Nina Isoherranen

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Substrate specificity and ligand interactions of CYP26A1, the human liver retinoic acid hydroxylase
    Jayne E Thatcher
    Department of Pharmaceutics, Box 357610, University of Washington, Seattle, WA 98195, USA
    Mol Pharmacol 80:228-39. 2011
  2. ncbi request reprint The influence of CYP3A5 expression on the extent of hepatic CYP3A inhibition is substrate-dependent: an in vitro-in vivo evaluation
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington, USA
    Drug Metab Dispos 36:146-54. 2008
  3. pmc Comparison of the function and expression of CYP26A1 and CYP26B1, the two retinoic acid hydroxylases
    Ariel R Topletz
    Department of Pharmaceutics, University of Washington, Seattle, United States
    Biochem Pharmacol 83:149-63. 2012
  4. doi request reprint Quantitative prediction of CYP2B6 induction by estradiol during pregnancy: potential explanation for increased methadone clearance during pregnancy
    Leslie J Dickmann
    Department of Pharmacokineticsand Drug Metabolism, Amgen
    Drug Metab Dispos 41:270-4. 2013
  5. pmc Importance of multi-p450 inhibition in drug-drug interactions: evaluation of incidence, inhibition magnitude, and prediction from in vitro data
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Box 357610, Seattle, WA 98195, USA
    Chem Res Toxicol 25:2285-300. 2012
  6. pmc Drug Metabolism and Transport During Pregnancy: How Does Drug Disposition Change during Pregnancy and What Are the Mechanisms that Cause Such Changes?
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195 7610
    Drug Metab Dispos 41:256-62. 2013
  7. pmc Qualitative analysis of the role of metabolites in inhibitory drug-drug interactions: literature evaluation based on the metabolism and transport drug interaction database
    Nina Isoherranen
    Department of Pharmaceutics, University of Washington, P O Box 357610, Seattle, Washington 98195, USA
    Chem Res Toxicol 22:294-8. 2009
  8. pmc Expression and functional characterization of cytochrome P450 26A1, a retinoic acid hydroxylase
    Justin D Lutz
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, USA
    Biochem Pharmacol 77:258-68. 2009
  9. pmc Stereoselective inhibition of CYP2C19 and CYP3A4 by fluoxetine and its metabolite: implications for risk assessment of multiple time-dependent inhibitor systems
    Justin D Lutz
    Department of Pharmaceutics J D L, N I and Department of Medicinal Chemistry B M V, K N B, W L N, K L K, School of Pharmacy, University of Washington, Seattle, Washington
    Drug Metab Dispos 41:2056-65. 2013
  10. pmc Risk assessment of mechanism-based inactivation in drug-drug interactions
    Yasushi Fujioka
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA
    Drug Metab Dispos 40:1653-7. 2012

Collaborators

Detail Information

Publications53

  1. pmc Substrate specificity and ligand interactions of CYP26A1, the human liver retinoic acid hydroxylase
    Jayne E Thatcher
    Department of Pharmaceutics, Box 357610, University of Washington, Seattle, WA 98195, USA
    Mol Pharmacol 80:228-39. 2011
    ..7, 4.2, and 8.6 μM, respectively. These data demonstrate that CYP26A1 has high ligand selectivity but accepts structurally related nuclear receptor agonists as inhibitors...
  2. ncbi request reprint The influence of CYP3A5 expression on the extent of hepatic CYP3A inhibition is substrate-dependent: an in vitro-in vivo evaluation
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington, USA
    Drug Metab Dispos 36:146-54. 2008
    ..In conclusion, the effect of CYP3A5 on hepatic CYP3A-mediated inhibitory drug-drug interactions is substrate-dependent, and HLM, rather than rCYP3A, are the preferred in vitro system for predicting these interactions in vivo...
  3. pmc Comparison of the function and expression of CYP26A1 and CYP26B1, the two retinoic acid hydroxylases
    Ariel R Topletz
    Department of Pharmaceutics, University of Washington, Seattle, United States
    Biochem Pharmacol 83:149-63. 2012
    ..CYP26A1 has higher catalytic activity than CYP26B1 and seems to be responsible for metabolism of atRA in tissues that function as a barrier for atRA exposure...
