Genomes and Genes
R B Doctor
Affiliation: University of Colorado Health Sciences Center
- Cholangiocytes exhibit dynamic, actin-dependent apical membrane turnoverR Brian Doctor
Division of Gastroenterology Hepatology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Am J Physiol Cell Physiol 282:C1042-52. 2002..These studies indicate that there is a high rate of vesicular trafficking at the apical membrane of cholangiocytes and suggest that both cAMP and the actin cytoskeleton contribute importantly to these events...
- Emerging roles of the actin cytoskeleton in cholangiocyte function and diseaseR Brian Doctor
Division of Gastroenterology and Hepatology, University of Colorado Health Sciences Center, Denver, Colorado, USA
Semin Liver Dis 22:263-76. 2002..Although much remains to be discovered, this article will highlight observations that indicate that the actin cytoskeleton plays a central role in the physiology and diseases of the intrahepatic bile duct...
- Reorganization of cholangiocyte membrane domains represents an early event in rat liver ischemiaR B Doctor
University of Colorado Health Sciences Center, Division of Gastroenterology and Hepatology, Denver, CO 80262, USA
Hepatology 29:1364-74. 1999..Pending the re-establishment of the differentiated domains, the loss of specific secondary structures may contribute to impaired vectorial bile duct secretion and postischemic cholestasis...
- Regulated ion transport in mouse liver cyst epithelial cellsR Brian Doctor
Division of Gastroenterology, University of Colorado Health Sciences Center, Box B158, 4200 E 9th Avenue, Denver, CO 80262, USA
Biochim Biophys Acta 1772:345-54. 2007..In conclusion, regulated ion transport pathways are present in liver cyst epithelia and are positioned to direct fluid secretion into the lumen of liver cysts and promote increases in liver cyst expansion and growth...
- The lipid products of phosphoinositide 3-kinase contribute to regulation of cholangiocyte ATP and chloride transportA P Feranchak
Department of Pediatrics, Children s Hospital, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Biol Chem 274:30979-86. 1999..Taken together, these findings indicate that volume-sensitive activation of PI 3-kinase and the generation of lipid messengers modulate cholangiocyte ATP release, Cl(-) secretion, and, hence, bile formation...
- ATP depletion in rat cholangiocytes leads to marked internalization of membrane proteinsR B Doctor
University of Colorado Health Sciences Center, Denver, CO 80262, USA
Hepatology 31:1045-54. 2000..This sustained decrease in transport capacity may contribute to the development of ductular injury in postischemic livers...
- Ezrin-radixin-moesin-binding phosphoprotein 50 is expressed at the apical membrane of rat liver epitheliaL Fouassier
Division of Gastroenterology and Hepatology, University of Colorado Health Sciences Center, Denver, 80262, USA
Hepatology 33:166-76. 2001....
- ClC-2 chloride channels contribute to HTC cell volume homeostasisR M Roman
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
Am J Physiol Gastrointest Liver Physiol 280:G344-53. 2001..These studies provide further evidence that mammalian ClC-2 channel proteins are functional and suggest that in HTC cells they contribute to physiological changes in membrane Cl(-) permeability and cell volume homeostasis...
- Insulin stimulates membrane conductance in a liver cell line: evidence for insertion of ion channels through a phosphoinositide 3-kinase-dependent mechanismG Kilic
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Biol Chem 276:26762-8. 2001..Thus, regulated insertion of a separate population of ion channel-containing vesicles may represent one mechanism for mediating the changes in membrane conductance that are essential for the cellular response to insulin...
- Evidence for ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) self-association through PDZ-PDZ interactionsL Fouassier
Division of Gastroenterology and Hepatology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
J Biol Chem 275:25039-45. 2000..The ability to self-associate provides a mechanism for EBP50 to expand its capacity to form multiprotein complexes and regulate membrane transport events...
- CXCR2 agonists in ADPKD liver cyst fluids promote cell proliferationClaudia R Amura
Division of Gastroenterology, Department of Medicine, University of Colorado Health Sciences Center, Box B 146, Denver, CO 80262, USA
Am J Physiol Cell Physiol 294:C786-96. 2008..An upstream element between -272 and -1,481 bp allowed for the stress-induced increase in IL-8 transcription. These studies support the hypothesis that CXCR2 signaling promotes ADPKD liver cyst growth...
- Characterization of ionotrophic purinergic receptors in hepatocytesDaniel S Emmett
University of Texas Southwestern Medical Center, Dallas, TX 75390 9030, USA
Hepatology 47:698-705. 2008..3-3 microM) each increased intracellular [Ca(2+)]. Exposure to BzATP decreased cellular glycogen content; and P2X4 receptor messenger RNA increased in glycogen-rich liver samples...
