Timothy R Billiar

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint Making progress in an enlightened era: opportunities and obstacles
    Timothy R Billiar
    Presbyterian University Hospital, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Surg Infect (Larchmt) 8:5-14. 2007
  2. pmc The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Exp Med 201:1135-43. 2005
  3. pmc Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2
    Yuehua Li
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Physiol Regul Integr Comp Physiol 297:R1670-80. 2009
  4. doi request reprint Histidine-tryptophan-ketoglutarate or celsior: which is more suitable for cold preservation for cardiac grafts from older donors?
    Sungsoo Lee
    Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Ann Thorac Surg 91:755-63. 2011
  5. pmc Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress
    Mallikarjuna R Metukuri
    Department of Surgery, Center for Biologic Imaging, University of Pittsburgh School of Medicine, W943 Biomedical Sciences Tower, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 296:G499-509. 2009
  6. ncbi request reprint Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway
    Ruiping Song
    Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh School of Medicine, 3459 5th Avenue, Pittsburgh, PA 15213, USA
    J Immunol 172:1220-6. 2004
  7. ncbi request reprint The role of initial trauma in the host's response to injury and hemorrhage: insights from a correlation of mathematical simulations and hepatic transcriptomic analysis
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Shock 26:592-600. 2006
  8. ncbi request reprint Toll-like receptor-4 signaling mediates hepatic injury and systemic inflammation in hemorrhagic shock
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Am Coll Surg 202:407-17. 2006
  9. doi request reprint Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both
    Atsunori Nakao
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittburgh, Pennsylvania 15213, USA
    J Heart Lung Transplant 29:544-53. 2010
  10. doi request reprint A novel method of preserving cardiac grafts using a hydrogen-rich water bath
    Kentaro Noda
    Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    J Heart Lung Transplant 32:241-50. 2013

Collaborators

Detail Information

Publications115 found, 100 shown here

  1. ncbi request reprint Making progress in an enlightened era: opportunities and obstacles
    Timothy R Billiar
    Presbyterian University Hospital, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Surg Infect (Larchmt) 8:5-14. 2007
  2. pmc The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Exp Med 201:1135-43. 2005
    ..Together, these results demonstrate that HMGB1 is an early mediator of injury and inflammation in liver I/R and implicates TLR4 as one of the receptors that is involved in the process...
  3. pmc Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2
    Yuehua Li
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Am J Physiol Regul Integr Comp Physiol 297:R1670-80. 2009
    ....
  4. doi request reprint Histidine-tryptophan-ketoglutarate or celsior: which is more suitable for cold preservation for cardiac grafts from older donors?
    Sungsoo Lee
    Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Ann Thorac Surg 91:755-63. 2011
    ....
  5. pmc Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress
    Mallikarjuna R Metukuri
    Department of Surgery, Center for Biologic Imaging, University of Pittsburgh School of Medicine, W943 Biomedical Sciences Tower, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 296:G499-509. 2009
    ..In turn, the upregulation of BNIP3 appears to be one mechanism of hepatocyte cell death and liver damage in these settings...
  6. ncbi request reprint Carbon monoxide inhibits T lymphocyte proliferation via caspase-dependent pathway
    Ruiping Song
    Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh School of Medicine, 3459 5th Avenue, Pittsburgh, PA 15213, USA
    J Immunol 172:1220-6. 2004
    ....
  7. ncbi request reprint The role of initial trauma in the host's response to injury and hemorrhage: insights from a correlation of mathematical simulations and hepatic transcriptomic analysis
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Shock 26:592-600. 2006
    ..Mathematical simulations and DNA microarrays, both systems biology tools, may provide valuable insight into the complex global physiological interactions that occur in response to trauma and hemorrhagic shock...
  8. ncbi request reprint Toll-like receptor-4 signaling mediates hepatic injury and systemic inflammation in hemorrhagic shock
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Am Coll Surg 202:407-17. 2006
    ..We sought to determine the role of lipolysaccharide through use of CD14-/- mice...
  9. doi request reprint Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both
    Atsunori Nakao
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittburgh, Pennsylvania 15213, USA
    J Heart Lung Transplant 29:544-53. 2010
    ..This study was designed to evaluate the effects of inhaled hydrogen or CO, or both, on cold ischemia/reperfusion (I/R) injury of the myocardium...
  10. doi request reprint A novel method of preserving cardiac grafts using a hydrogen-rich water bath
    Kentaro Noda
    Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    J Heart Lung Transplant 32:241-50. 2013
    ..Our objective in this study was to evaluate the efficacy of a novel cold storage device equipped with a hydrogen-rich water bath...
  11. pmc Mechanisms of Toll-like receptor 4 (TLR4)-mediated inflammation after cold ischemia/reperfusion in the heart
    David J Kaczorowski
    Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 87:1455-63. 2009
    ..High mobility group box 1 (HMGB1) is a putative endogenous activator of TLR4. Therefore, we also assessed the contribution of HMGB1 in cold I/R induced inflammation...
