Genomes and Genes
Affiliation: University of Alabama at Birmingham
- Attenuation of neurodegeneration-relevant modifications of brain proteins by dietary soyH Kim
Department of Pharmacology and Toxicology and the UAB Center for Aging, University of Alabama at Birmingham, 35294 0019, USA
Biofactors 12:243-50. 2000..The rationale is discussed for the use of soy-based foods for protection against postmenopausal neurodegeneration...
- Chemoprevention by grape seed extract and genistein in carcinogen-induced mammary cancer in rats is diet dependentHelen Kim
Departments of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J Nutr 134:3445S-3452S. 2004....
- Actions of the soy phytoestrogen genistein in models of human chronic disease: potential involvement of transforming growth factor betaH Kim
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1670 University Boulevard, Birmingham, AL 35294 0019, U S A
Biochem Soc Trans 29:216-22. 2001..The results discussed here indicate that genistein, and probably other related phytoestrogens, have pleiotropic actions, some of which may involve TGFbeta activity...
- Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compoundsHelen Kim
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, AL 35294, USA
Life Sci 78:2060-5. 2006..The fact that GSE affects proteins implicated in cognitive disorders suggests moreover that GSE may have impact on the actions of psychoactive drugs by maintaining an overall viability of the nervous system...
- 2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomesHelen Kim
Departments of Pharmacology and Toxicology, the UAB Center for Nutrient Gene Interaction, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
J Proteome Res 7:4638-50. 2008..This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points...
- Proteomics and mass spectrometry in nutrition researchHelen Kim
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Nutrition 20:155-65. 2004
- 2D gel proteomics: an approach to study age-related differences in protein abundance or isoform complexity in biological samplesHelen Kim
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
Methods Mol Biol 371:349-91. 2007....
- Proteomics analysis of rat brain protein modulations by grape seed extractJessy Deshane
Department of Pharmacology and Toxicology, University of Alabama at Birmingham Comprehensive Cancer Center, Birmingham, Alabama 35294, USA
J Agric Food Chem 52:7872-83. 2004..This is the first identification and quantitation of specific proteins in mammalian tissues modulated by a dietary supplement, as well as the first to demonstrate links of such proteins with any disease...
- Simvastatin enhances learning and memory independent of amyloid load in miceLing Li
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
Ann Neurol 60:729-39. 2006..Our objective was to determine whether administering a statin drug (simvastatin) would protect against the development of behavioral deficits in an established mouse model of Alzheimer's disease...
- Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extractJeevan K Prasain
Department of Pharmacology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Phytomedicine 16:233-43. 2009..Using LC-MS/MS, (+)-catechin and (-)-epicatechin were identified in the brain conclusively. These data suggested that GSE catechins cross the blood brain barrier and may be responsible for the neuroprotective effects of GSE...
- Lack of LDL receptor aggravates learning deficits and amyloid deposits in Alzheimer transgenic miceDongfeng Cao
Atherosclerosis Research Unit, Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, BDB 658, Birmingham, AL 35294, USA
Neurobiol Aging 27:1632-43. 2006..Our results suggest that the LDLR plays a role in the development of Alzheimer-type learning impairment and amyloidosis and can be a novel therapeutic target for AD...
- The case for well-conducted experiments to validate statistical protocols for 2D gels: different pre-processing = different lists of significant proteinsSreelatha Meleth
Biostatistics and Bioinformatics Unit, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
BMC Biotechnol 5:7. 2005..However, there is a dearth of systematic studies that have investigated appropriate statistical approaches to analyse the data from these experiments...
- New nutrition, proteomics, and how both can enhance studies in cancer prevention and therapyHelen Kim
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1918 University Blvd, Birmingham, AL 35294, USA
J Nutr 135:2715-8. 2005..This report illustrates how experimental approaches that combine proteomics technologies with a dietary intervention with specific phytochemicals in normal animals can enhance studies on cancer prevention and treatment...
- High-resolution mass spectrometry analysis of protein oxidations and resultant loss of functionStephen Barnes
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Biochem Soc Trans 36:1037-44. 2008..These results suggest that future in vivo studies should carefully assess the critical sites that are modified rather than using antibodies that do not distinguish between different modified sites...
