James Cowan

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. ncbi request reprint Iron sulfur cluster biosynthesis. Human NFU mediates sulfide delivery to ISU in the final step of [2Fe-2S] cluster assembly
    Yushi Liu
    Evans Laboratory of Chemistry, Ohio State University, 100 West, 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) . 2007
  2. pmc Glutathione-coordinated [2Fe-2S] cluster: a viable physiological substrate for mitochondrial ABCB7 transport
    Jingwei Li
    Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 51:2253-5. 2015
  3. pmc Analysis of RNA cleavage by MALDI-TOF mass spectrometry
    Jeff C Joyner
    Department of Chemistry and Biochemistry, Evans Laboratory of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Nucleic Acids Res 41:e2. 2013
  4. pmc A structural model for glutathione-complexed iron-sulfur cluster as a substrate for ABCB7-type transporters
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, USA
    Chem Commun (Camb) 50:3795-8. 2014
  5. pmc Target-directed catalytic metallodrugs
    J C Joyner
    Evans Laboratory of Chemistry, Ohio State University, Columbus, OH 43210, USA
    Braz J Med Biol Res 46:465-85. 2013
  6. pmc Glutathione-complexed iron-sulfur clusters. Reaction intermediates and evidence for a template effect promoting assembly and stability
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 49:6313-5. 2013
  7. pmc Human ferredoxin-2 displays a unique conformational change
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA
    Dalton Trans 42:3088-91. 2013
  8. pmc Antimicrobial metallopeptides with broad nuclease and ribonuclease activity
    Jeff C Joyner
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Chem Commun (Camb) 49:2118-20. 2013
  9. doi request reprint DNA nuclease activity of Rev-coupled transition metal chelates
    Jeff C Joyner
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Dalton Trans 41:6567-78. 2012
  10. doi request reprint Catalytic metallodrugs targeting HCV IRES RNA
    Seth Bradford
    Department of Chemistry, The Ohio State University, 100 W, 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 48:3118-20. 2012

Research Grants

  1. Novel Inorganic Nucleases
    James Cowan; Fiscal Year: 2006
  2. Novel Engineered Synthetic Metallodrugs Directed Against HCV
    San Ching; Fiscal Year: 2008

Collaborators

  • T Ohyama
  • A Agarwal
  • Russ Hille
  • Yong Xiong
  • Michael E Maguire
  • Wenbin Qi
  • Jeff C Joyner
  • Sheref S Mansy
  • Yan Jin
  • Jingwei Li
  • Nikhil H Gokhale
  • Seth Bradford
  • Yushi Liu
  • San Ching
  • Taejin Yoon
  • Shu Pao Wu
  • Manunya Nuth
  • Gong Wu
  • J C Joyner
  • Kevin D Keuper
  • A Sreedhara
  • Anjali Patwardhan
  • Jia Huang
  • Kristene K Surerus
  • Eric Dizin
  • Chun An Chen
  • Alavattam Sreedhara
  • Rachel Boyd
  • C Y Chain
  • Deepika Tamuly
  • W F Hodnick
  • A F Pasquevich
  • ADA S COWAN
  • G A Pasquevich
  • U Puapaiboon
  • Yuta Kawarasaki
  • Eric C Long
  • Mark A Lewis
  • D Li
  • Shu pao Wu Sp
  • Craig Hemann
  • Matthew W Foster
  • M Sundaralingam
  • A Soriano
  • J Jai-Nhuknan
  • S Bian
  • C E Cottrell
  • J Tan
  • L R Helms
  • R P Swenson

