VERA KRYMSKAYA

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase
    Elena Goncharova
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 167:1171-82. 2004
  2. pmc Differential effects of formoterol on thrombin- and PDGF-induced proliferation of human pulmonary arterial vascular smooth muscle cells
    Elena A Goncharova
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA
    Respir Res 13:109. 2012
  3. pmc PI3K/mTORC1 activation in hamartoma syndromes: therapeutic prospects
    Vera P Krymskaya
    Department of Medicine, and Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104 3403, USA
    Cell Cycle 8:403-13. 2009
  4. pmc Smooth muscle-like cells in pulmonary lymphangioleiomyomatosis
    Vera P Krymskaya
    Pulmonary Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 3403, USA
    Proc Am Thorac Soc 5:119-26. 2008
  5. ncbi request reprint Targeting the phosphatidylinositol 3-kinase pathway in airway smooth muscle: rationale and promise
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 3403, USA
    BioDrugs 21:85-95. 2007
  6. ncbi request reprint Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells
    V P Krymskaya
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Lung Cell Mol Physiol 280:L1009-18. 2001
  7. ncbi request reprint Src is necessary and sufficient for human airway smooth muscle cell proliferation and migration
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Blvd, BRB II III, Philadelphia, PA 19104 6160, USA
    FASEB J 19:428-30. 2005
  8. ncbi request reprint Tumour suppressors hamartin and tuberin: intracellular signalling
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, 847 BRB II III, Philadelphia, PA 19104 6160, USA
    Cell Signal 15:729-39. 2003
  9. ncbi request reprint Lymphangioleiomyomatosis: a complex tale of serum response factor-mediated tissue inhibitor of metalloproteinase-3 regulation
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Am J Respir Cell Mol Biol 28:546-50. 2003
  10. ncbi request reprint Tuberin regulates p70 S6 kinase activation and ribosomal protein S6 phosphorylation. A role for the TSC2 tumor suppressor gene in pulmonary lymphangioleiomyomatosis (LAM)
    Elena A Goncharova
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, 847 BRB II III, Philadelphia, PA 19104, USA
    J Biol Chem 277:30958-67. 2002

Research Grants

  1. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2002
  2. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    Vera P Krymskaya; Fiscal Year: 2010
  3. The Role of TSC2 and Rho GTPases in LAM
    VERA KRYMSKAYA; Fiscal Year: 2009
  4. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2009
  5. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2007
  6. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2005
  7. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2004
  8. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2003
  9. The Role of TSC2 and Rho GTPases in LAM
    Vera P Krymskaya; Fiscal Year: 2010

