John E Butler

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. doi request reprint Immunoglobulins, antibody repertoire and B cell development
    J E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA, USA
    Dev Comp Immunol 33:321-33. 2009
  2. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA Electronic address
    Dev Comp Immunol 65:340-51. 2016
  3. doi request reprint Porcine reproductive and respiratory syndrome (PRRS): an immune dysregulatory pandemic
    J E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    Immunol Res 59:81-108. 2014
  4. pmc The porcine antibody repertoire: variations on the textbook theme
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa Iowa City, IA, USA
    Front Immunol 3:153. 2012
  5. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XIV. Highly restricted IGKV gene usage parallels the pattern seen with IGLV and IGHV
    J E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Immunol 55:329-36. 2013
  6. doi request reprint Antibody repertoire development in fetal and neonatal piglets XXI. Usage of most VH genes remains constant during fetal and postnatal development
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    Mol Immunol 49:483-94. 2011
  7. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer's patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance
    John E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 187:5141-9. 2011
  8. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. XIII. Hybrid VH genes and the preimmune repertoire revisited
    John E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 177:5459-70. 2006
  9. ncbi request reprint Development of the neonatal B and T cell repertoire in swine: implications for comparative and veterinary immunology
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, The University of Iowa, Iowa City, 52242, USA
    Vet Res 37:417-41. 2006
  10. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. IX. Three pathogen-associated molecular patterns act synergistically to allow germfree piglets to respond to type 2 thymus-independent and thymus-dependent antigens
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 175:6772-85. 2005

Detail Information

Publications47

  1. doi request reprint Immunoglobulins, antibody repertoire and B cell development
    J E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA, USA
    Dev Comp Immunol 33:321-33. 2009
    ..This chapter traces the study of the humoral immune system of this species through its various eras of discovery and compiles the results in tables and figures that should be a useful reference for educators and investigators...
  2. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA Electronic address
    Dev Comp Immunol 65:340-51. 2016
    ..Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum. ..
  3. doi request reprint Porcine reproductive and respiratory syndrome (PRRS): an immune dysregulatory pandemic
    J E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    Immunol Res 59:81-108. 2014
    ....
  4. pmc The porcine antibody repertoire: variations on the textbook theme
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa Iowa City, IA, USA
    Front Immunol 3:153. 2012
    ..In swine we believe such duplications within the genome have very limited functional significance and their occurrence is therefore overrated...
  5. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XIV. Highly restricted IGKV gene usage parallels the pattern seen with IGLV and IGHV
    J E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Immunol 55:329-36. 2013
    ....
  6. doi request reprint Antibody repertoire development in fetal and neonatal piglets XXI. Usage of most VH genes remains constant during fetal and postnatal development
    John E Butler
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    Mol Immunol 49:483-94. 2011
    ..Therefore in swine a small number of VH genes shape the antibody repertoire throughout life questioning the need for extensive VH polygeny...
  7. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer's patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance
    John E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 187:5141-9. 2011
    ....
  8. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. XIII. Hybrid VH genes and the preimmune repertoire revisited
    John E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 177:5459-70. 2006
    ..One-third of these genes are chimeric genes and their sequences suggest that the porcine VH genome developed by duplication and splicing from a small number of prototypic genes...
  9. ncbi request reprint Development of the neonatal B and T cell repertoire in swine: implications for comparative and veterinary immunology
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, The University of Iowa, Iowa City, 52242, USA
    Vet Res 37:417-41. 2006
    ..Improper development of adaptive immunity can be caused by infectious agents like the porcine reproductive and respiratory syndrome virus that causes immune dysregulation resulting in immunological injury and autoimmunity...
  10. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. IX. Three pathogen-associated molecular patterns act synergistically to allow germfree piglets to respond to type 2 thymus-independent and thymus-dependent antigens
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 175:6772-85. 2005
    ....
  11. ncbi request reprint Antibody repertoire development in swine
    John E Butler
    Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA
    Dev Comp Immunol 30:199-221. 2006
    ..Swine exemplify a situation in which evolutionary diversification of the immune system is not characteristic of an entire order or even of other related systems in the same species...
  12. ncbi request reprint The pre-immune variable kappa repertoire of swine is selectively generated from certain subfamilies of Vkappa2 and one Jkappa gene
    J E Butler
    The University of Iowa, Department of Microbiology and Interdisciplinary Immunology Program, Iowa City, IA 52242, USA
    Vet Immunol Immunopathol 108:127-37. 2005
    ..These restricted pre-immune repertoires may reflect the minimal exposure of the fetus to maternal factors and environmental antigens. The significance for swine immunology of characterizing the pre-immune repertoire is discussed...