  12. doi request reprint Galantamine and carbon monoxide protect brain microvascular endothelial cells by heme oxygenase-1 induction
    Atsunori Nakao
    Department of Surgery, University of Pittsburgh Medical Center, E1551 BST, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Biochem Biophys Res Commun 367:674-9. 2008
    ..This pharmacological action of galantamine may, at least in part, account for the superior clinical efficacy of galantamine in vascular dementia and Alzheimer disease...
  13. ncbi request reprint Systemic inflammation and end organ damage following trauma involves functional TLR4 signaling in both bone marrow-derived cells and parenchymal cells
    Kevin P Mollen
    Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15217, USA
    J Leukoc Biol 83:80-8. 2008
    ..These data demonstrate that functional TLR4 is required in BM-derived cells and parenchymal cells for an optimal inflammatory response to trauma...
  14. pmc Hemorrhagic shock activates lung endothelial reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase via neutrophil NADPH oxidase
    Meng Xiang
    Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA
    Am J Respir Cell Mol Biol 44:333-40. 2011
    ..Oxidant signaling by neutrophil NADPH oxidase is an important determinant of activation of endothelial NADPH oxidase after HS...
  15. ncbi request reprint Systemic inflammation and remote organ injury following trauma require HMGB1
    Ryan M Levy
    Dept of Surgery, F 1200 PUH, Univ of Pittsburgh, 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1538-44. 2007
    ..Collectively, these data demonstrate a critical role for a TLR4-HMGB1 pathway in the initiation of systemic inflammation and end-organ injury following isolated peripheral tissue injury...
  16. pmc Preservation solution supplemented with biliverdin prevents lung cold ischaemia/reperfusion injury
    Ryujiro Sugimoto
    Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Eur J Cardiothorac Surg 42:1035-41. 2012
    ..We hypothesized that adding BV to the perfusate and cold storage solution could protect rat lung grafts from oxidative injuries via its antioxidant efficacies...
  17. ncbi request reprint Debunking the survival bias myth: characterization of mortality during the initial 24 hours for patients requiring massive transfusion
    Joshua B Brown
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma Acute Care Surg 73:358-64; discussion 364. 2012
    ..We sought to characterize mortality during the first 24 hours while controlling for time varying effects of transfusion to minimize survival bias...
  18. pmc In silico and in vivo approach to elucidate the inflammatory complexity of CD14-deficient mice
    Jose M Prince
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Mol Med 12:88-96. 2006
    ....
  19. ncbi request reprint Cutting edge: high-mobility group box 1 preconditioning protects against liver ischemia-reperfusion injury
    Kunihiko Izuishi
    Department of Surgery, University of Pittsburgh, PA 15213, USA
    J Immunol 176:7154-8. 2006
    ..Our studies demonstrate that in contrast to the role of HMGB1 as an early mediator of inflammation and organ damage in hepatic I/R, HMGB1 preconditioning can be protective...
  20. ncbi request reprint Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling
    Jie Fan
    Department of Surgery, School of Medicine, University of Pittsburgh, and Division of Pediatric Surgery, Children s Hospital of Pittsburgh, PA 15213, USA
    J Immunol 178:6573-80. 2007
    ..Thus, PMN NAD(P)H oxidase activation, induced by HS/R and as mediated by HMGB1/TLR4 signaling, is an important mechanism responsible for PMN-mediated inflammation and organ injury after hemorrhage...
  21. doi request reprint Superior myocardial preservation with HTK solution over Celsior in rat hearts with prolonged cold ischemia
    Sungsoo Lee
    The Heart, Lung and Esophageal Surgery Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Surgery 148:463-73. 2010
    ..The purpose of this study was to evaluate the protective effects of histidine-tryptophan-ketoglutarate (HTK) and Celsior (CEL) using a rat HTx model with prolonged cold storage...
  22. ncbi request reprint Carbon monoxide prevents multiple organ injury in a model of hemorrhagic shock and resuscitation
    Brian S Zuckerbraun
    Department of Surgery, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Shock 23:527-32. 2005
    ..The precise cellular mechanisms involved require further elucidation. CO may prove to be an adjunctive therapy that could be instituted rapidly and with ease as an out-of-hospital therapeutic modality for severe blood loss after trauma...
  23. doi request reprint A critical role for IFN regulatory factor 1 in NKT cell-mediated liver injury induced by alpha-galactosylceramide
    Zongxian Cao
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 185:2536-43. 2010
    ..Our findings revealed a novel mechanism of NKT cell-mediated liver injury in mice, which has implications in the development of human liver diseases...
  24. ncbi request reprint Hepatic ischemia/reperfusion injury involves functional TLR4 signaling in nonparenchymal cells
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 175:7661-8. 2005
    ..Together, these results demonstrate that TLR4 engagement on actively phagocytic nonparenchymal cells such as Kupffer cells is required for warm I/R-induced injury and inflammation in the liver...
