Nathaniel Heintz

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. ncbi request reprint The folate metabolic enzyme ALDH1L1 is restricted to the midline of the early CNS, suggesting a role in human neural tube defects
    Todd E Anthony
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York, USA
    J Comp Neurol 500:368-83. 2007
  2. ncbi request reprint Biomedicine. Ataxin-1 regulators in the spotlight
    Nathaniel Heintz
    Department of Molecular Biology, Rockefeller University, New York, NY 10021, USA
    Science 301:59-60. 2003
  3. ncbi request reprint Modified bacterial artificial chromosomes for zebrafish transgenesis
    Zhongan Yang
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095 1606, USA
    Methods 39:183-8. 2006
  4. pmc Application of a translational profiling approach for the comparative analysis of CNS cell types
    Joseph P Doyle
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Cell 135:749-62. 2008
  5. pmc Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis
    Shiaoching Gong
    The Rockefeller University, New York, New York, USA
    Nat Protoc 5:1678-96. 2010
  6. pmc Genetic lineage tracing defines distinct neurogenic and gliogenic stages of ventral telencephalic radial glial development
    Todd E Anthony
    Laboratory of Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Neural Dev 3:30. 2008
  7. pmc Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells
    Todd E Anthony
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Genes Dev 19:1028-33. 2005
  8. pmc A role for LYNX2 in anxiety-related behavior
    Ayse B Tekinay
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:4477-82. 2009
  9. pmc Cholinergic interneurons in the nucleus accumbens regulate depression-like behavior
    Jennifer L Warner-Schmidt
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:11360-5. 2012
  10. pmc The biochemical anatomy of cortical inhibitory synapses
    Elizabeth A Heller
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 7:e39572. 2012