Detail Information

Publications52

  1. ncbi request reprint Iron sulfur cluster biosynthesis. Human NFU mediates sulfide delivery to ISU in the final step of [2Fe-2S] cluster assembly
    Yushi Liu
    Evans Laboratory of Chemistry, Ohio State University, 100 West, 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) . 2007
    ..Human NFU forms a complex with NifS-like proteins and is a functionally competent reducing agent for cysteinyl persulfide bond cleavage, releasing inorganic sulfide for incorporation into the ISU-bound [2Fe-2S] cluster...
  2. pmc Glutathione-coordinated [2Fe-2S] cluster: a viable physiological substrate for mitochondrial ABCB7 transport
    Jingwei Li
    Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 51:2253-5. 2015
    ..Flow cytometry and colorimetric assays demonstrate a quantitative methodology for study of metal translocation proteins and their proteoliposome products. ..
  3. pmc Analysis of RNA cleavage by MALDI-TOF mass spectrometry
    Jeff C Joyner
    Department of Chemistry and Biochemistry, Evans Laboratory of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Nucleic Acids Res 41:e2. 2013
    ..Nevertheless, the method presented herein provides a rapid, accurate, highly-detailed and semi-quantitative analysis of RNA cleavage that should be widely applicable...
  4. pmc A structural model for glutathione-complexed iron-sulfur cluster as a substrate for ABCB7-type transporters
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, USA
    Chem Commun (Camb) 50:3795-8. 2014
    ..A possible substrate-binding site is identified on a new structural model for the active transporter. ..
  5. pmc Target-directed catalytic metallodrugs
    J C Joyner
    Evans Laboratory of Chemistry, Ohio State University, Columbus, OH 43210, USA
    Braz J Med Biol Res 46:465-85. 2013
    ....
  6. pmc Glutathione-complexed iron-sulfur clusters. Reaction intermediates and evidence for a template effect promoting assembly and stability
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 49:6313-5. 2013
    ..Studies of glutathione derivatives, in combination with mass spectrometric and Mössbauer investigations provide insight on reaction intermediates during formation of [2Fe-2S](GS)4(2-)...
  7. pmc Human ferredoxin-2 displays a unique conformational change
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA
    Dalton Trans 42:3088-91. 2013
    ..No such change was observed for hFd1, which may contribute to the distinct cellular functions of hFd1 and hFd2 under physiological conditions...
  8. pmc Antimicrobial metallopeptides with broad nuclease and ribonuclease activity
    Jeff C Joyner
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Chem Commun (Camb) 49:2118-20. 2013
    ....
  9. doi request reprint DNA nuclease activity of Rev-coupled transition metal chelates
    Jeff C Joyner
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    Dalton Trans 41:6567-78. 2012
    ..Moreover, the studies confirm high selectivity for the target HIV RRE RNA at the most likely dosage concentrations, lending further support to the feasibility of designing and applying targeted catalytic metallodrugs...
  10. doi request reprint Catalytic metallodrugs targeting HCV IRES RNA
    Seth Bradford
    Department of Chemistry, The Ohio State University, 100 W, 18th Ave, Columbus, OH 43210, USA
    Chem Commun (Camb) 48:3118-20. 2012
    ..85 μM, k(cat) of 0.53 min(-1), and a turnover number of 31.9 for Cu·GGHYrFK-amide (1-Cu), and yielded an antiviral activity (IC(50)) of 0.58 μM in an HCV cellular replicon assay...
  11. ncbi request reprint Chemical nucleases
    J A Cowan
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Curr Opin Chem Biol 5:634-42. 2001
    ..Finally, considerable progress has been made in identifying the molecular determinants for site-selective cleavage of RNA (in particular) and the design of ligands to achieve targeted recognition and cleavage...
  12. pmc Recognition of a cognate RNA aptamer by neomycin B: quantitative evaluation of hydrogen bonding and electrostatic interactions
    J A Cowan
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Nucleic Acids Res 28:2935-42. 2000
    ..The principal mechanism for recognition and binding of neomycin B to the RNA major groove is mediated by hydrogen bonding...
  13. ncbi request reprint Structural and catalytic chemistry of magnesium-dependent enzymes
    J A Cowan
    Evans Laboratory of Chemistry, The Ohio State University, Columbus 43210, USA
    Biometals 15:225-35. 2002
  14. ncbi request reprint Metal-ion stoichiometry of the HIV-1 RT ribonuclease H domain: evidence for two mutually exclusive sites leads to new mechanistic insights on metal-mediated hydrolysis in nucleic acid biochemistry
    J A Cowan
    Evans Laboratory of Chemistry, The Ohio State University, Columbus 43210, USA
    J Biol Inorg Chem 5:67-74. 2000
