Arthur E Johnson

Summary

Affiliation: Texas A and M University
Country: USA

Publications

  1. ncbi Maintaining the permeability barrier during protein trafficking at the endoplasmic reticulum membrane
    A E Johnson
    Department of Medical Biochemistry and Genetics, College of Medicine, 116 Reynolds Medical Building, Texas A and M University System Health Science Center, 1114 TAMU, College Station, TX 77843 1114, U S A
    Biochem Soc Trans 31:1227-31. 2003
  2. pmc The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum
    Nathan N Alder
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 168:389-99. 2005
  3. pmc A new role for BiP: closing the aqueous translocon pore during protein integration into the ER membrane
    Nora G Haigh
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    J Cell Biol 156:261-70. 2002
  4. pmc Photocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex
    Ingmarie Nilsson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 161:715-25. 2003
  5. ncbi The location of the active site of blood coagulation factor VIIa above the membrane surface and its reorientation upon association with tissue factor. A fluorescence energy transfer study
    C D McCallum
    Department of Medical Biochemistry and Genetics, Texas A and M University Health Science Center, College Station, Texas 77843 1114, USA
    J Biol Chem 271:28168-75. 1996
  6. ncbi Functional ramifications of FRET-detected nascent chain folding far inside the membrane-bound ribosome
    A E Johnson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, 116 Reynolds Medical Bldg, 1114 TAMU, College Station, TX 77843 1114, USA
    Biochem Soc Trans 32:668-72. 2004
  7. ncbi The co-translational folding and interactions of nascent protein chains: a new approach using fluorescence resonance energy transfer
    Arthur E Johnson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    FEBS Lett 579:916-20. 2005
  8. ncbi Tissue factor positions and maintains the factor VIIa active site far above the membrane surface even in the absence of the factor VIIa Gla domain. A fluorescence resonance energy transfer study
    C D McCallum
    Department of Medical Biochemistry and Genetics, Texas A and M University, College Station, Texas 77843, USA
    J Biol Chem 272:30160-6. 1997
  9. ncbi The translocon: a dynamic gateway at the ER membrane
    A E Johnson
    Department of Medical Biochemistry, Texas A and M University, College Station 77843, USA
    Annu Rev Cell Dev Biol 15:799-842. 1999
  10. ncbi Cotranslational protein integration into the ER membrane is mediated by the binding of nascent chains to translocon proteins
    Peter J McCormick
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Mol Cell 12:329-41. 2003

