Donald Guiney

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc The Role of the spv Genes in Salmonella Pathogenesis
    Donald G Guiney
    Department of Medicine, University of California San Diego School of Medicine La Jolla, CA, USA
    Front Microbiol 2:129. 2011
  2. ncbi request reprint Targeting of the actin cytoskeleton during infection by Salmonella strains
    Donald G Guiney
    Department of Medicine 0640, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    Clin Immunol 114:248-55. 2005
  3. ncbi request reprint The role of host cell death in Salmonella infections
    D G Guiney
    Department of Medicine, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA 92093 0640, USA
    Curr Top Microbiol Immunol 289:131-50. 2005
  4. pmc Helicobacter pylori preferentially induces interleukin 12 (IL-12) rather than IL-6 or IL-10 in human dendritic cells
    Donald G Guiney
    Department of Medicine, University of California, San Diego, La Jolla, California 92093 0640, USA
    Infect Immun 71:4163-6. 2003
  5. ncbi request reprint Interactions between inducible nitric oxide and other inflammatory mediators during Helicobacter pylori infection
    Marygorret Obonyo
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    Helicobacter 8:495-502. 2003
  6. ncbi request reprint Modulation of cytokine production and enhancement of cell viability by TLR7 and TLR9 ligands during anthrax infection of macrophages
    Mojgan Sabet
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 47:369-79. 2006
  7. ncbi request reprint Evidence for interleukin-1-independent stimulation of interleukin-12 and down-regulation by interleukin-10 in Helicobacter pylori-infected murine dendritic cells deficient in the interleukin-1 receptor
    Marygorret Obonyo
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 47:414-9. 2006
  8. pmc Deficiencies of myeloid differentiation factor 88, Toll-like receptor 2 (TLR2), or TLR4 produce specific defects in macrophage cytokine secretion induced by Helicobacter pylori
    Marygorret Obonyo
    School of Medicine, Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    Infect Immun 75:2408-14. 2007
  9. ncbi request reprint Vaccination with irradiated Listeria induces protective T cell immunity
    Sandip K Datta
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, 92093, USA
    Immunity 25:143-52. 2006
  10. pmc In vivo efficacy of a phosphodiester TLR-9 aptamer and its beneficial effect in a pulmonary anthrax infection model
    Christina C N Wu
    Department of Medicine, Rebecca and John Moores UCSD Cancer Center, University of California San Diego, La Jolla, CA 92093 0820, USA
    Cell Immunol 251:78-85. 2008