    ..The occurrence of distinct binding stoichiometries for Mg2+ and Mn2+ to multifunctional enzymes has previously been reported...
  15. doi request reprint Influence of charge and structure on the coordination chemistry of copper aminoglycosides
    Anjali Patwardhan
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Dalton Trans 40:1795-801. 2011
    ..A possible role for metal coordination in antibacterial activity is also considered...
  16. doi request reprint Mechanism of glutaredoxin-ISU [2Fe-2S] cluster exchange
    Wenbin Qi
    Ohio State Biochemistry Program, The Ohio State University, 100 West 18thAvenue, Columbus, OH 43210, USA
    Chem Commun (Camb) 47:4989-91. 2011
    ....
  17. doi request reprint Iron-sulfur cluster biosynthesis: characterization of IscU-IscS complex formation and a structural model for sulfide delivery to the [2Fe-2S] assembly site
    Manunya Nuth
    Evans Laboratory of Chemistry, Ohio State University, Columbus, OH 43210, USA
    J Biol Inorg Chem 14:829-39. 2009
    ..Both helix and loop regions are highly conserved among phylogenetically diverse organisms from a pool of archael, bacterial, fungal, and mammalian representatives...
  18. ncbi request reprint Frataxin-mediated iron delivery to ferrochelatase in the final step of heme biosynthesis
    Taejin Yoon
    Evans Laboratory of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:25943-6. 2004
    ....
  19. ncbi request reprint Characterization of the soluble domain of the ABC7 type transporter Atm1
    Chun An Chen
    Evans Laboratory of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 278:52681-8. 2003
    ..Accordingly, any cooperative response must be mediated through the transmembrane domain in the case of the eukaryotic Atm1 transporter...
  20. doi request reprint Catalytic inactivation of human carbonic anhydrase I by a metallopeptide-sulfonamide conjugate is mediated by oxidation of active site residues
    Nikhil H Gokhale
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    J Am Chem Soc 130:2388-9. 2008
  21. ncbi request reprint Iron-sulfur cluster biosynthesis: biochemical characterization of the conformational dynamics of Thermotoga maritima IscU and the relevance for cellular cluster assembly
    Sheref S Mansy
    Evans Laboratory of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:10469-75. 2004
    ..To describe such proteins, we introduce the term multiple discrete conformers...
  22. ncbi request reprint Catalytic hydrolysis of DNA by metal ions and complexes
    A Sreedhara
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Biol Inorg Chem 6:337-47. 2001
    ..Methods to evaluate their catalytic efficiencies are critically discussed, and a prognosis for future work in this area is presented...
  23. ncbi request reprint Factors influencing redox thermodynamics and electron self-exchange for the [Fe4S4] cluster in Chromatium vinosum high potential iron protein: the role of core aromatic residues in defining cluster redox chemistry
    A Soriano
    Evans Laboratory of Chemistry, Ohio State University, Columbus 43210, USA
    Biochemistry 35:12479-86. 1996
    ..Li, D., & Cowan, J.A. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 9440-9444], in which the aromatic core restricts solvent accessibility and thereby stabilizes the oxidized [Fe4S4]3+ cluster...
  24. ncbi request reprint Structural and catalytic roles for divalent magnesium in nucleic acid biochemistry
    Alavattam Sreedhara
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biometals 15:211-23. 2002
  25. ncbi request reprint Iron-sulfur cluster biosynthesis. Kinetic analysis of [2Fe-2S] cluster transfer from holo ISU to apo Fd: role of redox chemistry and a conserved aspartate
    Shu Pao Wu
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Biochemistry 41:8876-85. 2002
    ..Fully oxidized cluster appears to be the natural state for transfer to target proteins. Reduced Fd does not readily reduce ISU-bound [2Fe-2S](2+) and does not promote cluster transfer to an apo Fd target...
  26. ncbi request reprint Iron-sulfur cluster biosynthesis. A comparative kinetic analysis of native and Cys-substituted ISA-mediated [2Fe-2S]2+ cluster transfer to an apoferredoxin target
    Shu Pao Wu
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 42:5784-91. 2003
    ..et al. (2002) Biochemistry 41, 8876-8885], where the rates differed for Hs and Sp target Fd's, suggesting distinct binding sites for binding of holo ISA and ISU to apo Fd...
  27. pmc Iron-sulfur cluster biosynthesis: characterization of a molten globule domain in human NFU
    Yushi Liu
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 48:7512-8. 2009
    ....
  28. doi request reprint A structural and functional homolog supports a general role for frataxin in cellular iron chemistry
    Wenbin Qi
    Evans Laboratory of Chemistry, The Ohio State University, 100 West, 18th Avenue, Columbus, OH 43210, USA