  13. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. XVII. IgG subclass transcription revisited with emphasis on new IgG3
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 177:5480-9. 2006
    ..We hypothesize that long-hinged porcine IgG3 may be important in preadaptive responses to T cell-independent Ags similar to those described for its murine namesake...
  14. ncbi request reprint The isolator piglet: a model for studying the development of adaptive immunity
    J E Butler
    Department of Microbiology and Interdisciplinary Graduate Immunology Program, University of Iowa, 3 550 BSB, 51 Newton Rd, Iowa City, IA 52242, USA
    Immunol Res 39:33-51. 2007
    ..The value of the model to medical science in such areas as food allergy, organ transplantation, cystic fibrosis and the production of humanized antibodies for immuno-therapy is discussed...
  15. ncbi request reprint Porcine reproductive and respiratory syndrome virus subverts repertoire development by proliferation of germline-encoded B cells of all isotypes bearing hydrophobic heavy chain CDR3
    John E Butler
    Department of Microbiology, University of Iowa Medical School, 51 Newton Road, Iowa City, IA 52242, USA
    J Immunol 180:2347-56. 2008
    ..This diversion from normal repertoire development may explain the delayed immune response to PRRSV...
  16. doi request reprint Porcine IgG: structure, genetics, and evolution
    J E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, The University of Iowa, Iowa City, IA 52242, USA
    Immunogenetics 61:209-30. 2009
    ..All except IgG5(a) and IgG6(a) should bind to FcgammaRs, while all except IgG6(a) and the putative IgG5 subclass proteins should bind well to porcine FcRn, protein A, and protein G...
  17. pmc The piglet as a model for B cell and immune system development
    J E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA, United States
    Vet Immunol Immunopathol 128:147-70. 2009
    ..This review summarizes the history of the development of the piglet as a model for antibody repertoire development, thus providing a framework to guide future investigators...
  18. doi request reprint Isolator and other neonatal piglet models in developmental immunology and identification of virulence factors
    J E Butler
    Department of Microbiology, University of Iowa College of Medicine, Iowa City, IA 52242 1109, USA
    Anim Health Res Rev 10:35-52. 2009
    ..Knowledge gained from studies of adaptive immunity in isolator-reared neonatal pigs suggests that isolator piglets can be valuable in identification of virulence factors that are often masked in studies using conventional animals...
  19. pmc Comparison of the expressed porcine Vbeta and Jbeta repertoire of thymocytes and peripheral T cells
    John E Butler
    Department of Microbiology, The University of Iowa, Iowa City, IA 52242 1109, USA
    Immunology 114:184-93. 2005
    ..7 usage. These studies show that unlike porcine heavy chain V(H) genes, the occurrence and relative usage of porcine TCR-Vbeta groups resembles that of humans. Thus, highly related gene systems can individually diverge within a species...
  20. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets: XIX. Undiversified B cells with hydrophobic HCDR3s preferentially proliferate in the porcine reproductive and respiratory syndrome
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 178:6320-31. 2007
    ..We speculate that PRRSV infection generates a product that engages the BCR of naive B cells, displaying the AMVLV and similar motifs in HCDR3 and resulting in their T-independent proliferation without repertoire diversification...
  21. ncbi request reprint Summary of the Comparative Immunoglobulin Workshop (CIgW) on immunoglobulins (Igs) and Fc receptors
    J E Butler
    Department of Microbiology Immunology, The University of Iowa, Medical School, Iowa City, IA 52242, USA
    Vet Immunol Immunopathol 87:481-4. 2002
  22. doi request reprint The two suborders of chiropterans have the canonical heavy-chain immunoglobulin (Ig) gene repertoire of eutherian mammals
    John E Butler
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Dev Comp Immunol 35:273-84. 2011
    ..Data reveal that CH diversity and VDJ and CDR3 organization are similar to other eutherian mammals, although the expressed VH3 family repertoire was unusually diverse...
  23. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. IV. Switch recombination, primarily in fetal thymus, occurs independent of environmental antigen and is only weakly associated with repertoire diversification
    J E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 167:3239-49. 2001
    ....
  24. ncbi request reprint Switch recombination in fetal porcine thymus is uncoupled from somatic mutation
    J E Butler
    Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA
    Vet Immunol Immunopathol 87:307-19. 2002
    ..The latter is consistent with the gradual decline in serum IgG concentration in germ-free isolator piglets and the expression of FcRn in the porcine placenta...