Research Grants

Collaborators

Detail Information

Publications24

  1. pmc The Role of the spv Genes in Salmonella Pathogenesis
    Donald G Guiney
    Department of Medicine, University of California San Diego School of Medicine La Jolla, CA, USA
    Front Microbiol 2:129. 2011
    ..This association is not seen with typhoid fever, indicating that the pathogenesis and immunology of typhoid have fundamental differences from the syndrome of non-typhoid bacteremia...
  2. ncbi request reprint Targeting of the actin cytoskeleton during infection by Salmonella strains
    Donald G Guiney
    Department of Medicine 0640, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    Clin Immunol 114:248-55. 2005
    ..SpvB produces a cytotoxic effect on infected host cells leading to apoptosis. The SpvB effect appears to promote intracellular infection and may facilitate cell-to-cell spread of the organism, thereby enhancing virulence...
  3. ncbi request reprint The role of host cell death in Salmonella infections
    D G Guiney
    Department of Medicine, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA 92093 0640, USA
    Curr Top Microbiol Immunol 289:131-50. 2005
    ..These infected, apoptotic cells are targeted for engulfment by incoming macrophages, thus perpetuating the cycle of cell-to-cell spread that is the hallmark of systemic Salmonella infection...
  4. pmc Helicobacter pylori preferentially induces interleukin 12 (IL-12) rather than IL-6 or IL-10 in human dendritic cells
    Donald G Guiney
    Department of Medicine, University of California, San Diego, La Jolla, California 92093 0640, USA
    Infect Immun 71:4163-6. 2003
    ..enterica, indicating a Th1 response. An isogenic cagE mutant of H. pylori lost about 50% of its IL-12-inducing ability, suggesting a role for the cag type IV secretion system in the stimulation of dendritic cells...
  5. ncbi request reprint Interactions between inducible nitric oxide and other inflammatory mediators during Helicobacter pylori infection
    Marygorret Obonyo
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    Helicobacter 8:495-502. 2003
    ..Inducible nitric oxide synthase has been shown to be up-regulated in the gastric epithelium during H. pylori gastritis, suggesting a role in inflammation...
  6. ncbi request reprint Modulation of cytokine production and enhancement of cell viability by TLR7 and TLR9 ligands during anthrax infection of macrophages
    Mojgan Sabet
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 47:369-79. 2006
    ..These findings suggest that TLR ligands may contribute to the enhancement of innate immunity in B. anthracis infection by suppressing potentially deleterious pro-inflammatory cytokine responses and by improving macrophage viability...
  7. ncbi request reprint Evidence for interleukin-1-independent stimulation of interleukin-12 and down-regulation by interleukin-10 in Helicobacter pylori-infected murine dendritic cells deficient in the interleukin-1 receptor
    Marygorret Obonyo
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 47:414-9. 2006
    ..pylori when the bacterial load is high, allowing for persistent colonization of the gastric mucosa...
  8. pmc Deficiencies of myeloid differentiation factor 88, Toll-like receptor 2 (TLR2), or TLR4 produce specific defects in macrophage cytokine secretion induced by Helicobacter pylori
    Marygorret Obonyo
    School of Medicine, Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    Infect Immun 75:2408-14. 2007
    ..pylori-related gastric cancer. In conclusion, our study demonstrates that H. pylori activates TLR2 and TLR4, leading to the secretion of distinct cytokines by macrophages...
  9. ncbi request reprint Vaccination with irradiated Listeria induces protective T cell immunity
    Sandip K Datta
    Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, 92093, USA
    Immunity 25:143-52. 2006
    ..gamma-irradiation may be potentially applied to numerous bacterial vaccine candidates, and irradiated bacteria could serve as a vaccine platform for recombinant antigens derived from other pathogens, allergens, or tumors...
  10. pmc In vivo efficacy of a phosphodiester TLR-9 aptamer and its beneficial effect in a pulmonary anthrax infection model
    Christina C N Wu
    Department of Medicine, Rebecca and John Moores UCSD Cancer Center, University of California San Diego, La Jolla, CA 92093 0820, USA
    Cell Immunol 251:78-85. 2008
    ..Hence, the transient effects from a PO TLR-9 agonist may be beneficial for protection in a bacterial bioterrorism attack, by delaying the onset of systemic infection without the induction of a cytokine syndrome...
  11. pmc Characterization of Salmonella-induced cell death in human macrophage-like THP-1 cells
    Eulalia Valle
    Division of Infectious Diseases, Department of Medicine, UCSD School of Medicine, 9500 Gilman Dr, La Jolla, CA 92093 0640, USA
    Infect Immun 73:2835-40. 2005
    ..However, inhibition of caspase-3 did not block Salmonella-induced DNA fragmentation. These results identify a previously undetected apoptotic effect in Salmonella-infected cells that is dependent on phoP gene function...
  12. doi request reprint Identification of Salmonella SPI-2 secretion system components required for SpvB-mediated cytotoxicity in macrophages and virulence in mice
    Sara H Browne
    Department of Medicine 0640, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 52:194-201. 2008
    ..In contrast, competitive infection studies in mice confirmed that SpvB and SifA have independent virulence effects, as predicted by the macrophage studies...
  13. ncbi request reprint The protein kinase PKR is required for macrophage apoptosis after activation of Toll-like receptor 4
    Li Chung Hsu
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0636, USA
    Nature 428:341-5. 2004
    ..The pro-apoptotic actions of PKR are mediated both through inhibition of protein synthesis and activation of interferon response factor 3...
  14. pmc Genetic requirements for salmonella-induced cytopathology in human monocyte-derived macrophages
    Sara H Browne
    Department of Medicine, School of Medicine, University of California at San Diego, La Jolla 92093 0640, USA
    Infect Immun 70:7126-35. 2002
    ..We conclude that SpvB and a functional SPI2 TTSS are essential for Salmonella-induced delayed cytotoxicity of human macrophages...
  15. pmc Role of gamma interferon in Helicobacter pylori induction of inflammatory mediators during murine infection
    Marygorret Obonyo
    Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Infect Immun 70:3295-9. 2002
    ..pylori in GSM06 cells. Induction of cyclooxygenase 2 mRNA through IFN-gamma was demonstrated in GSM06 cells. These data indicate that IFN-gamma mediates the induction of MIP-2 and iNOS mRNA expression by H. pylori in mice...
  16. pmc ClpX contributes to innate defense peptide resistance and virulence phenotypes of Bacillus anthracis
    Shauna M McGillivray
    Department of Pediatrics, University of California San Diego, La Jolla, CA 92093 0687, USA
    J Innate Immun 1:494-506. 2009
    ..We conclude that ClpX is an important factor allowing B. anthracis to subvert host immune clearance mechanisms, and thus represents a novel therapeutic target for prevention or therapy of anthrax, a foremost biodefense concern...
  17. ncbi request reprint Genes in the Salmonella pathogenicity island 2 and the Salmonella virulence plasmid are essential for Salmonella-induced apoptosis in intestinal epithelial cells
    Günther Paesold
    Department of Medicine 0623D, University of California at San Diego, La Jolla, CA 92093 0623, USA
    Cell Microbiol 4:771-81. 2002
    ..In addition to their influence on intracellular proliferation of Salmonella, genes in those loci determine the ultimate fate of infected epithelial cells with respect to caspase 3 activation and undergoing death by apoptosis...
  18. ncbi request reprint CpG oligonucleotides partially inhibit growth of Mycobacterium tuberculosis, but not Salmonella or Listeria, in human monocyte-derived macrophages
    Jennifer P Wang
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    FEMS Immunol Med Microbiol 45:303-10. 2005
    ..These data indicate that CpG-ODN can improve the ability of hMDM to contain growth of virulent M. tuberculosis...
  19. doi request reprint Improving reverse vaccinology with a machine learning approach
    Brett N Bowman
    Bioinformatics and Medical Informatics, San Diego State University, San Diego, CA 92182, USA
    Vaccine 29:8156-64. 2011
    ..05). This paper describes a superior workflow for performing reverse vaccinology studies and provides a benchmark training data set that can be used to evaluate future methodological improvements...
  20. ncbi request reprint Targeting host cell furin proprotein convertases as a therapeutic strategy against bacterial toxins and viral pathogens
    Sergey A Shiryaev
    Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Biol Chem 282:20847-53. 2007
    ..Our results confirm that inhibiting furin-like PCs protects the host from the distinct furin-dependent infections and lay a foundation for novel, host cell-focused therapies against acute diseases...
  21. pmc Characterization of monospecies biofilm formation by Helicobacter pylori
    Sheri P Cole
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0640, USA
    J Bacteriol 186:3124-32. 2004
    ..These results indicate the relative importance of these genes to the production of biofilms by H. pylori and the selective enhancement of planktonic growth in the presence of gastric mucin...
  22. ncbi request reprint Gene expression profiling detects patterns of human macrophage responses following Mycobacterium tuberculosis infection
    Jennifer P Wang
    University of California, San Diego, Department of Medicine 0640, 9500 Gilman Drive, La Jolla, CA 92093 0640, USA
    FEMS Immunol Med Microbiol 39:163-72. 2003
    ..Augmentation of the respiratory burst and preservation of the response to interferon-gamma were also demonstrated. These data supplement existing knowledge on macrophage responses to tuberculosis infection...
  23. ncbi request reprint Salmonella secreted factor L deubiquitinase of Salmonella typhimurium inhibits NF-kappaB, suppresses IkappaBalpha ubiquitination and modulates innate immune responses
    Gaelle Le Negrate
    Burnham Institute for Medical Research, La Jolla, CA 92037
    J Immunol 180:5045-56. 2008
    ..Thus, SseL represents one of the first bacterial deubiquitinases demonstrated to modulate the host inflammatory response in vivo...
  24. pmc Characterization of the spv locus in Salmonella enterica serovar Arizona
    Stephen J Libby
    Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695 7615, USA
    Infect Immun 70:3290-4. 2002
    ..enterica, with plasmid acquisition of spv genes in the subspecies I strains involving addition of spvD and polymorphisms in spvA...

Research Grants3

  1. INTERACTIONS OF HELICOBACTER WITH THE GASTRIC MUCOSA
    Donald Guiney; Fiscal Year: 2001
    ..Finally, iNOS, IL-4, IL-10 and IFN-g knockout mice will also be used to determine the importance of these enzymes and cytokines in H. pylon-induced gastritis. ..
  2. Salmonella-to-Eukaryotic Cell Multi-task Gene Delivery
    Donald Guiney; Fiscal Year: 2006
    ..These studies are intended to develop attenuated Salmonella strains for DNA delivery for DNA vaccine approaches, corrective gene therapy, immunotherapy, and the prevention and treatment of certain kinds of cancer. ..
  3. PLASMID-MEDIATED VIRULENCE IN SALMONELLA
    Donald Guiney; Fiscal Year: 2008
    ..unreadable] [unreadable]..