Collaborators

Detail Information

Publications58

  1. ncbi Maintaining the permeability barrier during protein trafficking at the endoplasmic reticulum membrane
    A E Johnson
    Department of Medical Biochemistry and Genetics, College of Medicine, 116 Reynolds Medical Building, Texas A and M University System Health Science Center, 1114 TAMU, College Station, TX 77843 1114, U S A
    Biochem Soc Trans 31:1227-31. 2003
    ..These mechanisms are both simple and direct in concept, but are operationally complex and require the co-ordinated and regulated interaction of several multicomponent complexes...
  2. pmc The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum
    Nathan N Alder
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 168:389-99. 2005
    ..The mechanism by which BiP mediates translocon pore closure and opening is therefore similar to that in which Hsp70 chaperones associate with and dissociate from substrates...
  3. pmc A new role for BiP: closing the aqueous translocon pore during protein integration into the ER membrane
    Nora G Haigh
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    J Cell Biol 156:261-70. 2002
    ..Therefore, closure of the pore involves substantial conformational changes in the translocon that are coupled to a complex sequence of structural rearrangements on both sides of the ER membrane involving the ribosome and BiP...
  4. pmc Photocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex
    Ingmarie Nilsson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    J Cell Biol 161:715-25. 2003
    ....
  5. ncbi The location of the active site of blood coagulation factor VIIa above the membrane surface and its reorientation upon association with tissue factor. A fluorescence energy transfer study
    C D McCallum
    Department of Medical Biochemistry and Genetics, Texas A and M University Health Science Center, College Station, Texas 77843 1114, USA
    J Biol Chem 271:28168-75. 1996
    ..This cofactor-dependent realignment of the active-site groove presumably facilitates and optimizes fVIIa cleavage of its membrane-bound substrates...
  6. ncbi Functional ramifications of FRET-detected nascent chain folding far inside the membrane-bound ribosome
    A E Johnson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, 116 Reynolds Medical Bldg, 1114 TAMU, College Station, TX 77843 1114, USA
    Biochem Soc Trans 32:668-72. 2004
    ....
  7. ncbi The co-translational folding and interactions of nascent protein chains: a new approach using fluorescence resonance energy transfer
    Arthur E Johnson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    FEBS Lett 579:916-20. 2005
    ....
  8. ncbi Tissue factor positions and maintains the factor VIIa active site far above the membrane surface even in the absence of the factor VIIa Gla domain. A fluorescence resonance energy transfer study
    C D McCallum
    Department of Medical Biochemistry and Genetics, Texas A and M University, College Station, Texas 77843, USA
    J Biol Chem 272:30160-6. 1997
    ..TF complex. The extracellular domain of tissue factor therefore must be fairly rigid and fixed relative to the surface to position and maintain the fVIIa active site far above the membrane even in the absence of the fVIIa Gla domain...
  9. ncbi The translocon: a dynamic gateway at the ER membrane
    A E Johnson
    Department of Medical Biochemistry, Texas A and M University, College Station 77843, USA
    Annu Rev Cell Dev Biol 15:799-842. 1999
    ....
  10. ncbi Cotranslational protein integration into the ER membrane is mediated by the binding of nascent chains to translocon proteins
    Peter J McCormick
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Mol Cell 12:329-41. 2003
    ....
  11. ncbi Membrane-dependent conformational changes initiate cholesterol-dependent cytolysin oligomerization and intersubunit beta-strand alignment
    Rajesh Ramachandran
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843, USA
    Nat Struct Mol Biol 11:697-705. 2004
    ..Collectively, these data reveal a novel membrane binding-dependent mechanism for regulating cytolysin monomer-monomer association and pore formation...
  12. ncbi Mapping the interaction of the STT3 subunit of the oligosaccharyl transferase complex with nascent polypeptide chains
    Andrey L Karamyshev
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, USA
    J Biol Chem 280:40489-93. 2005
    ..Our results show that the addition of a glycan moiety in a stretch of approximately 15 residues surrounding a QK(*)T cross-linking site impairs the interaction between the nascent chain and STT3...
  13. ncbi The cotranslational contacts between ribosome-bound nascent polypeptides and the subunits of the hetero-oligomeric chaperonin TRiC probed by photocross-linking
    Stephanie A Etchells
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843, USA
    J Biol Chem 280:28118-26. 2005
    ..We conclude that elongating actin and luciferase nascent chains contact multiple TRiC subunits upon emerging from the ribosome, and that the TRiC subunits contacted by nascent actin change as it elongates and starts to fold...
