- A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivityDaniel G Isom
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
Proteins 79:1034-47. 2011..coli ribose-binding protein engineered to contain single, protected cysteines. These straightforward, information-rich experiments are likely to find applications in protein engineering and functional genomics...
- Picomole-scale characterization of protein stability and function by quantitative cysteine reactivityDaniel G Isom
Department of Biochemistry, Duke University, DUMC Box 3711, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 107:4908-13. 2010..We anticipate that this technique will have broad applications in high-throughput protein engineering experiments and functional genomics...