TAPAS HAZRA

Summary

Affiliation: University of Texas Medical Branch
Country: USA

Publications

  1. pmc Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress
    Soumita Das
    Sealy Center for Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 282:28474-84. 2007
  2. pmc Oxidative DNA damage repair in mammalian cells: a new perspective
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of Biochemistry and Molecular Biology, 6 136 Medical Research Building, Route 1079, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 6:470-80. 2007
  3. ncbi request reprint Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions
    Tapas K Hazra
    Department of Human Biological Chemistry and Genetics, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Rte 1079, Galveston, TX 77555, USA
    J Biol Chem 277:30417-20. 2002
  4. pmc Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 99:3523-8. 2002
  5. pmc Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1
    Muralidhar L Hegde
    Department of Biochemistry and Molecular Biology, University of Texas, Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 283:27028-37. 2008
  6. ncbi request reprint The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1
    Aditi Das
    Department of Biochemistry and Molecular Biology and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 282:26591-602. 2007
  7. pmc NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells
    Aditi Das
    Department of Biochemistry and Molecular Biology, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
    DNA Repair (Amst) 5:1439-48. 2006
  8. pmc RPA physically interacts with the human DNA glycosylase NEIL1 to regulate excision of oxidative DNA base damage in primer-template structures
    Corey A Theriot
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA
    DNA Repair (Amst) 9:643-52. 2010
  9. ncbi request reprint Purification and characterization of NEIL1 and NEIL2, members of a distinct family of mammalian DNA glycosylases for repair of oxidized bases
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of HBC and G, University of Texas Medical Branch, Galveston, USA
    Methods Enzymol 408:33-48. 2006
  10. ncbi request reprint Identification of a zinc finger domain in the human NEIL2 (Nei-like-2) protein
    Aditi Das
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    J Biol Chem 279:47132-8. 2004