Collaborators

Detail Information

Publications28

  1. pmc TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase
    Elena Goncharova
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 167:1171-82. 2004
    ....
  2. pmc Differential effects of formoterol on thrombin- and PDGF-induced proliferation of human pulmonary arterial vascular smooth muscle cells
    Elena A Goncharova
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA
    Respir Res 13:109. 2012
    ..The goals of this study were to examine effects of racemic formoterol and its enantiomers on PAVSM cell proliferation as it relates to COPD-associated PH...
  3. pmc PI3K/mTORC1 activation in hamartoma syndromes: therapeutic prospects
    Vera P Krymskaya
    Department of Medicine, and Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104 3403, USA
    Cell Cycle 8:403-13. 2009
    ....
  4. pmc Smooth muscle-like cells in pulmonary lymphangioleiomyomatosis
    Vera P Krymskaya
    Pulmonary Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 3403, USA
    Proc Am Thorac Soc 5:119-26. 2008
    ..In this article, we will summarize the progress made in our understanding of LAM, and we will focus on how dysregulation of TSC1/TSC2 signaling results in abnormal proliferation and migration of SM-like LAM cells...
  5. ncbi request reprint Targeting the phosphatidylinositol 3-kinase pathway in airway smooth muscle: rationale and promise
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 3403, USA
    BioDrugs 21:85-95. 2007
    ..This article highlights the potential contribution of the PI3K-TSC1/TSC2-mTOR/S6K1 pathway in smooth muscle remodeling. Pharmacologic manipulation of this signaling pathway could have a major impact on treatment of asthma and COPD...
  6. ncbi request reprint Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells
    V P Krymskaya
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Lung Cell Mol Physiol 280:L1009-18. 2001
    ..Collectively, these data suggest that class IA PI3K is activated by thrombin and is sufficient to induce ASM cell growth...
  7. ncbi request reprint Src is necessary and sufficient for human airway smooth muscle cell proliferation and migration
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Blvd, BRB II III, Philadelphia, PA 19104 6160, USA
    FASEB J 19:428-30. 2005
    ....
  8. ncbi request reprint Tumour suppressors hamartin and tuberin: intracellular signalling
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, 847 BRB II III, Philadelphia, PA 19104 6160, USA
    Cell Signal 15:729-39. 2003
    ....
  9. ncbi request reprint Lymphangioleiomyomatosis: a complex tale of serum response factor-mediated tissue inhibitor of metalloproteinase-3 regulation
    Vera P Krymskaya
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Am J Respir Cell Mol Biol 28:546-50. 2003
  10. ncbi request reprint Tuberin regulates p70 S6 kinase activation and ribosomal protein S6 phosphorylation. A role for the TSC2 tumor suppressor gene in pulmonary lymphangioleiomyomatosis (LAM)
    Elena A Goncharova
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, 847 BRB II III, Philadelphia, PA 19104, USA
    J Biol Chem 277:30958-67. 2002
    ..These data demonstrate that tuberin negatively regulates the activity of S6 and p70S6K specifically, and suggest a potential mechanism for abnormal cell growth in LAM...
  11. ncbi request reprint Phosphatidylinositol 3-kinase but not tuberin is required for PDGF-induced cell migration
    Carla Irani
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Am J Physiol Lung Cell Mol Physiol 282:L854-62. 2002
    ..These data suggest that PI 3-kinase is required for growth factor-induced cell migration and loss of tuberin appears to have little effect on cell migration...
  12. ncbi request reprint PI3K is required for proliferation and migration of human pulmonary vascular smooth muscle cells
    Elena A Goncharova
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 6160, USA
    Am J Physiol Lung Cell Mol Physiol 283:L354-63. 2002
    ..These results demonstrate that PI3K signaling is both necessary and sufficient to mediate human PVSM cell proliferation and migration and suggest that the activation of PI3K may play an important role in vascular remodeling...
  13. ncbi request reprint Vascular endothelial growth factor-induced secretion of fibronectin is ERK dependent
    Altaf S Kazi
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Am J Physiol Lung Cell Mol Physiol 286:L539-45. 2004
    ..We conclude that ASM-derived VEGF, through modulation of the extracellular matrix, may play an important role in airway remodeling seen in asthma...
  14. pmc Interferons modulate mitogen-induced protein synthesis in airway smooth muscle
    Elena A Goncharova
    Pulmonary, Allergy, and Critical Care Division, Airway Biology Initiative, Department of Medicine, Cardiovascular Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Am J Physiol Lung Cell Mol Physiol 299:L25-35. 2010
    ....
  15. ncbi request reprint Pulmonary lymphangioleiomyomatosis (LAM): progress and current challenges
    Elena A Goncharova
    Department of Medicine, University of Pennsylvania, Pennsylvania, USA
    J Cell Biochem 103:369-82. 2008
    ..In this review, we will summarize the progress made in our understanding of TSC1/TSC2 cellular signaling and the molecular mechanisms of LAM; we will also highlight some of the lesser explored directions and challenges in LAM research...
  16. pmc Signal transducer and activator of transcription 3 is required for abnormal proliferation and survival of TSC2-deficient cells: relevance to pulmonary lymphangioleiomyomatosis
    Elena A Goncharova
