BRUCE MARTIN CHRISTENSEN

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Filarial worms reduce Plasmodium infectivity in mosquitoes
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Negl Trop Dis 5:e963. 2011
  2. pmc Mosquito transcriptome profiles and filarial worm susceptibility in Armigeres subalbatus
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Negl Trop Dis 4:e666. 2010
  3. pmc Distribution of Brugia malayi larvae and DNA in vector and non-vector mosquitoes: implications for molecular diagnostics
    Sara M Erickson
    Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, St Louis, Missouri, USA
    Parasit Vectors 2:56. 2009
  4. pmc Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
    George F Mayhew
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Dr, Madison Wisconsin, 53706, USA
    BMC Genomics 8:462. 2007
  5. pmc Identification and characterization of the fibrinogen-like domain of fibrinogen-related proteins in the mosquito, Anopheles gambiae, and the fruitfly, Drosophila melanogaster, genomes
    Xinguo Wang
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    BMC Genomics 6:114. 2005
  6. pmc Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin, 1656 Linden Drive, Madison, WI 53706 USA
    BMC Genomics 8:463. 2007
  7. ncbi request reprint Melanization immune responses in mosquito vectors
    Bruce M Christensen
    Department of Animal Health and Biomedical Sciences, 1656 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Trends Parasitol 21:192-9. 2005
  8. ncbi request reprint Presidential address. Classical is critical: alleviating the burden of parasitic diseases
    Bruce M Christensen
    Department of Animal Health and Biomedical Sciences, School of Veterinary Medicine, 1656 Linden Drive, University of Wisconsin Madison, Madison, Wisconsin 53706 1520, USA
    J Parasitol 90:1199-203. 2004
  9. ncbi request reprint The role of phenylalanine hydroxylase in melanotic encapsulation of filarial worms in two species of mosquitoes
    Li Chun Infanger
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Dr, Madison, WI 53706, USA
    Insect Biochem Mol Biol 34:1329-38. 2004
  10. ncbi request reprint Replication of flock house virus in three genera of medically important insects
    Ranjit Dasgupta
    Department of Animal Health and Biomedical Sciences University of Wisconsin, Madison 53706, USA
    J Med Entomol 44:102-10. 2007

Research Grants

  1. DEFENSIN GENE REGULATION AND MOSQUITO VECTOR COMPETENCE
    Bruce Christensen; Fiscal Year: 2003
  2. IMMUNE RESPONSE OF MOSQUITOES TO FILARIAL WORMS
    Bruce Christensen; Fiscal Year: 2003
  3. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
  4. Hemocyte Transcriptome and Immunity in Aedes aegypti
    BRUCE MARTIN CHRISTENSEN; Fiscal Year: 2010