    Chem Commun (Camb) 46:719-21. 2010
    ..Bacillus subtilis YdhG lacks sequence homology, but demonstrates structural and functional similarity to the frataxin family, supporting a general cellular role for frataxin-type proteins in cellular iron homeostasis...
  29. ncbi request reprint Iron-sulfur cluster biosynthesis. Thermatoga maritima IscU is a structured iron-sulfur cluster assembly protein
    Sheref S Mansy
    Evans Laboratory of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:21397-404. 2002
    ..Consistent with the observed sequence homology and proposed conservation of function for IscU-type proteins, we demonstrate T. maritima IscU-mediated reconstitution of human apoferredoxin...
  30. doi request reprint Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe-S cluster scaffold protein
    Jia Huang
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH, 43210, USA
    J Biol Inorg Chem 13:825-36. 2008
    ..The overall consensus from these data is to consider a pool of potential sites that can stably bind an iron center when bridged to a variety of physiological targets...
  31. doi request reprint Copper.Lys-Gly-His-Lys mediated cleavage of tRNA(Phe): studies of reaction mechanism and cleavage specificity
    Seth Bradford
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Inorg Biochem 103:871-5. 2009
    ..The data emphasizes the optimal positioning of the metal-associated reactive oxygen species, relative to scissile bonds, as a major criterion for development of efficient catalytic nucleases or therapeutics...
  32. ncbi request reprint Influence of stereochemistry and redox potentials on the single- and double-strand DNA cleavage efficiency of Cu(II) and Ni(II) Lys-Gly-His-derived ATCUN metallopeptides
    Yan Jin
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    J Am Chem Soc 129:8353-61. 2007
    ....
  33. ncbi request reprint Iron-sulfur cluster biosynthesis: characterization of iron nucleation sites for assembly of the [2Fe-2S]2+ cluster core in IscU proteins
    Manunya Nuth
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    J Am Chem Soc 124:8774-5. 2002
    ..The latter observation reflects the low binding affinity of persulfido IscU for iron ion...
  34. ncbi request reprint Iron-sulfur cluster biosynthesis. Characterization of frataxin as an iron donor for assembly of [2Fe-2S] clusters in ISU-type proteins
    Taejin Yoon
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus 43210, USA
    J Am Chem Soc 125:6078-84. 2003
    ..5) with 4.3 mM DTT. Similar rates were obtained for NifS-mediated sulfur delivery, consistent with iron release from frataxin as a rate-limiting step in the cluster assembly reaction...
  35. ncbi request reprint Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster
    Sheref S Mansy
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 41:1195-201. 2002
    ..Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation...
  36. pmc Characterization of a multi-functional metal-mediated nuclease by MALDI-TOF mass spectrometry
    U Puapaiboon
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Nucleic Acids Res 29:3652-6. 2001
    ..Several potential applications of these methods to quantitative studies of DNA repair chemistry are also described...
  37. ncbi request reprint Stimulation and oxidative catalytic inactivation of thermolysin by copper.Cys-Gly-His-Lys
    Nikhil H Gokhale
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Biol Inorg Chem 12:981-7. 2007
    ..7 +/- 0.9) x 10(4) M(-1) min(-1). Copper peptide mediated generation of reactive oxygen species that subsequently modify active-site residues is the most likely pathway for inactivation of TLN rather than cleavage of the peptide backbone...
  38. ncbi request reprint Targeted site-specific cleavage of HIV-1 viral Rev responsive element by copper aminoglycosides
    A Sreedhara
    Evans Laboratory of Chemistry, The Ohio State University, Columbus 43210, USA
    J Biol Inorg Chem 6:166-72. 2001
    ..These results demonstrate copper aminoglycosides to be highly efficient chemical nucleases with a targeting capability for viral RNA and suggest a novel methodology to counter RNA viruses...
  39. ncbi request reprint Metallopeptide-promoted inactivation of angiotensin-converting enzyme and endothelin-converting enzyme 1: Toward dual-action therapeutics
    Nikhil H Gokhale
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Biol Inorg Chem 11:937-47. 2006
    ..Irreversible inactivation of a drug target represents a novel mechanism of drug action that complements existing classical inhibitor strategies that underlie current drug discovery efforts...
  40. ncbi request reprint N-terminal iron-mediated self-cleavage of human frataxin: regulation of iron binding and complex formation with target proteins
    Taejin Yoon
    Evans Laboratory of Chemistry, Ohio State University, Columbus, OH 43210, USA
