MARK WEWERS

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. pmc Monocyte derived microvesicles deliver a cell death message via encapsulated caspase-1
    Anasuya Sarkar
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 4:e7140. 2009
  2. pmc A novel role for IkappaBzeta in the regulation of IFNgamma production
    RAQUEL M RAICES
    The Ohio State University, Davis Heart and Lung Research Institute, Columbus, Ohio, USA
    PLoS ONE 4:e6776. 2009
  3. pmc Receptor interacting protein-2 plays a critical role in human lung epithelial cells survival in response to Fas-induced cell-death
    Mohd Akhlakur Rahman
    Dorothy M Davis Heart and Lung Research Institute, Department of Internal Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Wexner Medical Center, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 9:e92731. 2014
  4. pmc Mycoplasma suppression of THP-1 Cell TLR responses is corrected with antibiotics
    Ekaterina Zakharova
    Division of Pulmonary Allergy Critical Care and Sleep Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 5:e9900. 2010
  5. ncbi request reprint Lung CD4 lymphocytes predict survival in asymptomatic HIV infection
    Mark D Wewers
    Pulmonary and Critical Care Division, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Chest 128:2262-7. 2005
  6. pmc P2X(7) receptor and macrophage function
    Mark D Wewers
    Davis Heart and Lung Research Institute, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Ohio State University, Columbus, OH, USA
    Purinergic Signal 5:189-95. 2009
  7. ncbi request reprint Cigarette smoking in HIV infection induces a suppressive inflammatory environment in the lung
    M D Wewers
    Department of Internal Medicine, College of Medicine and Public Health, Department of Statistics College of Mathematics, Physical Sciences, Ohio State University, Columbus, Ohio, USA
    Am J Respir Crit Care Med 158:1543-9. 1998
  8. ncbi request reprint Endotoxin-stimulated monocytes release multiple forms of IL-1 beta, including a proIL-1 beta form whose detection is affected by export
    M D Wewers
    Department of Pulmonary and Critical Care Medicine, Ohio State University, Columbus 43210, USA
    J Immunol 162:4858-63. 1999
  9. pmc IL-1beta: an endosomal exit
    Mark D Wewers
    Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 101:10241-2. 2004
  10. ncbi request reprint Pyrin levels in human monocytes and monocyte-derived macrophages regulate IL-1beta processing and release
    Sudarshan Seshadri
    Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    J Immunol 179:1274-81. 2007

Research Grants

Collaborators

Detail Information

Publications41

  1. pmc Monocyte derived microvesicles deliver a cell death message via encapsulated caspase-1
    Anasuya Sarkar
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 4:e7140. 2009
    ..This transcellular apoptosis induction pathway describes a novel pathway for inflammation induced programmed cell death...
  2. pmc A novel role for IkappaBzeta in the regulation of IFNgamma production
    RAQUEL M RAICES
    The Ohio State University, Davis Heart and Lung Research Institute, Columbus, Ohio, USA
    PLoS ONE 4:e6776. 2009
    ..Moreover, silencing of IkappaBzeta expression led to a specific decrease in IFNgamma production. Overall, our data suggests that IkappaBzeta positively regulates NFkappaB-mediated IFNgamma production in KG-1 cells...
  3. pmc Receptor interacting protein-2 plays a critical role in human lung epithelial cells survival in response to Fas-induced cell-death
    Mohd Akhlakur Rahman
    Dorothy M Davis Heart and Lung Research Institute, Department of Internal Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Wexner Medical Center, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 9:e92731. 2014
    ..Thus RIP2's CARD tyrosine phosphorylation may represent a new therapeutic target to promote the survival of human lung epithelial cells in disorders that lead to acute lung injury and ARDS. ..
  4. pmc Mycoplasma suppression of THP-1 Cell TLR responses is corrected with antibiotics
    Ekaterina Zakharova
    Division of Pulmonary Allergy Critical Care and Sleep Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 5:e9900. 2010
    ..Thus, routine screening of cell lines for mycoplasma is important for the maintenance of reliable experimental data and contaminated cell lines can be restored to their baseline function with antibiotic clearance of mycoplasma...
