S R Khan

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi request reprint Increased expression of monocyte chemoattractant protein-1 (MCP-1) by renal epithelial cells in culture on exposure to calcium oxalate, phosphate and uric acid crystals
    Tohru Umekawa
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610 0275, USA
    Nephrol Dial Transplant 18:664-9. 2003
  2. pmc Association of Randall plaque with collagen fibers and membrane vesicles
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, Center for the Study of Lithiasis, College of Medicine, University of Florida, Gainesville, Florida 32601, USA
    J Urol 187:1094-100. 2012
  3. pmc Histological aspects of the "fixed-particle" model of stone formation: animal studies
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Investigation, College of Medicine, University of Florida, Gainesville, FL, USA
    Urolithiasis . 2016
  4. pmc Unified theory on the pathogenesis of Randall's plaques and plugs
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA
    Urolithiasis 43:109-23. 2015
  5. pmc NADPH oxidase as a therapeutic target for oxalate induced injury in kidneys
    Sunil Joshi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Oxid Med Cell Longev 2013:462361. 2013
  6. pmc Apocynin-treatment reverses hyperoxaluria induced changes in NADPH oxidase system expression in rat kidneys: a transcriptional study
    Sunil Joshi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida, United States of America
    PLoS ONE 7:e47738. 2012
  7. doi request reprint Reactive oxygen species as the molecular modulators of calcium oxalate kidney stone formation: evidence from clinical and experimental investigations
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Urol 189:803-11. 2013
  8. ncbi request reprint Stress oxidative: nephrolithiasis and chronic kidney diseases
    S R Khan
    Department of Pathology, University of Florida, Gainesville, FL, USA
    Minerva Med 104:23-30. 2013
  9. doi request reprint Is oxidative stress, a link between nephrolithiasis and obesity, hypertension, diabetes, chronic kidney disease, metabolic syndrome?
    Saeed R Khan
    Department of Pathology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Urol Res 40:95-112. 2012
  10. pmc Antiurolithic activity of Origanum vulgare is mediated through multiple pathways
    Aslam Khan
    Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan
    BMC Complement Altern Med 11:96. 2011

Research Grants

Collaborators

  • Benjamin K Canales
  • Yakov I Rabinovich
  • Wei Wang
  • Aslam Khan
  • N Chegini
  • Manoj Monga
  • Dirk J Kok
  • Daniel R Talham
  • V Butterweck
  • AMMON PECK
  • Shizuka Iida
  • Sivagnanam Thamilselvan
  • John Isaacs
  • F Atmani
  • Tohru Umekawa
  • Sunil Joshi
  • Karen Byer
  • Laurie B Gower
  • Carla Escobar
  • Pouran Habibzadegah-Tari
  • Fairland F Amos
  • Srinivas K Kumar
  • Karen J Byer
  • Samuel R Denmeade
  • P Vanachayangkul
  • Jian Zuo
  • Ravi Kumar Anchoori
  • Hirotsugu Uemura
  • Aneta Modzelewska
  • Jasjit S Grewal
  • Fernando C Delvecchio
  • Hallur Gurulingappa
  • Kinue Aihara
  • Kimberly G Christmas
  • P A Glenton
  • Benjamin T Saylor
  • J A Gokhale
  • M T Moriyama
  • J M Fasano
  • N Chow
  • Patricia A Glenton
  • Rajendra Kumar
  • Lijun Dai
  • Manuel Hidalgo
  • Madeleine Susanne Quirine Kortenhorst
  • Paul J Van Diest
  • Ernest Hamel
  • Gurulingappa Hallur
  • Taradas Sarkar
  • Hassan Khaskheli
  • Ruoli Bai
  • Simon A Williams
  • Masanori Iguchi
  • Karen G Byer
  • Jeng Y Tsai
  • Zaiquan Li
  • Glenn M Preminger
  • Pei Zhong
  • Ricardo M Brizuela
  • Surojit Sur
  • Bert Vogelstein
  • Takashi Kurita
  • Kenneth W Kinzler
  • Yuji Hatanaka
  • Phillip Buckhalts
  • Samuel Janssen
  • Elizabeth S Garrett
  • Carsten M Jakobsen
  • S Brogger Christensen
  • Hans Lilja
  • Hassan El-Shall

Detail Information

Publications64

  1. ncbi request reprint Increased expression of monocyte chemoattractant protein-1 (MCP-1) by renal epithelial cells in culture on exposure to calcium oxalate, phosphate and uric acid crystals
    Tohru Umekawa
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610 0275, USA
    Nephrol Dial Transplant 18:664-9. 2003
    ..We investigated the expression of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein by NRK52E rat renal tubular epithelial cells exposed to calcium oxalate (CaOx), brushite (Br, a calcium phosphate) and uric acid (UA) crystals...