  4. doi request reprint Quantitative prediction of CYP2B6 induction by estradiol during pregnancy: potential explanation for increased methadone clearance during pregnancy
    Leslie J Dickmann
    Department of Pharmacokineticsand Drug Metabolism, Amgen
    Drug Metab Dispos 41:270-4. 2013
    ..This could in part explain the observed increase in methadone clearance during pregnancy...
  5. pmc Importance of multi-p450 inhibition in drug-drug interactions: evaluation of incidence, inhibition magnitude, and prediction from in vitro data
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Box 357610, Seattle, WA 98195, USA
    Chem Res Toxicol 25:2285-300. 2012
    ..The results of this study suggest that inhibition of multiple clearance pathways in vivo is clinically relevant, and the risk of DDIs with object drugs may be best evaluated in studies using multi-P450 inhibitors...
  6. pmc Drug Metabolism and Transport During Pregnancy: How Does Drug Disposition Change during Pregnancy and What Are the Mechanisms that Cause Such Changes?
    Nina Isoherranen
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195 7610
    Drug Metab Dispos 41:256-62. 2013
    ..The studies provide the impetus for a mechanism- and evidence-based approach to optimally managing drug therapies during pregnancy and improving treatment outcomes...
  7. pmc Qualitative analysis of the role of metabolites in inhibitory drug-drug interactions: literature evaluation based on the metabolism and transport drug interaction database
    Nina Isoherranen
    Department of Pharmaceutics, University of Washington, P O Box 357610, Seattle, Washington 98195, USA
    Chem Res Toxicol 22:294-8. 2009
    ....
  8. pmc Expression and functional characterization of cytochrome P450 26A1, a retinoic acid hydroxylase
    Justin D Lutz
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, USA
    Biochem Pharmacol 77:258-68. 2009
    ..These results provide a biochemical framework for CYP26A1 function and offer insight into the role of CYP26A1 as a drug target as well as in fetal development and cell cycle regulation...
  9. pmc Stereoselective inhibition of CYP2C19 and CYP3A4 by fluoxetine and its metabolite: implications for risk assessment of multiple time-dependent inhibitor systems
    Justin D Lutz
    Department of Pharmaceutics J D L, N I and Department of Medicinal Chemistry B M V, K N B, W L N, K L K, School of Pharmacy, University of Washington, Seattle, Washington
    Drug Metab Dispos 41:2056-65. 2013
    ....
  10. pmc Risk assessment of mechanism-based inactivation in drug-drug interactions
    Yasushi Fujioka
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA
    Drug Metab Dispos 40:1653-7. 2012
    ..However, the use of total C(max) led to great overprediction of DDI risk. The risk assessment using λ/k(deg) coupled with unbound C(max) can be useful for the DDI risk evaluation of MBIs in drug discovery and development...
  11. pmc Stereospecific metabolism of itraconazole by CYP3A4: dioxolane ring scission of azole antifungals
    Chi Chi Peng
    Department of Pharmaceutics, University of Washington, P O Box 357610, Seattle, Washington 98103, USA
    Drug Metab Dispos 40:426-35. 2012
    ..These data suggest that the dioxolane ring scission is a metabolic pathway for drugs that contain this moiety...
  12. pmc Effect of CYP3A5 expression on the inhibition of CYP3A-catalyzed drug metabolism: impact on modeling CYP3A-mediated drug-drug interactions
    Yoshiyuki Shirasaka
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA
    Drug Metab Dispos 41:1566-74. 2013
    ....
  13. ncbi request reprint Stereochemical aspects of itraconazole metabolism in vitro and in vivo
    Kent L Kunze
    Department of Pharmaceutics, H272 Health Sciences Building, Box 357610, University of Washington, Seattle, WA 98195, USA
    Drug Metab Dispos 34:583-90. 2006
    ..However, stereoselective elimination was diminished after multiple dosing, presumably as a result of CYP3A4 autoinhibition. In conclusion, the metabolism of ITZ is highly stereoselective in vitro and in vivo...
  14. pmc The relative importance of CYP26A1 in hepatic clearance of all-trans retinoic acid
    Jayne E Thatcher
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, United States
    Biochem Pharmacol 80:903-12. 2010
    ..07L/min using combined Supersome data) was similar to the published in vivo clearance of RA. These findings suggest that CYP26A1 is the P450 isoform that should be targeted when designing RA metabolism blocking agents...
  15. pmc Hepatic Cyp2d and Cyp26a1 mRNAs and activities are increased during mouse pregnancy
    Ariel R Topletz
    School of Pharmacy, Department of Pharmaceutics, University of Washington, Seattle, WA 98195 7610, USA
    Drug Metab Dispos 41:312-9. 2013
    ..Our findings also suggest that retinoic acid signaling in the liver is increased during pregnancy, which may have broader implications to energy homeostasis in the liver during pregnancy...
  16. doi request reprint In vitro-to-in vivo predictions of drug-drug interactions involving multiple reversible inhibitors
    Justin D Lutz
    University of Washington School of Pharmacy, Department of Pharmaceutics, Seattle, WA, USA
    Expert Opin Drug Metab Toxicol 8:449-66. 2012
    ..This review provides the framework for predicting inhibitory DDIs of multiple inhibitors with any combination of reversible inhibition mechanism...
  17. pmc Surface plasmon resonance analysis of antifungal azoles binding to CYP3A4 with kinetic resolution of multiple binding orientations
    Josh T Pearson
    Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195 7610, USA
    Biochemistry 45:6341-53. 2006
    ..Most importantly, they provide the first example of a SPR-based method for the kinetic characterization of binding of a drug to any human CYP, including mechanistic insight not available from other methods...
  18. pmc Identification of Tazarotenic Acid as the First Xenobiotic Substrate of Human Retinoic Acid Hydroxylase CYP26A1 and CYP26B1
    Robert S Foti
    Amgen Pharmacokinetics and Drug Metabolism, Seattle, Washington R S F Department of Pharmaceutics, University of Washington, Seattle, Washington N I, A Z, L J D, B R B Core Laboratory for Neuromolecular Production, Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana P D CNRS, université Nice Sophia Antipolis, Institut de Pharmacologie Moleculaire et Cellulaire, UMR 7275, Valbonne, France D D
    J Pharmacol Exp Ther 357:281-92. 2016
    ..Overall, the homology models presented herein describe the enzyme characteristics leading to the metabolism of tazarotenic acid by CYP26A1 and CYP26B1 and support a potential role for the CYP26 enzymes in the metabolism of xenobiotics. ..
  19. pmc SAR Studies of 5-Aminopyrazole-4-carboxamide Analogues as Potent and Selective Inhibitors of Toxoplasma gondii CDPK1
    Wenlin Huang
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, United States
    ACS Med Chem Lett 6:1184-1189. 2015
    ..gondii in the brain, spleen, and peritoneal fluid, and 35 given at 20 mg/kg eliminated T. gondii from the peritoneal fluid...
  20. pmc All-Trans-Retinoic Acid Enhances Mitochondrial Function in Models of Human Liver
    Sasmita Tripathy
    Departments of Pharmaceutics S T, S L M A, N I, Medicinal Chemistry J D C, D R G, and Diabetes Obesity Center for Excellence and the Department of Medicine, Division of Metabolism, Endocrinology and Nutrition C Y H, University of Washington, Seattle, Washington School of Molecular Biosciences and The Center for Reproductive Biology, Washington State University, Pullman, Washington C A H, J O, T K and School of Pharmacy, University of Maryland, Baltimore, Maryland D R G
    Mol Pharmacol 89:560-74. 2016
    ....
  21. pmc Stereoselective formation and metabolism of 4-hydroxy-retinoic Acid enantiomers by cytochrome p450 enzymes
    Jakob A Shimshoni
    Department of Pharmaceutics, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 287:42223-32. 2012
    ..The stereoselectivity observed in CYP26A1 function will aid in better understanding of the active site features of the enzyme and the disposition of biologically active retinoids...
  22. pmc Contributions of human cytochrome P450 enzymes to glyburide metabolism
    Lin Zhou
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, USA
    Biopharm Drug Dispos 31:228-42. 2010
    ..These results confirm that human CYP3A4 is the major enzyme involved in the in vitro metabolism of GLB...
  23. pmc Direct protein-protein interactions and substrate channeling between cellular retinoic acid binding proteins and CYP26B1
    Cara H Nelson
    Department of Pharmaceutics, University of Washington, Seattle, WA, USA
    FEBS Lett 590:2527-35. 2016
    ..Similar protein-protein interactions between soluble binding proteins and CYPs may be important for other lipophilic CYP substrates. ..
  24. pmc Pharmacological inhibition of ALDH1A in mice decreases all-trans retinoic acid concentrations in a tissue specific manner
    Samuel L M Arnold
    Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA
    Biochem Pharmacol 95:177-92. 2015
    ..These data demonstrate that inhibition of ALDH1A enzymes will decrease atRA concentrations in a tissue specific manner and selective ALDH1A inhibition could be used to alter atRA concentrations in select target tissues. ..
  25. pmc Identification of human UDP-glucuronosyltransferases catalyzing hepatic 1alpha,25-dihydroxyvitamin D3 conjugation
    Takanori Hashizume
    Department of Pharmaceutics, University of Washington, Seattle, WA, United States
    Biochem Pharmacol 75:1240-50. 2008
    ..If enterohepatic recycling of 1,25(OH)2D3 represents a significant component of intestinal and systemic 1,25(OH)2D3 disposition, formation of monoglucuronides by hepatic UGT1A4 constitutes an important initial step...
  26. ncbi request reprint Evidence of significant contribution from CYP3A5 to hepatic drug metabolism
    Weili Huang
    Department of Pharmaceutics, Box 357610, University of Washington, Seattle, WA 98195 7610, USA
    Drug Metab Dispos 32:1434-45. 2004
    ..We suggest that, under conditions when CYP3A5 content represents a significant fraction of the total hepatic CYP3A pool, the contribution of CYP3A5 to the clearance of some drugs may be an important source of interindividual variability...
  27. pmc A comparison of the roles of peroxisome proliferator-activated receptor and retinoic acid receptor on CYP26 regulation
    Suzanne Tay
    Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA
    Mol Pharmacol 77:218-27. 2010
    ..The fact that drugs can alter the expression of CYP26 enzymes may have toxicological and therapeutic importance...
  28. pmc Identification and Structural Characterization of Three New Metabolites of Bupropion in Humans
    Jennifer E Sager
    Departments of Pharmaceutics, Obstetrics and Gynecology, and Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States
    ACS Med Chem Lett 7:791-6. 2016
    ..In total, these metabolites represented 24% of the drug related material excreted in urine. ..
  29. pmc Induction of CYP26A1 by metabolites of retinoic acid: evidence that CYP26A1 is an important enzyme in the elimination of active retinoids
    Ariel R Topletz
    Departments of Pharmaceutics A R T, S T, J A S, N I and Medicinal Chemistry W L N, University of Washington, Seattle, Washington and Department of Pharmacokinetics and Drug Metabolism, Amgen Inc, Seattle, Washington R S F
    Mol Pharmacol 87:430-41. 2015
    ..These data support the role of CYP26A1 to clear bioactive retinoids, and suggest that the enzyme forming active 4-oxo-atRA may be important in modulating retinoid action...
  30. pmc Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations
    Samuel L Arnold
    Department of Pharmaceutics, School of Pharmacy, School of Medicine, University of Washington, Seattle, WA 98195
    J Lipid Res 56:342-57. 2015
    ..This study provides a comprehensive novel methodology to evaluate RA homeostasis in human tissues and provides insight to how the individual ALDH1A enzymes mediate RA concentrations in specific cell types. ..
  31. pmc Inhibition of CYP2C19 and CYP3A4 by omeprazole metabolites and their contribution to drug-drug interactions
    Yoshiyuki Shirasaka
    Department of Pharmaceutics, School of Pharmacy, University of Washington, University of Washington, Seattle, WA, USA
    Drug Metab Dispos 41:1414-24. 2013
    ..The results of this study show that, although metabolites contribute to in vivo DDIs, their relative abundance in circulation or logP values do not predict their contribution to in vivo DDI risk...
  32. pmc Changes in maternal liver Cyp2c and Cyp2d expression and activity during rat pregnancy
    Leslie J Dickmann
    Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA
    Biochem Pharmacol 75:1677-87. 2008
    ..These data show that pregnancy significantly alters the expression and activity of drug metabolizing enzymes in an enzyme and gestational stage specific manner. These changes are likely to have toxicological and therapeutic implications...
  33. ncbi request reprint Effect of CYP3A5 polymorphism on tacrolimus metabolic clearance in vitro
    Yang Dai
    Department of Pharmaceutics, University of Washington, Seattle, 98195 7610, USA
    Drug Metab Dispos 34:836-47. 2006
    ..These data suggest that CYP3A5 contributes significantly to the metabolic clearance of tacrolimus in the liver and kidney...
  34. pmc Development of an Orally Available and Central Nervous System (CNS) Penetrant Toxoplasma gondii Calcium-Dependent Protein Kinase 1 (TgCDPK1) Inhibitor with Minimal Human Ether-a-go-go-Related Gene (hERG) Activity for the Treatment of Toxoplasmosis
    Rama Subba Rao Vidadala
    Department of Chemistry, University of Washington, Seattle, Washington 98195, United States
    J Med Chem 59:6531-46. 2016
    ..These properties make 32 a promising lead for the development of a new antitoxoplasmosis therapy. ..
  35. pmc Levels of the retinoic acid synthesizing enzyme aldehyde dehydrogenase-1A2 are lower in testicular tissue from men with infertility
    John K Amory
    Department of Medicine, University of Washington, Seattle, Washington Electronic address
    Fertil Steril 101:960-6. 2014
    ..To determine whether decreased testicular levels of enzymes necessary for retinoic acid biosynthesis were associated with male infertility, as retinoic acid is known to be necessary for spermatogenesis...
  36. pmc Nitric oxide and interleukin-1β stimulate the proteasome-independent degradation of the retinoic acid hydroxylase CYP2C22 in primary rat hepatocytes
    Choon Myung Lee
    Department of Pharmacology, Emory University, Atlanta, Georgia C m L, B s L, E T M and Department of Pharmaceutics, University of Washington, Seattle, Washington S L A, N I
    J Pharmacol Exp Ther 348:141-52. 2014
    ..However, this degradation was insensitive to inhibitors of calpains or the canonical proteasomal or lysosomal pathways, indicating that NO-dependent degradation of CYP2C22 proceeds via a novel pathway. ..
  37. pmc The role of metabolites in predicting drug-drug interactions: focus on irreversible cytochrome P450 inhibition
    Brooke M VandenBrink
    University of Washington, Department of Medicinal Chemistry, Box 357610, Seattle, WA 98195, USA
    Curr Opin Drug Discov Devel 13:66-77. 2010
    ..Data discussed in this review suggest that circulating metabolites are more important in CYP inhibition in vivo than has been acknowledged...
  38. pmc The role of CYP26 enzymes in retinoic acid clearance
    Jayne E Thatcher
    University of Washington, Department of Pharmaceutics, School of Pharmacy, Seattle, WA 98195, USA
    Expert Opin Drug Metab Toxicol 5:875-86. 2009
    ..To further the understanding of how CYP26 enzymes contribute to the regulation of RA homeostasis, structural information of the CYP26s, commercially available recombinant enzymes and good specific and sensitive antibodies are needed...
  39. pmc In vitro to in vivo extrapolation of the complex drug-drug interaction of bupropion and its metabolites with CYP2D6; simultaneous reversible inhibition and CYP2D6 downregulation
    Jennifer E Sager
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA
    Biochem Pharmacol . 2016
    ..This study is the first example of a clinical DDI resulting from CYP down-regulation and first demonstration of a CYP2D6 interaction resulting from transcriptional regulation...
  40. pmc Stereoselective Metabolism of Bupropion to OH-bupropion, Threohydrobupropion, Erythrohydrobupropion, and 4'-OH-bupropion in vitro
    Jennifer E Sager
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington
    Drug Metab Dispos 44:1709-19. 2016
    ..The stereoselective pharmacokinetics of bupropion were quantitatively explained by the in vitro metabolic clearances and in vivo interconversion between bupropion stereoisomers. ..
  41. pmc Impact of Sample Matrix on Accuracy of Peptide Quantification: Assessment of Calibrator and Internal Standard Selection and Method Validation
    Samuel L Arnold
    Department of Pharmaceutics, University of Washington, Health Science Building, Room H 272M, Box 357610, Seattle, Washington 98195 7610, United States
    Anal Chem 88:746-53. 2016
    ..The results demonstrate that different sample matrices have peptide, time, and matrix specific effects on protein digestion and absolute quantification. ..
  42. pmc Physiologically Based Pharmacokinetic (PBPK) Modeling and Simulation Approaches: A Systematic Review of Published Models, Applications, and Model Verification
    Jennifer E Sager
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington
    Drug Metab Dispos 43:1823-37. 2015
    ..An in-depth analysis of the model development and verification revealed a lack of consistency in model development and quality assessment practices, demonstrating a need for development of best-practice guidelines. ..
