R J Cousins

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi request reprint A role of zinc in the regulation of gene expression
    R J Cousins
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611 0370, USA
    Proc Nutr Soc 57:307-11. 1998
  2. ncbi request reprint Metallothionein knockout and transgenic mice exhibit altered intestinal processing of zinc with uniform zinc-dependent zinc transporter-1 expression
    S R Davis
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    J Nutr 128:825-31. 1998
  3. ncbi request reprint Metallothionein expression protects against carbon tetrachloride-induced hepatotoxicity, but overexpression and dietary zinc supplementation provide no further protection in metallothionein transgenic and knockout mice
    S R Davis
    Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611 0370, USA
    J Nutr 131:215-22. 2001
  4. ncbi request reprint Dietary zinc deficiency increases uroguanylin accumulation in rat kidney
    L Cui
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611-0370, USA
    Kidney Int 59:1424-31. 2001
  5. pmc Regulation of the zinc transporter ZnT-1 by dietary zinc
    R J McMahon
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 95:4841-6. 1998
  6. ncbi request reprint Differential regulation of zinc transporter 1, 2, and 4 mRNA expression by dietary zinc in rats
    J P Liuzzi
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville FL 32611-0370, USA
    J Nutr 131:46-52. 2001
  7. pmc Modulation of intestinal gene expression by dietary zinc status: effectiveness of cDNA arrays for expression profiling of a single nutrient deficiency
    R K Blanchard
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611-0370, USA
    Proc Natl Acad Sci U S A 98:13507-13. 2001
  8. ncbi request reprint Human cysteine-rich intestinal protein: cDNA cloning and expression of recombinant protein and identification in human peripheral blood mononuclear cells
    C Khoo
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, USA
    Protein Expr Purif 9:379-87. 1997
  9. ncbi request reprint Upregulation of rat intestinal uroguanylin mRNA by dietary zinc restriction
    R K Blanchard
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, USA
    Am J Physiol 272:G972-8. 1997
  10. ncbi request reprint Effects of intracellular zinc depletion on metallothionein and ZIP2 transporter expression and apoptosis
    J Cao
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville 32611 0370, USA
    J Leukoc Biol 70:559-66. 2001