  25. pmc Models of lower extremity damage in mice: time course of organ damage and immune response
    Christoph L Menzel
    Department of Orthopedic Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
    J Surg Res 166:e149-56. 2011
    ..This follow-up study tests the hypothesis that inflammatory responses are comparable between a novel trauma model ("pseudofracture", PFx) and a bilateral femur fracture (BFF) model...
  26. pmc Histones activate the NLRP3 inflammasome in Kupffer cells during sterile inflammatory liver injury
    Hai Huang
    Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    J Immunol 191:2665-79. 2013
    ..These novel findings illustrate a new mechanism by which extracellular histones and activation of NLRP3 inflammasome contribute to liver damage and the activation of innate immunity during sterile inflammation. ..
  27. pmc Complement factor 3 deficiency attenuates hemorrhagic shock-related hepatic injury and systemic inflammatory response syndrome
    Changchun Cai
    Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261, USA
    Am J Physiol Regul Integr Comp Physiol 299:R1175-82. 2010
    ..Our data indicate that complement activation contributes to inflammatory pathways and liver damage in HS/T. This suggests that targeting complement activation in the setting of severe injury could be useful...
  28. pmc Inducible protein-10, a potential driver of neurally controlled interleukin-10 and morbidity in human blunt trauma
    Akram M Zaaqoq
    1Department of Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA 2Department of Surgery, University of Pittsburgh, Pittsburgh, PA 3Center for Inflammation and Regenerative Modeling, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 4Department of Sports Medicine and Nutrition, University of Pittsburgh, Pittsburgh, PA 5Department of Rehabilitation Science and Technology, University of Pittsburgh, Pittsburgh, PA 6Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA
    Crit Care Med 42:1487-97. 2014
    ..We used in silico methods to discern dynamic inflammatory networks that could distinguish systemic inflammation in traumatic spinal cord injury from blunt trauma...
  29. ncbi request reprint Systemic inflammation and remote organ damage following bilateral femur fracture requires Toll-like receptor 4
    Ryan M Levy
    Department of Surgery, F 1200 PUH, University of Pittsburgh, 200 Lothrop St, Pittsburgh, PA 15217, USA
    Am J Physiol Regul Integr Comp Physiol 291:R970-6. 2006
    ..Application of these findings in an evolutionary context suggests that multicellular organisms have evolved to use the same pattern recognition receptor for surviving traumatic and infectious challenges...
  30. pmc Carbon monoxide decreases the level of iNOS protein and active dimer in IL-1beta-stimulated hepatocytes
    Hoe Suk Kim
    Department of Surgery, University of Pittsburgh School of Medicine, 200 Lothrop Street, Presbyterian Hospital F1200, Pittsburgh, PA 15213, USA
    Nitric Oxide 18:256-65. 2008
    ..Our results imply that CO exposure decreases NO production by suppressing dimer formation and increasing iNOS degradation through a process involving p38 activation...
  31. pmc Oral intake of hydrogen-rich water inhibits intimal hyperplasia in arterialized vein grafts in rats
    Qiang Sun
    Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Cardiovasc Res 94:144-53. 2012
    ..Hydrogen potently protects organs and cells from many insults via its anti-inflammatory and antioxidant properties. We investigated the efficacy of oral administration of hydrogen-rich water (HW) for prevention of intimal hyperplasia...
  32. pmc Goal-directed resuscitation in the prehospital setting: a propensity-adjusted analysis
    Joshua B Brown
    Department of Surgery, Division of General Surgery and Trauma, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma Acute Care Surg 74:1207-12; discussion 1212-4. 2013
    ..Optimal PH crystalloid resuscitation strategies remain unclear in blunt trauma as does the influence of PH hypotension. The objective was to characterize outcomes for PH crystalloid volume in patients with and without PH hypotension...
  33. ncbi request reprint Hypoxia activates c-Jun N-terminal kinase via Rac1-dependent reactive oxygen species production in hepatocytes
    Kevin P Mollen
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Shock 28:270-7. 2007
    ..Together, these data suggest that ROS generation during hypoxia in the liver directly leads to JNK activation in a Rac1-dependent process...
  34. doi request reprint Hydrogen-enriched preservation protects the isogeneic intestinal graft and amends recipient gastric function during transplantation
    Bettina M Buchholz
    Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
    Transplantation 92:985-92. 2011
    ..Inhaled hydrogen gas exerts antioxidant and anti-inflammatory effects in rat intestinal transplantation. Here, we investigated whether ex vivo donor organ treatment with dissolved hydrogen would prevent intestinal graft injury...
  35. ncbi request reprint Toll-like receptor 4 mediates the early inflammatory response after cold ischemia/reperfusion
    David J Kaczorowski
    Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 84:1279-87. 2007
    ..We tested the hypothesis that TLR4 mediates the early inflammatory response in the setting of cold I/R in a murine cardiac transplant model...
  36. pmc American College of Surgeons trauma center verification versus state designation: are Level II centers slipping through the cracks?