  2. pmc Association of Randall plaque with collagen fibers and membrane vesicles
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, Center for the Study of Lithiasis, College of Medicine, University of Florida, Gainesville, Florida 32601, USA
    J Urol 187:1094-100. 2012
    ..To verify our hypothesis we critically examined renal papillary tissue from patients with stones...
  3. pmc Histological aspects of the "fixed-particle" model of stone formation: animal studies
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Investigation, College of Medicine, University of Florida, Gainesville, FL, USA
    Urolithiasis . 2016
    ..Thus, fixation appears to be either through the formation of Randall's plugs, crystal plugs clogging the openings of the ducts of Bellini or sub-epithelial crystal deposits, and the Randall's plaques...
  4. pmc Unified theory on the pathogenesis of Randall's plaques and plugs
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA
    Urolithiasis 43:109-23. 2015
    ....
  5. pmc NADPH oxidase as a therapeutic target for oxalate induced injury in kidneys
    Sunil Joshi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Oxid Med Cell Longev 2013:462361. 2013
    ....
  6. pmc Apocynin-treatment reverses hyperoxaluria induced changes in NADPH oxidase system expression in rat kidneys: a transcriptional study
    Sunil Joshi
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida, United States of America
    PLoS ONE 7:e47738. 2012
    ....
  7. doi request reprint Reactive oxygen species as the molecular modulators of calcium oxalate kidney stone formation: evidence from clinical and experimental investigations
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Urol 189:803-11. 2013
    ..Plaque formation elsewhere in the body is triggered by reactive oxygen species and oxidative stress. This review explores possible reactive oxygen species involvement in plaque formation and calcium oxalate nephrolithiasis...
  8. ncbi request reprint Stress oxidative: nephrolithiasis and chronic kidney diseases
    S R Khan
    Department of Pathology, University of Florida, Gainesville, FL, USA
    Minerva Med 104:23-30. 2013
    ..On the other hand OS produced during or as a result of nephrolithiasis may promote hypertension or diabetic nephropathy...
  9. doi request reprint Is oxidative stress, a link between nephrolithiasis and obesity, hypertension, diabetes, chronic kidney disease, metabolic syndrome?
    Saeed R Khan
    Department of Pathology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL 32610, USA
    Urol Res 40:95-112. 2012
    ..Renal disorders which lead to OS appear to be a continuum. Stress produced by one disorder may trigger the other under the right circumstances...
  10. pmc Antiurolithic activity of Origanum vulgare is mediated through multiple pathways
    Aslam Khan
    Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan
    BMC Complement Altern Med 11:96. 2011
    ..Origanum vulgare Linn has traditionally been used in the treatment of urolithiasis. Therefore, we investigated the crude extract of Origanum vulgare for possible antiurolithic effect, to rationalize its medicinal use...
  11. ncbi request reprint Dietary oxalate and calcium oxalate nephrolithiasis
    Saeed R Khan
    Department of Pathology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida 32610 0275, USA
    J Urol 178:2191-6. 2007
    ..We hypothesized that a cutback in dietary oxalate would lead to a decrease in the urinary excretion of oxalate and decreased stone recurrence. We tested the hypothesis in an animal model of calcium oxalate nephrolithiasis...