- 2D-difference gel electrophoretic proteomic analysis of a cell culture model of alveolar rhabdomyosarcomaJoseph G Pressey
Department of Pediatrics, Division of Hematology Oncology, University of Alabama at Birmingham, Birmingham, AL 35233, USA
J Proteome Res 10:624-36. 2011..Thus, the 2D-DIGE proteomics methodology described here provided an important discovery approach to the study of RMS biology and complements the findings of previous mRNA expression studies...
- Modification of the mitochondrial proteome in response to the stress of ethanol-dependent hepatotoxicityAparna Venkatraman
Department of Pathology, University of Alabama, Birmingham, Alabama 35294, USA
J Biol Chem 279:22092-101. 2004..These data also suggest that the response of mitochondria to stress may not involve non-discriminate changes in the proteome but is restricted to those metabolic pathways that have a direct role in a specific pathology...
- Immunization of Alzheimer model mice with adenovirus vectors encoding amyloid beta-protein and GM-CSF reduces amyloid load in the brainHong Duck Kim
Department of Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham, KHGB 640B, 720 20th Street South, Birmingham, AL 35294 0024, USA
Neurosci Lett 370:218-23. 2004..Thus, intranasal administration of adenovirus vectors encoding Abeta and GM-CSF may be effective in prevention and treatment of AD...
- Nutriproteomics: identifying the molecular targets of nutritive and non-nutritive components of the dietStephen Barnes
Departments of Pharmacology and Toxicology, 452 McCallum Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
J Biochem Mol Biol 37:59-74. 2004..These are described, as are the pros and cons of each method in current use...
- Antihypertensive and cognitive effects of grape polyphenols in estrogen-depleted, female, spontaneously hypertensive ratsNing Peng
Dept of Cell Biology, University of Alabama at Birmingham, 1900 Univ Blvd THT 950, Birmingham, AL 35294 0006, USA
Am J Physiol Regul Integr Comp Physiol 289:R771-5. 2005..These results indicate that grape seed polyphenols decrease arterial pressure in SHR, probably via an antioxidant mechanism...
- active site modifications of the brain isoform of creatine kinase by 4-hydroxy-2-nonenal correlate with reduced enzyme activity: mapping of modified sites by Fourier transform-ion cyclotron resonance mass spectrometryShannon M Eliuk
Department of Pharmacology Toxicology, UAB Biomedical FT ICR MS Laboratory, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, LA 35294, USA
Chem Res Toxicol 20:1260-8. 2007....
- Mechanisms of action of the soy isoflavone genistein: emerging role for its effects via transforming growth factor beta signaling pathwaysH Kim
Department of Pharmacology and Toxicology, Comprehensive Cancer Center, University of Alabama at Birmingham, 35294 0019, USA
Am J Clin Nutr 68:1418S-1425S. 1998..These preclinical studies in combination with our cell culture data suggest that the mechanism of genistein involves, if not requires, TGFbeta1-signaling...
- In nutrition, can we "see" what is good for us?Stephen Barnes
Department of Pharmacology and Toxicology, Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
Adv Nutr 4:327S-34S. 2013..Extensive metabolism by phase I/II enzymes and by the gut microbiome may also create compounds that the eye is never allowed to appreciate...
- 2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cellsJ Tyson DeAngelis
Department of Biology, UAB Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, Alabama 35294 1170, USA
J Proteome Res 10:447-58. 2011....
- Cautions and research needs identified at the equol, soy, and menopause research leadership conferenceStephen Barnes
Department of Pharmacology and Toxicology, and Center for Nutrient Gene Interaction, University of Alabama at Birmingham and Purdue University University of Alabama at Birmingham Botanicals Research Center for Age Related Disease, Birmingham, AL 35294, USA
J Nutr 140:1390S-4S. 2010....
- Curcumin reverses breast tumor exosomes mediated immune suppression of NK cell tumor cytotoxicityHuang Ge Zhang
Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Biochim Biophys Acta 1773:1116-23. 2007..These data suggest that partial reversal of tumor exosome-mediated inhibition of NK cell tumor cytotoxicity may account for the anti-cancer properties of curcumin...
- The UAB Proteomics DatabaseAubrey Hill
UAB Comprehensive Cancer Center, UAB Mass Spectrometry and Proteomics Shared Facility, University of Alabama at Birmingham, 1530 3rd Ave South, Kaul 602A, Birmingham, AL 35294, USA
Bioinformatics 19:2149-51. 2003..Images of the gels from which each of these proteins was isolated are accessed by hyperlinks embedded in the database...
