Robert S Munson

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. doi request reprint The enterobacterial common antigen-like gene cluster of Haemophilus ducreyi contributes to virulence in humans
    Keith E Banks
    Department of Medicine, Indiana University School of Medicine, Indianapolis, USA
    J Infect Dis 197:1531-6. 2008
  2. pmc Partial analysis of the genomes of two nontypeable Haemophilus influenzae otitis media isolates
    Robert S Munson
    Center for Microbial Pathogenesis, Columbus Children s Research Institute and The Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
    Infect Immun 72:3002-10. 2004
  3. pmc Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae
    Robert S Munson
    Division of Molecular Medicine, Children s Research Institute, and Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
    Infect Immun 72:1143-6. 2004
  4. ncbi request reprint The PilA protein of non-typeable Haemophilus influenzae plays a role in biofilm formation, adherence to epithelial cells and colonization of the mammalian upper respiratory tract
    Joseph A Jurcisek
    Columbus Children s Research Institute, The Ohio State University College of Medicine, Department of Pediatrics, Center for Microbial Pathogenesis, Columbus, OH 43205, USA
    Mol Microbiol 65:1288-99. 2007
  5. pmc Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20
    Alistair Harrison
    Center for Microbial Pathogenesis, Columbus Children s Research Institute, Ohio State University College of Medicine and Public Health, Columbus, Ohio 43205, USA
    J Bacteriol 187:4627-36. 2005
  6. pmc A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Glen McGillivary
    Columbus Children s Research Institute, Center for Microbial Pathogenesis and The Ohio State University College of Medicine and Public Health, 700 Children s Drive, Rm W591, Columbus, OH 43205, USA
    Mol Immunol 44:2446-58. 2007
  7. pmc The OxyR regulon in nontypeable Haemophilus influenzae
    Alistair Harrison
    Columbus Children s Research Institute, Center for Microbial Pathogenesis, The Ohio State University College of Medicine and Public Health, 700 Children s Drive, Columbus, OH 43205 2696, USA
    J Bacteriol 189:1004-12. 2007
  8. pmc Demonstration of Type IV pilus expression and a twitching phenotype by Haemophilus influenzae
    Lauren O Bakaletz
    Department of Pediatrics, Columbus Children s Research Institute, Center for Microbial Pathogenesis, The Ohio State University College of Medicine and Public Health, 700 Children s Dr, Columbus, OH 43205 2696, USA
    Infect Immun 73:1635-43. 2005
  9. pmc Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons
    Michael D Carruthers
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital and Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio, USA
    J Bacteriol 194:1927-33. 2012
  10. ncbi request reprint The non-typeable Haemophilus influenzae Sap transporter provides a mechanism of antimicrobial peptide resistance and SapD-dependent potassium acquisition
    Kevin M Mason
    Columbus Children s Research Institute and The Ohio State University College of Medicine and Public Health, Columbus, OH, USA
    Mol Microbiol 62:1357-72. 2006

Research Grants

  1. Genomic Sequence of an Otitis Media Isolate of NTHi
    Robert Munson; Fiscal Year: 2003
  2. Pasteurellaceae 2005
    Robert Munson; Fiscal Year: 2005
  3. Protein Glycosylatin in Haemophilus ducreyi
    Robert Munson; Fiscal Year: 2007