  12. ncbi request reprint Modeling of hyperoxaluric calcium oxalate nephrolithiasis: experimental induction of hyperoxaluria by hydroxy-L-proline
    S R Khan
    Department of Pathology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida 32610 0275, USA
    Kidney Int 70:914-23. 2006
    ..Thus, HLP-induced hyperoxaluria alone can induce CaOx nephrolithiasis in rats...
  13. ncbi request reprint Stone research on the bench: where we are and where we are going
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, USA
    J Endourol 16:413-6. 2002
  14. ncbi request reprint Renal tubular damage/dysfunction: key to the formation of kidney stones
    Saeed R Khan
    Department of Pathology and Center for the Study of Lithiasis, College of Medicine, University of Florida, Gainesville, FL, USA
    Urol Res 34:86-91. 2006
    ..The former will affect crystallization in the urine while the latter may cause precipitation in the interstitium and development of Randall's plaques...
  15. pmc Nephrocalcinosis in animal models with and without stones
    Saeed R Khan
    Department of Pathology, Center for the Study of Lithiasis, College of Medicine, University of Florida, Gainesville, FL, USA
    Urol Res 38:429-38. 2010
    ..Structural features of the renal tubules also play a role in crystal retention. The crystals plugging the terminal collecting ducts when exposed to the metastable pelvic urine may promote the formation of stone...
  16. pmc Calcium oxalate crystal deposition in kidneys of hypercalciuric mice with disrupted type IIa sodium-phosphate cotransporter
    Saeed R Khan
    Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA
    Am J Physiol Renal Physiol 294:F1109-15. 2008
    ..The absence of CaOx deposition in the B6 mice despite extreme hyperoxaluria also signifies the importance of both calcium and oxalate in the development of CaOx nephrolithiasis...
  17. pmc Genetic basis of renal cellular dysfunction and the formation of kidney stones
    Saeed R Khan
    Department of Pathology and Center for the Study of Lithiasis and Pathological Calcification, University of Florida, Gainesville, FL, USA
    Urol Res 37:169-80. 2009
    ..Insufficient or ineffective crystallization modulators such as osteopontin, Tamm-Horsfall protein, bikunin, etc. are most likely produced by the impaired cells...
  18. pmc Temporal changes in the expression of mRNA of NADPH oxidase subunits in renal epithelial cells exposed to oxalate or calcium oxalate crystals
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA
    Nephrol Dial Transplant 26:1778-85. 2011
    ..We have hypothesized that Ox and CaOx crystals activate NADPH oxidase through upregulation of its various subunits...
  19. pmc Experimentally induced hyperoxaluria in MCP-1 null mice
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Investigation, College of Medicine, University of Florida, Box 100275, Gainesville, FL 32610, USA
    Urol Res 39:253-8. 2011
    ....
  20. ncbi request reprint Crystal/cell interaction and nephrolithiasis
    Saeed R Khan
    Department of Pathology and Department of Urology College of Medicine, University of Florida, Gainesville, Florida 32610 0275, USA
    Arch Ital Urol Androl 83:1-5. 2011
    ..Inflammatory response may be responsible for Randall's plaques ulceration to the renal papillary surface...
  21. pmc Ultrastructural investigation of crystal deposits in Npt2a knockout mice: are they similar to human Randall's plaques?
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, Center for the Study of Lithiasis, University of Florida, Gainesville, Florida 32610 0275, USA
    J Urol 186:1107-13. 2011
    ..To determine whether this mouse is suitable for Randall's plaque investigations we chronologically studied Ca phosphate deposit sites, structure and composition...
  22. ncbi request reprint Hyperoxaluria-induced oxidative stress and antioxidants for renal protection
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, Florida 32610, USA
    Urol Res 33:349-57. 2005
    ....
  23. ncbi request reprint Role of renal epithelial cells in the initiation of calcium oxalate stones
    Saeed R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville 32610 0275, USA
    Nephron Exp Nephrol 98:e55-60. 2004
    ..Extracellular environment changes from one that inhibits crystal nucleation, growth, aggregation and retention to that, which promotes these processes...
