Mark Goulian

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Robust control in bacterial regulatory circuits
    Mark Goulian
    Department of Physics and Institute for Medicine and Engineering, University of Pennsylvania, 209 S 33rd Street, Philadelphia, PA 19104, USA
    Curr Opin Microbiol 7:198-202. 2004
  2. pmc Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli
    Albert Siryaporn
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Microbiol 70:494-506. 2008
  3. pmc Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli
    Andrew M Lippa
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Bacteriol 194:1457-63. 2012
  4. pmc Evolving a robust signal transduction pathway from weak cross-talk
    Albert Siryaporn
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Mol Syst Biol 6:452. 2010
  5. ncbi request reprint Imaging OmpR localization in Escherichia coli
    Eric Batchelor
    Department of Physics, University of Pennsylvania, Philadelphia, 19104, USA
    Mol Microbiol 59:1767-78. 2006
  6. pmc High stimulus unmasks positive feedback in an autoregulated bacterial signaling circuit
    Tim Miyashiro
    Departments of Biology and Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:17457-62. 2008
  7. pmc The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC
    Eric Batchelor
    Department of Physics, University of Pennsylvania, 209 S 33rd St, Philadelphia, PA 19104, USA
    J Bacteriol 187:5723-31. 2005
  8. pmc Escherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts
    Melissa Lasaro
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Bacteriol 196:1723-32. 2014
  9. pmc Imaging OmpR binding to native chromosomal loci in Escherichia coli
    Elizabeth A Libby
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6018, USA
    J Bacteriol 192:4045-53. 2010
  10. pmc Feedback inhibition in the PhoQ/PhoP signaling system by a membrane peptide
    Andrew M Lippa
    Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    PLoS Genet 5:e1000788. 2009

Collaborators

  • Jun Zhu
  • Tim Miyashiro
  • Hui Wang
  • Zhigang Zhou
  • Massimiliano Bonomi
  • Andrej Sali
  • Biao Kan
  • Sujay Chattopadhyay
  • Jing Zhang
  • Lin Guo
  • LINDA KENNEY
  • Michael T Laub
  • Manuela Roggiani
  • Albert Siryaporn
  • Eric Batchelor
  • Zhi Liu
  • Andrew M Lippa
  • Shalom D Goldberg
  • Elizabeth A Libby
  • William F Degrado
  • Srujana S Yadavalli
  • Charlie Y Mo
  • Paige Derr
  • Kathleen S Molnar
  • Melissa Lasaro
  • Sri Ram
  • David Chow
  • Graham D Clinthorne
  • Melissa A Lasaro
  • Barrett S Perchuk
  • Jeffrey M Skerker
  • Fu Xiang
  • Andrew M Stern
  • Rahul M Kohli
  • Matthew J Culyba
  • Paul D Sniegowski
  • Nawar Naseer
  • Amanda N Samuels
  • Sara A Manning
  • Rachel S Leibman
  • Jeffrey N Carey
  • Annie I Chen
  • Riccardo Pellarin
  • Evgeni Sokurenko
  • Sandip Paul
  • Rima Bishar
  • KATHRYN KELLY
  • Gabriel Gonzalez
  • Feng Gai
  • Seda Ekici
  • Yantao Wang
  • Nina Salinger
  • Zhengtao Zhong
  • Emma A Lubin
  • Orr Ashenberg
  • Ansel Hsiao
  • Amy Tsou
  • Eric Boder
  • Don Walthers
  • Thomas J Silhavy

