Arshad Desai

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Differential role of CENP-A in the segregation of holocentric C. elegans chromosomes during meiosis and mitosis
    Joost Monen
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, UCSD Biomedical Sciences Graduate Program, UCSD CMM E, Room 3052, 9500 Gilman Drive, La Jolla, CA 92093 0653, USA
    Nat Cell Biol 7:1248-55. 2005
  2. pmc The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
    Susan L Kline
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA
    J Cell Biol 173:9-17. 2006
  3. pmc Orientation and structure of the Ndc80 complex on the microtubule lattice
    Elizabeth M Wilson-Kubalek
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 182:1055-61. 2008
  4. pmc Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin
    Paul S Maddox
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 176:757-63. 2007
  5. pmc Tension sensing by Aurora B kinase is independent of survivin-based centromere localization
    Christopher S Campbell
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92037, USA
    Nature 497:118-21. 2013
  6. pmc Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore
    Julien Espeut
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92037, USA
    J Cell Biol 196:469-82. 2012
  7. ncbi request reprint The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East, Room 3052, La Jolla, CA 92093, USA
    Cell 127:983-97. 2006
  8. pmc A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
    Genes Dev 18:2255-68. 2004
  9. pmc A kinetochore-independent mechanism drives anaphase chromosome separation during acentrosomal meiosis
    Julien Dumont
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093 0653, USA
    Nat Cell Biol 12:894-901. 2010
  10. pmc Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly
    Molly M Lettman
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Dev Cell 25:284-98. 2013

Research Grants

  1. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2010
  2. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2005
  3. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2006
  4. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2007
  5. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2009
  6. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2010

Detail Information

Publications73

  1. ncbi request reprint Differential role of CENP-A in the segregation of holocentric C. elegans chromosomes during meiosis and mitosis
    Joost Monen
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, UCSD Biomedical Sciences Graduate Program, UCSD CMM E, Room 3052, 9500 Gilman Drive, La Jolla, CA 92093 0653, USA
    Nat Cell Biol 7:1248-55. 2005
    ..These findings highlight a pronounced difference between the segregation of holocentric chromosomes during meiosis and mitosis and demonstrate the potential to uncouple assembly of outer-kinetochore proteins from CENP-A chromatin...
  2. pmc The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
    Susan L Kline
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA
    J Cell Biol 173:9-17. 2006
    ..These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly...
  3. pmc Orientation and structure of the Ndc80 complex on the microtubule lattice
    Elizabeth M Wilson-Kubalek
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 182:1055-61. 2008
    ....
  4. pmc Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin
    Paul S Maddox
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 176:757-63. 2007
    ..These results implicate a new protein class in the assembly of centromeric chromatin and suggest that holocentric and monocentric chromosomes share a common mechanism for CENP-A loading...
  5. pmc Tension sensing by Aurora B kinase is independent of survivin-based centromere localization
    Christopher S Campbell
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92037, USA
    Nature 497:118-21. 2013
    ..These results suggest that activation of Aurora B kinase by clustering either on chromatin or on microtubules is sufficient for chromosome biorientation...
  6. pmc Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore
    Julien Espeut
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92037, USA
    J Cell Biol 196:469-82. 2012
    ..We propose that microtubule binding by KNL-1 functions in checkpoint silencing by sensing microtubules attached to kinetochores and relaying their presence to eliminate generation of the checkpoint signal...
  7. ncbi request reprint The conserved KMN network constitutes the core microtubule-binding site of the kinetochore
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East, Room 3052, La Jolla, CA 92093, USA
    Cell 127:983-97. 2006
    ..Based on these findings, we propose that the conserved KMN network constitutes the core microtubule-binding site of the kinetochore...
  8. pmc A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
    Genes Dev 18:2255-68. 2004
    ..Thus, this protein network is a conserved constituent of the outer kinetochore, and the functions defined by our analysis in C. elegans are likely to be widely relevant...