  5. ncbi request reprint Lung CD4 lymphocytes predict survival in asymptomatic HIV infection
    Mark D Wewers
    Pulmonary and Critical Care Division, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Chest 128:2262-7. 2005
    ..Plasma viral load and blood CD4 counts are accepted indicators of severity of illness in patients with HIV-1. Lung CD4 counts have not been evaluated in asymptomatic HIV-1 patients as indicators of disease severity...
  6. pmc P2X(7) receptor and macrophage function
    Mark D Wewers
    Davis Heart and Lung Research Institute, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Ohio State University, Columbus, OH, USA
    Purinergic Signal 5:189-95. 2009
    ..Therapeutic approaches to inflammation and sepsis will certainly be enhanced by an increased understanding of how purinergic receptors modulate the inflammasomes...
  7. ncbi request reprint Cigarette smoking in HIV infection induces a suppressive inflammatory environment in the lung
    M D Wewers
    Department of Internal Medicine, College of Medicine and Public Health, Department of Statistics College of Mathematics, Physical Sciences, Ohio State University, Columbus, Ohio, USA
    Am J Respir Crit Care Med 158:1543-9. 1998
    ..These observations show that cigarette smoking is associated with suppression in localized lung defenses, and suggest that smoking cessation may have a positive impact on lung defenses in HIV-infected smokers...
  8. ncbi request reprint Endotoxin-stimulated monocytes release multiple forms of IL-1 beta, including a proIL-1 beta form whose detection is affected by export
    M D Wewers
    Department of Pulmonary and Critical Care Medicine, Ohio State University, Columbus 43210, USA
    J Immunol 162:4858-63. 1999
    ..These findings imply that IL-1 beta is exported from monocytes as 3-, 17-, 28-, and 31-kDa forms and that the released 31-kDa form differs from cytosolic proIL-1 beta...
  9. pmc IL-1beta: an endosomal exit
    Mark D Wewers
    Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 101:10241-2. 2004
  10. ncbi request reprint Pyrin levels in human monocytes and monocyte-derived macrophages regulate IL-1beta processing and release
    Sudarshan Seshadri
    Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    J Immunol 179:1274-81. 2007
    ..In conclusion, in the context of endotoxin-induced activation of mononuclear phagocytes, pyrin augments IL-1beta processing and release...
  11. ncbi request reprint Janus kinase 3 down-regulates lipopolysaccharide-induced IL-1 beta-converting enzyme activation by autocrine IL-10
    Hee Jung Kim
    Division of Pulmonary and Critical Care Medicine, and Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    J Immunol 172:4948-55. 2004
    ..These findings support a model in which JAK3 signaling enhances IL-10 production leading to down-regulation of ICE activation and suppression of IL-1 beta processing and release...
  12. ncbi request reprint Abnormal permeability of inner and outer mitochondrial membranes contributes independently to mitochondrial dysfunction in the liver during acute endotoxemia
    Elliott D Crouser
    Division of Pulmonary and Critical Care Medicine, The Ohio State University Medical Center, Columbus, OH, USA
    Crit Care Med 32:478-88. 2004
    ..This study was designed to determine the role played by the mitochondrial permeability transition in the pathogenesis of mitochondrial damage and dysfunction in a representative systemic organ during the acute phase of endotoxemia...
  13. ncbi request reprint Monocyte mRNA phenotype and adverse outcomes from pediatric multiple organ dysfunction syndrome
    Mark W Hall
    Department of Pediatrics, Davis Heart and Lung Research Institute, The Ohio State University College of Medicine, Columbus, Ohio 43210, USA
    Pediatr Res 62:597-603. 2007
    ..02). These data suggest that adverse outcomes from pediatric MODS are associated with an anti-inflammatory monocyte mRNA phenotype. Future studies are warranted to explore mechanisms of immunodepression in pediatric critical illness...
  14. ncbi request reprint Regulation of monocyte apoptosis by the protein kinase Cdelta-dependent phosphorylation of caspase-3
    Oliver H Voss
    Heart and Lung Research Institute and Division of Pulmonary and Critical Care, Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 280:17371-9. 2005
    ..Our results provided evidence that the PKCdelta-dependent phosphorylation of caspase-3 provided a novel pro-apoptotic mechanism involved in the regulation of monocyte life span...