  25. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-dependent and type 2 T-independent antigens
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242 1109, USA
    J Immunol 169:6822-30. 2002
    ..We further propose that Ag-driven systemic responses require both bacterial colonization and TD Ags translocated to the peritoneum...
  26. ncbi request reprint Antibody repertoire development in fetal and neonatal pigs. VII. Characterization of the preimmune kappa light chain repertoire
    John E Butler
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 173:6794-805. 2004
    ..These studies, like those of porcine Ig constant regions and MHC genes, also indicate unexpected high sequence similarity with their human counterparts despite differences in phylogeny and the mechanism of repertoire diversification...
  27. pmc Antibody repertoire development in fetal and neonatal piglets. XI. The thymic B-cell repertoire develops independently from that in blood and mesenteric lymph nodes
    Jeremy McAleer
    Department of Microbiology, The University of Iowa, Iowa City, IA 52242 1109, USA
    Immunology 114:171-83. 2005
    ....
  28. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XII. Three IGLV genes comprise 70% of the pre-immune repertoire and there is little junctional diversity
    Nancy Wertz
    Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Mol Immunol 55:319-28. 2013
    ..7 and did not change in GF piglets. Similar to the heavy chain pre-immune repertoire in this species, three IGLV genes account for ~70% of the repertoire. Unlike the heavy chain repertoire, junctional diversity was very limited...
  29. ncbi request reprint Lymphoid hyperplasia resulting in immune dysregulation is caused by porcine reproductive and respiratory syndrome virus infection in neonatal pigs
    Caitlin D Lemke
    Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 172:1916-25. 2004
    ....
  30. pmc Antibody repertoire development in fetal and neonatal piglets. XXII. λ Rearrangement precedes κ rearrangement during B-cell lymphogenesis in swine
    Xiuzhu Sun
    Department of Microbiology and Interdisciplinary Immunology Program, University of Iowa College of Medicine, Iowa City, IA, USA
    Immunology 137:149-59. 2012
    ..Based on recovery of SJC, B-cell lymphogenesis continues for at least 5 weeks postpartum...
  31. pmc Antibody repertoire development in fetal and neonatal piglets. XVI. Influenza stimulates adaptive immunity, class switch and diversification of the IgG repertoire encoded by downstream Cγ genes
    John E Butler
    Department of Microbiology, Carver College of Medicine, Iowa City, IA 52240, USA
    Immunology 138:134-44. 2013
    ..Whereas IgG3 may play an initial protective role, antibodies encoded by downstream Cγ genes with diversified repertoires are predicted to be most important in long-term protection against S-FLU...
  32. ncbi request reprint Expressed swine VH genes belong to a small VH gene family homologous to human VHIII
    J Sun
    Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242
    J Immunol 153:5618-27. 1994
    ..Analyses of individual genomic bands by single strand conformational polymorphism and sequence analysis suggest that the number of VHIII-related genes in the swine genome is < 20...
  33. doi request reprint Antibody repertoire development in fetal and neonatal piglets. XV. Porcine circovirus type 2 infection differentially affects serum IgG levels and antibodies to ORF2 in piglets free from other environmental factors
    Xiuzhu Sun
    Department of Microbiology, Carver College of Medicine, Iowa City, IA, United States
    Vaccine 31:141-8. 2012
    ..In any case, developers may have to contend with significant genetic differences in the response of piglets to PCV2...
  34. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. V. VDJ gene chimeras resembling gene conversion products are generated at high frequency by PCR in vitro
    M Sinkora
    Department of Microbiology and Interdisciplinary Immunology Program, 3 501L Bowen Science Building, 51 Newton Road, The University of Iowa, 52242, Iowa City, IA, USA
    Mol Immunol 37:1025-34. 2000
    ....
  35. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. II. Characterization of heavy chain complementarity-determining region 3 diversity in the developing fetus
    J E Butler
    Department of Microbiology and Iowa Interdisciplinary Immunology Program, University of Iowa, Iowa City, IA 52242, USA
    J Immunol 165:6999-7010. 2000
    ..These findings suggest that in the fetal piglet, highly restricted combinatorial diversity and the lack of somatic mutation are compensated by early onset of TdT activity and other mechanisms that contribute to CDR3 junctional diversity...
  36. ncbi request reprint Early ontogeny of thymocytes in pigs: sequential colonization of the thymus by T cell progenitors
    M Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic
    J Immunol 165:1832-9. 2000
    ..Finally, we observed a subset of TCRgammadelta+ thymocytes that were cycling late during their development in the thymus...