  9. pmc A kinetochore-independent mechanism drives anaphase chromosome separation during acentrosomal meiosis
    Julien Dumont
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093 0653, USA
    Nat Cell Biol 12:894-901. 2010
    ..We suggest that separation is controlled by a meiosis-specific chromosomal domain to coordinate cohesin removal and chromosome segregation...
  10. pmc Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly
    Molly M Lettman
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Dev Cell 25:284-98. 2013
    ..We propose that ZYG-1 binding to the SAS-6 coiled coil recruits the SAS-6-SAS-5 complex to the mother centriole, where a ZYG-1 kinase activity-dependent step, whose target is unlikely to be SAS-6, triggers cartwheel assembly...
  11. pmc A microtubule-independent role for centrosomes and aurora a in nuclear envelope breakdown
    Nathan Portier
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East Room 3053, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Dev Cell 12:515-29. 2007
    ..A positive feedback loop, in which an Aurora A-dependent increase in centrosome size promotes Aurora A activation, may temporally couple centrosome maturation to NEBD during mitotic entry...
  12. pmc KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 19:587-94. 2008
    ..These findings explain discrepancies in kinetochore assembly pathways between different organisms and reveal a surprising plasticity in the assembly mechanism of an essential cell division organelle...
  13. pmc Crosstalk between microtubule attachment complexes ensures accurate chromosome segregation
    Dhanya K Cheerambathur
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Science 342:1239-42. 2013
    ..Thus, the conversion from initial dynein-mediated, lateral attachments to correctly oriented, Ndc80-mediated end-coupled attachments is actively controlled. ..
  14. pmc A Bir1-Sli15 complex connects centromeres to microtubules and is required to sense kinetochore tension
    Sharsti Sandall
    Ludwig Institute for Cancer Research Department of Cellular and Molecular Medicine, CMM E Room 3052, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 127:1179-91. 2006
    ....
  15. pmc SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the gamma-tubulin-mediated addition of centriolar microtubules
    Alexander Dammermann
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 180:771-85. 2008
    ..Such a mechanism may help restrict new centriole assembly to the vicinity of preexisting parent centrioles that recruit PCM...
  16. ncbi request reprint Centriole assembly requires both centriolar and pericentriolar material proteins
    Alexander Dammermann
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Dev Cell 7:815-29. 2004
    ..Thus, both centriolar and pericentriolar material proteins contribute to centriole assembly...
  17. pmc The midbody ring scaffolds the abscission machinery in the absence of midbody microtubules
    Rebecca A Green
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093
    J Cell Biol 203:505-20. 2013
    ..Surprisingly, midbody microtubules were dispensable for both stages. These results delineate distinct steps during abscission and highlight the central role of the midbody ring, rather than midbody microtubules, in their execution. ..
  18. ncbi request reprint The CENP-F-like proteins HCP-1 and HCP-2 target CLASP to kinetochores to mediate chromosome segregation
    Iain M Cheeseman
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego School of Medicine, La Jolla, 92093, USA
    Curr Biol 15:771-7. 2005
    ..Our results establish that the key role of HCP-1/2 is to target CLASP(CLS-2) to kinetochores, and they support the recently proposed model that CLASP functions to promote the polymerization of kinetochore bound microtubules [4]...
  19. pmc Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling
    Yinghui Mao
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093, USA
    J Cell Biol 170:873-80. 2005
    ..Together, this reveals that CENP-E is the signal transducing linker responsible for silencing BubR1-dependent mitotic checkpoint signaling through its capture at kinetochores of spindle microtubules...
  20. ncbi request reprint Expression and imaging of fluorescent proteins in the C. elegans gonad and early embryo
    Rebecca A Green
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Methods Cell Biol 85:179-218. 2008
    ....
  21. pmc Systematic analysis in Caenorhabditis elegans reveals that the spindle checkpoint is composed of two largely independent branches
    Anthony Essex
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 20:1252-67. 2009
    ..We discuss the rationale for a bipartite checkpoint mechanism in which a core Mad1(MDF-1)/Mad2(MDF-2) signal generated at kinetochores is integrated with a separate cytoplasmic Mad3(SAN-1)/BUB-3-based pathway...