- High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of mitochondria and signaling complexesPaul S Brookes
Department of Pathology, University of Alabama at Birmingham, Biomedical Research Building 2, 901 19th Street South, Birmingham, AL 35294, USA
Proteomics 2:969-77. 2002..Applications of this functional mitochondrial proteomics method are discussed...
- Proteomic analysis of mouse hypothalamus under simulated microgravityPoonam Sarkar
Department of Pharmacology and Toxicology, University of Alabama, Birmingham, AL, 35294, USA
Neurochem Res 33:2335-41. 2008..Taken together the results reported here indicate that oxidative imbalance occurred in the hypothalamus in response to simulated microgravity...
- DING, a genistein target in human breast cancer: a protein without a geneMichael Belenky
Department of Biochemistry and Molecular Genetics and Pharmacology and Toxicology, University of Alabama at Birmingham, AL 35294, USA
J Nutr 133:2497S-2501S. 2003..The DING protein (N-terminal amino acid sequence Asp-Ile-Asn-Gly) that binds to genistein with high affinity is one of these. Its biological role, however, remains to be defined...
- Association of aortic atherosclerosis with cerebral beta-amyloidosis and learning deficits in a mouse model of Alzheimer's diseaseLing Li
Departments of Medicine, Pharmacology, and Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Am J Pathol 163:2155-64. 2003..Our results suggest that synergistic mechanisms may be involved in the pathogenesis of atherosclerosis and AD. These findings may have important implications in the prevention and treatment of cardiovascular diseases as well as AD...
- Prospective highlights of functional skin proteomicsChun Ming Huang
Department of Dermatology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Mass Spectrom Rev 24:647-60. 2005..This type of research into functional skin proteomics will provide a critical step toward understanding disease and developing successful therapeutic strategies...
- VCP/p97 AAA-ATPase does not interact with the endogenous wild-type cystic fibrosis transmembrane conductance regulatorRebecca F Goldstein
Department of Cell Biology, Comprehensive Cancer Center, and Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
Am J Respir Cell Mol Biol 36:706-14. 2007....
- Production of a novel class of polyreactive pathogenic autoantibodies in BXD2 mice causes glomerulonephritis and arthritisHui Chen Hsu
University of Alabama at Birmingham, 701 South 19th Street, Birmingham, AL 35294, USA
Arthritis Rheum 54:343-55. 2006..The goal of this study was to identify the antigenic target proteins and epitopes and to unravel the mechanisms by which the related conditions arise in BXD2 mice...
- Dr Helen Kim's story: the risk to blossom and glad to be exactly meKim Helen
University of Alabama at Birmingham, USA
J Cancer Educ 22:202-3. 2007
- Soy isoflavonoids and cancer -- metabolism at the target siteB J Boersma
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, VH G009, 1670 University Boulevard, Birmingham, AL 35294, USA
Mutat Res 480:121-7. 2001..Future investigations of GEN and other polyphenols must, therefore, take into account metabolism at the tissue site...
- Insulin resistance, dietary cholesterol, and cholesterol concentration in postmenopausal womenG M Reaven
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
Metabolism 50:594-7. 2001....
- Anti-Abeta single-chain antibody delivery via adeno-associated virus for treatment of Alzheimer's diseaseKen Ichiro Fukuchi
Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, PO Box 1649, Peoria, IL 61656, USA
Neurobiol Dis 23:502-11. 2006..Because the scFv lacks the Fc portion of the immunoglobulin molecule, this modality may be a feasible solution for AD without eliciting inflammation...
- Botanicals for age-related diseases: from field to practiceConnie M Weaver
Foods and Nutrition, Rutgers University, New Brunswick, NJ, USA
Am J Clin Nutr 87:493S-7S. 2008..Botanicals and their relation to bone antiresorptive capacity, cognitive function, vascular effects, and cancer are principal themes in our center...
- Amelioration of amyloid load by anti-Abeta single-chain antibody in Alzheimer mouse modelKen Ichiro Fukuchi
Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Box 1649, Peoria, IL 61656, USA
Biochem Biophys Res Commun 344:79-86. 2006..Because scFvs lack the Fc portion of the immunoglobulin molecule, human scFvs against Abeta may be useful to treat AD patients without eliciting brain inflammation...