Detail Information

Publications32

  1. ncbi request reprint Robust control in bacterial regulatory circuits
    Mark Goulian
    Department of Physics and Institute for Medicine and Engineering, University of Pennsylvania, 209 S 33rd Street, Philadelphia, PA 19104, USA
    Curr Opin Microbiol 7:198-202. 2004
    ..Various forms of robustness are associated with circuits based on negative and/or positive feedback, bi-functional enzymes, protein oligomerization and discrete or continuous control...
  2. pmc Cross-talk suppression between the CpxA-CpxR and EnvZ-OmpR two-component systems in E. coli
    Albert Siryaporn
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Microbiol 70:494-506. 2008
    ..e. it masks the effects of mutations that would otherwise lead to increased cross-talk. The mechanisms that provide insulation against interference described here may be applicable to many other two-component systems...
  3. pmc Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli
    Andrew M Lippa
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Bacteriol 194:1457-63. 2012
    ..Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system...
  4. pmc Evolving a robust signal transduction pathway from weak cross-talk
    Albert Siryaporn
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Mol Syst Biol 6:452. 2010
    ....
  5. ncbi request reprint Imaging OmpR localization in Escherichia coli
    Eric Batchelor
    Department of Physics, University of Pennsylvania, Philadelphia, 19104, USA
    Mol Microbiol 59:1767-78. 2006
    ..Our results suggest that, at least under our growth conditions, procaine activates EnvZ-OmpR signalling whereas osmolarity has, at best, a weak effect on the EnvZ-OmpR system...
  6. pmc High stimulus unmasks positive feedback in an autoregulated bacterial signaling circuit
    Tim Miyashiro
    Departments of Biology and Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:17457-62. 2008
    ..Our results demonstrate a simple and general mechanism for regulating the positive feedback associated with autoregulation within a bacterial signaling circuit to boost response range and maintain a relatively uniform and graded output...
  7. pmc The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC
    Eric Batchelor
    Department of Physics, University of Pennsylvania, 209 S 33rd St, Philadelphia, PA 19104, USA
    J Bacteriol 187:5723-31. 2005
    ..However, at least for the classical porins OmpF and OmpC, our results show that the Cpx envelope stress response system plays a role in regulating their expression...
  8. pmc Escherichia coli isolate for studying colonization of the mouse intestine and its application to two-component signaling knockouts
    Melissa Lasaro
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Bacteriol 196:1723-32. 2014
    ....
  9. pmc Imaging OmpR binding to native chromosomal loci in Escherichia coli
    Elizabeth A Libby
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6018, USA
    J Bacteriol 192:4045-53. 2010
    ....
  10. pmc Feedback inhibition in the PhoQ/PhoP signaling system by a membrane peptide
    Andrew M Lippa
    Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    PLoS Genet 5:e1000788. 2009
    ..This new regulator may function as a point of control that integrates additional input signals to modulate the activity of this important signaling system...
  11. pmc Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ
    Shalom D Goldberg
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 107:8141-6. 2010
    ....
  12. pmc Stimulus-dependent differential regulation in the Escherichia coli PhoQ PhoP system
    Tim Miyashiro
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 104:16305-10. 2007
    ....
  13. pmc Fluorescence correlation spectroscopy measurements of the membrane protein TetA in Escherichia coli suggest rapid diffusion at short length scales
    David Chow
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 7:e48600. 2012
    ..These results suggest there is a population of TetA-YFP molecules in the membrane that move rapidly over short length scales (~ 400 nm) but move significantly more slowly over the longer length scales probed by FRAP...
  14. pmc Membrane protein expression triggers chromosomal locus repositioning in bacteria
    Elizabeth A Libby
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 109:7445-50. 2012
    ..Given that membrane protein-encoding genes are distributed throughout the chromosome, their expression may be an important mechanism for maintaining the bacterial chromosome in an expanded and dynamic state...
  15. ncbi request reprint Single-cell analysis of gene expression by fluorescence microscopy
    Tim Miyashiro
    Department of Physics, University of Pennsylvania, Philadelphia, PA, USA
    Methods Enzymol 423:458-75. 2007
    ..This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells...
  16. pmc Engineered single- and multi-cell chemotaxis pathways in E. coli
    Shalom D Goldberg
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6018, USA
    Mol Syst Biol 5:283. 2009
    ..The result effectively introduces a logical 'AND' into the system so that the population only swims towards the combined gradients of two attractants...
  17. pmc The architecture of a prototypical bacterial signaling circuit enables a single point mutation to confer novel network properties
    Sri Ram
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS Genet 9:e1003706. 2013
    ..Our results demonstrate the remarkable versatility of the prototypical two-component signaling architecture and highlight the tradeoffs in the particular case of the PhoQ/PhoP system. ..