  22. pmc Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cells
    Reto Gassmann
    Ludwig Institute for Cancer Research Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 24:957-71. 2010
    ..In the absence of Spindly, a second mechanism silences the checkpoint; this mechanism is likely evolutionarily ancient, as fungi and higher plants lack kinetochore dynein...
  23. pmc A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex
    Reto Gassmann
    Ludwig Institute for Cancer Research Dept of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 22:2385-99. 2008
    ....
  24. pmc A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint
    Mark W Moyle
    Ludwig Institute for Cancer Research and 2 Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92037
    J Cell Biol 204:647-57. 2014
    ..These results delineate an interaction between BUB-1 and MAD-1 that targets MAD-1-MAD-2 complexes to kinetochores and is essential for spindle checkpoint signaling. ..
  25. pmc Affinity purification of protein complexes in C. elegans
    Esther Zanin
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, USA
    Methods Cell Biol 106:289-322. 2011
    ..In addition, we describe methods to test specificity of antibodies, strategies for optimizing starting material, and approaches to distinguish specific from non-specific interactions...
  26. pmc An inverse relationship to germline transcription defines centromeric chromatin in C. elegans
    Reto Gassmann
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92037, USA
    Nature 484:534-7. 2012
    ..These findings link centromere identity to transcription and shed light on the evolutionary plasticity of centromeres...
  27. pmc The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly
    Lindsay Lewellyn
    Department of Cellular and Molecular Medicine, Biomedical Sciences Graduate Program, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 193:155-69. 2011
    ..Therefore, in contrast to the linear midzone assembly pathway, centralspindlin and the CPC make independent contributions to control transformation of the sheet-like equatorial band into a ribbon-like contractile ring at the furrow tip...
  28. ncbi request reprint Anillin and the septins promote asymmetric ingression of the cytokinetic furrow
    Amy Shaub Maddox
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine UCSD, CMM East Rm 3053, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Dev Cell 12:827-35. 2007
    ....
  29. ncbi request reprint "Holo"er than thou: chromosome segregation and kinetochore function in C. elegans
    Paul S Maddox
    CMM East, Rm 3071G, Ludwig Institute for Cancer Research, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Chromosome Res 12:641-53. 2004
    ..elegans. In addition to reviewing what is known about kinetochore function, chromosome structure, and chromosome movement, we consider the consequences of the specialized holocentric architecture on chromosome segregation...
  30. pmc Analyzing the effects of delaying aster separation on furrow formation during cytokinesis in the Caenorhabditis elegans embryo
    Lindsay Lewellyn
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 21:50-62. 2010
    ....
  31. pmc A role for Rab5 in structuring the endoplasmic reticulum
    Anjon Audhya
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 178:43-56. 2007
    ..RAB-5 and YOP-1/RET-1 also control the kinetics of nuclear envelope disassembly, which suggests an important role for the morphology of the peripheral ER in this process...
  32. pmc A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans
    Anjon Audhya
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 171:267-79. 2005
    ..Cumulatively, our results suggest that CAR-1 functions with CGH-1 to regulate a specific set of maternally loaded RNAs that is required for anaphase spindle structure and cytokinesis...
  33. ncbi request reprint Distinct roles for two C. elegans anillins in the gonad and early embryo
    Amy Shaub Maddox
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Development 132:2837-48. 2005
    ..Our results highlight specialization of divergent anillin family proteins in the C. elegans life cycle and reveal conserved roles for this protein family in organizing syncytial structures and cortical contractility...
  34. ncbi request reprint Kinetochore-spindle microtubule interactions during mitosis
    Susan L Kline-Smith
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Dr, CMM East, Rm 3080, La Jolla, California 92093, USA
    Curr Opin Cell Biol 17:35-46. 2005
    ....
  35. pmc PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression
    Wen Liu
    Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nature 466:508-12. 2010
    ..Thus, the identification and characterization of an H4K20me1 demethylase, PHF8, has revealed an intimate link between this enzyme and two distinct events in cell cycle progression...
  36. pmc Spatial control of phospholipid flux restricts endoplasmic reticulum sheet formation to allow nuclear envelope breakdown
    Shirin Bahmanyar
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 28:121-6. 2014
    ....