    Joshua B Brown
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma Acute Care Surg 75:44-9; discussion 49. 2013
    ..Single-center experience has shown that American College of Surgeons (ACS) trauma verification can improve outcomes. The current objective was to compare mortality between ACS-verified and state-designated centers in a national sample...
  37. pmc Interacting neuroendocrine and innate and acquired immune pathways regulate neutrophil mobilization from bone marrow following hemorrhagic shock
    Yujian Liu
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 182:572-80. 2009
    ..Blocking this novel signaling axis may present a novel therapeutic target for posttrauma inflammation...
  38. pmc Lipopolysaccharide clearance, bacterial clearance, and systemic inflammatory responses are regulated by cell type-specific functions of TLR4 during sepsis
    Meihong Deng
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 190:5152-60. 2013
    ....
  39. pmc Leukocyte-derived inducible nitric oxide synthase mediates murine postoperative ileus
    Andreas Türler
    Department of Medicine, Division of Gastroenterology, University of Pittsburgh, Pittsburgh, PA, USA
    Ann Surg 244:220-9. 2006
    ..To provide evidence that iNOS expression solely in leukocytes plays a role in postoperative ileus...
  40. doi request reprint Low-dose cisplatin administration in murine cecal ligation and puncture prevents the systemic release of HMGB1 and attenuates lethality
    Pinhua Pan
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Leukoc Biol 86:625-32. 2009
    ..Platinating agents in general and Cis specifically may be a novel approach to the treatment of sepsis...
  41. pmc Hemorrhagic shock activation of NLRP3 inflammasome in lung endothelial cells
    Meng Xiang
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 187:4809-17. 2011
    ..The present study explores a novel mechanism underlying HS activation of EC inflammasome and thus presents a potential therapeutic target for SIRS and ALI induced after HS...
  42. doi request reprint The role of fracture-associated soft tissue injury in the induction of systemic inflammation and remote organ dysfunction after bilateral femur fracture
    Philipp Kobbe
    Department of Orthopedic Surgery, University of Pittsburghm Kaufmann Medical Building, Pittsburgh, PA 15213, USA
    J Orthop Trauma 22:385-90. 2008
    ..We hypothesized that fracture-associated soft tissue injury plays a pivotal role in the induction of systemic inflammation and remote organ dysfunction after fracture...
  43. doi request reprint Body adipose content is independently associated with a higher risk of organ failure and nosocomial infection in the nonobese patient postinjury
    Rebecca D Edmonds
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma 70:292-8. 2011
    ..We sought to avoid the confounding effects attributable to obesity and determine the association of BMI and outcomes following injury in nonobese patients...
  44. pmc DNA attenuates enterocyte Toll-like receptor 4-mediated intestinal mucosal injury after remote trauma
    Chhinder Sodhi
    Div of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, PA 15224, USA
    Am J Physiol Gastrointest Liver Physiol 300:G862-73. 2011
    ....
  45. pmc Endothelial TLR4 activation impairs intestinal microcirculatory perfusion in necrotizing enterocolitis via eNOS-NO-nitrite signaling
    Ibrahim Yazji
    Division of Pediatric Surgery, Children s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA
    Proc Natl Acad Sci U S A 110:9451-6. 2013
    ....
  46. ncbi request reprint Increasing numbers of hepatic dendritic cells promote HMGB1-mediated ischemia-reperfusion injury
    Allan Tsung
    Department of Surgery, University of Pittsburgh School of Medicine, 200 Lothrop Street, Presbyterian Hospital F1200, Pittsburgh, PA 15213, USA
    J Leukoc Biol 81:119-28. 2007
    ..It thus appears that functional TLR4 on DC is required for I/R-induced injury. Furthermore, HMGB1 may direct the inflammatory responses mediated by DC, at least in part, by enhancing TLR4 expression and reactivity to it and other DAMPs...
  47. doi request reprint Orally administered nitrite attenuates cardiac allograft rejection in rats
    Jianghua Zhan
    Department of Surgery, Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA
    Surgery 146:155-65. 2009
    ..Recent studies have shown that nitrite serves as an endogenous reservoir of nitric oxide (NO), particularly in the presence of hypoxia and ischemia. We hypothesized that exogenous nitrite supplementation would protect cardiac allografts...
  48. ncbi request reprint Induced nitric oxide promotes intestinal inflammation following hemorrhagic shock
    Christian Hierholzer
    Department of Surgery, University of Pittsburgh Medical Center, F1264 200 Lothrop St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 286:G225-33. 2004
    ....
  49. pmc Endotoxin uptake in mouse liver is blocked by endotoxin pretreatment through a suppressor of cytokine signaling-1-dependent mechanism
    Melanie J Scott
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Hepatology 49:1695-708. 2009
    ..In the same samples, ubiquitinated TIRAP was detected after 4 hours and up to 8 hours after LPS stimulation, but not at later times...