Research Grants

Collaborators

Detail Information

Publications39

  1. pmc Stimulation of NEIL2-mediated oxidized base excision repair via YB-1 interaction during oxidative stress
    Soumita Das
    Sealy Center for Molecular Medicine and Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 282:28474-84. 2007
    ..NEIL2-initiated base excision activity is significantly reduced in YB-1-depleted cells. YB-1 thus appears to have a novel regulatory role in NEIL2-mediated repair under oxidative stress...
  2. pmc Oxidative DNA damage repair in mammalian cells: a new perspective
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of Biochemistry and Molecular Biology, 6 136 Medical Research Building, Route 1079, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 6:470-80. 2007
    ....
  3. ncbi request reprint Identification and characterization of a novel human DNA glycosylase for repair of cytosine-derived lesions
    Tapas K Hazra
    Department of Human Biological Chemistry and Genetics, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Rte 1079, Galveston, TX 77555, USA
    J Biol Chem 277:30417-20. 2002
    ..Despite the absence of a putative nuclear localization signal, NEIL2 was predominantly localized in the nucleus. These results suggest that NEIL2 is involved in global genome repair mainly for removing oxidative products of cytosine...
  4. pmc Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 99:3523-8. 2002
    ..The tissue-specific levels of NEH1 and OGG1 mRNAs are distinct, and S phase-specific increase in NEH1 at both RNA and protein levels suggests that NEH1 is involved in replication-associated repair of oxidized bases...
  5. pmc Physical and functional interaction between human oxidized base-specific DNA glycosylase NEIL1 and flap endonuclease 1
    Muralidhar L Hegde
    Department of Biochemistry and Molecular Biology, University of Texas, Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 283:27028-37. 2008
    ..Interaction between NEIL1 and FEN-1 is essential for efficient NEIL1-initiated LP-BER. These studies strongly implicate NEIL1 in a distinct subpathway of LP-BER in replicating genomes...
  6. ncbi request reprint The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1
    Aditi Das
    Department of Biochemistry and Molecular Biology and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 282:26591-602. 2007
    ..Interestingly, WRN deficiency does not have an additive effect on in vivo damage accumulation in NEIL1 knockdown cells suggesting that WRN participates in the same repair pathway as NEIL1...
  7. pmc NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells
    Aditi Das
    Department of Biochemistry and Molecular Biology, Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
    DNA Repair (Amst) 5:1439-48. 2006
    ..The BER proteins including NEIL2, PNK, Pol beta, Lig IIIalpha and XRCC1 (but not APE1) could be isolated as a complex from human cells, competent for repair of 5-OHU in plasmid DNA...
  8. pmc RPA physically interacts with the human DNA glycosylase NEIL1 to regulate excision of oxidative DNA base damage in primer-template structures
    Corey A Theriot
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA
    DNA Repair (Amst) 9:643-52. 2010
    ..These results showing modulation of NEIL1's activity on single-stranded DNA substrate by RPA and PCNA support NEIL1's involvement in repairing the replicating genome...
  9. ncbi request reprint Purification and characterization of NEIL1 and NEIL2, members of a distinct family of mammalian DNA glycosylases for repair of oxidized bases
    Tapas K Hazra
    Sealy Center for Molecular Science and Department of HBC and G, University of Texas Medical Branch, Galveston, USA
    Methods Enzymol 408:33-48. 2006
    ....
  10. ncbi request reprint Identification of a zinc finger domain in the human NEIL2 (Nei-like-2) protein
    Aditi Das
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    J Biol Chem 279:47132-8. 2004
    ..A R310Q mutation significantly reduced the activity of NEIL2. We thereby conclude that the zinc finger motif in NEIL2 is essential for its structural integrity and enzyme activity...
  11. pmc Early steps in the DNA base excision/single-strand interruption repair pathway in mammalian cells
    Muralidhar L Hegde
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Cell Res 18:27-47. 2008
    ..The mammalian enzymes are sometimes covalently modified which may affect activity and complex formation. The focus of this review is on the early steps in mammalian BER for oxidized damage...
  12. ncbi request reprint Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2
    Hong Dou
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 278:49679-84. 2003
    ..OGG1/NTH1-independent repair of oxidized bases in the transcribed sequences supports the possibility that NEILs are preferentially involved in repair of lesions in DNA bubbles generated during transcription and/or replication...
  13. ncbi request reprint The discovery of a new family of mammalian enzymes for repair of oxidatively damaged DNA, and its physiological implications
    Tapas K Hazra
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555, USA
    Carcinogenesis 24:155-7. 2003
    ....
  14. ncbi request reprint Modulation of DNA-dependent protein kinase activity in chlorambucil-treated cells