  37. pmc An FHA domain-mediated protein interaction network of Rad53 reveals its role in polarized cell growth
    Marcus B Smolka
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 175:743-53. 2006
    ..Collectively, the results suggest a novel function of Rad53 in the regulation of polarized cell growth in response to DNA replication stress...
  38. ncbi request reprint Analysis of kinetochore assembly and function in Caenorhabditis elegans embryos and human cells
    Reto Gassmann
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, UCSD School of Medicine, CMM East, Rm 3080, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Methods 41:177-89. 2007
    ..Here, we discuss basic methods, largely based on fluorescence imaging, to study kinetochore structure and function in these two metazoan model systems...
  39. pmc Hierarchical assembly of the eggshell and permeability barrier in C. elegans
    Sara K Olson
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 198:731-48. 2012
    ..These findings delineate the hierarchy of eggshell assembly and define key molecular mechanisms at each step...
  40. pmc Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay
    Paul S Maddox
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, and Biomedical Sciences Graduate Program, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 103:15097-102. 2006
    ....
  41. doi request reprint A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis
    Esther Zanin
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA Center for Integrated Protein Science CIPSM, Department Biology II, Ludwig Maximilians University, Munich, 82152 Planegg Martinsried, Germany
    Dev Cell 26:496-510. 2013
    ..These results identify the major GAP restraining RhoA during cell division and delineate the relative contributions of RhoA flux and centrosomal asters in controlling RhoA zone dimensions...
  42. pmc A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue
    Rebecca A Green
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, 92093, USA
    Cell 145:470-82. 2011
    ....
  43. pmc Linked in: formation and regulation of microtubule attachments during chromosome segregation
    Dhanya K Cheerambathur
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA Electronic address
    Curr Opin Cell Biol 26:113-22. 2014
    ..Here we review recent advances in mechanistic analysis of microtubule attachment formation and regulation. ..
  44. pmc Acute drug treatment in the early C. elegans embryo
    Ana Carvalho
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e24656. 2011
    ..This method should also open new avenues of investigation by allowing profiling and specificity-testing of inhibitors through comparison with genome-wide phenotypic datasets...
  45. doi request reprint A two-step mechanism for epigenetic specification of centromere identity and function
    Daniele Fachinetti
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, California 92093, USA
    Nat Cell Biol 15:1056-66. 2013
    ....
  46. pmc An integrated mechanobiochemical feedback mechanism describes chromosome motility from prometaphase to anaphase in mitosis
    Jian Liu
    Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 105:13752-7. 2008
    ..Predictions of our model can be tested experimentally...
  47. pmc Dominant mutations in S. cerevisiae PMS1 identify the Mlh1-Pms1 endonuclease active site and an exonuclease 1-independent mismatch repair pathway
    Catherine E Smith
    Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, California, United States of America
    PLoS Genet 9:e1003869. 2013
    ..These studies establish that the Mlh1-Pms1 endonuclease is required for MMR in a previously uncharacterized Exo1-independent MMR pathway. ..
  48. pmc Rapid de novo centromere formation occurs independently of heterochromatin protein 1 in C. elegans embryos
    Karen W Y Yuen
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92037, USA
    Curr Biol 21:1800-7. 2011
    ..elegans embryos in an HP1-independent manner and suggest that, rather than being a prerequisite, HP1-dependent heterochromatin antagonizes de novo centromerization...
  49. pmc Uncoordinated loss of chromatid cohesion is a common outcome of extended metaphase arrest
    Deanna Stevens
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 6:e22969. 2011
    ..These findings have important implications for analysis of mitotic phenotypes in human cells and for development of anti-mitotic chemotherapeutic approaches in the treatment of cancer...
  50. ncbi request reprint Cell division: AAAtacking the mitotic spindle
    Iain M Cheeseman
    CMM East, Rm 3071G, Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
    Curr Biol 14:R70-2. 2004
    ..But how is this spindle broken down once chromosome segregation is complete? New evidence implicates a highly conserved AAA-ATPase in spindle disassembly at the end of mitosis...