  24. ncbi request reprint Nephrolithiasis: a consequence of renal epithelial cell exposure to oxalate and calcium oxalate crystals
    S R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610 0275, USA
    Mol Urol 4:305-12. 2000
    ..Formation of calcium oxalate (CaOx) kidney stones was investigated using three approaches...
  25. ncbi request reprint Crystal-induced inflammation of the kidneys: results from human studies, animal models, and tissue-culture studies
    Saeed R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville, FL 32610 0275, USA
    Clin Exp Nephrol 8:75-88. 2004
    ..Urate crystals also stimulate the macrophages to produce MMPs...
  26. ncbi request reprint Presence of lipids in urine, crystals and stones: implications for the formation of kidney stones
    Saeed R Khan
    Department of Pathology, University of Florida, Gainesville, Florida 32610, USA
    Kidney Int 62:2062-72. 2002
    ..We propose that membrane lipids also promote the formation of calcium oxalate (CaOx) and calcium phosphate (CaP) containing kidney stones, and become a part of their stone matrix...
  27. ncbi request reprint Modulators of urinary stone formation
    Saeed R Khan
    Department of Pathology, University of Florida, Gainesville, Florida, USA
    Front Biosci 9:1450-82. 2004
    ..In addition, macrophages surround the CaOx crystals present in the renal interstitium. These observations indicate a close relationship between inflammation and nephrolithiasis...
  28. ncbi request reprint Expression of inter-alpha inhibitor related proteins in kidneys and urine of hyperoxaluric rats
    M T Moriyama
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida, USA
    J Urol 165:1687-92. 2001
    ..Results indicate that kidneys respond to nephrolithic challenges by producing proteins that inhibit crystal formation and retention...
  29. ncbi request reprint Crystal-cell interaction and apoptosis in oxalate-associated injury of renal epithelial cells
    S R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville 32610, USA
    J Am Soc Nephrol 10:S457-63. 1999
    ..However, these cells also showed some necrotic changes such as loss of plasma membrane integrity and release of lactate dehydrogenase, indicating that the apoptotic process was interrupted...
  30. ncbi request reprint Aggregation and dispersion characteristics of calcium oxalate monohydrate: effect of urinary species
    Kimberly G Christmas
    Department of Materials Science and Engineering, University of Florida, Gainesville 32611, USA
    J Colloid Interface Sci 256:168-74. 2002
    ....
  31. ncbi request reprint Calcium phosphate-induced renal epithelial injury and stone formation: involvement of reactive oxygen species
    Kinue Aihara
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    Kidney Int 64:1283-91. 2003
    ..We hypothesized that CaP can also injure the renal epithelial cells...
  32. ncbi request reprint Reactive oxygen species mediated calcium oxalate crystal-induced expression of MCP-1 in HK-2 cells
    Pouran Habibzadegah-Tari
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, 100275, Gainesville, FL 32610 0275, USA
    Urol Res 34:26-36. 2006
    ..Based on these and other data, antioxidant therapy and blockade of rennin-angiotensin system may prove beneficial for the prevention of end stage renal disease caused by hyperoxaluria and CaOx crystal deposition...
  33. ncbi request reprint Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium
    Karen Byer
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, Florida 32610 0275, USA
    J Urol 173:640-6. 2005
    ..Since citrate increases cellular reduced nicotinamide adenine dinucleotide phosphate and glutathione (GSH), we hypothesized that exogenously administered citrate should act as an antioxidant and protect cells from oxalate induced injury...
  34. ncbi request reprint Oxalate ions and calcium oxalate crystals stimulate MCP-1 expression by renal epithelial cells
    Tohru Umekawa
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida 32610 0275, USA
    Kidney Int 61:105-12. 2002
    ..To test this hypothesis, the effects of COM and OX on the expression of MCP-1 mRNA and protein by NRK52E rat renal tubular cells were investigated...