    J Biol Inorg Chem 12:535-42. 2007
    ..The physiological significance of iron-mediated release of the N-terminal residues from this anionic surface is discussed...
  41. ncbi request reprint Synthesis, cloning and expression of a synthetic gene for high potential iron protein from Chromatium vinosum
    A Agarwal
    Evans Laboratory of Chemistry, Ohio State University, Columbus 43210
    Biochem Biophys Res Commun 197:1357-62. 1993
    ..This is the first example of the expression of a high potential ferredoxin containing a fully constituted [Fe4S4] cluster...
  42. ncbi request reprint 15N resonance assignments of oxidized and reduced Chromatium vinosum high-potential iron protein
    D Li
    Evans Laboratory of Chemistry, Ohio State University, Columbus 43210, USA
    J Protein Chem 14:115-26. 1995
    ..HMQC-COSY, and HMQC-NOESY spectra. Unambiguous assignment of 70 of 85 backbone 15N resonances in the reduced protein and 62 of 85 resonances in the oxidized protein are made, as are 12 of 21 side-chain 15N resonances...
  43. ncbi request reprint Primary structure of the assimilatory-type sulfite reductase from Desulfovibrio vulgaris (Hildenborough): cloning and nucleotide sequence of the reductase gene
    J Tan
    Evans Laboratory of Chemistry, Ohio State University, Columbus 43210
    Biochemistry 30:9900-7. 1991
    ..Lina, A. R., Moura, J. J. G., Xavier, A. V., Fauque, G., Peck, H. D., & Le Gall, J. (1986) Biochem. Biophys. Res. Commun. 141, 1032-1041]...
  44. ncbi request reprint DNA cleavage by copper-ATCUN complexes. Factors influencing cleavage mechanism and linearization of dsDNA
    Yan Jin
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Am Chem Soc 127:8408-15. 2005
    ..Linearization of DNA was observed for [KGHK-Cu](+) relative to [GGH-Cu](-), consistent with the increased positive charge and longer residency time on dsDNA...
  45. ncbi request reprint Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe
    Gong Wu
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 41:5024-32. 2002
    ..Cross-linking of holo ISU1 with holo Fd is consistent with a role for redox chemistry in cluster assembly and may mimic the intramolecular complex already defined in NifU...
  46. ncbi request reprint 1H NMR studies of oxidized high-potential iron protein from Chromatium vinosum. Nuclear Overhauser effect measurements
    J A Cowan
    Evans Laboratory of Chemistry, Ohio State University, Columbus 43210
    Biochemistry 29:5633-7. 1990
    ..A partial assignment to individual residues has been proposed from a computer graphics analysis of the X-ray structure. Tentative assignments of other resonances are discussed...
  47. ncbi request reprint Iron-sulfur cluster biosynthesis: toward an understanding of cellular machinery and molecular mechanism
    Sheref S Mansy
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Acc Chem Res 37:719-25. 2004
    ..IscU in particular exhibits highly unusual conformational flexibility that appears critical to its function...
  48. ncbi request reprint Iron-sulfur cluster biosynthesis. Molecular chaperone DnaK promotes IscU-bound [2Fe-2S] cluster stability and inhibits cluster transfer activity
    Shu Pao Wu
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 44:4284-93. 2005
    ..In accord with prior observations the cochaperone DnaJ stimulates the ATPase activity of DnaK, but has a minimal influence on IscU cluster transfer activity, either alone or in concert with DnaK...
  49. ncbi request reprint Targeted cleavage of HIV rev response element RNA by metallopeptide complexes
    Yan Jin
    Evans Laboratory of Chemistry, The Ohio State University, Columbus, 43210, USA
    J Am Chem Soc 128:410-1. 2006
    ....
  50. doi request reprint Iron-sulfur cluster biosynthesis: functional characterization of the N- and C-terminal domains of human NFU
    Yushi Liu
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 48:973-80. 2009
    ..This is consistent with electrostatic potential maps for bacterial homologues of the N- and C-terminal domains of human NFU, which most likely reflect the structural characteristics expected for full-length human NFU...
  51. ncbi request reprint Cellular activity of Rev response element RNA targeting metallopeptides
    Yan Jin
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Biol Inorg Chem 12:637-44. 2007
    ..The RRE RNA cleavage activities of metallopeptide complexes were further confirmed by in vitro experiments and mammalian cell assays...
  52. ncbi request reprint Magnesium chemistry and biochemistry
    Michael E Maguire
    Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106 4965, USA
    Biometals 15:203-10. 2002

Research Grants2

  1. Novel Inorganic Nucleases
    James Cowan; Fiscal Year: 2006
    ..abstract_text> ..
  2. Novel Engineered Synthetic Metallodrugs Directed Against HCV
    San Ching; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..