  51. ncbi request reprint A combined approach for the localization and tandem affinity purification of protein complexes from metazoans
    Iain M Cheeseman
    Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
    Sci STKE 2005:pl1. 2005
    ..The LAP strategy should be widely applicable to the characterization of protein function in cellular and developmental contexts in various metazoans...
  52. doi request reprint Structural memory in the contractile ring makes the duration of cytokinesis independent of cell size
    Ana Carvalho
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, CMM East 3080, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cell 137:926-37. 2009
    ..The scalability of constriction may facilitate coordination of mitotic events and cytokinesis when cell size, and hence the distance traversed by the ring, varies during embryogenesis and in other contexts...
  53. pmc Mlh2 is an accessory factor for DNA mismatch repair in Saccharomyces cerevisiae
    Christopher S Campbell
    Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, California, United States of America
    PLoS Genet 10:e1004327. 2014
    ..cerevisiae Mlh2 protein and the mammalian PMS1 protein are homologs. These results support a hypothesis that Mlh1-Mlh2 is a non-essential accessory factor that acts to enhance the activity of Mlh1-Pms1. ..
  54. pmc A mechanobiochemical mechanism for monooriented chromosome oscillation in mitosis
    Jian Liu
    Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093 0374, USA
    Proc Natl Acad Sci U S A 104:16104-9. 2007
    ..We suggest potential molecular players, whose perturbation may allow direct experimental tests of the model...
  55. pmc Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates
    Hans Hombauer
    Ludwig Institute for Cancer Research, University of California School of Medicine, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Cell 147:1040-53. 2011
    ..These findings suggest the presence of replication machinery-coupled and -independent pathways for mispair recognition by Msh2-Msh6, which direct formation of superstoichiometric Mlh1-Pms1 foci that represent sites of active MMR...
  56. pmc The wages of CIN
    Karen W Yuen
    Ludwig Institute for Cancer Research, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 180:661-3. 2008
    ..Their finding that induced aneuploidy is rapidly selected against suggests that the persistence of aneuploid cells in tumors requires not only chromosome missegregation but also additional, as yet poorly defined events...
  57. pmc Inhibition of Rac by the GAP activity of centralspindlin is essential for cytokinesis
    Julie C Canman
    Institute for Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Science 322:1543-6. 2008
    ..Thus, inactivation of Rac by centralspindlin functions in parallel with RhoA activation to drive contractile ring constriction during cytokinesis...
  58. ncbi request reprint Microtubule cytoskeleton: a new twist at the end
    Torsten Wittmann
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Curr Biol 15:R126-9. 2005
    ..Two recent papers on one such protein, CLIP-170, provide new insights into the cellular functions of +TIPs as well as the mechanism by which they track microtubule ends...
  59. pmc Meiotic double-strand breaks uncover and protect against mitotic errors in the C. elegans germline
    Deanna Stevens
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92037, USA
    Curr Biol 23:2400-6. 2013
    ..These findings reveal that the process of initiating meiotic recombination inherently selects against nuclei with abnormal chromosomal content generated by mitotic errors, thereby ensuring the genomic integrity of gametes...
  60. ncbi request reprint The minus end in sight
    Alexander Dammermann
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, CMM East, Rm 3080, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Curr Biol 13:R614-24. 2003
    ..These mechanisms are used in different biological contexts to generate the diversity of organized microtubule arrays in cells...
  61. pmc What the hec is up with mouse oocyte meiosis?
    Christopher S Campbell
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92037, USA
    Dev Cell 25:3-4. 2013
    ....
  62. ncbi request reprint Molecular architecture of the kinetochore-microtubule interface
    Iain M Cheeseman
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Rev Mol Cell Biol 9:33-46. 2008
    ..Recent studies have identified more than 80 kinetochore components, and are revealing how these proteins are organized into the higher order kinetochore structure, as well as how they function to achieve proper chromosome segregation...
  63. ncbi request reprint The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly
    Claire E Walczak
    Medical Sciences, Indiana University, Bloomington, IN 47405, USA
    Curr Biol 12:1885-9. 2002
    ..These results suggest that centromere bound XKCM1 has an important role in chromosome positioning on the spindle...