  15. ncbi request reprint Opposing effect by cytokines on Fas-mediated apoptosis in A549 lung epithelial cells
    Kristin R Coulter
    Department of Pharmacy, Ohio State University College of Pharmacy, and Department of Internal Medicine, Ohio State University College of Medicine, Columbus, Ohio 43210, USA
    Am J Respir Cell Mol Biol 26:58-66. 2002
    ..Finally, our investigation demonstrates that the differential impact of IL-1beta and IFN-gamma on Fas-mediated apoptosis are in part dependent on modulation of the PI 3-K/Akt survival pathway...
  16. pmc Pyrin critical to macrophage IL-1beta response to Francisella challenge
    Mikhail A Gavrilin
    Division of Pulmonary Allergy Critical Care and Sleep Medicine, Davis Heart and Lung Research Institute, Ohio State University, Columbus, OH 43210, USA
    J Immunol 182:7982-9. 2009
    ..novicida. In summary, intracellular pyrin levels positively regulate MDM IL-1beta responsiveness to Francisella challenge...
  17. ncbi request reprint Cyclosporin A ameliorates mitochondrial ultrastructural injury in the ileum during acute endotoxemia
    Elliott D Crouser
    Division of Pulmonary and Critical Care Medicine, The Ohio State University Medical Center, 201F Davis Heart and Lung Research Institute, 473 West Twelfth Avenue, Columbus, OH 43210 1252, USA
    Crit Care Med 30:2722-8. 2002
    ..This study was designed to determine the role, if any, of the mitochondrial permeability transition in the pathogenesis of mitochondrial injury in a representative systemic organ during the acute phase of endotoxemia...
  18. ncbi request reprint Interleukin-4-induced apoptosis entails caspase activation and suppression of extracellular signal-regulated kinase phosphorylation
    Andrea I Doseff
    The Dorothy M Davis Heart and Lung Research Institute, Division of Pulmonary, Ohio State University, Columbus 43210, USA
    Am J Respir Cell Mol Biol 29:367-74. 2003
    ..Together, these findings suggest a molecular mechanism by which the anti-inflammatory cytokine IL-4 modulates the life span of monocytes at least in part by an extracellular signal-regulated kinase-dependent pathway...
  19. pmc Sepsis: links between pathogen sensing and organ damage
    Elliott Crouser
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Curr Pharm Des 14:1840-52. 2008
    ....
  20. ncbi request reprint Apigenin blocks lipopolysaccharide-induced lethality in vivo and proinflammatory cytokines expression by inactivating NF-kappaB through the suppression of p65 phosphorylation
    Courtney Nicholas
    Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio 43210, USA
    J Immunol 179:7121-7. 2007
    ..Collectively, these findings suggest a molecular mechanism by which apigenin suppresses inflammation and modulates the immune response in vivo...
  21. ncbi request reprint Regulated expression and inhibitory function of Fcgamma RIIb in human monocytic cells
    Susheela Tridandapani
    Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:5082-9. 2002
    ..We, therefore, propose that FcgammaRIIb serves to inhibit FcgammaRI/IIa-mediated macrophage activation using SHIP as its effector...
  22. ncbi request reprint A novel P2X7 receptor activator, the human cathelicidin-derived peptide LL37, induces IL-1 beta processing and release
    Andreas Elssner
    The Dorothy M Davis Heart and Lung Research Institute and Pulmonary and Critical Care Division, Ohio State University, Columbus, OH 43210, USA
    J Immunol 172:4987-94. 2004
    ..We conclude that endogenous LL37 may promote IL-1 beta processing and release via direct activation of P2X(7) receptors...
  23. ncbi request reprint The acute respiratory distress syndrome: a role for transforming growth factor-beta 1
    Ruairi J Fahy
    Division of Pulmonary and Critical Care Medicine, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 1252, USA
    Am J Respir Cell Mol Biol 28:499-503. 2003
    ..These data may lead to new therapeutic approaches in ARDS through more targeted inhibition of fibrosis...
  24. pmc Internalization and phagosome escape required for Francisella to induce human monocyte IL-1beta processing and release
    Mikhail A Gavrilin
    Davis Heart and Lung Research Institute, Department of Molecular Virology and Immunology and Medical Genetics, and Center for Microbial Interface Biology, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 103:141-6. 2006
    ..These findings demonstrate that Francisella organisms efficiently induce IL-1beta processing and release in fresh monocytes by means of a sensing system that requires the uptake of live bacteria capable of phagosome escape...