  35. pmc Prevention of renal crystal deposition by an extract of Ammi visnaga L. and its constituents khellin and visnagin in hyperoxaluric rats
    P Vanachayangkul
    Department of Pharmaceutics, College of Pharmacy, University of Florida, PO Box 100494, Gainesville, FL 32610, USA
    Urol Res 39:189-95. 2011
    ....
  36. doi request reprint Mineralogical signatures of stone formation mechanisms
    Laurie B Gower
    Department of Materials Science and Engineering, University of Florida, Gainesville, FL, USA
    Urol Res 38:281-92. 2010
    ..Through the use of in vitro model systems, the mechanisms involved in the formation of these crystallographic features may be resolved, enhancing our understanding of the potential role(s) that proteins play in stone formation...
  37. doi request reprint Herbal medicines in the management of urolithiasis: alternative or complementary?
    Veronika Butterweck
    College of Pharmacy, Department of Pharmaceutics, University of Florida, Gainesville, Florida 32610, USA
    Planta Med 75:1095-103. 2009
    ..The present review therefore critically evaluates the potential usefulness of herbal medicines in the management of urolithiasis...
  38. ncbi request reprint Diphenyleneiodium (DPI) reduces oxalate ion- and calcium oxalate monohydrate and brushite crystal-induced upregulation of MCP-1 in NRK 52E cells
    Tohru Umekawa
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Box 100275, Gainesville, FL 32610 0275, USA
    Nephrol Dial Transplant 20:870-8. 2005
    ..The upregulation was mediated by reactive oxygen species (ROS). This study was performed to investigate whether NADPH oxidase is involved in ROS production...
  39. doi request reprint Direct AFM measurements of adhesion forces between calcium oxalate monohydrate and kidney epithelial cells in the presence of Ca2+ and Mg2+ ions
    Yakov I Rabinovich
    Particle Engineering Research Center, 205 PERC Bldg, Bldg 746, University of Florida, Gainesville, FL 32611 6135, USA
    J Colloid Interface Sci 325:594-601. 2008
    ..This probability is calculated taking into account the adhesion force, F(ad), and hydrodynamic driving force of the flow. This probability reflects the opportunity of the small aggregates to grow and form the kidney stones...
  40. ncbi request reprint Role of lipids in urinary stones: studies of calcium oxalate precipitation at phospholipid langmuir monolayers
    Daniel R Talham
    Department of Chemistry, University of Florida, Gainesville, Florida 32611 7200, USA
    Langmuir 22:2450-6. 2006
    ..The influences of the chemical identity of the lipid headgroup, the organization of the monolayer, and the presence of heterogeneities and phase boundaries within the monolayer have been explored...
  41. ncbi request reprint Adhesion force between calcium oxalate monohydrate crystal and kidney epithelial cells and possible relevance for kidney stone formation
    Yakov I Rabinovich
    University of Florida, PO Box 6135, Gainesville, FL 32611 6135, USA
    J Colloid Interface Sci 300:131-40. 2006
    ..e., does not have the viscous origin. Evaluation of the adhesion energy shows that this force should be related to the ionic or hydrogen bonds of samples...
  42. ncbi request reprint Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model
    J A Gokhale
    Department of Pathology, University of Florida, Gainesville, USA
    J Urol 166:1492-7. 2001
    ....
  43. ncbi request reprint Intratubular crystallization of calcium oxalate in the presence of membrane vesicles: an in vitro study
    J M Fasano
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610-0275, USA
    Kidney Int 59:169-78. 2001
    ..A high crystallization rate in CDs would promote rapid crystal growth and aggregation, resulting in crystal retention within the kidneys and development of nephrolithiasis...
  44. ncbi request reprint Expression of osteopontin in rat kidneys: induction during ethylene glycol induced calcium oxalate nephrolithiasis
    Saeed R Khan
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida, USA
    J Urol 168:1173-81. 2002
    ....
  45. ncbi request reprint Naturally produced crystals obtained from kidney stones are less injurious to renal tubular epithelial cells than synthetic crystals
    Carla Escobar
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida, USA
    BJU Int 100:891-7. 2007
    ..To determine the differences in cell responses to synthetic and biological crystals of calcium oxalate (CaOx) and brushite..
