Affiliation: The Children's Hospital of Philadelphia
- Functional regulation of the dentate gyrus by GABA-mediated inhibitionDouglas A Coulter
The Children s Hospital of Philadelphia, Abramson Pediatric Research Center, Philadelphia, PA 19104 4318, USA
Prog Brain Res 163:235-43. 2007..Both synaptic and extrasynaptic GABA(A) receptors exhibit profound dysregulation in animal models of temporal lobe epilepsy, which may contribute to the hippocampal hyperexcitability that defines this disorder...
- Selective induction of astrocytic gliosis generates deficits in neuronal inhibitionPavel I Ortinski
Division of Neurology, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Nat Neurosci 13:584-91. 2010..Thus, reactive astrocytosis could generate local synaptic perturbations, leading to broader functional deficits associated with neurologic disease...
- Hippocampal microcircuit dynamics probed using optical imaging approachesDouglas A Coulter
Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
J Physiol 589:1893-903. 2011....
- Animal models of limbic epilepsies: what can they tell us?Douglas A Coulter
Department of Neuroscience, University of Pennsylvania School of Medicine, Children s Hospital of Philadelphia, 19104 4318, USA
Brain Pathol 12:240-56. 2002..We have also provided a brief introduction into how animal models of temporal lobe epilepsy facilitate use of modern state-of-the-art techniques in neurobiology to address critical questions in the pathogenesis of epilepsy...
- Massive and specific dysregulation of direct cortical input to the hippocampus in temporal lobe epilepsyChyze W Ang
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Neurosci 26:11850-6. 2006..This dysregulated temporoammonic pathway is critically positioned to mediate generation and/or propagation of seizure activity in the hippocampus...
- Deficits in phosphorylation of GABA(A) receptors by intimately associated protein kinase C activity underlie compromised synaptic inhibition during status epilepticusMiho Terunuma
Department of Neuroscience, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Neurosci 28:376-84. 2008..Thus, enhancing phosphorylation of GABA(A)Rs or selectively blocking their interaction with AP2 may provide novel therapeutic strategies to ameliorate SE...
- Dentate granule cell GABA(A) receptors in epileptic hippocampus: enhanced synaptic efficacy and altered pharmacologyAkiva S Cohen
Pediatric Regional Epilepsy Program, Pennsylvania, USA
Eur J Neurosci 17:1607-16. 2003....
- Heterogeneous GABAA receptor subunit expression in pediatric epilepsy patientsBrenda E Porter
Division of Neurology, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
Neurobiol Dis 18:484-91. 2005..Patient-specific GABAAR expression might contribute to variability in anti-epileptic drug efficacy, side-effect profiles, and seizure susceptibility...
- Disrupted dentate granule cell chloride regulation enhances synaptic excitability during development of temporal lobe epilepsyHemal R Pathak
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Neurosci 27:14012-22. 2007..This pathophysiological process may constitute a significant mechanism linking injury to the subsequent development of epilepsy...
- Increased expression of the neuronal glutamate transporter (EAAT3/EAAC1) in hippocampal and neocortical epilepsyPeter B Crino
PENN Epilepsy Center, Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
Epilepsia 43:211-8. 2002..To define the changes in gene and protein expression of the neuronal glutamate transporter (EAAT3/EAAC1) in a rat model of temporal lobe epilepsy as well as in human hippocampal and neocortical epilepsy...
- Shift of intracellular chloride concentration in ganglion and amacrine cells of developing mouse retinaLing Li Zhang
Deaprtment of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
J Neurophysiol 95:2404-16. 2006..Our results support the hypothesis that a shift from high [Cl-]i to low causes GABA to switch from excitatory to inhibitory...
- Axonal α7 nicotinic ACh receptors modulate presynaptic NMDA receptor expression and structural plasticity of glutamatergic presynaptic boutonsHong Lin
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 107:16661-6. 2010....
- Developmental and cell-selective variations in N-methyl-D-aspartate receptor degradation by calpainYi Na Dong
Department of Neurology and Pediatrics, University of Pennsylvania and The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 4318, USA
J Neurochem 99:206-17. 2006..Our data suggest that the susceptibility of the NMDA receptor to cleavage by calpain varies with neuronal maturity in a manner that may alter its electrophysiological properties...
- Enhanced astrocytic Ca2+ signals contribute to neuronal excitotoxicity after status epilepticusShinghua Ding
Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Neurosci 27:10674-84. 2007..These observations support neurotoxic roles for astrocytic gliotransmission in pathological conditions and identify this process as a novel therapeutic target...