- VEGF receptor inhibition blocks liver cyst growth in pkd2(WS25/-) miceClaudia R Amura
Department of Medicine, University of Colorado Health Sciences Center, Denver, USA
Am J Physiol Cell Physiol 293:C419-28. 2007....
- Purinergic regulation of cholangiocyte secretion: identification of a novel role for P2X receptorsR Brian Doctor
UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Am J Physiol Gastrointest Liver Physiol 288:G779-86. 2005..Functional studies indicate that P2X4 is likely the primary isoform involved, representing a novel and functionally important component of the purinergic signaling complex modulating biliary secretion...
- Polycystic disease of the liverGregory T Everson
Division of Gastroenterology and Hepatology, University of Colorado School of Medicine, Denver, CO 80262, USA
Hepatology 40:774-82. 2004..There is no effective medical therapy. Interventional and surgical options include cyst aspiration and sclerosis, open or laparoscopic cyst fenestration, hepatic resection, and liver transplantation...
- Calcium-dependent regulation of secretion in biliary epithelial cells: the role of apamin-sensitive SK channelsAndrew P Feranchak
University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, Colorado 80262, USA
Gastroenterology 127:903-13. 2004..We have identified a Ca 2+ -activated K + channel (SK2) in biliary epithelium that is inhibited by apamin. The purpose of the present studies was to define the role of SK channels in Ca 2+ -dependent cholangiocyte secretion...
- Characterization of an ankyrin repeat-containing Shank2 isoform (Shank2E) in liver epithelial cellsRyan R McWilliams
Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80439, USA
Biochem J 380:181-91. 2004..These findings indicate epithelial cells express an ankyrin repeat-containing Shank2 isoform, termed Shank2E, that is poised to co-ordinate actin-dependent events at the apical membrane...
- Increased susceptibility of fat-laden Zucker-rat hepatocytes to bile acid-induced oncotic necrosis: an in vitro model of steatocholestasisGregory E Kobak
Pediatric Liver Center and Liver Transplantation Program, Section of Pediatric Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, University of Colorado School of Medicine, Denver, USA
J Lab Clin Med 145:247-62. 2005..The protective effects of antioxidants and MPT blockers suggest novel therapeutic strategies for the treatment of steatocholestatic metabolic liver diseases...
- Shank2E binds NaP(i) cotransporter at the apical membrane of proximal tubule cellsRyan R McWilliams
Department of Medicine, University of Colorado Health Sciences Center, 4200 E 9th St, Denver, Colorado 80262, USA
Am J Physiol Cell Physiol 289:C1042-51. 2005..In sum, Shank2E is concentrated in the apical domain of renal PT cells, specifically binds NaP(i)-IIa via PDZ interactions, and undergoes P(i)-induced internalization...
- Shank2 associates with and regulates Na+/H+ exchanger 3Wonsun Han
Department of Pharmacology, Brain Korea 21 Project for Medical Science, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul 120 752, Korea
J Biol Chem 281:1461-9. 2006..These results indicate that Shank2 is a novel NHE3 interacting protein that is involved in the fine regulation of transepithelial salt and water transport through affecting NHE3 expression and activity...
- Bicarbonate-rich choleresis induced by secretin in normal rat is taurocholate-dependent and involves AE2 anion exchangerJesus M Banales
Laboratory of Molecular Genetics, Division of Gene Therapy and Hepatology, University of Navarra School of Medicine, Clinica Universitaria and CIMA, Pamplona, Spain
Hepatology 43:266-75. 2006..In conclusion, maintenance of the bile acid pool is crucial for secretin to induce bicarbonate-rich choleresis in the normal rat and that this occurs via a chloride-bicarbonate exchange process consistent with AE2 function...
- Secretion of cytokines and growth factors into autosomal dominant polycystic kidney disease liver cyst fluidMatthew T Nichols
Division of Gastroenterology, University of Colorado Health Sciences Center, Denver, CO, USA
Hepatology 40:836-46. 2004..Interruption of these signaling pathways may provide therapeutic targets for inhibiting liver cyst expansion...
- Polycystic liver: clinical characteristics of patients with isolated polycystic liver disease compared with patients with polycystic liver and autosomal dominant polycystic kidney diseaseInge A Hoevenaren
Department of Medicine, Division of Gastroenterology and Hepatology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
Liver Int 28:264-70. 2008..The goal of this study was to compare the clinical features of patients with isolated polycystic liver disease (PCLD) with those of patients with polycystic liver and autosomal dominant polycystic kidney disease (ADPKD)...