Collaborators

Detail Information

Publications34

  1. doi request reprint The enterobacterial common antigen-like gene cluster of Haemophilus ducreyi contributes to virulence in humans
    Keith E Banks
    Department of Medicine, Indiana University School of Medicine, Indianapolis, USA
    J Infect Dis 197:1531-6. 2008
    ..3%-70.0%) of 15 parent-strain sites and at 8.3% (95% CI, 0.01%-23.6%) of 12 mutant-strain sites (P = .013). Thus, the expression of wecA contributes to the ability of H. ducreyi to cause pustules in humans...
  2. pmc Partial analysis of the genomes of two nontypeable Haemophilus influenzae otitis media isolates
    Robert S Munson
    Center for Microbial Pathogenesis, Columbus Children s Research Institute and The Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
    Infect Immun 72:3002-10. 2004
    ..We also identified a number of putative restriction-modification systems, bacteriophage genes and transposon-related genes. These data provide new insight that complements and extends our ongoing analysis of NTHI virulence determinants...
  3. pmc Haemophilus ducreyi strain ATCC 27722 contains a genetic element with homology to the vibrio RS1 element that can replicate as a plasmid and confer NAD independence on haemophilus influenzae
    Robert S Munson
    Division of Molecular Medicine, Children s Research Institute, and Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
    Infect Immun 72:1143-6. 2004
    ..Tandem copies of the plasmid are integrated into the H. ducreyi 35000HP genome...
  4. ncbi request reprint The PilA protein of non-typeable Haemophilus influenzae plays a role in biofilm formation, adherence to epithelial cells and colonization of the mammalian upper respiratory tract
    Joseph A Jurcisek
    Columbus Children s Research Institute, The Ohio State University College of Medicine, Department of Pediatrics, Center for Microbial Pathogenesis, Columbus, OH 43205, USA
    Mol Microbiol 65:1288-99. 2007
    ..Collectively, our data demonstrate that NTHI express PilA in vivo, and that PilA plays an important role in the pathogenesis of an upper respiratory tract infection induced by NTHI...
  5. pmc Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20
    Alistair Harrison
    Center for Microbial Pathogenesis, Columbus Children s Research Institute, Ohio State University College of Medicine and Public Health, Columbus, Ohio 43205, USA
    J Bacteriol 187:4627-36. 2005
    ..These data provide new insight that complements and extends the ongoing analysis of nontypeable H. influenzae virulence determinants...
  6. pmc A member of the cathelicidin family of antimicrobial peptides is produced in the upper airway of the chinchilla and its mRNA expression is altered by common viral and bacterial co-pathogens of otitis media
    Glen McGillivary
    Columbus Children s Research Institute, Center for Microbial Pathogenesis and The Ohio State University College of Medicine and Public Health, 700 Children s Drive, Rm W591, Columbus, OH 43205, USA
    Mol Immunol 44:2446-58. 2007
    ..Collectively, these data showed that viruses and bacteria modulate AMP messages in the URT, which likely contributes to the disease course of OM...
  7. pmc The OxyR regulon in nontypeable Haemophilus influenzae
    Alistair Harrison
    Columbus Children s Research Institute, Center for Microbial Pathogenesis, The Ohio State University College of Medicine and Public Health, 700 Children s Drive, Columbus, OH 43205 2696, USA
    J Bacteriol 189:1004-12. 2007
    ....
  8. pmc Demonstration of Type IV pilus expression and a twitching phenotype by Haemophilus influenzae
    Lauren O Bakaletz
    Department of Pediatrics, Columbus Children s Research Institute, Center for Microbial Pathogenesis, The Ohio State University College of Medicine and Public Health, 700 Children s Dr, Columbus, OH 43205 2696, USA
    Infect Immun 73:1635-43. 2005
    ..The recognition that H. influenzae possesses a mechanism for twitching motility will likely profoundly influence our understanding of H. influenzae-induced diseases of the respiratory tract and their sequelae...
  9. pmc Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons
    Michael D Carruthers
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital and Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio, USA
    J Bacteriol 194:1927-33. 2012
    ....
  10. ncbi request reprint The non-typeable Haemophilus influenzae Sap transporter provides a mechanism of antimicrobial peptide resistance and SapD-dependent potassium acquisition
    Kevin M Mason
    Columbus Children s Research Institute and The Ohio State University College of Medicine and Public Health, Columbus, OH, USA
    Mol Microbiol 62:1357-72. 2006
    ..Thus, NTHI required a functional Sap system to mediate bacterial survival and pathogenesis in vivo...
  11. pmc Acinetobacter baumannii utilizes a type VI secretion system for bacterial competition
    Michael D Carruthers
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital and Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 8:e59388. 2013
    ..Collectively, our data have provided the first evidence demonstrating function in inter-bacterial competition, for a T6SS produced by A. baumannii...