    Pulmonary, Allergy, and Critical Care Division, Airways Biology Initiative, Department of Medicine, University of Pennsylvania, TRL, Room 1214, 125 South 31st St, Philadelphia, PA 19104, USA
    Mol Pharmacol 76:766-77. 2009
    ..Thus, STAT3 may provide a novel therapeutic target for diseases associated with TSC1/TSC2 dysfunction...
  17. ncbi request reprint Interferon beta augments tuberous sclerosis complex 2 (TSC2)-dependent inhibition of TSC2-null ELT3 and human lymphangioleiomyomatosis-derived cell proliferation
    Elena A Goncharova
    Department of Medicine, University of Pennsylvania, TRL Suite 1200, 125 South 31st Street, Philadelphia, PA 19104, USA
    Mol Pharmacol 73:778-88. 2008
    ....
  18. pmc Abnormal growth of smooth muscle-like cells in lymphangioleiomyomatosis: Role for tumor suppressor TSC2
    Elena A Goncharova
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, 421 Curie Boulevard, BRB II III, Philadelphia, PA 19104 6160, USA
    Am J Respir Cell Mol Biol 34:561-72. 2006
    ..Loss of TSC2 GAP activity or disruption of the TSC1/TSC2 complex dysregulates S6K1 activation, which leads to abnormal cell proliferation associated with LAM disease...
  19. ncbi request reprint IFN-gamma inhibits human airway smooth muscle cell proliferation by modulating the E2F-1/Rb pathway
    Yassine Amrani
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Lung Cell Mol Physiol 284:L1063-71. 2003
    ....
  20. pmc Modulation of cell migration and invasiveness by tumor suppressor TSC2 in lymphangioleiomyomatosis
    Elena A Goncharova
    Department of Medicine, University of Pennsylvania, BRB II III, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Am J Respir Cell Mol Biol 34:473-80. 2006
    ....
  21. ncbi request reprint Cyclic AMP-mobilizing agents and glucocorticoids modulate human smooth muscle cell migration
    Elena A Goncharova
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Am J Respir Cell Mol Biol 29:19-27. 2003
    ..Collectively, these findings demonstrate that cAMP-mobilizing agents and steroids modulate human smooth muscle cell migration, likely by distinct mechanisms...
  22. ncbi request reprint The PP2A-associated protein alpha4 plays a critical role in the regulation of cell spreading and migration
    Mei Kong
    Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:29712-20. 2007
    ..Together, these data define a novel role for the protein phosphatase 2A regulatory subunit alpha4 in the regulation of cell spreading and migration...
  23. ncbi request reprint Airway smooth muscle cells enhance C3a-induced mast cell degranulation following cell-cell contact
    E Berla Thangam
    Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104 6002, USA
    FASEB J 19:798-800. 2005
    ....
  24. ncbi request reprint Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration
    Elena A Goncharova
    Department of Medicine, Pulmonary, Allergy and Critical Care Division, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Protoc 1:2933-9. 2006
    ....
  25. ncbi request reprint Assays for in vitro monitoring of proliferation of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cells
    Elena A Goncharova
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Protoc 1:2905-8. 2006
    ..Although using radiolabeled [3H]thymidine incorporation is a limitation, the greatest benefit of the assay is providing reliable and statistically significant data...
  26. pmc mTORC2 is required for proliferation and survival of TSC2-null cells
    Elena A Goncharova
    Pulmonary, Allergy and Critical Care Division, Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Cell Biol 31:2484-98. 2011
    ....
  27. ncbi request reprint Phosphodiesterases regulate airway smooth muscle function in health and disease
    Vera P Krymskaya
    Department of Medicine, Pulmonary, Allergy, and Critical Care Division, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Curr Top Dev Biol 79:61-74. 2007
    ..This chapter will focus on recent advances in our understanding of the role of PDE in regulating ASM function...
  28. ncbi request reprint Cross-talk between tuberin, calmodulin, and estrogen signaling pathways
    Brian York
    Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky 40536 0298, USA
    FASEB J 19:1202-4. 2005
    ..Cumulatively, data presented here support the hypothesis that interactions between tuberin, ERalpha, and CaM may play a critical role in the pathology of LAM disease...

Research Grants11

  1. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2002
    ....
  2. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    Vera P Krymskaya; Fiscal Year: 2010
    ....
  3. The Role of TSC2 and Rho GTPases in LAM
    VERA KRYMSKAYA; Fiscal Year: 2009
    ..This study will elucidate the role of tumor suppressor tuberous sclerosis complex 2 (TSC2) and Rho family of small GTPases in LAM and will identify novel targets for combinational pharmaceutical intervention to treat this disease. ..
  4. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2009
    ....
  5. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2007
    ....
  6. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2005
    ....
  7. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2004
    ....
  8. Role of p70 S6 Kinase in Regulating LAM Cell Growth
    VERA KRYMSKAYA; Fiscal Year: 2003
    ....
  9. The Role of TSC2 and Rho GTPases in LAM
    Vera P Krymskaya; Fiscal Year: 2010
    ..This study will elucidate the role of tumor suppressor tuberous sclerosis complex 2 (TSC2) and Rho family of small GTPases in LAM and will identify novel targets for combinational pharmaceutical intervention to treat this disease. ..