Rafael Garcia-Mata

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc The 'invisible hand': regulation of RHO GTPases by RHOGDIs
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
    Nat Rev Mol Cell Biol 12:493-504. 2011
  2. ncbi Catching a GEF by its tail
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 12 026 Lineberger, CB 7295, Chapel Hill, NC 27599, USA
    Trends Cell Biol 17:36-43. 2007
  3. pmc The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 12 026 Lineberger, CB 7295, Chapel Hill, NC 27599, USA
    Mol Cell Biol 27:8683-97. 2007
  4. pmc Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus
    Christophe Guilluy
    Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 16:376-81. 2014
  5. pmc The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals
    Adi D Dubash
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e17380. 2011
  6. pmc RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration
    Jaap D van Buul
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 178:1279-93. 2007
  7. ncbi Analysis of activated GAPs and GEFs in cell lysates
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA
    Methods Enzymol 406:425-37. 2006
  8. doi Chapter 1. Focal adhesions: new angles on an old structure
    Adi D Dubash
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, CB 7295, University of North Carolina, Chapel Hill, NC 27599, USA
    Int Rev Cell Mol Biol 277:1-65. 2009
  9. pmc Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
    Etienne Boulter
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 12:477-83. 2010
  10. ncbi A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin
    Adi D Dubash
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill NC 27599, USA
    J Cell Sci 120:3989-98. 2007

Collaborators

Detail Information

Publications18

  1. pmc The 'invisible hand': regulation of RHO GTPases by RHOGDIs
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
    Nat Rev Mol Cell Biol 12:493-504. 2011
    ..The same metaphor can be used to describe the RHO-specific guanine nucleotide dissociation inhibitor (RHOGDI) family, which operates in the background, as an invisible hand, using similar forces to regulate the RHO GTPase cycle...
  2. ncbi Catching a GEF by its tail
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 12 026 Lineberger, CB 7295, Chapel Hill, NC 27599, USA
    Trends Cell Biol 17:36-43. 2007
    ..Here, we summarize recent data that highlight the importance of these interactions in Rho-GEF regulation...
  3. pmc The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 12 026 Lineberger, CB 7295, Chapel Hill, NC 27599, USA
    Mol Cell Biol 27:8683-97. 2007
    ..Together, our results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation...
  4. pmc Isolated nuclei adapt to force and reveal a mechanotransduction pathway in the nucleus
    Christophe Guilluy
    Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 16:376-81. 2014
    ..Our results demonstrate that mechanotransduction is not restricted to cell surface receptors and adhesions but can occur in the nucleus. ..
  5. pmc The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals
    Adi D Dubash
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e17380. 2011
    ....
  6. pmc RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration
    Jaap D van Buul
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 178:1279-93. 2007
    ..Together, these results identify a new signaling pathway involving RhoG and its exchange factor SGEF downstream from ICAM1 that is critical for leukocyte TEM...
  7. ncbi Analysis of activated GAPs and GEFs in cell lysates
    Rafael Garcia-Mata
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA
    Methods Enzymol 406:425-37. 2006
    ..This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals...
  8. doi Chapter 1. Focal adhesions: new angles on an old structure
    Adi D Dubash
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, CB 7295, University of North Carolina, Chapel Hill, NC 27599, USA
    Int Rev Cell Mol Biol 277:1-65. 2009
    ..Throughout this chapter, we have highlighted areas of focal adhesion biology where major questions still remain to be answered...
  9. pmc Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1
    Etienne Boulter
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 12:477-83. 2010
    ....
  10. ncbi A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin
    Adi D Dubash
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill NC 27599, USA
    J Cell Sci 120:3989-98. 2007
    ..These data establish a previously uncharacterized role for the exchange factors Lsc/p115 RhoGEF and LARG in linking fibronectin signals to downstream RhoA activation...
  11. pmc Rho protein crosstalk: another social network?
    Christophe Guilluy
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Trends Cell Biol 21:718-26. 2011
    ..In the second part we illustrate the importance of Rho protein crosstalk with two examples: integrin-based adhesion and cell migration...
  12. ncbi Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells
    Silvia Goicoechea
    Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, NC, USA
    J Cell Sci 119:3316-24. 2006
    ....
  13. doi Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus
    Christophe Guilluy
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, North Carolina, USA
    Nat Protoc 6:2050-60. 2011
    ..Completion of the procedure, including nuclear isolation and RhoA or Rho GEF activity assay, takes 1 h 40 min. We also include details of how to perform a basic assay of whole-cell extracts...
  14. pmc Plexin-B2 negatively regulates macrophage motility, Rac, and Cdc42 activation
    Kelly E Roney
    Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e24795. 2011
    ..The data presented here show that Plexin-B2 functions in mouse macrophages as a negative regulator of the GTPases Rac and Cdc42 and as a negative regulator of basal cell motility and wound healing...
  15. pmc The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins
    Christophe Guilluy
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Nat Cell Biol 13:722-7. 2011
    ..Our results reveal that LARG is activated by the Src family tyrosine kinase Fyn, whereas GEF-H1 catalytic activity is enhanced by ERK downstream of a signalling cascade that includes FAK and Ras...
  16. pmc The UDP-sugar-sensing P2Y(14) receptor promotes Rho-mediated signaling and chemotaxis in human neutrophils
    Juliana I Sesma
    Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7248, USA
    Am J Physiol Cell Physiol 303:C490-8. 2012
    ..These results support the notion that UDP-glucose is a stable and potent proinflammatory mediator that promotes P2Y(14)-R-mediated neutrophil motility via Rho/Rho kinase activation...
  17. doi Simvastatin inhibits secretion of Th17-polarizing cytokines and antigen presentation by DCs in patients with relapsing remitting multiple sclerosis
    Xin Zhang
    Department of Neurology, University of North Carolina at Chapel Hill, NC, USA
    Eur J Immunol 43:281-9. 2013
    ....
  18. pmc Roles of the small GTPases RhoA and Rac1 in cell behavior
    Carol Otey
    Department of Cellular and Molecular Physiology, University of North Carolina at Chapel Hill, 5320 MBRB, Chapel Hill, NC, USA
    F1000 Biol Rep 1:4. 2009
    ..Recent work indicates their involvement in signaling between the adhesion receptors integrin and syndecan, effects on the recruitment of beta-catenin to the nucleus, and potential roles in the nucleus as well as the cytoplasm...