  37. ncbi request reprint Five putative subclasses of swine IgG identified from the cDNA sequences of a single animal
    I Kacskovics
    Department of Microbiology, University of Iowa, Iowa City 52242
    J Immunol 153:3565-73. 1994
    ..Genomic blots suggest that up to eight C gamma genes are present in the genome...
  38. ncbi request reprint The heterogeneity of bovine IgG2--VIII. The complete cDNA sequence of bovine IgG2a (A2) and an IgG1
    I Kacskovics
    Department of Microbiology, University of Iowa, Iowa City 52242, USA
    Mol Immunol 33:189-95. 1996
    ..We found no evidence to support the hypothesis that similarities between the CH3 domains of IgG2a(A2) and IgG1 result from gene conversion in the C-region of the bovine heavy chain locus...
  39. ncbi request reprint Solid supports in enzyme-linked immunosorbent assay and other solid-phase immunoassays
    John E Butler
    Department of Microbiology, Interdisciplinary Immunology Training Program, The University of Iowa, Iowa City, USA
    Methods Mol Med 94:333-72. 2004
    ..Empiricism, adherence to a few practical rules of thumb, and avoidance of certain "old wives tales" can be valuable in the successful development of SPIs...
  40. ncbi request reprint Characterization of porcine CD19 and anti-CD19 monoclonal antibodies
    Jishan Sun
    Department of Microbiology, The University of Iowa, Iowa City, IA 52242 1109, USA
    Mol Immunol 41:929-38. 2004
    ..coli, mAbs to the bacterially-expressed product did not recognize CD19 on porcine B cells suggesting that carbohydrate-dependent conformation may determine antigenicity...
  41. ncbi request reprint Comparative studies on the interaction of proteins with a polydimethylsiloxane elastomer. II. The comparative antigenicity of primary and secondarily adsorbed IgG1 and IgG2a and their non-adsorbed counterparts
    J E Butler
    Department of Microbiology, University of Iowa, Iowa City 52242 1109, USA
    J Mol Recognit 10:52-62. 1997
    ..The significantly great antigenicity of secondarily adsorbed IgG suggests that it may be present in near native conformation...
  42. ncbi request reprint Lymphocyte development in fetal piglets: facts and surprises
    Marek Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Doly 183, 549 22 Novy Hradek, Czech Republic
    Vet Immunol Immunopathol 108:177-84. 2005
    ..Therefore, swine present an example of two highly related receptor systems that have diverged in the same species...
  43. ncbi request reprint Prenatal development of the porcine TCR delta repertoire: dominant expression of an invariant T cell receptor Vdelta3-Jdelta3 chain
    Wolfgang Holtmeier
    Medizinische Klinik II, Division of Gastroenterology, Johann Wolfgang Goethe Universitat, Frankfurt am Main, Germany
    Eur J Immunol 34:1941-9. 2004
    ..We speculate that these gamma/delta T cells might have been selectively expanded by (self)-ligands and may have an important function throughout fetal development...
  44. ncbi request reprint B cell development and VDJ rearrangement in the fetal pig
    Marek Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, 549 22, Novy Hradek, Czech Republic
    Vet Immunol Immunopathol 87:341-6. 2002
    ..In contrast with the yolk sac and fetal liver, the proportion of in-frame VDJ rearrangements in the bone marrow correspond to a value indicative of random recombination...
  45. ncbi request reprint Antibody repertoire development in fetal and neonatal piglets. VI. B cell lymphogenesis occurs at multiple sites with differences in the frequency of in-frame rearrangements
    Marek Sinkora
    Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Doly 183, 549 22 Novy Hradek, Czech Republic
    J Immunol 170:1781-8. 2003
    ..We propose the latter to result from differential selection or a developmentally dependent change in the intrinsic mechanism of VDJ rearrangement...
  46. pmc Identification of IgF, a hinge-region-containing Ig class, and IgD in Xenopus tropicalis
    Yaofeng Zhao
    State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, People s Republic of China
    Proc Natl Acad Sci U S A 103:12087-92. 2006
    ..The IgH locus of X. tropicalis shows a 5' V(H)-D(H)-J(H)-C(mu)-C(delta)-C(chi)-C(upsilon)-C(phi) 3' organization, suggesting that the mammalian and amphibian Ig heavy-chain loci share a common ancestor...
  47. ncbi request reprint Genomic organization of the immunoglobulin light chain gene loci in Xenopus tropicalis: evolutionary implications
    Tong Qin
    State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, PR China
    Dev Comp Immunol 32:156-65. 2008
    ....