  50. ncbi request reprint Nitric oxide and ionizing radiation synergistically promote apoptosis and growth inhibition of cancer by activating p53
    Tracy Cook
    Department of Surgery and Center for Biologic Imaging, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15232, USA
    Cancer Res 64:8015-21. 2004
    ..Furthermore, the transfer of both p53 and iNOS genes into tumor cells lacking functional p53 enhanced their radioresponsiveness more than transfer of either gene alone...
  51. pmc Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes
    Melanie J Scott
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 283:29433-46. 2008
    ..However, TLR4/MD2 remain essential components at the cell surface as part of the LPS receptor complex. We therefore suggest novel roles for TLR4/MD2, CD11b/CD18, TIRAP, and p38 MAPK in LPS uptake by hepatocytes...
  52. pmc Caspase 1 activation is protective against hepatocyte cell death by up-regulating beclin 1 protein and mitochondrial autophagy in the setting of redox stress
    Qian Sun
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 288:15947-58. 2013
    ....
  53. pmc Preactivation of NKT cells with alpha-GalCer protects against hepatic ischemia-reperfusion injury in mouse by a mechanism involving IL-13 and adenosine A2A receptor
    Zongxian Cao
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Am J Physiol Gastrointest Liver Physiol 297:G249-58. 2009
    ..These findings provide the first evidence that hepatic preconditioning by preactivation of NKT cells with alpha-GalCer protects the liver from IR injury via an IL-13 and adenosine A2AR-dependent mechanism...
  54. doi request reprint Interferon regulatory factor 1 mediates acetylation and release of high mobility group box 1 from hepatocytes during murine liver ischemia-reperfusion injury
    Rajeev Dhupar
    Department of Surgery, and Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Shock 35:293-301. 2011
    ..Together, these findings suggest that I/R-induced release of acetylated HMGB1 is a process that is dependent on TLR4-mediated upregulation of IRF-1...
  55. pmc Crystalloid to packed red blood cell transfusion ratio in the massively transfused patient: when a little goes a long way
    Matthew D Neal
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma Acute Care Surg 72:892-8. 2012
    ..We hypothesized that an increased crystalloid:PRBC (C:PRBC) ratio would be associated with increased morbidity and poor outcome after MT...
  56. ncbi request reprint The role of RAGE in the pathogenesis of intestinal barrier dysfunction after hemorrhagic shock
    Kathleen G Raman
    Univ of Pittsburgh School of Medicine, 616 Scaife Hall, 3550 Terrace St, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 291:G556-65. 2006
    ..Circulating IL-10 levels were higher in rage(-/-) compared with rage(+/+) mice. These results suggest that activation of RAGE-dependent signaling is a key factor leading to gut mucosal barrier dysfunction after HS/R...
  57. pmc Preconditioning with high mobility group box 1 (HMGB1) induces lipopolysaccharide (LPS) tolerance
    RAJESH K ANEJA
    Department of Critical Care Medicine and Pediatrics, Children s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Leukoc Biol 84:1326-34. 2008
    ..Taken together, these results suggest that extracellular HMGB1 induces LPS tolerance, and the RAGE receptor is required for this induction...
  58. doi request reprint Local exposure of bone components to injured soft tissue induces Toll-like receptor 4-dependent systemic inflammation with acute lung injury
    Philipp Kobbe
    Department of Orthopaedic Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Shock 30:686-91. 2008
    ..These findings suggest that bone components contribute to systemic inflammation and acute lung injury after long bone fractures via TLR-4 signaling and support the notion of a central role for TLR-4 in sensing tissue damage...
  59. pmc Characterization of acute coagulopathy and sexual dimorphism after injury: females and coagulopathy just do not mix
    Joshua B Brown
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    J Trauma Acute Care Surg 73:1395-400. 2012
    ..It remains unclear whether presence of ATC alters sex-based outcomes after injury. This study's objective was to characterize the sex dimorphism after severe injury in the presence and absence of ATC...
  60. pmc Cellular-specific role of toll-like receptor 4 in hepatic ischemia-reperfusion injury in mice
    Gary W Nace
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA
    Hepatology 58:374-87. 2013
    ....
  61. pmc Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts
    Atsunori Nakao
    Department of Surgery, University of Pittsburgh Medical Center, E1551, Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Cardiovasc Res 89:457-63. 2011
    ..We investigated the feasibility and efficacy of the ex vivo pre-treatment of vein grafts with soluble carbon monoxide (CO) in the inhibition of IH...
  62. pmc Hemorrhagic shock augments Nlrp3 inflammasome activation in the lung through impaired pyrin induction
    Peng Xu
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 190:5247-55. 2013
    ..This study demonstrates a novel mechanism by which HS suppresses negative-feedback regulation of Nlrp3 inflammasome to enhance IL-1β secretion in response to subsequent LPS challenge and so primes for inflammation...