  46. pmc Mechanism of formation of concentrically laminated spherules: implication to Randall's plaque and stone formation
    Fairland F Amos
    Department of Materials Science and Engineering, University of Florida, Gainesville, FL 32611, USA
    Urol Res 37:11-7. 2009
    ....
  47. pmc Experimental induction of calcium oxalate nephrolithiasis in mice
    Saeed R Khan
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA
    J Urol 184:1189-96. 2010
    ..To provide evidence we induced hyperoxaluria by administering hyperoxaluria inducing agents in normocalciuric and hypercalciuric mice, and investigating various aspects of nephrolithiasis...
  48. pmc Apatite induced renal epithelial injury: insight into the pathogenesis of kidney stones
    Carla Escobar
    Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610, USA
    J Urol 180:379-87. 2008
    ..We investigated the effect of renal epithelial exposure to hydroxyapatite crystals in vitro to develop an insight into the pathogenesis of kidney stones...
  49. ncbi request reprint Oxalate induced expression of monocyte chemoattractant protein-1 (MCP-1) in HK-2 cells involves reactive oxygen species
    Pouran Habibzadegah-Tari
    Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Box 100275 Gainesville, Florida 32610 0275, USA
    Urol Res 33:440-7. 2005
    ..To our knowledge, this is the first report of MCP-1 expression and its upregulation by oxalate exposure in HK-2 cells...
  50. pmc Effect of NADPH oxidase inhibition on the expression of kidney injury molecule and calcium oxalate crystal deposition in hydroxy-L-proline-induced hyperoxaluria in the male Sprague-Dawley rats
    Jian Zuo
    Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA
    Nephrol Dial Transplant 26:1785-96. 2011
    ..We have proposed that oxalate (Ox)- and CaOx crystal-induced injury is most likely caused by reactive oxygen species (ROS) produced by activation of membrane nicotinamide adenine dinucleotide phosphate (NADPH) oxidase...
  51. ncbi request reprint Quantification of proteins extracted from calcium oxalate and calcium phosphate crystals induced in vitro in the urine of healthy controls and stone-forming patients
    Fouad Atmani
    Laboratory of Cellular Physiology and Pharmacology, Department of Biology, Faculty of Sciences, University Mohammed the First, Oujda, Morocco
    Urol Int 68:54-9. 2002
    ..We concluded that a subset of urinary proteins is present in crystal matrix. Among them, albumin seems to play a crucial role in kidney stone formation...
  52. ncbi request reprint Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants
    Sivagnanam Thamilselvan
    Department of Urology 2D, Vattikuti Urology Institute and Henry Ford Health Sciences Center, One Ford Place, Detroit, MI 48202, USA
    Urol Res 31:3-9. 2003
    ..Antioxidant administration may prevent calcium oxalate nucleation and retention in the renal tubules by preventing oxalate mediated peroxidative injury...
  53. ncbi request reprint Citrate and vitamin E blunt the shock wave-induced free radical surge in an in vitro cell culture model
    Fernando C Delvecchio
    Comprehensive Kidney Stone Center, Division of Urology, Department of Surgery, Duke University Medical Center, 1572D White Zone, DUMC 3167, Durham, NC 27710, USA
    Urol Res 33:448-52. 2005
    ..Further studies are warranted to clinically test this hypothesis...
  54. ncbi request reprint Protective role of heparin/heparan sulfate on oxalate-induced changes in cell morphology and intracellular Ca2+
    Shizuka Iida
    Department of Urology, Kurume University School of Medicine, 67 Asahi Machi, Kurume 830 0011, Japan
    Urol Res 31:198-206. 2003
    ..Heparin and HS, which are known as potent inhibitors of CaOx crystallization, may also prevent oxalate-induced cell changes by stabilizing the cytosolic Ca2+ level...
  55. ncbi request reprint Oxalate ions and calcium oxalate crystal-induced up-regulation of osteopontin and monocyte chemoattractant protein-1 in renal fibroblasts
    Tohru Umekawa
    Department of Urology, Kinki University, School of Medicine, Osaka, Japan
    BJU Int 98:656-60. 2006
    ....