Collaborators

Detail Information

Publications34

  1. pmc Filarial worms reduce Plasmodium infectivity in mosquitoes
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Negl Trop Dis 5:e963. 2011
    ..Ar. subalbatus used in this study are natural vectors of P. gallinaceum and B. pahangi and they are naturally refractory to B. malayi (melanization-based refractoriness)...
  2. pmc Mosquito transcriptome profiles and filarial worm susceptibility in Armigeres subalbatus
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Negl Trop Dis 4:e666. 2010
    ..subalbatus to B. malayi, and now we report transcriptome profiling studies of Ar. subalbatus in relation to filarial worm infection to provide information on the molecular components involved in B. pahangi susceptibility...
  3. pmc Distribution of Brugia malayi larvae and DNA in vector and non-vector mosquitoes: implications for molecular diagnostics
    Sara M Erickson
    Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, St Louis, Missouri, USA
    Parasit Vectors 2:56. 2009
    ..abstract:..
  4. pmc Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
    George F Mayhew
    Department of Pathobiological Sciences, University of Wisconsin Madison, 1656 Linden Dr, Madison Wisconsin, 53706, USA
    BMC Genomics 8:462. 2007
    ..Expressed sequence tags (ESTs) from each library were produced, annotated, and subjected to comparative analyses...
  5. pmc Identification and characterization of the fibrinogen-like domain of fibrinogen-related proteins in the mosquito, Anopheles gambiae, and the fruitfly, Drosophila melanogaster, genomes
    Xinguo Wang
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    BMC Genomics 6:114. 2005
    ..To understand the complexity of this family in insects, we analyzed FREPs in the mosquito genome and made comparisons to FREPs in the fruitfly genome...
  6. pmc Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus
    Matthew T Aliota
    Department of Pathobiological Sciences, University of Wisconsin, 1656 Linden Drive, Madison, WI 53706 USA
    BMC Genomics 8:463. 2007
    ..We have initiated transcriptome profiling studies in Ar. subalbatus to identify molecular components involved in B. malayi refractoriness...
  7. ncbi request reprint Melanization immune responses in mosquito vectors
    Bruce M Christensen
    Department of Animal Health and Biomedical Sciences, 1656 Linden Drive, University of Wisconsin, Madison, WI 53706, USA
    Trends Parasitol 21:192-9. 2005
    ....
  8. ncbi request reprint Presidential address. Classical is critical: alleviating the burden of parasitic diseases
    Bruce M Christensen
    Department of Animal Health and Biomedical Sciences, School of Veterinary Medicine, 1656 Linden Drive, University of Wisconsin Madison, Madison, Wisconsin 53706 1520, USA
    J Parasitol 90:1199-203. 2004
  9. ncbi request reprint The role of phenylalanine hydroxylase in melanotic encapsulation of filarial worms in two species of mosquitoes
    Li Chun Infanger
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Dr, Madison, WI 53706, USA
    Insect Biochem Mol Biol 34:1329-38. 2004
    ..Our data suggest that PAH is required for a fully functional melanotic encapsulation response in both mosquito vectors...
  10. ncbi request reprint Replication of flock house virus in three genera of medically important insects
    Ranjit Dasgupta
    Department of Animal Health and Biomedical Sciences University of Wisconsin, Madison 53706, USA
    J Med Entomol 44:102-10. 2007
    ..Our results indicate that FHV has uniquely broad insect host range and that the virus can be used to study virus host interactions in a variety of medically important insects...
  11. ncbi request reprint Flock house virus replicates and expresses green fluorescent protein in mosquitoes
    Ranjit Dasgupta
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    J Gen Virol 84:1789-97. 2003
    ..This demonstrates the potential of FHV-based vectors for expression of foreign genes in mosquitoes and possibly other insects...
  12. ncbi request reprint Mosquito innate immunity: involvement of beta 1,3-glucan recognition protein in melanotic encapsulation immune responses in Armigeres subalbatus
    Xinguo Wang
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison WI 53706, USA
    Mol Biochem Parasitol 139:65-73. 2005
    ..subalbatus. These results suggest that AsGRP has the capacity to bind to a variety of pathogens, functions as a pattern recognition receptor, and is required for effective melanotic encapsulation immune responses in Ar. subalbatus...
  13. ncbi request reprint Beta 1, 3-glucan recognition protein from the mosquito, Armigeres subalbatus, is involved in the recognition of distinct types of bacteria in innate immune responses