Research Grants

Collaborators

Detail Information

Publications46

  1. ncbi request reprint The folate metabolic enzyme ALDH1L1 is restricted to the midline of the early CNS, suggesting a role in human neural tube defects
    Todd E Anthony
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York, USA
    J Comp Neurol 500:368-83. 2007
    ..Moreover, a role for loss-of-function mutations in human NTDs remains possible, as Mthfr null mice do not develop NTDs even though MTHFR mutations increase human NTD risk...
  2. ncbi request reprint Biomedicine. Ataxin-1 regulators in the spotlight
    Nathaniel Heintz
    Department of Molecular Biology, Rockefeller University, New York, NY 10021, USA
    Science 301:59-60. 2003
  3. ncbi request reprint Modified bacterial artificial chromosomes for zebrafish transgenesis
    Zhongan Yang
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095 1606, USA
    Methods 39:183-8. 2006
    ..Experimental procedures for BAC DNA preparation, microinjection of zebrafish embryos and screening of transgenic zebrafish carrying GFP/RFP modified BAC clones are detailed...
  4. pmc Application of a translational profiling approach for the comparative analysis of CNS cell types
    Joseph P Doyle
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Cell 135:749-62. 2008
    ..This resource will enable a wide spectrum of molecular and mechanistic studies of both well-known and previously uncharacterized neural cell populations...
  5. pmc Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis
    Shiaoching Gong
    The Rockefeller University, New York, New York, USA
    Nat Protoc 5:1678-96. 2010
    ..CNS anatomical expression maps of the mice are available to the public at http://www.gensat.org/...
  6. pmc Genetic lineage tracing defines distinct neurogenic and gliogenic stages of ventral telencephalic radial glial development
    Todd E Anthony
    Laboratory of Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Neural Dev 3:30. 2008
    ....
  7. pmc Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells
    Todd E Anthony
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Genes Dev 19:1028-33. 2005
    ..These results identify Blbp as the first predominantly CNS-specific Notch target gene and suggest that it mediates some aspects of Notch signaling in radial glia...
  8. pmc A role for LYNX2 in anxiety-related behavior
    Ayse B Tekinay
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:4477-82. 2009
    ....
  9. pmc Cholinergic interneurons in the nucleus accumbens regulate depression-like behavior
    Jennifer L Warner-Schmidt
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:11360-5. 2012
    ....
  10. pmc The biochemical anatomy of cortical inhibitory synapses
    Elizabeth A Heller
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 7:e39572. 2012
    ....
  11. pmc Proteomic studies of a single CNS synapse type: the parallel fiber/purkinje cell synapse
    Fekrije Selimi
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, USA
    PLoS Biol 7:e83. 2009
    ..Our data identify a novel synapse-specific signaling pathway, and provide an approach for detailed investigations of the biochemical complexity of central nervous system synapse types...
  12. pmc Mouse transgenesis in a single locus with independent regulation for multiple fluorophores
    Joseph D Dougherty
    Laboratory of Molecular Biology, The Rockefeller University, New York, New York, United States of America
    PLoS ONE 7:e40511. 2012
    ..This generic solution should permit more sophisticated experimental manipulations to assess functional interactions amongst populations of cell types in vivo in a more rapid and efficient manner...
  13. pmc Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex
    Nicole C McKnight
    Department of Neurology and Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America
    PLoS Genet 10:e1004626. 2014
    ....
  14. ncbi request reprint A novel family of transmembrane proteins interacting with beta subunits of the Na,K-ATPase
    Svetlana Gorokhova
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Hum Mol Genet 16:2394-410. 2007
    ..The neuronal expression of NKAIN proteins, their membrane localization and the temperature-sensitive paralysis of NKAIN Drosophila mutants strongly suggest that this novel protein family may be critical for neuronal function...
  15. pmc MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system
    Marian Mellén
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 151:1417-30. 2012
    ..These findings support a model in which 5hmC and MeCP2 constitute a cell-specific epigenetic mechanism for regulation of chromatin structure and gene expression...
  16. ncbi request reprint The prototoxin lynx1 acts on nicotinic acetylcholine receptors to balance neuronal activity and survival in vivo
    Julie M Miwa
    The Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Neuron 51:587-600. 2006
    ..We conclude that lynx1 functions as an allosteric modulator of nAChR function in vivo, balancing neuronal activity and survival in the CNS...
  17. ncbi request reprint Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs
    Shiaoching Gong
    Gene Expression Nervous System Atlas Project, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Neurosci 27:9817-23. 2007
  18. ncbi request reprint A gene expression atlas of the central nervous system based on bacterial artificial chromosomes
    Shiaoching Gong
    GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA
    Nature 425:917-25. 2003
    ..The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community...
  19. ncbi request reprint Dysfunction of axonemal dynein heavy chain Mdnah5 inhibits ependymal flow and reveals a novel mechanism for hydrocephalus formation