  25. ncbi request reprint Regional differences in emphysema scores and BAL glutathione levels in HIV-infected individuals
    Philip T Diaz
    Heart Lung Research Institute, Columbus, OH 43210, USA
    Chest 126:1439-42. 2004
    ..We sought to determine the regional distribution of emphysema and regional distribution of glutathione (GSH) concentrations among HIV-seropositive subjects with emphysema...
  26. pmc Caspase-7 activation by the Nlrc4/Ipaf inflammasome restricts Legionella pneumophila infection
    Anwari Akhter
    Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Center for Microbial Interface Biology and the Department of Internal Medicine, Ohio State University, Columbus, OH, USA
    PLoS Pathog 5:e1000361. 2009
    ..These results reveal a new mechanism for caspase-7 activation downstream of the Nlrc4 inflammasome and present a novel biological role for caspase-7 in host defense against an intracellular bacterium...
  27. pmc Inflammasome mRNA expression in human monocytes during early septic shock
    Ruairi J Fahy
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, The Ohio State University Medical Center, Columbus, Ohio, USA
    Am J Respir Crit Care Med 177:983-8. 2008
    ..Monocytes are central to the initiation of the inflammatory response in sepsis, with caspase-1 activation playing a key role. Monocyte deactivation during sepsis has been linked to poor outcomes...
  28. doi request reprint MAIL regulates human monocyte IL-6 production
    Sudarshan Seshadri
    Davis Heart and Lung Research Institute and Pulmonary, Allergy, Critical Care and Sleep Medicine Division, The Ohio State University, Columbus, OH 43210, USA
    J Immunol 183:5358-68. 2009
    ..Our data suggests that MAIL is a key regulator of IL-6 production in human monocytes and plays an important role in both TLR and NOD-like receptor ligand induced inflammation...
  29. ncbi request reprint Lipopolysaccharide-induced macrophage inflammatory response is regulated by SHIP
    Huiqing Fang
    Department of Internal Medicine, Ohio State University, Columbus, OH 43210, USA
    J Immunol 173:360-6. 2004
    ..These results are the first to demonstrate a role for SHIP in TLR4 signaling, and propose that SHIP is a positive regulator of LPS-induced inflammation...
  30. pmc A synergistic role for IL-1beta and TNFalpha in monocyte-derived IFNgamma inducing activity
    RAQUEL M RAICES
    The Ohio State University, Davis Heart and Lung Research Institute, Pulmonary, Allergy, Clinical Care and Sleep Medicine Division, 473 West 12th Avenue, Room 435, Columbus, OH 43210, USA
    Cytokine 44:234-41. 2008
    ..These studies demonstrate a synergistic role between TNFalpha and IL-1 family members in the induction of IFNgamma production and give caution to interpretations of KG-1 functional assays designed to detect functional IL-18...
  31. ncbi request reprint ASC directs NF-kappaB activation by regulating receptor interacting protein-2 (RIP2) caspase-1 interactions
    Anasuya Sarkar
    Davis Heart and Lung Research Institute, The Ohio State University, Columbus 43210, USA
    J Immunol 176:4979-86. 2006
    ..These data suggest that ASC may direct caspase-1 away from RIP2-mediated NF-kappaB activation, toward caspase-1-mediated processing of proIL-1beta by interfering with the RIP2 caspase-1 interaction...
  32. pmc Microarray analysis of human monocytes infected with Francisella tularensis identifies new targets of host response subversion
    Jonathan P Butchar
    Division of Pulmonary and Critical Care, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 3:e2924. 2008
    ..This study identifies several new factors potentially important in host cell subversion by the virulent Type A F. tularensis that may serve as novel targets for drug discovery...
  33. ncbi request reprint IL-16 is constitutively present in peripheral blood monocytes and spontaneously released during apoptosis
    Andreas Elssner
    The Dorothy M Davis Heart and Lung Research Institute, Ohio State University, 473 West 12th Avenue, Columbus, OH 43210, USA
    J Immunol 172:7721-5. 2004
    ..As a specificity control, IL-1beta and IL-8 were not released during spontaneous monocyte apoptosis. In summary, our data demonstrate that monocytes contain IL-16 that is released during spontaneous apoptosis...