  12. pmc Differential uptake and processing of a Haemophilus influenzae P5-derived immunogen by chinchilla dendritic cells
    Laura A Novotny
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, The Ohio State University College of Medicine, Columbus, OH 43205 2696, USA
    Infect Immun 76:967-77. 2008
    ..Furthermore, they suggest an important role for chinchilla DCs in the development of protective immunity against experimental NTHI-induced OM after intranasal immunization...
  13. pmc A mutation in the sap operon attenuates survival of nontypeable Haemophilus influenzae in a chinchilla model of otitis media
    Kevin M Mason
    Department of Pediatrics, Center for Microbial Pathogenesis, Columbus Children s Research Institute, The Ohio State University College of Medicine and Public Health, Columbus, OH 43205 2696, USA
    Infect Immun 73:599-608. 2005
    ..Our results indicate that the products of the sap operon are important for resisting the activity of APs and may regulate, in part, the balance between normal carriage and disease caused by NTHI...
  14. pmc Role of a GntR-family response regulator LbrA in Listeria monocytogenes biofilm formation
    Andrew Wassinger
    Department of Food Science and Technology, The Ohio State University, Columbus, Ohio, USA
    PLoS ONE 8:e70448. 2013
    ..monocytogenes. A cloned copy of Scott A lbrA was unable to confer enhanced biofilm forming potential in F2365, suggesting that additional factors contributed to weak biofilm formation by F2365. ..
  15. pmc Construction of non-polar mutants in Haemophilus influenzae using FLP recombinase technology
    Erin Tracy
    Center for Microbial Pathogenesis in The Research Institute at Nationwide Children s Hospital, Columbus, OH, USA
    BMC Mol Biol 9:101. 2008
    ..We have adapted the lambda red/FLP-recombinase-mediated strategy used in E. coli for use in NTHi...
  16. ncbi request reprint Identification and characterization of a mucosal antimicrobial peptide expressed by the chinchilla (Chinchilla lanigera) airway
    Randall H Harris
    Department of Pediatrics, College of Medicine and Public Health, The Ohio State University, Columbus Children s Research Institute, Center for Microbial Pathogenesis, 700 Children s Drive, Columbus, OH 43205 2696, USA
    J Biol Chem 279:20250-6. 2004
    ..Continued study of this and other APs will allow us to determine their role in bacterial colonization of the upper airway as well as how viruses might contribute to the pathogenesis of OM by modulating AP expression...
  17. pmc Overlapping and complementary oxidative stress defense mechanisms in nontypeable Haemophilus influenzae
    Alistair Harrison
    The Center for Microbial Pathogenesis at the Research Institute at Nationwide Children s Hospital and the Center for Microbial Interface Biology and Department of Pediatrics, The Ohio State University, Ohio, USA
    J Bacteriol 197:277-85. 2015
    ..These mechanisms will be important for successful colonization and infection by this opportunistic human pathogen. ..
  18. pmc HrrF is the Fur-regulated small RNA in nontypeable Haemophilus influenzae
    Estevan A Santana
    The Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, Columbus, Ohio, United States of America The Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America Department of Pediatrics, The Ohio State University, Columbus, Ohio, United States of America
    PLoS ONE 9:e105644. 2014
    ..The stability of HrrF is not dependent on the RNA chaperone Hfq. This study is the first step in an effort to investigate the role small RNAs play in altering gene expression in response to iron limitation in NTHi. ..
  19. pmc Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action
    Amber Nagy
    Department of Pathology, The Ohio State University, Columbus, OH, USA
    Int J Nanomedicine 6:1833-52. 2011
    ..The focus of this study is on the antibacterial properties of silver nanoparticles embedded within a zeolite membrane (AgNP-ZM)...
  20. pmc Identification and characterization of the N-acetylglucosamine glycosyltransferase gene of Haemophilus ducreyi
    Shuhua Sun
    Columbus Children s Research Institute, Department of Pediatrics, The Ohio State University, Columbus, Ohio 43205, USA
    Infect Immun 70:5887-92. 2002
    ..This strain produced the lipooligosaccharide glycoforms produced by the parental strain, confirming that the lgtA gene encodes the N-acetylglucosamine glycosyltransferase...
  21. pmc Characteristics of invasive Acinetobacter species isolates recovered in a pediatric academic center
    Avish L Jain
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, Columbus, OH, USA
    BMC Infect Dis 16:346. 2016
    ..Therefore we aimed to determine risk factors for invasive Acinetobacter infections within an academic, pediatric setting as well as defining microbiologic characteristics of predominant strains...
  22. doi request reprint Acinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O-glycosylation of multiple proteins
    Christian M Harding
    Center for Microbial Pathogenesis, The Research Institute at Nationwide Children s Hospital, Columbus, OH, USA
    Mol Microbiol 96:1023-41. 2015