  63. pmc Pivotal advance: The pattern recognition receptor ligands lipopolysaccharide and polyinosine-polycytidylic acid stimulate factor B synthesis by the macrophage through distinct but overlapping mechanisms
    David J Kaczorowski
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Leukoc Biol 88:609-18. 2010
    ..Understanding the mechanisms of PRR-mediated complement production may lead to strategies aimed at preventing tissue damage in diverse settings, including sepsis, trauma, and ischemia/reperfusion...
  64. pmc Role of toll-like receptors in changes in gene expression and NF-kappa B activation in mouse hepatocytes stimulated with lipopolysaccharide
    Shubing Liu
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Infect Immun 70:3433-42. 2002
    ..Thus, hepatocytes respond to nanogram concentrations of LPS through a TLR4 response pathway...
  65. ncbi request reprint Peroxisomal localization of inducible nitric oxide synthase in hepatocytes
    Donna Beer Stolz
    Department of Cell Biology and Physiology, University of Pittsburgh Medical School, Pittsburgh, PA 12561, USA
    Hepatology 36:81-93. 2002
    ..In conclusion, these studies establish the peroxisome as a site of iNOS localization in hepatocytes and show a relationship between iNOS up-regulation and decreased expression of catalase...
  66. ncbi request reprint Regulation of tissue factor expression in smooth muscle cells with nitric oxide
    Melina R Kibbe
    Department of Surgery, A 1011, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Vasc Surg 37:650-9. 2003
    ..This study was undertaken to determine the effect of nitric oxide (NO) on tissue factor (TF) expression in vascular smooth muscle cells...
  67. doi request reprint Changes in FADD levels, distribution, and phosphorylation in TNFalpha-induced apoptosis in hepatocytes is caspase-3, caspase-8 and BID dependent
    Xiaoying Zhang
    Department of Surgery, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15213, USA
    Apoptosis 13:983-92. 2008
    ..Changes in FADD levels and distribution may represent a novel feed-forward mechanism to propagate apoptosis signaling in hepatocytes...
  68. pmc Nitric oxide and thioredoxin type 1 modulate the activity of caspase 8 in HepG2 cells
    Rajib Sengupta
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Biochem Biophys Res Commun 391:1127-30. 2010
    ..The data obtained suggest that extrinsic apoptosis can be subjected to redox regulation before induction of proteolytic damage by caspase 3...
  69. ncbi request reprint Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway
    Hoe Suk Kim
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Biochem Biophys Res Commun 344:1172-8. 2006
    ..These data indicate that CO interferes with apoptotic signaling at a proximal step...
  70. ncbi request reprint A DNA microarray study of nitric oxide-induced genes in mouse hepatocytes: implications for hepatic heme oxygenase-1 expression in ischemia/reperfusion
    Ruben Zamora
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Nitric Oxide 7:165-86. 2002
    ....
  71. ncbi request reprint The acute inflammatory response in diverse shock states
    Carson C Chow
    Department of Mathematics, University of Pittsburgh, Pittsburgh, Pensylvania 15261, USA
    Shock 24:74-84. 2005
    ....
  72. ncbi request reprint Thioredoxin catalyzes the denitrosation of low-molecular mass and protein S-nitrosothiols
    Rajib Sengupta
    Department of Surgery, The University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Biochemistry 46:8472-83. 2007
    ..The data obtained are discussed within the general reaction mechanisms encompassing the cellular homeostasis of S-nitrosothiols...
  73. ncbi request reprint Autologous nitric oxide protects mouse and human keratinocytes from ultraviolet B radiation-induced apoptosis
    Richard Weller
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Am J Physiol Cell Physiol 284:C1140-8. 2003
    ..These results demonstrate an antiapoptotic role for NO in keratinocytes, mediated by cGMP, and indicate an antiapoptotic role for both eNOS and iNOS in skin damage induced by UVB...
  74. ncbi request reprint Carbon monoxide suppresses arteriosclerotic lesions associated with chronic graft rejection and with balloon injury
    Leo E Otterbein
    Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh School of Medicine, Montefiore University Hospital, Pittsburgh, Pennsylvania, USA
    Nat Med 9:183-90. 2003
    ..These findings demonstrate a protective role for CO in vascular injury and support its use as a therapeutic agent...
  75. pmc Calcium/calmodulin-dependent protein kinase (CaMK) IV mediates nucleocytoplasmic shuttling and release of HMGB1 during lipopolysaccharide stimulation of macrophages
    Xianghong Zhang
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 181:5015-23. 2008
    ..Collectively, our results demonstrate that CaMKIV promotes the nucleocytoplasmic shuttling of HMGB1 and suggest that the process may be mediated through CaMKIV-dependent serine phosphorylation of HMGB1...
  76. ncbi request reprint The role of hepatic type 1 plasminogen activator inhibitor (PAI-1) during murine hemorrhagic shock
    Claudio E Lagoa
    Department of Surgery, University of Pittsburgh, S411 B South BST, Pittsburgh, PA 15261, USA
    Hepatology 42:390-9. 2005
    ..In conclusion, PAI-1 protein is a negative effector of hepatic damage after HS-R through its influence on classic regulators of hepatic growth, as opposed to its role in fibrinolysis...
