Klaus M Hahn

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI
    Miguel Angel del Pozo
    Division of Vascular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 4:232-9. 2002
  2. ncbi request reprint Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
  3. ncbi request reprint Live-cell fluorescent biosensors for activated signaling proteins
    Klaus Hahn
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC162, La Jolla, CA 92037, USA
    Curr Opin Cell Biol 14:167-72. 2002
  4. ncbi request reprint Monitoring signaling processes in living cells using biosensors
    Klaus Hahn
    Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Sci STKE 2003:tr5. 2003
  5. pmc GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
  6. pmc Coordination of Rho GTPase activities during cell protrusion
    Matthias Machacek
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:99-103. 2009
  7. ncbi request reprint Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein beta-catenin with the scaffolding protein MAGI-2
    M Cecilia Subauste
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    J Biol Chem 280:5676-81. 2005
  8. ncbi request reprint Designing biosensors for Rho family proteins--deciphering the dynamics of Rho family GTPase activation in living cells
    Olivier Pertz
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Cell Sci 117:1313-8. 2004
  9. pmc RhoA GTPase activation by TLR2 and TLR3 ligands: connecting via Src to NF-kappa B
    Maria Manukyan
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 182:3522-9. 2009
  10. pmc Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility
    M Cecilia Subauste
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 165:371-81. 2004