- Proteomic analysis of mice hippocampus in simulated microgravity environmentPoonam Sarkar
Molecular Neurotoxicology Laboratory and Proteomics Core, Department of Biology, Texas Southern University, Houston, Texas 77004, USA
J Proteome Res 5:548-53. 2006..This preliminary investigation leads to an understanding of the alteration of proteins in the hippocampus in response to the microgravity environment...
- Increasing incidence of late second malignancies after conditioning with cyclophosphamide and total-body irradiation and autologous bone marrow transplantation for non-Hodgkin's lymphomaJennifer R Brown
Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
J Clin Oncol 23:2208-14. 2005..We report the incidence and outcome of solid tumors at 10-year follow-up in a large cohort of uniformly treated patients who underwent ABMT for non-Hodgkin's lymphoma (NHL)...
- The Prevalence of CYP2C8, 2C9, 2J2, and soluble epoxide hydrolase polymorphisms in African Americans with hypertensionAlbert W Dreisbach
Divisions of Nephrology and Clinical Pharmacology, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA
Am J Hypertens 18:1276-81. 2005..We performed a case-control study to determine the prevalence of epoxygenase-related SNP in African American individuals and to evaluate whether these SNP are associated with increased risk of hypertension...
- Comparison of sargramostim and filgrastim in the treatment of chemotherapy-induced neutropeniaDawn Marie Stull
Department of Pharmacy, New York Presbyterian Hospital New York Weill Cornell Center, New York, NY 10128, USA
Am J Health Syst Pharm 62:83-7. 2005
- Generation and characterization of monoclonal antibodies to human keratinocyte growth factor receptorPing Wei
Department of Hematology, Amgen Inc, Thousand Oaks, California 91320 1789, USA
Hybridoma (Larchmt) 25:115-24. 2006..Antagonistic MAbs were identified using a cell-based functional assay. These KGFR MAbs will be important reagents for studying the biological function and tissue distribution of this receptor in normal and pathological conditions...
- Exploiting the apoptotic actions of oestrogen to reverse antihormonal drug resistance in oestrogen receptor positive breast cancer patientsV Craig Jordan
Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
Breast 16:S105-13. 2007..Once our program is complete, the new knowledge will be available to translate to clinical care for the long-term maintenance of patients on antihormone therapy...
- Weight, diet, and physical activity-related beliefs and practices among pregnant and postpartum Latino women: the role of social supportPamela L Thornton
Institute for Social Research, University of Michigan, Ann Arbor, Michigan 48106, USA
Matern Child Health J 10:95-104. 2006..The objective of this study was to investigate the influence of social support on weight, diet, and physical activity-related beliefs and behaviors among pregnant and postpartum Latinas...
- Genome-wide linkage scan for the metabolic syndrome: the GENNID studyKaren L Edwards
Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington, USA
Obesity (Silver Spring) 16:1596-601. 2008..1-2q13) in the white sample and on chromosome 3 (3q26.1-3q29) in the Mexican-American sample. In conclusion, the results suggest that several regions harbor MetS susceptibility genes and that heterogeneity may exist across groups...
- Combination immunotherapy with a CpG oligonucleotide (1018 ISS) and rituximab in patients with non-Hodgkin lymphoma: increased interferon-alpha/beta-inducible gene expression, without significant toxicityJonathan W Friedberg
James P Wilmot Cancer Center, University of Rochester, Rochester, NY 14642, USA
Blood 105:489-95. 2005..CpG treatment resulted in no significant toxicity, providing rationale for further testing of this exciting combination immunotherapy approach to NHL...
- Guanosine triphosphate acts as a cofactor to promote assembly of initial P-element transposase-DNA synaptic complexesMei Tang
Division of Genetics, Genomics and Development, Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California, Berkeley, California 94720, USA
Genes Dev 19:1422-5. 2005..This initial complex then randomly and independently cleaves each P-element end. These data show that GTP acts to promote protein-DNA assembly, and may explain why P-element excision often leads to unidirectional deletions...
- Integrated Instrumentation for ProteomicsHelen Kim; Fiscal Year: 2002....