  77. ncbi request reprint Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation
    Jie Fan
    Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15240, USA
    Am J Physiol Lung Cell Mol Physiol 290:L738-L746. 2006
    ..Thus TLR2 expression in AMphi, signaled by TLR4 and regulated by shock-activated PMN, is an important positive-feedback mechanism responsible for shock-primed PMN infiltration into the lung after primary PMN sequestration...
  78. doi request reprint Carbon monoxide activates NF-kappaB via ROS generation and Akt pathways to protect against cell death of hepatocytes
    Hoe Suk Kim
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Am J Physiol Gastrointest Liver Physiol 295:G146-G152. 2008
    ..This pathway may prove to be important in maintenance of hepatic function in both physiological and pathophysiological conditions...
  79. pmc HMGB1 release induced by liver ischemia involves Toll-like receptor 4 dependent reactive oxygen species production and calcium-mediated signaling
    Allan Tsung
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Exp Med 204:2913-23. 2007
    ..Collectively, these results demonstrate that hypoxia-induced HMGB1 release by hepatocytes is an active, regulated process that occurs through a mechanism promoted by TLR4-dependent ROS production and downstream CaMK-mediated signaling...
  80. pmc Hydrogen inhalation ameliorates ventilator-induced lung injury
    Chien Sheng Huang
    Department of Cardiothoracic Surgery, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Crit Care 14:R234. 2010
    ..Inhaled hydrogen can act as an antioxidant and may be useful as a novel therapeutic gas. We hypothesized that, owing to its antioxidant and anti-inflammatory properties, inhaled hydrogen therapy could ameliorate VILI...
  81. pmc Incidental radiographic findings after injury: dedicated attention results in improved capture, documentation, and management
    Jason L Sperry
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Surgery 148:618-24. 2010
    ..We hypothesized a dedicated incidental finding coordinator would increase incidental finding capture and promote notification, follow-up, and documentation of incidental finding events...
  82. pmc Hepatocytes express functional NOD1 and NOD2 receptors: a role for NOD1 in hepatocyte CC and CXC chemokine production
    Melanie J Scott
    Department of Surgery, University of Pittsburgh School of Medicine, NW 607 MUH, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    J Hepatol 53:693-701. 2010
    ..Both NOD1 and NOD2 have been associated with many inflammatory diseases, although their role in liver inflammation and infection has not been well studied...
  83. pmc An adequately robust early TNF-alpha response is a hallmark of survival following trauma/hemorrhage
    Rajaie Namas
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS ONE 4:e8406. 2009
    ..Trauma/hemorrhagic shock (T/HS) results in cytokine-mediated acute inflammation that is generally considered detrimental...
  84. doi request reprint Fresh frozen plasma is independently associated with a higher risk of multiple organ failure and acute respiratory distress syndrome
    Gregory A Watson
    Division of General Surgery and Trauma, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA
    J Trauma 67:221-7; discussion 228-30. 2009
    ..We hypothesized that plasma-rich transfusion components would be independently associated with a lower risk of mortality but result in a greater risk of morbid complications...
  85. ncbi request reprint Nitric oxide-mediated inhibition of caspase-dependent T lymphocyte proliferation
    Raja S Mahidhara
    Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    J Leukoc Biol 74:403-11. 2003
    ..Previous studies demonstrated inhibition of apoptosis through S-nitrosylation of caspases; the present studies extend this effect to inhibition of caspase-dependent T cell proliferation...
  86. ncbi request reprint Role of nitric oxide in liver injury
    Tracy Chen
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Curr Mol Med 3:519-26. 2003
    ..The purpose of this paper is to review the current understanding of the role of NO in liver injury...
  87. ncbi request reprint Blood soluble drag-reducing polymers prevent lethality from hemorrhagic shock in acute animal experiments
    Marina V Kameneva
    McGowan Institute for Regenerative Medicine, Department of Surgery, University of Pittsburgh, 3025 East Carson Street, Pittsburgh, PA 15203, USA
    Biorheology 41:53-64. 2004
    ..Our findings suggest that the drag-reducing polymers warrant further investigation as a potential clinical treatment for hemorrhagic shock and possibly other microcirculatory disorders...
  88. ncbi request reprint The Department of Surgery at the School of Medicine at the University Of Pittsburgh, Pittsburgh, Pa
    Timothy R Billiar
    Department of Surgery, Presbyterian University Hospital, Pittsburgh, PA 15213, USA
    Arch Surg 139:466-8. 2004
  89. ncbi request reprint Inflammatory modulation of hepatocyte apoptosis by nitric oxide: in vivo, in vitro, and in silico studies
    Yoram Vodovotz
    Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Curr Mol Med 4:753-62. 2004
    ..Our studies suggest that modulation of iron, oxygen, and superoxide may dictate whether NO* is hepatoprotective or hepatotoxic...