Collaborators

Detail Information

Publications29

  1. ncbi request reprint Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI
    Miguel Angel del Pozo
    Division of Vascular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 4:232-9. 2002
    ..Release of Rho-GDI after membrane translocation allows Rac to bind to effectors. Thus, Rho-GDI confers spatially restricted regulation of Rac-effector interactions...
  2. ncbi request reprint Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
    ..Our data therefore show that different extracellular cues induce distinct patterns of RhoA signalling during membrane protrusion...
  3. ncbi request reprint Live-cell fluorescent biosensors for activated signaling proteins
    Klaus Hahn
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC162, La Jolla, CA 92037, USA
    Curr Opin Cell Biol 14:167-72. 2002
    ..Alternate approaches can report post-translational modifications, ligand interactions and conformational changes, revealing how the location and subtle timing of protein activity controls cell behavior...
  4. ncbi request reprint Monitoring signaling processes in living cells using biosensors
    Klaus Hahn
    Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Sci STKE 2003:tr5. 2003
    ..Several of the animations describe the production of a fluorescent resonance energy transfer (FRET) signal...
  5. pmc GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
    ..Our results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases...
  6. pmc Coordination of Rho GTPase activities during cell protrusion
    Matthias Machacek
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Nature 461:99-103. 2009
    ....
  7. ncbi request reprint Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein beta-catenin with the scaffolding protein MAGI-2
    M Cecilia Subauste
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    J Biol Chem 280:5676-81. 2005
    ..These studies indicate that PTEN protein levels are dependent on the maintenance of beta-catenin-MAGI-2 interaction, in which vinculin plays a critical role...
  8. ncbi request reprint Designing biosensors for Rho family proteins--deciphering the dynamics of Rho family GTPase activation in living cells
    Olivier Pertz
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Cell Sci 117:1313-8. 2004
    ..Several strategies can be used to construct such probes, and there are advantages and disadvantages associated with the diverse probe designs...
  9. pmc RhoA GTPase activation by TLR2 and TLR3 ligands: connecting via Src to NF-kappa B
    Maria Manukyan
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 182:3522-9. 2009
    ..These results suggest that RhoA plays a role downstream of MyD88-dependent and -independent TLR signaling and acts as a molecular switch downstream of TLR-Src-initiated pathways...
  10. pmc Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility
    M Cecilia Subauste
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 165:371-81. 2004
    ..The enhanced motility of vin-/- and vinY822F cells is also shown to be due to a similar mechanism. Thus, vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction...
  11. ncbi request reprint A theoretical study of the UV/visible absorption and emission solvatochromic properties of solvent-sensitive dyes
    Wen Ge Han
    Department of Molecular Biology, TPC 15, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Chemphyschem 4:1084-94. 2003
    ..The geometries, electronic structures, dipole moments, and intra- and intermolecular charge transfers of the dyes in different solvents are also discussed...
  12. ncbi request reprint Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility
    Alexei Toutchkine
    Department of Cell Biology, Scripps Research Institute, 10550, N Torrey Pines Road, BCC162, La Jolla, California 92037, USA
    Bioconjug Chem 13:387-91. 2002
    ..These novel derivatives have good water solubility (>2.5 mM) and bear only one reactive side chain, reducing possible protein cross-linking encountered with previous derivatives...
  13. pmc Effects of cell tension on the small GTPase Rac
    Akira Katsumi
    Division of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 158:153-64. 2002
    ..We hypothesize that regulation of Rac activity by tension may be important for motility, polarization, and directionality of cell movement...
  14. ncbi request reprint Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis
    Elisabeth M Gardiner
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Biol 12:2029-34. 2002
    ..These data establish that Rac GTPase is spatially and temporally regulated to coordinate leading-edge extension and tail retraction during a complex motile response, the chemotaxis of human neutrophils...
  15. pmc Experimental and DFT studies: novel structural modifications greatly enhance the solvent sensitivity of live cell imaging dyes
    Alexei Toutchkine
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Phys Chem A 111:10849-60. 2007
    ..Time-dependent DFT calculations were also performed on AI-BA(4); these were less accurate than the VSCRF method in predicting the absorption energy shift from benzene to methanol...
  16. pmc A biosensor of S100A4 metastasis factor activation: inhibitor screening and cellular activation dynamics
    Sarah C Garrett
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:986-96. 2008
    ..These data demonstrate the utility of the new biosensor both for drug discovery and for probing the cellular dynamics controlled by the S100A4 metastasis factor...
  17. ncbi request reprint Design and optimization of genetically encoded fluorescent biosensors: GTPase biosensors
    Louis Hodgson
    Department of Pharmacology, Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Methods Cell Biol 85:63-81. 2008
    ....
  18. pmc RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells
    Mirvat El-Sibai
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Exp Cell Res 314:1540-52. 2008
    ..These data describe a novel role for Rho in coordinating signaling by Rac and Cdc42...
  19. pmc Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells
    Mirvat El-Sibai
    Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 120:3465-74. 2007
    ..Our data suggest that Cdc42 activation is crucial for the regulation of actin polymerization in carcinoma cells, and required for both EGF-stimulated protrusion and cell motility independently of effects on Rac...
  20. pmc Simple one-pot preparation of water-soluble, cysteine-reactive cyanine and merocyanine dyes for biological imaging
    Alexei Toutchkine
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Bioconjug Chem 18:1344-8. 2007
    ..Both iodo- and chloroacetamido derivatives were shown to be useful in protein labeling. Less reactive chloroacetamides will be preferential for selective labeling of the most reactive cysteines...
  21. ncbi request reprint Activation of endogenous Cdc42 visualized in living cells
    Perihan Nalbant
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7365, USA
    Science 305:1615-9. 2004
    ....
  22. pmc PB1 domain-dependent signaling complex is required for extracellular signal-regulated kinase 5 activation
    Kazuhiro Nakamura
    Department of Pharmacology, CB 7365, 1108 Mary Ellen Jones Building, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Mol Cell Biol 26:2065-79. 2006
    ..The MEK5 PB1 domain confers stringent MAP3K regulation of ERK5 relative to more promiscuous MAP3K control of ERK1/2, JNK, and p38...
  23. ncbi request reprint Combining surface chemistry with a FRET-based biosensor to study the dynamics of RhoA GTPase activation in cells on patterned substrates
    Louis Hodgson
    Department of Chemistry, Carolina Center for Genome Science, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    J Am Chem Soc 129:9264-5. 2007
  24. doi request reprint Digital differential interference contrast autofocus for high-resolution oil-immersion microscopy
    Feimo Shen
    Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Cytometry A 73:658-66. 2008
    ....
  25. pmc To stabilize neutrophil polarity, PIP3 and Cdc42 augment RhoA activity at the back as well as signals at the front
    Alexandra Van Keymeulen
    Department of Cellular and Molecular Pharmacology, and Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 174:437-45. 2006
    ..Thus, PIP3 and Cdc42 maintain stable polarity with a single front and a single back not only by strengthening pseudopods but also, at longer range, by promoting RhoA-dependent actomyosin contraction at the trailing edge...
  26. pmc Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells
    Christian Frantz
    Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 179:403-10. 2007
    ....
  27. pmc Spatial and temporal regulation of focal adhesion kinase activity in living cells
    Xinming Cai
    Department of Cell and Developmental Biology, 534 Taylor Hall, CB 7090, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell Biol 28:201-14. 2008
    ..These findings provide direct evidence of conformational regulation of FAK in living cells and novel insight into the mechanism regulating FAK conformation...
  28. pmc DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms
    Kevin D Healy
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC 27599 7295, USA
    Mol Carcinog 47:326-37. 2008
    ..Combined, these studies provide information on the mechanism of DLC-1 function and regulation, and further support the role of DLC-1 tumor suppression in NSCLC...
  29. pmc From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006
    Yu li Wang
    Department of Physiology, University of Massachusetts Medical School, Worcester, 01655, USA
    J Cell Biol 174:481-4. 2006
    ..The goal was to turn live cell imaging from a "technique" used in cell biology into a new exploratory science that combines a number of research fields...