- In vitro reconstitution of the human RISC-loading complexIan J Macrae
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 105:512-7. 2008..Furthermore, once Ago2 is loaded with an miRNA, it tends to dissociate from the rest of the complex. These results lay the groundwork for future structural and functional dissection of RISC loading in humans...
- Single-pot enzymatic synthesis of Dicer-substrate siRNAsKeelan Z Guiley
Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA
Nucleic Acids Res 40:e40. 2012..The overall reaction is simple, can be carried out in any laboratory with access to a PCR machine, and is amenable to high-throughput processes...
- Preparation and characterization of the extracellular domain of human Sid-1Ashley J Pratt
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America
PLoS ONE 7:e33607. 2012..These characterizations provide inroads towards understanding the mechanism of this unique RNA transport system from structural prospective...