Keli L Hippen
Affiliation: University of Minnesota
- Infusion of ex vivo expanded T regulatory cells in adults transplanted with umbilical cord blood: safety profile and detection kineticsClaudio G Brunstein
Blood and Marrow Transplant Program, University of Minnesota, Minneapolis, MN, USA
Blood 117:1061-70. 2011..These results set the stage for a definitive study of UCB Treg to determine its potency in preventing allogeneic aGVHD. This study is registered at http://www.clinicaltrials.gov as NCT00602693...
- Blocking IL-21 signaling ameliorates xenogeneic GVHD induced by human lymphocytesKeli L Hippen
Department of Pediatrics, Division of Hematology Oncology and Blood and Marrow Transplantation, University of Minnesota, Minneapolis, 55455, USA
Blood 119:619-28. 2012..Based on these findings, anti-IL-21 mAb could be considered for GVHD prevention in the clinic...
- Clinical perspectives for regulatory T cells in transplantation toleranceKeli L Hippen
University of Minnesota Cancer Center, Division of Bone Marrow Transplantation, Minneapolis, MN, USA
Semin Immunol 23:462-8. 2011..This review will summarize the clinical trials conducted to date that have employed Tregs to prevent GVHD following HSCT and discuss recent advances in Treg cellular therapy...
- Massive ex vivo expansion of human natural regulatory T cells (T(regs)) with minimal loss of in vivo functional activityKeli L Hippen
Division of Bone Marrow Transplantation, Department of Pediatrics, University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
Sci Transl Med 3:83ra41. 2011..The capability to efficiently produce donor cell banks of functional nT(regs) could transform the treatment of GVHD and autoimmunity by providing an off-the-shelf, cost-effective, and proven cellular therapy...
- Generation and large-scale expansion of human inducible regulatory T cells that suppress graft-versus-host diseaseK L Hippen
Department of Pediatrics, Division of Hematology Oncology and Blood and Marrow Transplantation, University of Minnesota Cancer Center, Minneapolis, MN, USA
Am J Transplant 11:1148-57. 2011..Most importantly, Rapa/TGFß iTregs suppress disease in a xenogeneic model of GVHD. This study opens the door for iTreg cellular therapy for human diseases...
- Umbilical cord blood regulatory T-cell expansion and functional effects of tumor necrosis factor receptor family members OX40 and 4-1BB expressed on artificial antigen-presenting cellsKeli L Hippen
University of Minnesota Cancer Center and Department of Pediatrics, Division of Bone, Blood and Marrow Transplantation, Minneapolis, USA
Blood 112:2847-57. 2008..These data offer a novel approach for UCB Treg expansion using aAPCs, including those coexpressing OX40L or 4-1BBL...
- Late immature B cells (IgMhighIgDneg) undergo a light chain receptor editing response to soluble self-antigenLina E Tze
Center for Immunology and Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
J Immunol 171:678-82. 2003..These studies suggest that the developmental window available for immature B cells to edit their Ig receptors, at least in response to certain soluble Ags, extends through the IgM(high) late immature B cell stage...
- B cell receptor basal signaling regulates antigen-induced Ig light chain rearrangementsBrian R Schram
Center for Immunology, Department of Medicine, University of Minnesota Medical School, University of Minnesota, Minneapolis, MN 55455, USA
J Immunol 180:4728-41. 2008..These findings support a model whereby Ag-induced receptor editing is inhibited by BCR basal signaling on developing B cells; BCR down-regulation removes this basal signal, thereby initiating receptor editing...
- In vivo assessment of the relative contributions of deletion, anergy, and editing to B cell self-toleranceKeli L Hippen
Departments of Medicine and Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
J Immunol 175:909-16. 2005..These data identify receptor editing as a major physiologic mechanism by which highly self-reactive B cells are tolerized to membrane and soluble self-Ags...
- Visualization of the genesis and fate of isotype-switched B cells during a primary immune responseKathryn A Pape
Department of Microbiology and Center for Immunology, University of Minnesota Medical School, MMC334, 420 Delaware St S E, Minneapolis, MN 55455, USA
J Exp Med 197:1677-87. 2003..Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment...
- miR-146b antagomir-treated human Tregs acquire increased GVHD inhibitory potencyYunjie Lu
Translational Medicine Research Center, Affiliated jiangning Hospital, and Liver Transplantation Center, First Affiliated Hospital, Nanjing Medical University, Nanjing, China Division of Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota Cancer Center, Minneapolis, MN
Blood 128:1424-35. 2016..In vitro exposure of human tTregs to miR-146b antagomirs can be exploited to improve the clinical efficacy of human adoptive tTreg transfer in a GVHD setting. ..
- Basal immunoglobulin signaling actively maintains developmental stage in immature B cellsLina E Tze
Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
PLoS Biol 3:e82. 2005..These studies identify a previously unappreciated level of plasticity in the B cell developmental program, and have important implications for our understanding of central tolerance mechanisms...
- Preclinical Testing of Antihuman CD28 Fab' Antibody in a Novel Nonhuman Primate Small Animal Rodent Model of Xenogenic Graft-Versus-Host DiseaseKeli L Hippen
1 Division of Hematology Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota, Minneapolis, MN 2 Aflac Cancer and Blood Disorders Center, Children s Healthcare of Atlanta, Emory University, Atlanta, GA 3 Ben Towne Center for Childhood Cancer Research, Seattle Children s Research Institute, Seattle WA, The University of Washington, Seattle, WA 4 Department of Pediatrics, The University of Washington, Seattle, WA 5 Fred Hutchinson Cancer Research Center, Seattle WA 6 INSERM, UMR 1064 Center for Research in Transplantation and Immunology, Nantes, France 7 ITUN, CHU Nantes, Nantes, France 8 University of Nantes, Nantes, France 9 Effimune SA, Nantes, France
Transplantation 100:2630-2639. 2016..To analyze efficacy and better understand immunological tolerance, escape mechanisms, and side effects of clinical reagents, testing of species cross-reactive human agents in large animal GVHD models is critical...
- Immune regulatory cells in umbilical cord blood: T regulatory cells and mesenchymal stromal cellsJakub Tolar
Blood and Marrow Transplant Program, Department of Pediatrics and Center for Translational Medicine, University of Minnesota, MMC 366, 420 Delaware St SE, Minneapolis, MN 55455, USA
Br J Haematol 147:200-6. 2009..More recent studies have indicated that umbilical cord blood (UCB) is a rich source of both cell types. We will review the current data on UCB-derived Tregs and MSCs and their therapeutic implications...
- Toward revision of antimicrobial therapies in hematopoietic stem cell transplantation: target the pathogens, but protect the indigenous microbiotaAlexander Khoruts
Division of Gastroenterology, Department of Medicine, Center for Immunology and Biotechnology Institute, University of Minnesota, Minneapolis, Minn Electronic address
Transl Res . 2016..In addition, we highlight possible strategies that are being developed to allow targeted antimicrobial therapy against pathogens, while minimizing the harm to indigenous microbiota...
- Attenuation of IL-7 receptor signaling is not required for allelic exclusionWynette M Will
Department of Laboratory Medicine and Pathology, The Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
J Immunol 176:3350-5. 2006..Thus, attenuation of IL-7R/STAT5 signaling is not required for allelic exclusion...
- Fatal acute lymphoblastic leukemia in mice transgenic for B cell-restricted bcl-xL and c-mycPenelope J Swanson
Department of Medicine, Cancer Center, and Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA
J Immunol 172:6684-91. 2004..Tumors in these animals target an early stage in B cell development characterized by the expression of both B lineage and stem cell genes...