    Attila Bacsi
    Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA
    Free Radic Biol Med 39:1650-9. 2005
    ..Data from these studies provide the first evidence that oxidative stress effects posttranslational modification and assembly of DNA-PK complex at DNA dsbs, and thereby repair of DNA dsbs...
  15. ncbi request reprint Induction of the human oxidized base-specific DNA glycosylase NEIL1 by reactive oxygen species
    Aditi Das
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
    J Biol Chem 280:35272-80. 2005
    ..Oxidative stress-induced activation of NEIL1 appears to be involved in the feedback regulation of cellular repair activity needed to handle an increase in the level of oxidative base damage...
  16. ncbi request reprint Mammalian DNA base excision repair proteins: their interactions and role in repair of oxidative DNA damage
    Tadahide Izumi
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Toxicology 193:43-65. 2003
    ..The key to understand the complete BER process is to elucidate how multiple proteins interact with one another in a coordinated process under specific physiological conditions...
  17. pmc Specific Inhibition of NEIL-initiated repair of oxidized base damage in human genome by copper and iron: potential etiological linkage to neurodegenerative diseases
    Muralidhar L Hegde
    Department of Biochemistry, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 285:28812-25. 2010
    ..Interestingly, specific chelators, including the natural chemopreventive compound curcumin, reverse the inhibition of NEILs both in vitro and in cells, suggesting their therapeutic potential...
  18. ncbi request reprint Choreography of oxidative damage repair in mammalian genomes
    Sankar Mitra
    Sealy Center for Molecular Science and Department of Human Biological Chemistry, University of Texas Medical Branch, Galveston, TX 77555, USA
    Free Radic Biol Med 33:15-28. 2002
    ....
  19. ncbi request reprint Reduced DNA double strand breaks in chlorambucil resistant cells are related to high DNA-PKcs activity and low oxidative stress
    Istvan Boldogh
    Department of Microbiology and Immunology, Sealy Center for Molecular Sciences, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA
    Toxicology 193:137-52. 2003
    ....
  20. pmc Age-dependent deficiency in import of mitochondrial DNA glycosylases required for repair of oxidatively damaged bases
    Bartosz Szczesny
    Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 100:10670-5. 2003
    ..These results indicate an age-dependent decline in the mitochondrial import of proteins needed for DNA repair and possibly for other functions...
  21. pmc Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity
    Kishor K Bhakat
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Nucleic Acids Res 32:3033-9. 2004
    ..Reversible acetylation of Lys49 could thus regulate the repair activity of NEIL2 in vivo...
  22. pmc Acetylation of human 8-oxoguanine-DNA glycosylase by p300 and its role in 8-oxoguanine repair in vivo
    Kishor K Bhakat
    Sealy Center for Molecular Science, University of Texas Medical Branch, 6 136 Medical Research Building, Route 1079, Galveston, TX 77555, USA
    Mol Cell Biol 26:1654-65. 2006
    ..Based on these results, we propose a novel regulatory function of OGG1 acetylation in repair of its substrates in oxidatively stressed cells...
  23. pmc Base-pairing properties of the oxidized cytosine derivative, 5-hydroxy uracil
    Varatharasa Thiviyanathan
    Sealy Center for Structural Biology and Molecular Biophysics, Sealy Center for Molecular Sciences, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Biochem Biophys Res Commun 366:752-7. 2008
    ..Our results explain why certain DNA polymerases preferentially incorporate G opposite to 5-OHU over A and why C does not get incorporated against 5-OHU during DNA replication in vivo...
  24. ncbi request reprint Stimulation of DNA glycosylase activity of OGG1 by NEIL1: functional collaboration between two human DNA glycosylases
    Sanath K Mokkapati
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77550, USA
    Biochemistry 43:11596-604. 2004
    ..Comparable levels of NEIL1 and OGG1 in some human cells raise the possibility that NEIL1 serves as a backup enzyme to APE1 in stimulating 8-oxoG repair in vivo...
  25. ncbi request reprint Solution structure of a DNA duplex containing 8-hydroxy-2'-deoxyguanosine opposite deoxyguanosine
    Varatharasa Thiviyanathan
    Sealy Center for Structural Biology, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1157, USA
    J Mol Biol 325:433-42. 2003
    ..The melting temperature of the 8-OG:G containing duplex is only 2.6 deg. C less than the t(m) of the unmodified duplex...
  26. pmc Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene
    Kishor K Bhakat
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    EMBO J 22:6299-309. 2003
    ..These results indicate that acetylation of APE1 plays an important role in this key repair protein's action in transcriptional regulation...
  27. ncbi request reprint Interaction of the human DNA glycosylase NEIL1 with proliferating cell nuclear antigen. The potential for replication-associated repair of oxidized bases in mammalian genomes