- In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recordingGreg C Carlson
Translational Research Laboratory, University of Pennsylvania School of Medicine, Room 2226, 125 S 31st Street, Philadelphia, Pennsylvania 19104 3403, USA
Nat Protoc 3:249-55. 2008..Thus, the combined VSD imaging and whole-cell patch approach provides experimental resolution spanning single-cell electrophysiology to complex local circuit responses...
- AMPA glutamate receptor-mediated calcium signaling is transiently enhanced during development of oligodendrocytesTakayuki Itoh
The Children s Hospital of Philadelphia, Pennsylvania, USA
J Neurochem 81:390-402. 2002..susceptibility to excitotoxicity of cells at these stages of oligodendroglial differentiation, and is likely to be important to these cells in the trans-synaptic Ca2+-signaling from glutamatergic neurons, which occurs in hippocampus..
- Repeated neonatal handling with maternal separation permanently alters hippocampal GABAA receptors and behavioral stress responsesFu Chun Hsu
The Children s Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 100:12213-8. 2003....
- Hippocampal CA1 circuitry dynamically gates direct cortical inputs preferentially at theta frequenciesChyze W Ang
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Neurosci 25:9567-80. 2005....
- Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systemsHai Yan Wu
Departments of Pediatrics and Neurology, University of Pennsylvania, Philadelphia, PA 19104, USA
J Biol Chem 282:20075-87. 2007..Thus, the Fyn-controlled regulation of NMDA receptor cleavage by calpain may play critical roles in controlling NMDA receptor properties during synaptic plasticity and excitotoxicity...
- The incorporation of an ion channel gene mutation associated with the long QT syndrome (Q9E-hMiRP1) in a plasmid vector for site-specific arrhythmia gene therapy: in vitro and in vivo feasibility studiesDenise Y Burton
Division of Cardiology, Children s Hospital of Philadelphia, Civic Center Boulevard, Philadelphia, PA 19104 4318, USA
Hum Gene Ther 14:907-22. 2003....
- Dynamic regulation of synaptic GABA release by the glutamate-glutamine cycle in hippocampal area CA1Shu Ling Liang
Division of Neurology and the Pediatric Regional Epilepsy Program, Children s Hospital of Philadelphia, Pennsylvania 19104, USA
J Neurosci 26:8537-48. 2006..We conclude that the glutamate-glutamine cycle is a major contributor to synaptic GABA release under physiological conditions, which dynamically regulates inhibitory synaptic strength...
- A neuronal glutamate transporter contributes to neurotransmitter GABA synthesis and epilepsyJehuda P Sepkuty
Department of Neurology, Johns Hopkins University, Baltimore, Maryland 21287 7247, USA
J Neurosci 22:6372-9. 2002..EAAC1 may participate in normal GABA neurosynthesis and limbic hyperexcitability, whereas epilepsy can result from a disruption of the interaction between EAAC1 and GABA metabolism...
- Induction of prolonged electrographic seizures in vitro has a defined threshold and is all or none: implications for diagnosis of status epilepticusAzhar Rafiq
Department of Neurology and the VCU Comprehensive Epilepsy Center of Virginia Commonwealth University, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298 0599, USA
Epilepsia 44:1034-41. 2003..To study whether induction of prolonged (>30-min duration) in vitro electrographic seizure discharges resembling status epilepticus (SE) is graded or all-or-none, and to determine the critical factors mediating SE induction...
- Anticonvulsant actions of lamotrigine on spontaneous thalamocortical rhythmsJohn W Gibbs
Department of Anatomy, Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia, USA
Epilepsia 43:342-9. 2002....
- Clioquinol effects on tissue chelatable zinc in miceYuval B Nitzan
Department of Morphology, Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
J Mol Med (Berl) 81:637-44. 2003..Thus, our findings indicate that CQ has considerable potential as a versatile scientific and clinical tool used for selective modulation of zinc pools...
- Models for epilepsy and epileptogenesis: report from the NIH workshop, Bethesda, MarylandJames P Stables
National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA
Epilepsia 43:1410-20. 2002..To move toward new and more effective therapies, novel approaches to therapy discovery are needed...
- PHYSIOLOGY OF THALAMOCORTICAL RHYTHMICITY IN VITRODOUGLAS COULTER; Fiscal Year: 2001....
- Transcriptional Repression as a Therapeutic Target in EpileptogenesisDOUGLAS COULTER; Fiscal Year: 2006..abstract_text> ..
- PHYSIOLOGY OF STATUS EPILEPTICUS IN VITRODOUGLAS COULTER; Fiscal Year: 2008..abstract_text> ..
- 2008 Mechanisms of Epilepsy and Neuronal Synchronization GRCDOUGLAS COULTER; Fiscal Year: 2008..This should significantly advance research directed towards better treatment of epilepsy. [unreadable] [unreadable] [unreadable]..