    ..However, one of them is exclusively dedicated to the glycosylation of the pilin-like protein ComP. Our data reveal an intricate and remarkable evolutionary pathway for bacterial O-OTases and provide novel tools for glycoengineering...
  23. pmc Haemophilus ducreyi requires the flp gene cluster for microcolony formation in vitro
    Joseph R Nika
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Infect Immun 70:2965-75. 2002
    ..These results indicate that the flp gene cluster is essential for microcolony formation by H. ducreyi, whereas this phenotypic trait is not linked to the virulence potential of the pathogen, at least in this animal model of infection...
  24. ncbi request reprint H. influenzae Consortium: integrative study of H. influenzae-human interactions
    Eugene Kolker
    BIATECH, 19310 North Creek Parkway, Suite 115, Bothell, WA 98011, USA
    OMICS 6:341-8. 2002
    ..Data from the HIC will allow greater understanding of cellular behavior, pathogen-host interactions, bacterial infection, and provide future scientific endeavors with a template for studies of other pathogens...
  25. ncbi request reprint Proteome of Haemophilus ducreyi by 2-D SDS-PAGE and mass spectrometry: strain variation, virulence, and carbohydrate expression
    N Karoline Scheffler
    Buck Institute for Age Research, Novato, California 94945, USA
    J Proteome Res 2:523-33. 2003
    ..This African strain may represent a prototype of a second clonal group of H. ducreyi...
  26. pmc Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae
    Eugene Kolker
    BIATECH, 19310 North Creek Parkway, Suite 115, Bothell, WA 98011, USA
    Nucleic Acids Res 32:2353-61. 2004
    ....
  27. pmc Identification of genes involved in the expression of atypical lipooligosaccharide structures from a second class of Haemophilus ducreyi
    Deborah M B Post
    Buck Institute for Age Research, 8001 Redwood Blvd, Novato, CA 94945, USA
    Infect Immun 75:113-21. 2007
    ..These data indicate that the lbgAB genes can serve as one indicator of the classification of H. ducreyi strains...
  28. pmc Identification of a novel sialic acid transporter in Haemophilus ducreyi
    Deborah M B Post
    Buck Institute for Age Research, Novato, CA 94945, USA
    Infect Immun 73:6727-35. 2005
    ..Uptake assays using radiolabeled sialic acid confirmed that the mutants were unable to transport sialic acid. This study is the first report of bacteria using an ABC transporter for sialic acid uptake...
  29. pmc Identification of Haemophilus ducreyi genes expressed during human infection
    Margaret E Bauer
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Microbiology 154:1152-60. 2008
    ..We identified three genes, lspA1, lspA2 and tadA, known to be required for virulence in humans. This is the first study to broadly define transcripts expressed by H. ducreyi in humans...
  30. pmc Nontypeable Haemophilus influenzae gene expression induced in vivo in a chinchilla model of otitis media
    Kevin M Mason
    Columbus Children s Research Institute, Department of Pediatrics, The Ohio State University College of Medicine and Public Health, Columbus 43205, USA
    Infect Immun 71:3454-62. 2003
    ..These data provide insight into the response of NTHI bacteria as they sense and respond to the middle ear microenvironment during early events of otitis media...
  31. pmc Sequence and functional analyses of Haemophilus spp. genomic islands
    Mario Juhas
    Clinical Microbiology and Infectious Diseases, NDCLS, University of Oxford, Headley Way, Oxford OX3 9DU, UK
    Genome Biol 8:R237. 2007
    ..More clarity comes from this comparative analysis of seven complete sequences of the ICEHin1056 genomic island subfamily...
  32. pmc Immunopathogenesis of Haemophilus ducreyi infection (chancroid)
    Stanley M Spinola
    Department of Medicine, School of Medicine, Indiana University, Indianapolis, Indiana 46202, USA
    Infect Immun 70:1667-76. 2002
  33. ncbi request reprint Regulation of sialic acid transport and catabolism in Haemophilus influenzae
    Jason W Johnston
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Mol Microbiol 66:26-39. 2007
    ..Our results demonstrate the importance of SiaR-mediated regulation to balance the requirement of surface sialylation and the toxic accumulation of intracellular sialic acid...
  34. ncbi request reprint Haemophilus influenzae type b strain A2 has multiple sialyltransferases involved in lipooligosaccharide sialylation
    Paul A Jones
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 277:14598-611. 2002
    ..A triple mutant of siaA, lic3A, and lsgB contained no sialylated glycoforms. These results demonstrate that the sialylation of the LOS of H. influenzae is a complex process involving multiple sialyltransferases...

Research Grants3

  1. Genomic Sequence of an Otitis Media Isolate of NTHi
    Robert Munson; Fiscal Year: 2003
    ..We propose to close and annotate the genome of nontypeable H. influenzae strain 86-028. ..
  2. Pasteurellaceae 2005
    Robert Munson; Fiscal Year: 2005
    ..These 2 activities will assure that all of our conference goals are met. ..
  3. Protein Glycosylatin in Haemophilus ducreyi
    Robert Munson; Fiscal Year: 2007
    ..ducreyi infection. [unreadable] [unreadable] [unreadable]..