Collaborators

Detail Information

Publications35

  1. ncbi request reprint A role of zinc in the regulation of gene expression
    R J Cousins
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611 0370, USA
    Proc Nutr Soc 57:307-11. 1998
    ..Zn, without question, has important functions related to gene expression. Newer technologies applied to address these functions are providing answers relating to the importance of this micronutrient in human and animal health...
  2. ncbi request reprint Metallothionein knockout and transgenic mice exhibit altered intestinal processing of zinc with uniform zinc-dependent zinc transporter-1 expression
    S R Davis
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    J Nutr 128:825-31. 1998
    ..These results further support metallothionein as an important component for reducing the efficiency of zinc absorption at elevated zinc intakes...
  3. ncbi request reprint Metallothionein expression protects against carbon tetrachloride-induced hepatotoxicity, but overexpression and dietary zinc supplementation provide no further protection in metallothionein transgenic and knockout mice
    S R Davis
    Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611 0370, USA
    J Nutr 131:215-22. 2001
    ..However, neither metallothionein overexpression nor supplemental dietary zinc provided further protection...
  4. ncbi request reprint Dietary zinc deficiency increases uroguanylin accumulation in rat kidney
    L Cui
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611-0370, USA
    Kidney Int 59:1424-31. 2001
    ....
  5. pmc Regulation of the zinc transporter ZnT-1 by dietary zinc
    R J McMahon
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 95:4841-6. 1998
    ....
  6. ncbi request reprint Differential regulation of zinc transporter 1, 2, and 4 mRNA expression by dietary zinc in rats
    J P Liuzzi
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville FL 32611-0370, USA
    J Nutr 131:46-52. 2001
    ..The relative differential in regulation by zinc is ZnT-2 > ZnT-1 > ZnT-4. These data provide evidence that, in an animal model, zinc transporter expression is responsive to zinc under physiologically relevant conditions...
  7. pmc Modulation of intestinal gene expression by dietary zinc status: effectiveness of cDNA arrays for expression profiling of a single nutrient deficiency
    R K Blanchard
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611-0370, USA
    Proc Natl Acad Sci U S A 98:13507-13. 2001
    ..Overall, these data support the effectiveness of cDNA array expression profiling to investigate the pleiotropic effects of specific nutrients and may provide an approach to establishing markers for assessment of nutritional status...
  8. ncbi request reprint Human cysteine-rich intestinal protein: cDNA cloning and expression of recombinant protein and identification in human peripheral blood mononuclear cells
    C Khoo
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, USA
    Protein Expr Purif 9:379-87. 1997
    ..The results are consistent with a functional role for CRIP in proliferation/differentiation of specific cell types, particularly those associated with host defense...
  9. ncbi request reprint Upregulation of rat intestinal uroguanylin mRNA by dietary zinc restriction
    R K Blanchard
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611, USA
    Am J Physiol 272:G972-8. 1997
    ..These results also suggest a potential mechanism to explain, at least in part, the beneficial effects of zinc supplementation for secretory diarrhea prevalent in many areas of the world...
  10. ncbi request reprint Effects of intracellular zinc depletion on metallothionein and ZIP2 transporter expression and apoptosis
    J Cao
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville 32611 0370, USA
    J Leukoc Biol 70:559-66. 2001
    ..These data show that metallothionein and ZIP2 expression are inversely related during zinc depletion and that apoptosis is concurrent with these changes...
  11. pmc Purification and properties of rat cysteine-rich intestinal protein
    C Khoo
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611
    Biochem J 299:445-50. 1994
    ..The metal-binding properties of CRIP are consistent with proposed zinc-related functions for this cytoplasmic protein, which is expressed in the small intestine during the postnatal period...
  12. ncbi request reprint cDNA array analysis identifies thymic LCK as upregulated in moderate murine zinc deficiency before T-lymphocyte population changes
    J B Moore
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 131:3189-96. 2001
    ..Results demonstrate that 3 wk of dietary zinc insufficiency can alter specific thymic mRNA and protein abundances before alterations occur in thymocyte development as detectable by FACS analysis...
  13. pmc A global view of the selectivity of zinc deprivation and excess on genes expressed in human THP-1 mononuclear cells
    Robert J Cousins
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, and Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611 0370, USA
    Proc Natl Acad Sci U S A 100:6952-7. 2003
    ..Categorization by function revealed numerous genes needed for host defense were among those identified as zinc responsive, including cytokine receptors and genes associated with amplification of the Th1 immune response...
  14. pmc Plenary Lecture 2: Transcription factors, regulatory elements and nutrient-gene communication
    Robert J Cousins
    Center for Nutritional Sciences, Food Science and Human Nutrition Department, College of Agricultural and Life Sciences and Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida, USA
    Proc Nutr Soc 69:91-4. 2010
    ..This process in turn influences signalling molecules that regulate gene expression. The examples provided in the present review point to a new level of complexity in understanding nutrient-gene communication...
  15. ncbi request reprint Mammalian zinc transporters
    Juan P Liuzzi
    Nutrition Genomics Laboratory and Center for Nutritional Sciences, University of Florida, Gainesville, Florida, 32611 0370, USA
    Annu Rev Nutr 24:151-72. 2004
    ..Both the ZnT and Zip transporter families exhibit unique tissue-specific expression, differential responsiveness to dietary zinc deficiency and excess, and differential responsiveness to physiologic stimuli via hormones and cytokines...
  16. ncbi request reprint Regulation of zinc metabolism and genomic outcomes
    Robert J Cousins
    Nutritional Genomics Laboratory, Food Science and Human Nutrition, Department for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 133:1521S-6S. 2003
    ..Collectively, these findings provide a genomic footprint upon which to address the biological and clinical significance of zinc and new avenues for status assessment...
  17. ncbi request reprint Mammalian zinc transport, trafficking, and signals
    Robert J Cousins
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611 0370, USA
    J Biol Chem 281:24085-9. 2006
  18. pmc Cloning and initial characterization of the promoter region of the rat cysteine-rich intestinal protein gene
    C W Levenson
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611
    Biochem J 303:731-6. 1994
    ..These experiments provide the initial data required to explore further the regulation of this tissue-specific developmentally regulated Zn(2+)-finger protein...