    Hong Dou
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    J Biol Chem 283:3130-40. 2008
    ..These results strongly support in vivo function of NEIL1 in preferential repair of oxidized bases in DNA prior to replication...
  28. pmc Mutator phenotype of mammalian cells due to deficiency of NEIL1 DNA glycosylase, an oxidized base-specific repair enzyme
    Amit K Maiti
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA
    DNA Repair (Amst) 7:1213-20. 2008
    ..NEIL1 thus plays a distinct and important role in repairing endogenous and induced mutagenic oxidized bases, and hence in maintaining the functional integrity of mammalian genomes...
  29. pmc ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation
    Istvan Boldogh
    Department of Microbiology and Immunology, University of Texas Medical Branch UTMB, Galveston, Texas 77555, USA
    J Clin Invest 115:2169-79. 2005
    ..We propose that oxidative stress generated by pollen NADPH oxidases (signal 1) augments allergic airway inflammation induced by pollen antigen (signal 2)...
  30. ncbi request reprint AP endonuclease-independent DNA base excision repair in human cells
    Lee Wiederhold
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555, USA
    Mol Cell 15:209-20. 2004
    ..That NEIL1/PNK could also repair the products of other DNA glycosylases suggests a broad role for this APE-independent BER pathway in mammals...
  31. pmc Ragweed pollen-mediated IgE-independent release of biogenic amines from mast cells via induction of mitochondrial dysfunction
    Grzegorz Chodaczek
    Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, TX 77555, USA
    Mol Immunol 46:2505-14. 2009
    ..Pharmacological maintenance of physiological mitochondrial function could have clinical benefits in prevention and treatment of allergic diseases...
  32. pmc Increased ROS generation in subsets of OGG1 knockout fibroblast cells
    Attila Bacsi
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
    Mech Ageing Dev 128:637-49. 2007
    ..Whether similar mechanisms exists in tissues of Ogg1(-/-) mice is the focus of future investigations...
  33. pmc Functions of disordered regions in mammalian early base excision repair proteins
    Muralidhar L Hegde
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1079, USA
    Cell Mol Life Sci 67:3573-87. 2010
    ..These disordered segments also include sites for posttranslational modifications and nuclear localization signal. The teleological basis for their structural flexibility is discussed...
  34. pmc Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1
    Ranajoy Chattopadhyay
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555 1079, USA
    Mol Cell Biol 28:7066-80. 2008
    ..Thus, our study has established the novel role of the acetylation-mediated transcriptional regulatory function of APE1, making it a potential target for the drug sensitization of tumor cells...
  35. pmc Identification and characterization of mitochondrial abasic (AP)-endonuclease in mammalian cells
    Ranajoy Chattopadhyay
    Sealy Center for Molecular Science, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 1079, USA
    Nucleic Acids Res 34:2067-76. 2006
    ..The low abundance of mtAPE, particularly in cultured cells might be the reason for its earlier lack of detection by western analysis...
  36. ncbi request reprint Action of human endonucleases III and VIII upon DNA-containing tandem dihydrouracil
    Mohsin M Ali
    Department of Radiation Oncology, Emory University School of Medicine, Atlanta, GA 30322, USA
    DNA Repair (Amst) 4:679-86. 2005
    ..Together, we suggest that the processing of DNA-containing tandem DHU lesions, initiated by hNTH and NEIL1 can be channeled into two sub-pathways, the PNK-independent, APE1-dependent and the PNK, APE1-dependent pathways, respectively...
  37. ncbi request reprint Enhanced gamma-glutamylcysteine synthetase activity decreases drug-induced oxidative stress levels and cytotoxicity
    Gokul C Das
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, 77555, USA
    Mol Carcinog 45:635-47. 2006
    ....
  38. pmc Oxanine DNA glycosylase activities in mammalian systems
    Liang Dong
    Department of Genetics and Biochemistry, South Carolina Experiment Station, Clemson University, Room 219, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, United States
    DNA Repair (Amst) 7:128-34. 2008
    ..ODG activity was the highest in hAAG and lowest in hSMUG1...
  39. pmc The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates NEIL1 glycosylase
    Xin Guan
    Department of Biochemistry and Molecular Biology and Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Nucleic Acids Res 35:2463-72. 2007
    ..Thus, the 9-1-1 complex at the lesion sites serves as both a damage sensor to activate checkpoint control and a component of base excision repair...

Research Grants6

  1. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2005
    ....
  2. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2006
    ....
  3. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2007
    ....
  4. Repair of oxidative damage in mammalian genomes
    TAPAS HAZRA; Fiscal Year: 2009
    ....
  5. Repair of oxidative damage in mammalian genomes
    Tapas K Hazra; Fiscal Year: 2010
    ..abstract_text> ..