  34. pmc Macrophage pro-inflammatory response to Francisella novicida infection is regulated by SHIP
    Kishore V L Parsa
    The Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, USA
    PLoS Pathog 2:e71. 2006
    ..A detailed analysis of phosphoinositide signaling may provide valuable clues for better understanding the pathogenesis of tularemia...
  35. pmc Caspase-1 regulates Escherichia coli sepsis and splenic B cell apoptosis independently of interleukin-1beta and interleukin-18
    Anasuya Sarkar
    Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Am J Respir Crit Care Med 174:1003-10. 2006
    ..In this context, caspase-1 knockout mice have been shown to be protected from endotoxin-induced mortality, whereas IL-1beta knockout mice are not protected...
  36. ncbi request reprint HIV-1 infection does not impair human alveolar macrophage phagocytic function unless combined with cigarette smoking
    Andreas Elssner
    Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Chest 125:1071-6. 2004
    ..We hypothesized that HIV-1 infection may lead to a defect in the phagocytic activity of alveolar macrophages...
  37. pmc Interleukin-1beta selectively expands and sustains interleukin-22+ immature human natural killer cells in secondary lymphoid tissue
    Tiffany Hughes
    Integrated Biomedical Graduate Program, The Ohio State University, Columbus, OH 43210, USA
    Immunity 32:803-14. 2010
    ..Thus, cDC-derived IL-1beta preserves and expands IL-1R1(hi)IL-22(+)AHR(+) iNK cells, potentially influencing human mucosal innate immunity during infection...
  38. doi request reprint Failure to recover somatotropic axis function is associated with mortality from pediatric sepsis-induced multiple organ dysfunction syndrome
    David J Marquardt
    Section of Critical Care Medicine, Department of Pediatrics, Ohio State University College of Medicine, Columbus, OH, USA
    Pediatr Crit Care Med 11:18-25. 2010
    ....
  39. ncbi request reprint Respiratory symptoms among HIV-seropositive individuals
    Philip T Diaz
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, The Ohio State University, Columbus, OH, USA
    Chest 123:1977-82. 2003
    ..The current study was done to systematically identify the prevalence of respiratory symptoms among an HIV-seropositive population and to identify associated clinical features...
  40. pmc Gene expression profiling identifies MMP-12 and ADAMDEC1 as potential pathogenic mediators of pulmonary sarcoidosis
    Elliott D Crouser
    Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, The Dorothy M Davis Heart and Lung Research Institute, Columbus, Ohio 43210 1252, USA
    Am J Respir Crit Care Med 179:929-38. 2009
    ..Little is known about the genetic regulation of granulomatous inflammation in sarcoidosis...
  41. pmc Monocyte activation by necrotic cells is promoted by mitochondrial proteins and formyl peptide receptors
    Elliott D Crouser
    Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Dorothy M Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, OH, USA
    Crit Care Med 37:2000-9. 2009
    ..Thus, we hypothesized that the mitochondrial fraction of the cell, particularly N-formyl peptides, contributes significantly to the activation of monocytes by necrotic cells...

Research Grants3

  1. HIV SURVEILLANCE AFFECTS MACROPHAGE FUNCTION
    MARK WEWERS; Fiscal Year: 2002
    ..Together these experiments will improve our understanding of how the innate immune defense against bacteria is affected by attempts to suppress HIV in macrophages. ..
  2. MACROPHAGE HIV INFECTION IN THE LUNG MICROENVIRONMENT
    MARK WEWERS; Fiscal Year: 2003
    ..These studies should provide important insights into how changes in the lung microenvironment can influence disease progression. They may alter future designs for new therapeutic and antiviral treatments. ..
  3. REGULATION OF MACROPHAGE INTERLEUKIN-1 BETA PRODUCTION
    MARK WEWERS; Fiscal Year: 2006
    ..We hypothesize that MAIL, a novel protein homologue of I-kBalpha that we discovered by yeast two-hybrid screening negatively regulates prolL-1beta processing and release. ..