    Ines Ibanez-Tallon
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA
    Hum Mol Genet 13:2133-41. 2004
    ..The higher incidence of aqueduct stenosis and hydrocephalus formation in patients with ciliary defects proves the relevance of this novel mechanism in humans...
  20. pmc Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor
    Zhenyu Yue
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:15077-82. 2003
    ..They both provide a biological explanation for recent evidence implicating beclin 1 in human cancer and suggest that mutations in other genes operating in this pathway may contribute to tumor formation through deregulation of autophagy...
  21. pmc A translational profiling approach for the molecular characterization of CNS cell types
    Myriam Heiman
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, NY 10065, USA
    Cell 135:738-48. 2008
    ....
  22. pmc Analytical approaches to RNA profiling data for the identification of genes enriched in specific cells
    Joseph D Dougherty
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Nucleic Acids Res 38:4218-30. 2010
    ..We apply this measure to a large survey of CNS cell-specific microarray data to identify those genes that are significantly enriched in each population Data and algorithms are available online (www.bactrap.org)...
  23. pmc Fate mapping for activation-induced cytidine deaminase (AID) marks non-lymphoid cells during mouse development
    Philipp C Rommel
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York, USA
    PLoS ONE 8:e69208. 2013
    ..Finally, in the adult mouse brain, AID(cre) marks a small fraction of diverse neurons and distinct neuronal populations, including pyramidal cells in cortical layer IV. ..
  24. pmc Cell type-specific regulation of DARPP-32 phosphorylation by psychostimulant and antipsychotic drugs
    Helen S Bateup
    Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, 1230 York Ave, New York, New York 10065, USA
    Nat Neurosci 11:932-9. 2008
    ..Furthermore, we found that a variety of drugs that target the striatum have cell type-specific effects that previous methods were not able to discern...
  25. pmc Distinct regulation of autophagic activity by Atg14L and Rubicon associated with Beclin 1-phosphatidylinositol-3-kinase complex
    Yun Zhong
    1 Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA 2 These authors contributed equally to the work
    Nat Cell Biol 11:468-76. 2009
    ..We hypothesize that by forming distinct protein complexes, Beclin 1 and its binding proteins orchestrate the precise function of the class III PI(3)K in regulating autophagy at multiple steps...
  26. ncbi request reprint Large-scale genomic approaches to brain development and circuitry
    Mary E Hatten
    Laboratory of Developmental Neurobiology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Annu Rev Neurosci 28:89-108. 2005
    ..In this review, we summarize current phenotype- and genotype-driven approaches to discover novel CNS-expressed genes, as well as current approaches to carry out large-scale, gene-expression screens in the CNS...
  27. ncbi request reprint Distribution of Kv3.3 potassium channel subunits in distinct neuronal populations of mouse brain
    Su Ying Chang
    Department of Physiology and Neuroscience, New York University School of Medicine, New York, New York 10016, USA
    J Comp Neurol 502:953-72. 2007
    ..In this study, we identified additional populations and subcellular compartments that are likely to sustain high-frequency firing because of the expression of Kv3.3 and other Kv3 subunits...
  28. pmc Identification of the cortical neurons that mediate antidepressant responses
    Eric F Schmidt
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    Cell 149:1152-63. 2012
    ..Our data identify corticostriatal projection neurons as critical for the response to antidepressants, and suggest that the regulation of serotonergic tone in this single cell type plays a pivotal role in antidepressant therapy...
  29. pmc A Cortical Circuit for Sexually Dimorphic Oxytocin-Dependent Anxiety Behaviors
    Kun Li
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 167:60-72.e11. 2016
    ..They suggest that additional studies of the impact of the OXT/OXTR and CRHBP/CRH pathways in males and females will be important in development of gender-specific therapies...
  30. ncbi request reprint Induction of autophagy in axonal dystrophy and degeneration
    Qing Jun Wang
    Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, New York 10021, USA
    J Neurosci 26:8057-68. 2006
    ..We propose that induction of autophagy serves as an early stress response in axonal dystrophy and may participate in the remodeling of axon structures...
  31. pmc Oxytocin modulates female sociosexual behavior through a specific class of prefrontal cortical interneurons
    Miho Nakajima
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 159:295-305. 2014
    ..Our data demonstrate a gender-, cell type-, and state-specific role for OT/Oxtr signaling in the mPFC and identify a latent cortical circuit element that may modulate other complex social behaviors in response to OT...
  32. pmc Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP)
    Myriam Heiman
    1 Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, USA 2
    Nat Protoc 9:1282-91. 2014
    ..This protocol provides a step-by-step guide to implement the TRAP methodology, which takes 2 d to complete once all materials are in hand. ..
  33. ncbi request reprint Tethering naturally occurring peptide toxins for cell-autonomous modulation of ion channels and receptors in vivo
    Ines Ibanez-Tallon
