Affiliation: The Rockefeller University
- CTLA-4: From conflict to clinicHema Bashyam
J Exp Med 204:1243. 2007..CTLA-4 has since scored in the clinic as a target for antitumor therapy and as a soluble inhibitor of autoimmunity...
- Sabine Ehrt: searching for mycobacterial stress points. Interview by Hema BashyamSabine Ehrt
J Exp Med 205:2184-5. 2008
- Ralph Steinman: dendritic cells bring home the LaskerHema Bashyam
J Exp Med 204:2245-8. 2007..Yet the man behind the research holds his praise for the many other scientists-in the U.S. and abroad-who have further advanced the therapeutic promise of DCs...
- Susan Allen: confronting HIV in Africa. Interview by Hema BashyamSusan Allen
J Exp Med 205:1000-1. 2008
- Vamsi Mootha: taking an inventory of mitochondria. Interview by Hema BashyamVamsi Mootha
J Exp Med 205:1248-9. 2008
- Caetano Reis e Sousa: harnessing DC power. Interview by Hema BashyamCaetano Reis E Sousa
J Exp Med 205:1946-7. 2008
- Facundo Batista: watching B cells spread and grab antigens. Interview by Hema BashyamFacundo Batista
J Exp Med 205:1718-9. 2008
- Jane Skok: choreography of allelic exclusion. [Interview by Hema Bashyam]Jane Skok
J Exp Med 205:1514-5. 2008
- Uncovering the dark side of TNFHema Bashyam
J Exp Med 204:2013. 2007..They soon found out that their malevolent cytokine already had another name-tumor necrosis factor (TNF)-and was being lauded as a potential antitumor agent...
- Michael T. McManus: Interrupting biology [interview by Hema Bashyam]Michael T McManus
J Exp Med 205:506-7. 2008
- Receptor editing: how B cells stay tolerantHema Bashyam
J Exp Med 204:1735. 2007..This process, called "receptor editing," has since gained prominence as the main mechanism of B cell tolerance...
- Lewis Dahl and the genetics of salt-induced hypertensionHema Bashyam
J Exp Med 204:1507. 2007
- Robert Menard: Tailing malaria parasites to the red blood cell. Interviewed by Hema BashyamHema Bashyam
J Exp Med 205:744-5. 2008
- Anthony De Tomaso: Finding the origins of adaptive immunity. Interview by Hema BashyamAnthony De Tomaso
J Exp Med 205:264-5. 2008
- Crossing barriers in transplantationHema Bashyam
J Exp Med 204:459. 2007..T cell depletion of donor grafts has since become a staple of transplantation strategies to combat leukemia and other inherited blood disorders...
- Th1/Th2 cross-regulation and the discovery of IL-10Hema Bashyam
J Exp Med 204:237. 2007..Knowledge of the inhibition led to the discovery that Th2 cells make IL-10 to suppress Th1 cells...
- IFNgamma: issuing macrophages a license to killHema Bashyam
J Exp Med 204:3. 2007..In 1983, Carl Nathan proved that this start signal comes in the form of the secreted cytokine IFNgamma...
- Essential role for Dicer during skeletal muscle developmentJason R O'Rourke
Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
Dev Biol 311:359-68. 2007..These observations demonstrate key roles for Dicer in skeletal muscle and implicate miRNAs as critical components required for embryonic myogenesis...
- A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interferenceDouglas A Rubinson
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Genet 33:401-6. 2003..They may also provide new approaches for gene therapy...
- Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunityXuyu Zhou
Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA
J Exp Med 205:1983-91. 2008..These results support a central role for miRNAs in maintaining the stability of differentiated T reg cell function in vivo and homeostasis of the adaptive immune system...
- Small interfering RNA-mediated gene silencing in T lymphocytesMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, MA 02139, USA
J Immunol 169:5754-60. 2002..Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes...
- Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular diseaseScott J Harvey
Renal Division, Washington University School of Medicine, St Louis, MO 63110, USA
J Am Soc Nephrol 19:2150-8. 2008..The unique architecture of podocytes may make them especially susceptible to cytoskeletal alterations initiated by aberrant miRNA dynamics...
- RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcriptionQing Ge
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 100:2718-23. 2003..The findings reveal that newly synthesized NP and PA proteins are required for influenza virus transcription and replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection in humans...
- MicroRNAs and cancerMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA
Semin Cancer Biol 13:253-8. 2003..Recent data suggests that a special class of ncRNAs called microRNAs might be involved in human disease. This review proposes a role for microRNAs in oncogenesis...
- MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expressionJennifer H Mansfield
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 36:1079-83. 2004..Furthermore, miR-196a negatively regulates Hoxb8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex...
- Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampusTigwa H Davis
Department of Ophthalmology, University of California, San Francisco, San Francisco, California 94143 0730, USA
J Neurosci 28:4322-30. 2008....
- Lentiviral strategies for RNAi knockdown of neuronal genesJill R Crittenden
Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Curr Protoc Neurosci . 2007..Additional protocols describe production and titering of the lentivirus, as well as safety testing. Finally, methods are provided for infecting neurons in culture and in vivo with RNAi lentivirus...
- The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limbBrian D Harfe
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
Proc Natl Acad Sci U S A 102:10898-903. 2005..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
- Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegenerationTrinna L Cuellar
Department of Microbiology and Immunology, Diabetes Center, Biomedical Sciences Graduate Program, and Departments of Ophthalmology and Physiology, University of California, San Francisco, CA 94122 0534, USA
Proc Natl Acad Sci U S A 105:5614-9. 2008..These studies highlight the complicated nature of Dicer ablation in the brain and provide a useful mouse model for studying dopaminoceptive neuron function...
- Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2Yong Zhao
Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
Cell 129:303-17. 2007..These findings indicate that subtle alteration of miRNA dosage can have profound consequences in mammals and demonstrate the utility of mammalian loss-of-function models in revealing physiologic miRNA targets...
- MicroRNA expression is required for pancreatic islet cell genesis in the mouseFrancis C Lynn
Diabetes Center, Hormone Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143 0534, USA
Diabetes 56:2938-45. 2007..We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development...
- Dicer function is essential for lung epithelium morphogenesisKelley S Harris
Laboratory of Genetics, University of Wisconsin, 425 G Henry Mall, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 103:2208-13. 2006..The aberrant Fgf10 expression may contribute to the Dicer morphological defects. However, the mechanism by which DICER functions in the epithelium to influence Fgf10 expression in the mesenchyme remains unknown...
- MicroRNAs and endocrine biologyTrinna L Cuellar
UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, California 94122 0534, USA
J Endocrinol 187:327-32. 2005..This review provides a general background regarding current knowledge about miRNA biogenesis and the potential contributions of these RNAs to endocrine function...
- Behind the scenes of a small RNA gene-silencing pathwayGregory Ku
Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Francisco, San Francisco, CA 94122, USA
Hum Gene Ther 19:17-26. 2008..This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA-based treatments...
- Unintentional miRNA ablation is a risk factor in gene knockout studies: a short reportIvan Osokine
Department of Microbiology and Immunology, University of California San Francisco Diabetes Center, University of California San Francisco, San Francisco, California, United States of America
PLoS Genet 4:e34. 2008..These data also raise the possibility that many knockout studies may need to be reexamined to determine if loss of a microRNA contributes to the phenotypic consequences attributed to loss of a protein-encoding gene...
- The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb developmentEran Hornstein
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
Nature 438:671-4. 2005....
- Slowing down the Ras lane: miRNAs as tumor suppressors?John P Morris
UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 0534, USA
Sci STKE 2005:pe41. 2005....
- Gene silencing in mammals by small interfering RNAsMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, Massachusetts 02139, USA
Nat Rev Genet 3:737-47. 2002..Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology...