Thomas K Hunt
Affiliation: University of California
- Copper-induced vascular endothelial growth factor expression and wound healingChandan K Sen
Laboratory of Molecular Medicine, Department of Surgery, 512 Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
Am J Physiol Heart Circ Physiol 282:H1821-7. 2002..Copper-sensitive pathways regulate key mediators of wound healing such as angiogenesis and extracellular matrix remodeling. Copper-based therapeutics represents a feasible approach to promote dermal wound healing...
- Oxygen: at the foundation of wound healing--introductionThomas K Hunt
World J Surg 28:291-3. 2004..A historical and evolutionary perspective on the role of oxygen in wound healing--from the classical physiology of oxygen in the wound to the refined concept of redox signaling--is presented...
- Regulatory role of lactate in wound repairQ Perveen Ghani
UCSF, Wound Healing Laboratory, San Francisco, California 94143 0522, USA
Methods Enzymol 381:565-75. 2004
- Hyperoxia improves microvascular perfusion in a murine wound modelAhmad Y Sheikh
Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Wound Repair Regen 13:303-8. 2005..05). Laser Doppler imaging was able to detect and quantify the increase in wound bed perfusion resulting from intermittent HBO treatments and shows promise as a noninvasive measure of angiogenesis and wound healing...
- Aerobically derived lactate stimulates revascularization and tissue repair via redox mechanismsThomas K Hunt
Wound Healing Laboratory, University of California San Francisco, San Francisco, California 94143 0522, USA
Antioxid Redox Signal 9:1115-24. 2007..Accumulated lactate, therefore, appears to convey the impression of "metabolic need" for vascularization, even in well-oxygenated and pH-neutral conditions. Lactate and oxygen together stimulate angiogenesis and matrix deposition...
- Lactate, with oxygen, incites angiogenesisThomas K Hunt
Department of Surgery, University of California Medical Center, San Francisco, CA 94143, USA
Adv Exp Med Biol 614:73-80. 2008..This paper, a mini-review, is occasioned by new data showing increased presence of VEGF and angiogenesis in an oxygenated site by adding a slow-release source of lactate into Matrigel and implanting the Matrigel subcutaneously in mice...
- Lactate stimulates endothelial cell migrationStefan Beckert
Department of Surgery, University of California, San Francisco, California, USA, and Department of General Surgery, University Hospital Tubingen, Germany
Wound Repair Regen 14:321-4. 2006..Lactate enhances VEGF production in endothelial cells, although lactate, itself, is not a chemoattractant. We conclude that the lactate-mediated increase in cellular migration is regulated by VEGF...
- Hyperoxia and angiogenesisHarriet W Hopf
Wound Healing Research Laboratory, Department of Anesthesia and Perioperative Care, University of California, San Francisco, California 94143, USA
Wound Repair Regen 13:558-64. 2005..We conclude that angiogenesis is proportional to ambient pO(2) over a wide range. This confirms the clinical impression that angiogenesis requires oxygen. Intermittent oxygen exposure can satisfy the need for oxygen in ischemic tissue...
- Using physiology to improve surgical wound outcomesChristiane Ueno
University of California, San Francisco, Wound Healing Laboratory, Departments of Anesthesia and Perioperative Care and Surgery, San Francisco, Calif 94143 0648, USA
Plast Reconstr Surg 117:59S-71S. 2006..This article reviews how knowledge of and attention to physiology can improve quality of care in both acute and chronic wounds...
- Lactate and oxygen constitute a fundamental regulatory mechanism in wound healingOdilo Trabold
Department of Surgery, University of California, San Francisco, San Francisco, California 94143, USA
Wound Repair Regen 11:504-9. 2003..The data are consistent with ADP-ribosylation effects and oxidant signaling. (WOUND REP REG 2003;11:504-509)..
- IGF-I-induced VEGF expression in HUVEC involves phosphorylation and inhibition of poly(ADP-ribose)polymeraseStefan Beckert
Department of Surgery, School of Medicine, University of California, San Francisco, 94123, USA
Biochem Biophys Res Commun 341:67-72. 2006..The IGF-I-mediated phosphorylation and inhibition of PARP represent a novel mechanism of VEGF protein expression...
- Role of ADP-ribosylation in wound repair. The contributions of Thomas K. Hunt, MDQ Perveen Ghani
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, California 94143, USA
Wound Repair Regen 11:439-44. 2003..Down-regulation of the ubiquitous protein modification reaction called ADP-ribosylation turned out to be a basic signal behind the role of lactate in wound healing...
- Development of subcutaneous wound oxygen measurement in humans: contributions of Thomas K Hunt, MDHarriet W Hopf
Department of Anesthesia and Perioperative Care, University of California, San Francisco, California 94143 0648, USA
Wound Repair Regen 11:424-30. 2003..All these observations required tissue oxygen measurement, and, in particular, translation of basic scientific observations to clinical research required a method of measuring wound oxygen tension in humans...
- Measuring oxygen in woundsHarriet W Hopf
Department of Surgery, UCSF, Wound Healing Laboratory, San Francisco, California 94143 0522, USA
Methods Enzymol 381:539-64. 2004
- The role of oxygen during fracture healingChuanyong Lu
Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, San Francisco General Hospital, University of California at San Francisco, 1001 Potrero Ave, San Francisco, CA 94110, USA
Bone 52:220-9. 2013..In conclusion, our results indicate that environmental oxygen levels affect tissue vascularization and fracture healing, and that providing oxygen when fractures are accompanied by ischemia may be beneficial...
- Methods of measuring oxygen in woundsHarriet W Hopf
Department of Anesthesia and Perioperative Care, Department of Surgery, University of California, San Francisco, CA, USA
Methods Mol Med 78:389-416. 2003
- Accuracy of a polarographic electrode at high oxygen concentrationsMark D Rollins
Bioengineering Graduate Group, University of California, San Francisco, CA, USA
Adv Exp Med Biol 510:169-73. 2003
- Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1Tatyana N Milovanova
Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104 6068, USA
Mol Cell Biol 28:6248-61. 2008..Oxidative stress from lactate metabolism by SPCs accelerated further SPC recruitment and differentiation through Trx1-mediated elevations in HIF-1 levels and the subsequent synthesis of HIF-1-dependent growth factors...
- Noninvasive assessment of wound-healing angiogenesis with contrast-enhanced MRIThomas H Helbich
Center for Pharmaceutical and Molecular Imaging, Department of Radiology, University of California, San Francisco 94143 0628, USA
Acad Radiol 9:S145-7. 2002
- Oxidant-induced vascular endothelial growth factor expression in human keratinocytes and cutaneous wound healingChandan K Sen
Laboratory of Molecular Medicine, Dorothy M Davis Heart and Lung Research Institute, Department of Surgery Center for Minimally Invasive Surgery, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
J Biol Chem 277:33284-90. 2002..Taken together, the results of this study indicate that wound healing is subject to redox control...
- Dermal wound healing is subject to redox controlSashwati Roy
Laboratory of Molecular Medicine, Department of Surgery, Comprehensive Wound Center, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA
Mol Ther 13:211-20. 2006..Taken together, this work presents the first in vivo evidence indicating that strategies to influence the redox environment of the wound site may have a bearing on healing outcomes...
- Oxygen: from the benefits of inducing VEGF expression to managing the risk of hyperbaric stressViren Patel
Laboratory of Molecular Medicine, Department of Surgery, The Ohio State University Medical Center, Columbus, OH 43210, USA
Antioxid Redox Signal 7:1377-87. 2005..This is of special concern for people with inadequate intake of dietary antioxidants. Nutritional antioxidant supplementation may offset HBO-induced oxidative stress...
- Comparison of inflammatory and systemic sources of growth factors in acute and chronic human woundsSilvia Wagner
Department of General Surgery, University Hospital Tubingen, Germany
Wound Repair Regen 11:253-60. 2003..If blood IGF-I is low, wound levels are lower. This data suggests that healing impairment due to IGF-I deficiency can be readily detected and is, at least in part, easily and safely correctable...
- On squeezing blood out of turnipsThomas K Hunt
Int J Low Extrem Wounds 4:130-1. 2005
- Significance of oxygen therapeuticsGayle M Gordillo
Wound Repair Regen 11:393; author reply 393. 2003
- Correlation of a simple direct measurement of muscle pO(2) to a clinical ischemia index and histology in a rat model of chronic severe hindlimb ischemiaRobert Paek
Division of Vascular Surgery, Department of Surgery, University of California San Francisco, USA
J Vasc Surg 36:172-9. 2002..Our aim was to develop a model of clinically relevant severe limb ischemia and correlate a simple direct measurement of muscle pO(2) to a clinical ischemia index, muscle mass, and capillary density...
- Age-dependency of insulin-like growth factors, insulin-like growth factor-binding proteins, and acid labile subunit in plasma and wounds of surgical patientsCorinna Wicke
Department of Surgery, Pediatric Endocrinology Section of the Children s Hospital, and BG Trauma Center, University of Tubingen, Tubingen, Germany
Wound Repair Regen 10:360-5. 2002..The current data adds to accumulating evidence that a decline in the IGF system in aged patients contributes to the healing deficits observed in the elderly...
- Perflubron emulsion increases subcutaneous tissue oxygen tension in ratsNoah A Rosen
Department of Surgery, Boston University Medical Center, Boston, Massachusetts, USA
Wound Repair Regen 14:55-60. 2006..0 vol%. These findings agree with those results analytically predicted based on the oxygen solubility of the perflubron emulsion...
- Supplemental oxygen and risk of surgical site infectionHarriet W Hopf
JAMA 291:1956; author reply 1958-9. 2004
- Stimulation of fibroblast proliferation by lactate-mediated oxidantsSilvia Wagner
Department of General Surgery, University Hospital Tubingen, Medical Research Center ZMF, Waldhoernlestrasse 22, 72072 Tubingen, Germany
Wound Repair Regen 12:368-73. 2004..The antioxidants catalase and mannitol abolish the inhibitory effect of high lactate. We conclude from these results that increased proliferation of cultured human fibroblasts by exogenous lactate is mediated by oxidant production...