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Neuron 43:305-11. 2004
    ..When combined with specific methods of cell and temporal expression, the extension of this approach to hundreds of naturally occurring peptide toxins opens a new landscape for cell-autonomous regulation of cellular physiology in vivo...
  34. ncbi request reprint Radial glia serve as neuronal progenitors in all regions of the central nervous system
    Todd E Anthony
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Neuron 41:881-90. 2004
    ..Thus, radial glial populations within different CNS regions are not heterogeneous with regard to their potential to generate neurons versus glia...
  35. ncbi request reprint Gene expression nervous system atlas (GENSAT)
    Nathaniel Heintz
    The Rockefeller University and Howard Hughes Medical Institute, 1230 York Avenue, New York, New York 10021, USA
    Nat Neurosci 7:483. 2004
  36. pmc Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replication
    Shiaoching Gong
    Laboratory of Molecular Biology, The Rockefeller University, New York, New York 10021, USA
    Genome Res 12:1992-8. 2002
    ..This new system has been used to modify a dozen BACs. It is well suited for efficient production of modified BACs for use in a variety of in vivo studies...
  37. ncbi request reprint A novel protein complex linking the delta 2 glutamate receptor and autophagy: implications for neurodegeneration in lurcher mice
    Zhenyu Yue
    Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Neuron 35:921-33. 2002
    ..These results provide strong evidence that a direct link exists between GluRdelta2(Lc) receptor and stimulation of the autophagic pathway in dying lurcher Purkinje cells...
  38. ncbi request reprint To beat or not to beat: roles of cilia in development and disease
    Ines Ibanez-Tallon
    Howard Hughes Medical Institute, The Rockefeller University, Laboratory of Molecular Biology, New York, NY 10021, USA
    Hum Mol Genet 12:R27-35. 2003
    ..We also consider evolutionary relationships between cilia from lower and higher eukaryotes, outline the ciliary components required for assembly and motility, and review the terminology of axonemal heavy chain dynein genes...
  39. pmc The nuclear DNA base 5-hydroxymethylcytosine is present in Purkinje neurons and the brain
    Skirmantas Kriaucionis
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    Science 324:929-30. 2009
    ..2% in granule cells, and is not present in cancer cell lines. hmdC is a constituent of nuclear DNA that is highly abundant in the brain, suggesting a role in epigenetic control of neuronal function...
  40. ncbi request reprint Loss of function of axonemal dynein Mdnah5 causes primary ciliary dyskinesia and hydrocephalus
    Ines Ibanez-Tallon
    Howard Hughes Medical Institute, Laboratory of Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Hum Mol Genet 11:715-21. 2002
    ..Comparison of the mouse model and the human data suggests that the degree of ciliary dysfunction is causally related to the severity of human PCD, particularly the presence of hydrocephalus...
  41. ncbi request reprint Pathological cell-cell interactions elicited by a neuropathogenic form of mutant Huntingtin contribute to cortical pathogenesis in HD mice
    Xiaofeng Gu
    Center for Neurobehavioral Genetics, Neuropsychiatric Institute, Department of Psychiatry, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Neuron 46:433-44. 2005
    ..Our study provides genetic evidence that pathological cell-cell interactions elicited by neuropathogenic forms of mhtt can critically contribute to cortical pathogenesis in a HD mouse model...
  42. ncbi request reprint Cre-ating somatic cell genetic mosaics in the mouse
    Todd E Anthony
    Cell 121:322-3. 2005
    ..In this issue of Cell, describe a novel method called MADM that allows the generation of such mosaics in mice...
  43. ncbi request reprint How neurons keep in touch
    Fekrije Selimi
    Nat Neurosci 8:1417-8. 2005
  44. ncbi request reprint Autophagy and its possible roles in nervous system diseases, damage and repair
    David C Rubinsztein
    Departments of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, UK
    Autophagy 1:11-22. 2005
    ..While many issues remain unresolved, these conditions raise the possibility that autophagy can have either deleterious or protective effects depending on the specific situation and stage in the pathological process...
  45. pmc In vivo activation of midbrain dopamine neurons via sensitized, high-affinity alpha 6 nicotinic acetylcholine receptors
    Ryan M Drenan
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Neuron 60:123-36. 2008
    ..alpha6*-specific agonists or antagonists may, by targeting endogenous cholinergic mechanisms in midbrain or striatum, provide a method for manipulating DA transmission in neural disorders...
  46. ncbi request reprint Defining the expression pattern of the LGI1 gene in BAC transgenic mice
    Karen Head
    Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York 14263, USA
    Mamm Genome 18:328-37. 2007
    ..All other major organs analyzed were negative. The pattern of reporter gene expression was identical in three individual founder mice, arguing against a position effect altering expression profile due to the integration site of the BAC...

Research Grants2

  1. THE USE OF BACS FOR ANALYSIS OF CNS SPECIFIC GENES
    Nathaniel Heintz; Fiscal Year: 2001
    ..abstract_text> ..
  2. Development of Tethered Toxins for Neuroscience Research
    Nathaniel Heintz; Fiscal Year: 2006
    ..These studies will allow unprecedented precision in the genetic dissection of functions required for CNS development, function and dysfunction in vivo. ..