David A Brafman
Affiliation: University of California
- Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)David A Brafman
Department of Cellular and Molecular Medicine, Stem Cell Program, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093 0395, USA
Methods Mol Biol 1212:87-102. 2015..Finally, this protocol allows for the robust differentiation of NPCs into microtubule-associated protein 2 (MAP2) and β-Tubulin-III (β3T) positive neurons...
- Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactionsD A Brafman
Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, La Jolla, CA 92093, USA
Cell Death Differ 20:369-81. 2013..These data provide evidence that FN and VTN promote endoderm differentiation of hESCs through interaction with ITGA5 and ITGAV, and that ECMP-integrin interactions are required for hESC differentiation into functionally mature cells...
- Investigating the role of the extracellular environment in modulating hepatic stellate cell biology with arrayed combinatorial microenvironmentsDavid A Brafman
Department of Bioengineering, University of California, San Diego, USA
Integr Biol (Camb) 1:513-24. 2009..Our results indicate that array-based screens may provide an efficient means for identifying candidate signaling pathways to be targeted for anti-fibrotic therapies...
- Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary and rare cell populationsDavid A Brafman
Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, California, USA
Nat Protoc 7:703-17. 2012..A standard ACME screen uses the technologies previously applied to the manufacture and analysis of DNA microarrays, requires standard cell-culture facilities and can be performed from beginning to end within 5-10 days...
- Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technologyDavid A Brafman
Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
Stem Cells Dev 18:1141-54. 2009..In the future, the novel array platform and analysis procedure presented here will be applied toward the directed differentiation of hESCs and maintenance of other stem and progenitor cell populations...
- Long-term human pluripotent stem cell self-renewal on synthetic polymer surfacesDavid A Brafman
Department of Bioengineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093 0695, United States
Biomaterials 31:9135-44. 2010....
- Endogenous WNT Signaling Regulates hPSC-Derived Neural Progenitor Cell Heterogeneity and Specifies Their Regional IdentityNoel Moya
Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
Stem Cell Reports 3:1015-28. 2014..The ability to manipulate WNT signaling to generate regionally specific NPCs and neurons will be useful for studying human neural development and will greatly enhance the translational potential of hPSCs for neural-related therapies. ..
- Analysis of SOX2-expressing cell populations derived from human pluripotent stem cellsDavid A Brafman
Stem Cell Program, Department of Cellular and Molecular Medicine, UCSD, 9500 Gilman Drive, La Jolla, CA 92093 0695, USA
Stem Cell Reports 1:464-78. 2013..In AFE, we used transcriptome-wide expression analysis to identify cell surface markers with elevated expression in this population, thereby facilitating isolation and purification of this hPSC-derived cell population. ..
- Constructing stem cell microenvironments using bioengineering approachesDavid A Brafman
Department of Cellular and Molecular Medicine, Stem Cell Program, University of California at San Diego, La Jolla, California
Physiol Genomics 45:1123-35. 2013..Finally, the emerging trend of using high-throughput, combinatorial methods for the stepwise engineering of stem cell microenvironments will be explored. ..