    Xinguo Wang
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell Microbiol 8:1581-90. 2006
    ..These results demonstrate that AsGRP is selective in its affinity to different bacteria and; therefore, plays a role in the antibacterial immune response of mosquitoes...
  14. pmc Mosquito infection responses to developing filarial worms
    Sara M Erickson
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Negl Trop Dis 3:e529. 2009
    ..malayi in Ae. aegypti. The possibility of LF parasites evading mosquito immune responses during successful development is discussed...
  15. ncbi request reprint Rapid phagocytosis and melanization of bacteria and Plasmodium sporozoites by hemocytes of the mosquito Aedes aegypti
    Jullán F Hillyer
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, Wisconsin 53706, USA
    J Parasitol 89:62-9. 2003
    ..The magnitude and speed of the cellular response suggest that hemocytes, acting in concert with the humoral immune response, are the main force driving the battle against foreign invaders...
  16. pmc Evaluation of the function of a type I peritrophic matrix as a physical barrier for midgut epithelium invasion by mosquito-borne pathogens in Aedes aegypti
    Nobutaka Kato
    Department of Pathobiological Sciences, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Vector Borne Zoonotic Dis 8:701-12. 2008
    ..Finally, the absence of the PM does not affect reproductive fitness, as measured by the number and viability of eggs oviposited...
  17. ncbi request reprint Hemocyte-mediated phagocytosis and melanization in the mosquito Armigeres subalbatus following immune challenge by bacteria
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Cell Tissue Res 313:117-27. 2003
    ..These studies implicate hemocytes as the primary line of defense against bacteria...
  18. ncbi request reprint Mosquito glucosamine-6-phosphate N-acetyltransferase: cDNA, gene structure and enzyme kinetics
    Nobutaka Kato
    Department of Animal Health and Biomedical Sciences, 1656 Linden Dr University of Wisconsin Madison, Madison, WI 53706, USA
    Insect Biochem Mol Biol 35:637-46. 2005
    ..The Km value of recombinant GNA for glucosamine-6-phosphate was 330 microM and the Km for acetyl-CoA was 500 microM...
  19. ncbi request reprint Mosquito phenoloxidase and defensin colocalize in melanization innate immune responses
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, WI 53706, USA
    J Histochem Cytochem 53:689-98. 2005
    ....
  20. ncbi request reprint The antibacterial innate immune response by the mosquito Aedes aegypti is mediated by hemocytes and independent of Gram type and pathogenicity
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Microbes Infect 6:448-59. 2004
    ..melanization responses are complex and independent of bacterial Gram type and pathogenicity. These data provide further evidence that hemocytes are central to the immune response against prokaryotes...
  21. pmc Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus
    Lyric C Bartholomay
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Infect Immun 72:4114-26. 2004
    ..The sequences and annotations presented in this paper have been submitted to GenBank under accession numbers AY 431103 to AY 433788 (Aedes aegypti) and AY 439334 to AY 441440 (Armigeres subalbatus)...
  22. ncbi request reprint Characterization of hemocytes from the yellow fever mosquito, Aedes aegypti
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, 53706, USA
    Histochem Cell Biol 117:431-40. 2002
    ..These results have begun to form a knowledge base for our ongoing studies on the function of Ae. aegypti hemocytes, and their involvement in controlling infections...
  23. ncbi request reprint Regulatory mechanisms of chitin biosynthesis and roles of chitin in peritrophic matrix formation in the midgut of adult Aedes aegypti
    Nobutaka Kato
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive Madison, WI 53706, USA
    Insect Biochem Mol Biol 36:1-9. 2006
    ....
  24. ncbi request reprint Age-associated mortality in immune challenged mosquitoes (Aedes aegypti) correlates with a decrease in haemocyte numbers
    JULIAN F HILLYER
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, 1656 Linden Drive, Madison, WI 53706, USA
    Cell Microbiol 7:39-51. 2005
    ..These data also call for caution in the analysis and interpretation of experimental results when mosquito age has not been closely monitored. Lastly, a model for haemocyte function is presented...
  25. ncbi request reprint Culex pipiens pipiens: characterization of immune peptides and the influence of immune activation on development of Wuchereria bancrofti