  43. pmc Therapeutic potential of the inhibition of the retinoic acid hydroxylases CYP26A1 and CYP26B1 by xenobiotics
    Cara H Nelson
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA 98195, USA
    Curr Top Med Chem 13:1402-28. 2013
    ..This review summarizes the history of development of RAMBAs, the clinical and preclinical studies with the various structural series and the available knowledge of structure activity relationships of CYP26 inhibitors...
  44. pmc A physiologically based pharmacokinetic model to predict disposition of CYP2D6 and CYP1A2 metabolized drugs in pregnant women
    Alice Ban Ke
    Department of Pharmaceutics, University of Washington, Box 357610, Seattle, WA 98195, USA
    Drug Metab Dispos 41:801-13. 2013
    ..Based on these data, it is prudent to conclude that the magnitude of hepatic CYP2D6 induction during T3 ranges from 100 to 200%. Our PBPK model can predict the disposition of CYP1A2, 2D6, and 3A drugs during pregnancy...
  45. ncbi request reprint Role of itraconazole metabolites in CYP3A4 inhibition
    Nina Isoherranen
    University of Washington, Department of Pharmaceutics, H272 Health Sciences Building, Box 357610, Seattle, WA 98195 7610, USA
    Drug Metab Dispos 32:1121-31. 2004
    ..These findings demonstrate that ITZ metabolites are as potent as or more potent CYP3A4 inhibitors than ITZ itself, and thus may contribute to the inhibition of CYP3A4 observed in vivo after ITZ dosing...
  46. pmc Rationalization and prediction of in vivo metabolite exposures: the role of metabolite kinetics, clearance predictions and in vitro parameters
    Justin D Lutz
    Department of Pharmaceutics, University of Washington, School of Pharmacy, H272 Health Science Building, Box 357610, Seattle, WA 98195 7610, USA
    Expert Opin Drug Metab Toxicol 6:1095-109. 2010
    ..However, limited tools are available to quantitatively predict their human exposures...
  47. pmc A sensitive and specific method for measurement of multiple retinoids in human serum with UHPLC-MS/MS
    Samuel L M Arnold
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, WA, USA
    J Lipid Res 53:587-98. 2012
    ..11-cisRA and 4OH-RA were not detected in human serum. The high sensitivity of the MS/MS method combined with the UHPLC separation power allowed detection of endogenous 9-cisRA and 4oxo-atRA for the first time in human serum...
  48. pmc Pregnancy decreases rat CYP1A2 activity and expression
    Alysa A Walker
    Department of Pharmaceutics, University of Washington, Seattle, Washington 98195 7610, USA
    Drug Metab Dispos 39:4-7. 2011
    ....
  49. pmc Role of Retinoic Acid-Metabolizing Cytochrome P450s, CYP26, in Inflammation and Cancer
    Faith Stevison
    Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington, USA
    Adv Pharmacol 74:373-412. 2015
    ....
  50. pmc Detection of an endogenous urinary biomarker associated with CYP2D6 activity using global metabolomics
    Jessica Tay-Sontheimer
    Department of Pharmaceutics, University of Washington, Seattle, WA, USA
    Pharmacogenomics 15:1947-62. 2014
    ..We sought to discover endogenous urinary biomarkers of human CYP2D6 activity...
  51. pmc A specific inhibitor of PfCDPK4 blocks malaria transmission: chemical-genetic validation
    Kayode K Ojo
    Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle
    J Infect Dis 209:275-84. 2014
    ..Ongoing studies include refining the compounds to improve efficacy and toxicological properties for efficient blocking of malaria transmission...
  52. pmc Prediction of relative in vivo metabolite exposure from in vitro data using two model drugs: dextromethorphan and omeprazole
    Justin D Lutz
    School of Pharmacy, Department of Pharmaceutics, University of Washington, Seattle, WA 98195 7610, USA
    Drug Metab Dispos 40:159-68. 2012
    ..This study shows that relative in vivo metabolite exposure can be predicted from in vitro data and characterization of secondary metabolism of probe metabolites is critical for interpretation of phenotypic data...
  53. pmc Suppression of spermatogenesis by bisdichloroacetyldiamines is mediated by inhibition of testicular retinoic acid biosynthesis
    John K Amory
    Department of Medicine, University of Washington School of Medicine and School of Pharmaceutics, Seattle, WA 98195, USA
    J Androl 32:111-9. 2011
    ..These findings suggest that ALDH1a2 is a promising target for the development of a reversible, nonhormonal male contraceptive...