  64. pmc KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans
    Arshad Desai
    Max Planck Institute for Molecular Cell Biology and Genetics MPI CBG, Dresden 01307, Germany
    Genes Dev 17:2421-35. 2003
    ..Thus, KNL-1 plays a central role in translating the initiation of kinetochore assembly by CeCENP-A and CeCENP-C into the formation of a functional microtubule-binding interface...
  65. ncbi request reprint Cell division: feeling tense enough?
    Iain M Cheeseman
    Nature 428:32-3. 2004
  66. pmc Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion
    J Richard McIntosh
    Department of M C D Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Cell 135:322-33. 2008
    ..These analyses define a ring-independent mechanism for harnessing microtubule dynamics directly to chromosome movement...
  67. ncbi request reprint An essential function of the C. elegans ortholog of TPX2 is to localize activated aurora A kinase to mitotic spindles
    Nurhan Ozlu
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden 01307, Germany
    Dev Cell 9:237-48. 2005
    ..Thus, activation and targeting of Aurora A appears to be an ancient and conserved function of TPX2 that plays a central role in mitotic spindle assembly...
  68. ncbi request reprint Analysis of microtubule polymerization in vitro and during the cell cycle in Xenopus egg extracts
    Priya Prakash Budde
    Howard Hughes Medical Institute, Department of Microbiology and Molecular Biology, Tufts University School of Medicine, MA 02111, USA
    Methods 38:29-34. 2006
    ..These methods can be used to analyze the effect of a protein of interest on the microtubule cytoskeleton...
  69. ncbi request reprint The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 8:446-57. 2006
    ....
  70. pmc Kinetochore-microtubule interactions: the means to the end
    Tomoyuki U Tanaka
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, MSI WTB JBC Complex, Dundee, UK
    Curr Opin Cell Biol 20:53-63. 2008
    ..In this article, we review these recent studies and summarize their key findings...
  71. ncbi request reprint Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos
    Paul Maddox
    The Cell Division Group, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Curr Biol 12:1670-4. 2002
    ....
  72. doi request reprint Proteomics in Caenorhabditis elegans
    Anjon Audhya
    Department of Biomolecular Chemistry, University of Wisconsin Madison, USA
    Brief Funct Genomic Proteomic 7:205-10. 2008
    ..Here, we review proteomic approaches that are becoming part of the essential toolbox for studies of gene function in C. elegans and highlight specific examples where their application has led to important new insights...
  73. ncbi request reprint The (theoretical) yin and yang of spindle mechanics
    Arshad Desai
    Max Planck Institute of Cell Biology and Genetics, Dresden, Germany
    Dev Cell 3:465-7. 2002
    ..The "virtual" results reveal that a mixed polarity motor complex is needed to do the job...

Research Grants7

  1. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2010
    ..Elucidation of these mechanisms will contribute to our understanding of the development and progression of cancer and provide new avenues for therapeutic development. ..
  2. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2005
    ..The final aim will focus on other conserved outer kinetochore proteins to define their specific roles at the interface with spindle microtubules in vivo and to investigate their interactions with microtubules in vitro. ..
  3. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2006
    ..The final aim will focus on other conserved outer kinetochore proteins to define their specific roles at the interface with spindle microtubules in vivo and to investigate their interactions with microtubules in vitro. ..
  4. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2007
    ..The final aim will focus on other conserved outer kinetochore proteins to define their specific roles at the interface with spindle microtubules in vivo and to investigate their interactions with microtubules in vitro. ..
  5. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2009
    ..The final aim will focus on other conserved outer kinetochore proteins to define their specific roles at the interface with spindle microtubules in vivo and to investigate their interactions with microtubules in vitro. ..
  6. Kinetochore Specification and Function
    Arshad Desai; Fiscal Year: 2010
    ..The final aim will focus on other conserved outer kinetochore proteins to define their specific roles at the interface with spindle microtubules in vivo and to investigate their interactions with microtubules in vitro. ..