    Lyric C Bartholomay
    Department of Animal Health and Biomedical Sciences, University of Wisconsin Madison, Madison, WI, USA
    Mol Biochem Parasitol 130:43-50. 2003
    ..Sequences and transcriptional profiles for these peptides are reported. The discrepancy between these results and those reported for the model parasite, Brugia malayi, in the mosquito Aedes aegypti are discussed...
  26. pmc Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins as dopachrome-conversion enzymes
    Qian Han
    Department of Veterinary Pathobiology, University of Illinois at Urbana Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, U S A
    Biochem J 368:333-40. 2002
    ..Results from this study provide the groundwork towards a better understanding of the physiological roles of the Drosophila yellow gene family...
  27. pmc Functional expression and characterization of Aedes aegypti dopachrome conversion enzyme
    Jianmin Fang
    Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana Champaign, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
    Biochem Biophys Res Commun 290:287-93. 2002
    ..The DCE sequence data obtained in our previous study, together with results of functional expression and biochemical characterization achieved in this study, provide a necessary reference for the study of other insect DCEs...
  28. pmc Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes
    Robert M Waterhouse
    Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK
    Science 316:1738-43. 2007
    ..These dynamics reflect in part continuous readjustment between accommodation and rejection of pathogens and suggest how innate immunity may have evolved...
  29. pmc Persistence of Brugia malayi DNA in vector and non-vector mosquitoes: implications for xenomonitoring and transmission monitoring of lymphatic filariasis
    Peter Fischer
    Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Am J Trop Med Hyg 76:502-7. 2007
    ....
  30. ncbi request reprint Purification and primary structural characterization of prophenoloxidases from Aedes aegypti larvae
    Junsuo S Li
    Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802, USA
    Insect Biochem Mol Biol 35:1269-83. 2005
    ..Results of this study provide a basis for developing a comprehensive understanding of structure/function relationships of individual proPOs in mosquitoes...
  31. ncbi request reprint Proteomic analysis of N-glycosylation in mosquito dopachrome conversion enzyme
    Junsuo S Li
    Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA
    Proteomics 7:2557-69. 2007
    ..Results of this study provide an interesting example in terms of potential complexity of the glycopeptide CID fragmentation pattern...
  32. pmc Molecular characterization of Aegyptianella pullorum (Rickettsiales, Anaplasmataceae)
    Yasuko Rikihisa
    Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Columbus, Ohio 43210, USA
    J Clin Microbiol 41:5294-7. 2003
    ..A specific PCR test was developed to analyze A. pullorum DNA. Phylogenic analysis revealed A. pullorum is most closely related to Anaplasma spp...
  33. ncbi request reprint Flight muscle-specific expression of act88F: GFP in transgenic Culex quinquefasciatus Say (Diptera: Culicidae)
    Margaret L Allen
    Entomology Department, University of California, Riverside, CA 92521, USA
    Parasitol Int 53:307-14. 2004
    ..Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles...
  34. ncbi request reprint Reproductive costs of the immune response of the autogenous mosquito Culex pipiens molestus against inoculated Dirofilaria immitis
    Marta Santa-Ana
    Centro de Estudos da Macaronésia, Universidade da Madeira, Funchal, Portugal
    Vector Borne Zoonotic Dis 7:541-6. 2007
    ..The ovaries from mosquitoes undergoing melanotic encapsulation developed more eggs than those that could not melanize the mf. Possible explanations are discussed in this article...

Research Grants4

  1. DEFENSIN GENE REGULATION AND MOSQUITO VECTOR COMPETENCE
    Bruce Christensen; Fiscal Year: 2003
    ..aegypti and Cx. pipiens. Studies with Cx. Pipiens and W. bancrofti are possible because of a collaborative arrangement with researchers in Egypt. ..
  2. IMMUNE RESPONSE OF MOSQUITOES TO FILARIAL WORMS
    Bruce Christensen; Fiscal Year: 2003
    ..abstract_text> ..
  3. CHITIN BIOSYNTHESIS AND THE MOSQUITO PERITROPHIC MATRIX
    Bruce Christensen; Fiscal Year: 2007
    ....
  4. Hemocyte Transcriptome and Immunity in Aedes aegypti
    BRUCE MARTIN CHRISTENSEN; Fiscal Year: 2010
    ..All data resulting from microarray analyses, including experimental protocols used, will be made publicly available on the interactive website that contains our hemocyte EST data. ..