diego, La Jolla, CA, USA
    Epigenomics 6:121-37. 2014
    ..This review focuses on the two most well-studied epigenetic mechanisms, DNA methylation and histone modifications, within the context of pluripotency and differentiation. ..
  23. pmc Getting off the ground state: X chromosome inactivation knocks down barriers to differentiation
    Robert Morey
    Department of Reproductive Medicine, University of California, San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA 92037, USA
    Cell Stem Cell 14:131-2. 2014
    ..Schulz et al. (2014) now demonstrate that the presence of two active X chromosomes in mESCs prevents exit from pluripotency by blocking MAPK signaling, ensuring synchronization between X chromosome dosage compensation and development...
  24. pmc Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy
    Suzanne E Peterson
    Department of Molecular Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 6:e23018. 2011
    ..The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation...
  25. doi request reprint Induced pluripotent stem cells from highly endangered species
    Inbar Friedrich Ben-Nun
    Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA
    Nat Methods 8:829-31. 2011
    ..iPSCs may eventually facilitate reintroduction of genetic material into breeding populations...