  56. ncbi request reprint Effect of angiotensin II receptor blockage on osteopontin expression and calcium oxalate crystal deposition in rat kidneys
    Tohru Umekawa
    Department of Urology, Kinki University School of Medicine, Osaka, Japan
    J Am Soc Nephrol 15:635-44. 2004
    ..These results show for the first time that oxalate can activate the renal renin-angiotensin system and that oxalate-induced upregulation of OPN is in part mediated via renal renin-angiotensin system...
  57. pmc Novel microtubule-interacting phenoxy pyridine and phenyl sulfanyl pyridine analogues for cancer therapy
    Ravi Kumar Anchoori
    Division of Chemical Therapeutics, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    J Med Chem 51:5953-7. 2008
    ..Taken together, our results show that these novel microtubule inhibitors have promising anticancer activity and can be potentially used to overcome paclitaxel resistance in the clinical setting...
  58. ncbi request reprint Re: Dietary oxalate loads and renal oxalate handling. R. P. Holmes, W. T. Ambrosius and D. G. Assimos
    Saeed R Khan
    J Urol 175:1576; author reply 1576-7. 2006
  59. ncbi request reprint Synthesis and evaluation of aminophosphinic acid derivatives as inhibitors of renal dipeptidase
    Hallur Gurulingappa
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Bioorg Med Chem Lett 14:3531-3. 2004
    ..Compounds 3a and 3c in which the phenyl ring was para substituted with F and Br and olefin with Z geometry, showed better inhibitory activity against RDP enzyme (IC50 = 5-6 nM)...
  60. pmc Oxalate-inducible AMBP gene and its regulatory mechanism in renal tubular epithelial cells
    Jasjit S Grewal
    Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610 0275, USA
    Biochem J 387:609-16. 2005
    ..In situ hybridization and immunocytochemical studies showed that the expression of this gene in kidney was mainly restricted to cells lining the renal tubular system...
  61. ncbi request reprint Modulating paclitaxel bioavailability for targeting prostate cancer
    Srinivas K Kumar
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins School of Medicine, 1650 Orleans Street, Baltimore, MD 21231, USA
    Bioorg Med Chem 15:4973-84. 2007
    ....
  62. ncbi request reprint Bis(pivaloyloxymethyl) thymidine 5'-phosphate is a cell membrane-permeable precursor of thymidine 5'-phosphate in thymidine kinase deficient CCRF CEM cells
    Saeed R Khan
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Biochem Pharmacol 69:1307-13. 2005
    ..In contrast, CEM tk- incorporated radioactivity from POM(2)-dTMP into acid insoluble residue at a rate 59% that of CEM. These results demonstrate that POM(2)-dTMP can penetrate into cells and serve as a source of dTMP...
  63. ncbi request reprint Prostate-specific antigen-activated thapsigargin prodrug as targeted therapy for prostate cancer
    Samuel R Denmeade
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    J Natl Cancer Inst 95:990-1000. 2003
    ..e., G0-arrested) cells. However, thapsigargin's mechanism of action indicates that it is unlikely to be selective for cancer cells or prostate cells...
  64. ncbi request reprint Sesquiterpenes: natural products that decrease cancer growth
    Aneta Modzelewska
    The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    Curr Med Chem Anticancer Agents 5:477-99. 2005
    ..In this review, we provide a current overview of sesquiterpenoids that have potential as anticancer agents...

Research Grants2

  1. TISSUE-CRYSTAL INTERACTION CA OXALATE NEPHROLITHIASIS
    Saeed Khan; Fiscal Year: 2003
    ..We can follow various events of nephrolithiasis in the animal model and cats explore the outcome of renal epithelial cell exposure to oxalate and calcium oxalate crystals in the cell cultures. ..
  2. CaP, crystal responsible for idiopathic nephrolithasis
    Saeed Khan; Fiscal Year: 2008
    ..Type density and size must conform to limits and specifications provided in the PHS 398Instructions. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..