  19. ncbi request reprint Mammalian zinc transporters
    R J McMahon
    Center for Nutritional Sciences and Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    J Nutr 128:667-70. 1998
    ..Collectively, description of transporters that influence cellular zinc uptake and efflux will provide a clearer understanding of the molecular events that regulate zinc absorption and homeostasis...
  20. pmc Responsive transporter genes within the murine intestinal-pancreatic axis form a basis of zinc homeostasis
    Juan P Liuzzi
    Nutritional Genomics Laboratory, Center for Nutritional Sciences, and Small Animal Clinical Sciences Department, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 101:14355-60. 2004
    ..The results show that Zn transporter expression in the intestinal-pancreatic axis is a component of the homeostatic regulation of this micronutrient...
  21. pmc Dietary zinc modulates gene expression in murine thymus: results from a comprehensive differential display screening
    J Bernadette Moore
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Proc Natl Acad Sci U S A 100:3883-8. 2003
    ..Several genes involved in T cell development were identified as regulated by zinc and will be targets to evaluate the effects of zinc on immune function...
  22. doi request reprint Mammalian zinc transporters: nutritional and physiologic regulation
    Louis A Lichten
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 2710, USA
    Annu Rev Nutr 29:153-76. 2009
    ..Other salient findings are the involvement of ZnT2 in lactation, ZIP14 in the hypozincemia of inflammation, ZIP6, ZIP7, and ZIP10 in metastatic breast cancer, and ZnT8 in insulin processing and as an autoantigen in diabetes...
  23. pmc STAT5-glucocorticoid receptor interaction and MTF-1 regulate the expression of ZnT2 (Slc30a2) in pancreatic acinar cells
    Liang Guo
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Proc Natl Acad Sci U S A 107:2818-23. 2010
    ..The ZnT2 transporter appears to function in a physiologically responsive manner involving entero-pancreatic zinc trafficking...
  24. pmc Erythrocyte metallothionein as an index of zinc status in humans
    A Grider
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611
    Proc Natl Acad Sci U S A 87:1259-62. 1990
    ..Metallothionein levels in erythrocytes may provide a useful index upon which to assess zinc status in humans...
  25. ncbi request reprint Overexpression of CRIP in transgenic mice alters cytokine patterns and the immune response
    Lorraine Lanningham-Foster
    Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, Florida 32611, USA
    Am J Physiol Endocrinol Metab 282:E1197-203. 2002
    ..The observed responses to overexpression of the CRIP gene are consistent with a role for this LIM protein in a cellular pathway that produces an imbalance in cytokine pattern favoring Th2 cytokines...
  26. pmc Kr├╝ppel-like factor 4 regulates adaptive expression of the zinc transporter Zip4 in mouse small intestine
    Juan P Liuzzi
    Center for Nutritional Sciences and Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA
    Am J Physiol Gastrointest Liver Physiol 296:G517-23. 2009
    ..These data from experiments with mice and murine IECs demonstrate that KLF4 is induced during zinc restriction and is a transcription factor involved in adaptive regulation of the zinc transporter ZIP4...
  27. pmc Zinc supplementation of young men alters metallothionein, zinc transporter, and cytokine gene expression in leukocyte populations
    Tolunay Beker Aydemir
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Proc Natl Acad Sci U S A 103:1699-704. 2006
    ..The results obtained also show that zinc supplementation of human subjects programs specific leukocytic subsets to show enhanced cytokine expression upon activation by stimulators of immunity...
  28. pmc Zip14 (Slc39a14) mediates non-transferrin-bound iron uptake into cells
    Juan P Liuzzi
    Nutritional Genomics Laboratory, Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Proc Natl Acad Sci U S A 103:13612-7. 2006
    ..Because NTBI is commonly found in plasma of patients with hemochromatosis and transfusional iron overload, Zip14-mediated NTBI uptake may contribute to the hepatic iron loading that characterizes these diseases...
  29. pmc Zinc transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in mouse red blood cells are differentially regulated during erythroid development and by dietary zinc deficiency
    Moon Suhn Ryu
    Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    J Nutr 138:2076-83. 2008
    ..Collectively, these data suggest that the zinc transporters ZnT1, Zip8, and Zip10 are important for zinc homeostasis in erythrocytes and that ZnT1 and Zip10 respond to the dietary zinc supply...
  30. ncbi request reprint Altered zinc metabolism occurs in murine lethal milk syndrome
    D Y Lee
    Food Science and Human Nutrition Department, University of Florida, Gainesville 32611
    J Nutr 122:2233-8. 1992
    ..The greater metallothionein expression in dams of the lm genotype could be a secondary manifestation of altered tissue zinc distribution or a primary effect on a metallothionein regulatory mechanism...
  31. pmc Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response
    Juan P Liuzzi
    Nutritional Genomics Laboratory, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 102:6843-8. 2005
    ....
  32. ncbi request reprint Zinc transporters 1, 2 and 4 are differentially expressed and localized in rats during pregnancy and lactation
    Juan P Liuzzi
    Food Science and Human Nutrition Department, University of Florida, Gainesville, FL, USA
    J Nutr 133:342-51. 2003
    ..These results support a role for these transporters in maintaining an adequate zinc supply derived from the maternal diet for zinc acquisition and use by the fetus and neonate...
  33. pmc Interleukin-1beta contributes via nitric oxide to the upregulation and functional activity of the zinc transporter Zip14 (Slc39a14) in murine hepatocytes
    Louis A Lichten
    Food Science and Human Nutrition Department, Center for Nutritional Sciences, University of Florida, Gainesville, FL 32611 0370, USA
    Am J Physiol Gastrointest Liver Physiol 296:G860-7. 2009
    ..These data suggest that signaling pathways activated by NO are factors in the upregulation of Zip14, which in turn mediates hepatic zinc accumulation and hypozincemia during inflammation and sepsis...
  34. pmc Zinc transporter ZIP8 (SLC39A8) and zinc influence IFN-gamma expression in activated human T cells
    Tolunay B Aydemir
    Center for Nutritional Sciences, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL 32611, USA
    J Leukoc Biol 86:337-48. 2009
    ..ZIP8, through control of zinc transport from the lysosome, may provide a secondary level of IFN-gamma regulation in T cells...
  35. ncbi request reprint The permissive effect of zinc deficiency on uroguanylin and inducible nitric oxide synthase gene upregulation in rat intestine induced by interleukin 1alpha is rapidly reversed by zinc repletion
    Li Cui
    Food Science and Human Nutrition Department and Center for Nutritional Sciences, University of Florida, Gainesville 32611 0370, USA
    J Nutr 133:51-6. 2003
    ..Dysregulation of both genes may contribute to the severity of zinc-responsive diarrheal disease and intestinal inflammatory disease...