  90. ncbi request reprint Thioredoxin and lipoic acid catalyze the denitrosation of low molecular weight and protein S-nitrosothiols
    Detcho A Stoyanovsky
    Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Am Chem Soc 127:15815-23. 2005
    ..We conclude that both thioredoxin and dihydrolipoic acid may be involved in the regulation of cellular S-nitrosothiols...
  91. ncbi request reprint Emerging paradigm: toll-like receptor 4-sentinel for the detection of tissue damage
    Kevin P Mollen
    Department of Surgery, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    Shock 26:430-7. 2006
    ..This review will detail the observations implicating a TLR family member, TLR4, as a key component of the initial injury response...
  92. doi request reprint Patterns of cytokine release and evolution of remote organ dysfunction after bilateral femur fracture
    Philipp Kobbe
    Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Shock 30:43-7. 2008
    ....
  93. ncbi request reprint Carbon monoxide signals via inhibition of cytochrome c oxidase and generation of mitochondrial reactive oxygen species
    Brian S Zuckerbraun
    Department of Surgery, University of Pittsburgh School of Medicine, NW653 MUH, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    FASEB J 21:1099-106. 2007
    ....
  94. doi request reprint Male gender is associated with excessive IL-6 expression following severe injury
    Jason L Sperry
    Division of Trauma and General Surgery, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    J Trauma 64:572-8; discussion 578-9. 2008
    ....
  95. doi request reprint An FFP:PRBC transfusion ratio >/=1:1.5 is associated with a lower risk of mortality after massive transfusion
    Jason L Sperry
    Division of General Surgery and Trauma, Departments of Surgery, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    J Trauma 65:986-93. 2008
    ....
  96. pmc Anti-HMGB1 neutralizing antibody ameliorates gut barrier dysfunction and improves survival after hemorrhagic shock
    Runkuan Yang
    Department of Critical Care Medicine, University of Pittsburgh School of Medicine, PA 15621, USA
    Mol Med 12:105-14. 2006
    ..These data support the notion that HMGB1 is a mediator of HS/R-induced gut barrier dysfunction and suggest that anti-HMGB1 antibodies warrant further evaluation as a therapeutic to ameliorate the morbidity of HS/R in trauma patients...
  97. ncbi request reprint Nitric oxide prevents 6-hydroxydopamine-induced apoptosis in PC12 cells through cGMP-dependent PI3 kinase/Akt activation
    Kwon Soo Ha
    Vascular System Research Center and Department of Molecular and Cellular Biochemistry, Kangwon National University, School of Medicine, Chunchon, Kangwon Do, Korea
    FASEB J 17:1036-47. 2003
    ..Our data suggest that the NO/cGMP pathway suppresses 6-OHDA-induced PC12 cell apoptosis by suppressing the mitochondrial apoptosis signal via PKG/PI3K/Akt-dependent Bad phosphorylation...
  98. pmc Intra-abdominal activation of a local inflammatory response within the human muscularis externa during laparotomy
    Jorg C Kalff
    Department of Medicine, University of Pittsburgh, Pennsylvania 15261, USA
    Ann Surg 237:301-15. 2003
    ..To investigate the initiation of a complex inflammatory response within the human intestinal muscularis intraoperatively so as to determine the clinical applicability of the inflammatory hypothesis of postoperative ileus...
  99. ncbi request reprint Ultraviolet irradiation increases FADD protein in apoptotic human keratinocytes
    Peter K M Kim
    Department of Surgery, University of Pittsburgh Medical Center, NW607 MUH, Pittsburgh, PA 15213, USA
    Biochem Biophys Res Commun 302:290-5. 2003
    ..Inhibition of FADD protein by adenoviral expression of anti-sense FADD reduced keratinocyte apoptosis. Regulation of FADD expression by UV may serve to enhance death receptor-mediated keratinocyte death...
  100. ncbi request reprint Nitric oxide (NO) pretreatment increases cytokine-induced NO production in cultured rat hepatocytes by suppressing GTP cyclohydrolase I feedback inhibitory protein level and promoting inducible NO synthase dimerization
    Joon Hong Park
    Vascular System Research Center and Department of Molecular and Cellular Biochemistry, Kangwon National University, School of Medicine, Chunchon, Kangwon Do 200 701, Korea
    J Biol Chem 277:47073-9. 2002
    ..These results suggest that SNAP pretreatment increased NO production from iNOS by elevating cellular BH(4) levels and promoting iNOS subunit dimerization through the suppression of GFRP levels and subsequent activation of GTPCHI...
  101. ncbi request reprint Signaling and function of caspase and c-jun N-terminal kinase in cisplatin-induced apoptosis
    Myoung Sook Koo
    Vascular System Research Center, Kangwon National University, Chunchon, Korea
    Mol Cells 13:194-201. 2002
    ..These data suggest that the cisplatin-induced apoptotic signal is initiated by the caspase-8-independent cytochrome c release, and the JNK activation protects cells from cisplatin-induced apoptosis via the metallothionein expression...