  18. pmc F1C fimbriae play an important role in biofilm formation and intestinal colonization by the Escherichia coli commensal strain Nissle 1917
    Melissa A Lasaro
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Appl Environ Microbiol 75:246-51. 2009
    ..Further study of the requirements for robust biofilm formation may improve the therapeutic efficacy of Nissle 1917...
  19. pmc Mucosal penetration primes Vibrio cholerae for host colonization by repressing quorum sensing
    Zhi Liu
    Departments of Microbiology, Physics, and Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:9769-74. 2008
    ..Our results suggest that V. cholerae uses flagellar machinery to sense particular intestinal signals before colonization and enhance the expression of virulence genes by modulating the output of quorum sensing signaling...
  20. ncbi request reprint Changing the specificity of a bacterial chemoreceptor
    Paige Derr
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 355:923-32. 2006
    ....
  21. pmc Cys-scanning disulfide crosslinking and bayesian modeling probe the transmembrane signaling mechanism of the histidine kinase, PhoQ
    Kathleen S Molnar
    Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA Biochemistry and Molecular Biophysics Graduate Group19104, USA, University of Pennsylvania, Philadelphia, PA 19104, USA Department of Pharmaceutical Chemistry, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA
    Structure 22:1239-51. 2014
    ..Because a similar scissors model has been ruled out in CheA-linked chemoreceptors, the evidence suggests that sensor His-kinase and CheA-linked receptors possess different signaling mechanisms. ..
  22. doi request reprint Characterizing cross-talk in vivo avoiding pitfalls and overinterpretation
    Albert Siryaporn
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Methods Enzymol 471:1-16. 2010
    ..We address potential complications associated with measuring cross-talk and discuss some methods for identifying and unmasking sources of cross-talk in cells using transcriptional reporters and in vivo DNA-binding assays...
  23. ncbi request reprint A simple system for converting lacZ to gfp reporter fusions in diverse bacteria
    Mark Goulian
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Gene 372:219-26. 2006
    ....
  24. pmc Two-component signaling circuit structure and properties
    Mark Goulian
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Curr Opin Microbiol 13:184-9. 2010
    ..Examples where recent progress has been made include aspects of autoregulation, signal integration in branched pathways, cross-talk suppression, and cross-regulation via connector proteins...
  25. pmc Antimicrobial peptides trigger a division block in Escherichia coli through stimulation of a signalling system
    Srujana S Yadavalli
    Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Commun 7:12340. 2016
    ..Regulation of septation by PhoQ/PhoP may protect cells from antimicrobial peptide-induced stress or other conditions associated with high-level stimulation of this signalling system. ..
  26. pmc Differential Thiol-Based Switches Jump-Start Vibrio cholerae Pathogenesis
    Zhi Liu
    Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell Rep 14:347-54. 2016
    ..This reveals a mechanism whereby bacterial pathogens employ posttranslational modifications of multiple transcription factors to sense and adapt to dynamic environmental changes. ..
  27. pmc Robustness and the cycle of phosphorylation and dephosphorylation in a two-component regulatory system
    Eric Batchelor
    Department of Physics and Astronomy, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 100:691-6. 2003
    ..Using these strains we have shown that, consistent with the predictions of our model, the transcription of ompC and ompF is indeed robust or insensitive to a wide range of expression levels of both EnvZ and OmpR...
  28. pmc Systematically Altering Bacterial SOS Activity under Stress Reveals Therapeutic Strategies for Potentiating Antibiotics
    Charlie Y Mo
    Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA
    mSphere 1:. 2016
    ....
  29. doi request reprint Chromosome-Membrane Interactions in Bacteria
    Manuela Roggiani
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 email
    Annu Rev Genet 49:115-29. 2015
    ..This review summarizes the history and recent results of chromosome-membrane associations and discusses the known and theorized consequences of these interactions in the bacterial cell. ..
  30. pmc Oxygen-Dependent Cell-to-Cell Variability in the Output of the Escherichia coli Tor Phosphorelay
    Manuela Roggiani
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Bacteriol 197:1976-87. 2015
    ..Thus, oxygen regulates the variance of the output but not the mean for the Tor system. We further show that this oxygen-dependent variability stems from the phosphorelay...
  31. pmc Continuous control in bacterial regulatory circuits
    Eric Batchelor
    Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Bacteriol 186:7618-25. 2004
    ..This mode of control is particularly interesting for an organism such as E. coli, which proliferates in diverse environments, and raises important questions regarding the biologically relevant inputs and outputs for this system...
  32. pmc Rewiring the specificity of two-component signal transduction systems
    Jeffrey M Skerker
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 133:1043-54. 2008
    ....