Louise C Laurent
Affiliation: University of California
- Comprehensive microRNA profiling reveals a unique human embryonic stem cell signature dominated by a single seed sequenceLouise C Laurent
The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Stem Cells 26:1506-16. 2008..We propose that miRNAs are coordinately controlled in hESCs, and are key regulators of pluripotence and differentiation. Disclosure of potential conflicts of interest is found at the end of this article...
- MicroRNAs in embryonic stem cells and early embryonic developmentLouise C Laurent
Department of Reproductive Medicine, Division of Maternal Fetal Medicine, University of California, San Diego, CA 92103 8433, USA
J Cell Mol Med 12:2181-8. 2008..Finally, we will point out evidence showing that there is strong and highly redundant miRNA regulation of a common set of target genes in the early stages of embryonic development and in ESCs...
- Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in cultureLouise C Laurent
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Cell Stem Cell 8:106-18. 2011..Our results illustrate the dynamic nature of genomic abnormalities in pluripotent stem cells and the need for frequent genomic monitoring to assure phenotypic stability and clinical safety...
- Recurrent variations in DNA methylation in human pluripotent stem cells and their differentiated derivativesKristopher L Nazor
Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Cell Stem Cell 10:620-34. 2012....
- Matched miRNA and mRNA signatures from an hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactionsXiaoyan Liao
Department of Reproductive Medicine, University of California, San Diego, California, USA
J Cell Sci 126:3848-61. 2013..These findings suggest that precise control of target mRNA expression by miRNAs ensures proper lineage specification during pancreatic development. ..
- Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysisYu Chieh Wang
The Scripps Research Institute, Department of Chemical Physiology, La Jolla, CA 92037, USA
Cell Res 21:1551-63. 2011....
- Regulatory networks define phenotypic classes of human stem cell linesFranz Josef Muller
Center for Regenerative Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 455:401-5. 2008..Our results offer a new strategy for classifying stem cells and support the idea that pluripotency and self-renewal are under tight control by specific molecular networks...
- Unraveling epigenetic regulation in embryonic stem cellsMarina Bibikova
Illumina, Inc, San Diego, CA 92121, USA
Cell Stem Cell 2:123-34. 2008..Understanding the interactions among these factors will be critical to the development of improved strategies to reprogram differentiated cells or direct differentiation of pluripotent cells...
- Conversion of human fibroblasts to angioblast-like progenitor cellsLeo Kurian
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA
Nat Methods 10:77-83. 2013..This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies...
- The functions of microRNAs in pluripotency and reprogrammingTrevor R Leonardo
Department of Chemical Physiology, The Scripps Research Institute and the Center for Regenerative Medicine, La Jolla, California 92037, USA
Nat Cell Biol 14:1114-21. 2012..Detailed studies of miRNAs and their targets in PSCs should help to parse the regulatory networks that underlie developmental processes and cellular reprogramming...
- DNA methylation in embryonic stem cellsGulsah Altun
Department of Reproductive Medicine, University of California, San Diego, California 92103, USA
J Cell Biochem 109:1-6. 2010..In this article, we highlight these methods and discuss recent DNA methylation studies on ESCs...
- BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell stateYingchun Li
Department of Pathology, University of California San Diego, La Jolla, CA 92093, USA
Development 140:3965-76. 2013....
- Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cellsMason A Israel
Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
Nature 482:216-20. 2012..More generally, we demonstrate that iPSC technology can be used to observe phenotypes relevant to Alzheimer's disease, even though it can take decades for overt disease to manifest in patients...
- Friedreich's ataxia induced pluripotent stem cells model intergenerational GAA⋅TTC triplet repeat instabilitySherman Ku
Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Cell Stem Cell 7:631-7. 2010....
- Getting off the ground state: X chromosome inactivation knocks down barriers to differentiationRobert Morey
Department of Reproductive Medicine, University of California, San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA 92037, USA
Cell Stem Cell 14:131-2. 2014..Schulz et al. (2014) now demonstrate that the presence of two active X chromosomes in mESCs prevents exit from pluripotency by blocking MAPK signaling, ensuring synchronization between X chromosome dosage compensation and development...
- The epigenome in pluripotency and differentiationRathi D Thiagarajan
1 Department of Reproductive Medicine, The University of California, San Diego, La Jolla, CA, USA
Epigenomics 6:121-37. 2014..This review focuses on the two most well-studied epigenetic mechanisms, DNA methylation and histone modifications, within the context of pluripotency and differentiation. ..
- Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidySuzanne E Peterson
Department of Molecular Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America
PLoS ONE 6:e23018. 2011..The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation...
- Induced pluripotent stem cells from highly endangered speciesInbar Friedrich Ben-Nun
Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA
Nat Methods 8:829-31. 2011..iPSCs may eventually facilitate reintroduction of genetic material into breeding populations...