Daniel L Kaplan
Affiliation: The Rockefeller University
- Mcm4,6,7 uses a "pump in ring" mechanism to unwind DNA by steric exclusion and actively translocate along a duplexDaniel L Kaplan
Rockefeller University and Howard Hughes Medical Institute, Laboratory of DNA Replication, New York, New York 10021, USA
J Biol Chem 278:49171-82. 2003....
- DnaB drives DNA branch migration and dislodges proteins while encircling two DNA strandsDaniel L Kaplan
Laboratory of DNA Replication, Rockefeller University, New York, NY 10021, USA
Mol Cell 10:647-57. 2002..DnaB binds to just one DNA strand during branch migration. T7 phage gp4 protein also drives DNA branch migration, suggesting this activity generalizes to other ring-shaped helicases...
- Twin DNA pumps of a hexameric helicase provide power to simultaneously melt two duplexesDaniel L Kaplan
Rockefeller University, New York, NY 10021, USA
Mol Cell 15:453-65. 2004..Finally, we present evidence that the DNA binding site of DnaB is buried inside its central channel...
- RuvA is a sliding collar that protects Holliday junctions from unwinding while promoting branch migrationDaniel L Kaplan
Rockefeller University, Laboratory of DNA Replication, New York, NY 10021, USA
J Mol Biol 355:473-90. 2006..Finally, we show that RuvA is less mobile at a heterologous junction compared to a homologous junction, as two opposing DnaB pumps are required to mobilize RuvA over heterologous DNA...
- Rho factor: transcription termination in four stepsDaniel L Kaplan
Rockefeller University, Laboratory of DNA Replication, Box 228, 1230 York Avenue, New York, NY 10021, USA
Curr Biol 13:R714-6. 2003..Rho factor is a hexameric ring-shaped helicase which terminates transcription in Escherichia coli. Two recent crystal structures of Rho in complex with nucleic acid reveal how this helicase ring loads onto mRNA and encircles it...
- Replication termination: mechanism of polar arrest revealedDaniel L Kaplan
Vanderbilt University, Department of Biological Sciences, VU Station B, Box 35 1634, Nashville, Tennessee 37235, USA
Curr Biol 16:R684-6. 2006..As the replication fork helicase unwinds one side of Ter, a conserved cytosine flips out of the duplex and binds to Tus, thereby creating a locked complex that blocks the advancing helicase...