  14. pmc Quaternary structure of the mitochondrial TIM23 complex reveals dynamic association between Tim23p and other subunits
    Nathan N Alder
    Department of Molecular and Cellular Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    Mol Biol Cell 19:159-70. 2008
    ..These structural changes reveal that the macromolecular arrangement within the TIM23 complex is dynamic and varies with the physiological state of the mitochondrion...
  15. pmc Real-time fluorescence detection of ERAD substrate retrotranslocation in a mammalian in vitro system
    Judit Wahlman
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Cell 129:943-55. 2007
    ..In addition, pro-alpha factor photocrosslinked derlin-1, but not Sec61alpha. Thus, derlin-1 appears to be involved in pro-alpha factor retrotranslocation...
  16. pmc Disulfide-bond scanning reveals assembly state and β-strand tilt angle of the PFO β-barrel
    Takehiro K Sato
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, Texas, USA
    Nat Chem Biol 9:383-9. 2013
    ..The PFO PPC-to-pore transition therefore converts TMHs in a dynamic folding intermediate far above the membrane into TMHs that are hydrogen bonded to those of adjacent subunits in the bilayer-embedded β-barrel...
  17. doi Fluorescence mapping of mitochondrial TIM23 complex reveals a water-facing, substrate-interacting helix surface
    Nathan N Alder
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843 1114, USA
    Cell 134:439-50. 2008
    ..This approach has therefore provided an unprecedented view of a translocon channel structure in an intact, fully operational, membrane-embedded complex...
  18. ncbi Signal recognition particle binds to ribosome-bound signal sequences with fluorescence-detected subnanomolar affinity that does not diminish as the nascent chain lengthens
    John J Flanagan
    Department of Medical Biochemistry and Genetics, Health Science Center, Texas A and M University, College Station, Texas 77843, USA
    J Biol Chem 278:18628-37. 2003
    ..RNC association with the SRP receptor and/or translocon...
  19. pmc Ricin A chain insertion into endoplasmic reticulum membranes is triggered by a temperature increase to 37 {degrees}C
    Peter U Mayerhofer
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, Texas 77843 1114, USA
    J Biol Chem 284:10232-42. 2009
    ..The sharp temperature dependence of these changes in RTA further suggests that they occur optimally in mammalian targets of the plant toxin...
  20. pmc Membrane protein TM segments are retained at the translocon during integration until the nascent chain cues FRET-detected release into bulk lipid
    Bo Hou
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843 1114, USA
    Mol Cell 48:398-408. 2012
    ....
  21. pmc The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import
    Alison J Davis
    Department of Molecular and Cellular Medicine, Texas A and M University System Health Science Center, College Station, TX 77843 1114, USA
    Mol Biol Cell 18:475-86. 2007
    ....
  22. pmc Transmembrane segments of nascent polytopic membrane proteins control cytosol/ER targeting during membrane integration
    Pen Jen Lin
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843, USA
    J Cell Biol 195:41-54. 2011
    ..Importantly, the TMS-triggered structural rearrangements that cycle nascent chain exposure between cytosolic and lumenal occur without compromising the permeability barrier of the ER membrane...
  23. pmc The domains of a cholesterol-dependent cytolysin undergo a major FRET-detected rearrangement during pore formation
    Rajesh Ramachandran
    Department of Biochemistry, Texas A and M University, College Station, TX 77843 1114, USA
    Proc Natl Acad Sci U S A 102:7139-44. 2005
    ..Therefore, the extended D2 beta-structure that connects D1 to membrane-bound D4 appears to bend or otherwise reconfigure during the prepore-to-pore transition of the perfringolysin O oligomer...
  24. ncbi Evidence for the "dock, lock, and latch" ligand binding mechanism of the staphylococcal microbial surface component recognizing adhesive matrix molecules (MSCRAMM) SdrG
    M Gabriela Bowden
    Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, 2121 W Holcombe Boulevard, Houston, TX 77030, USA
    J Biol Chem 283:638-47. 2008
    ....
  25. ncbi Assembly and topography of the prepore complex in cholesterol-dependent cytolysins
    Alejandro P Heuck
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, Texas 77843 1114, USA
    J Biol Chem 278:31218-25. 2003
    ....
  26. ncbi Nascent membrane and secretory proteins differ in FRET-detected folding far inside the ribosome and in their exposure to ribosomal proteins
    Cheryl A Woolhead
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    Cell 116:725-36. 2004
    ....
  27. ncbi Selective SecA association with signal sequences in ribosome-bound nascent chains: a potential role for SecA in ribosome targeting to the bacterial membrane
    Andrey L Karamyshev
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, 77843 1114, USA
    J Biol Chem 280:37930-40. 2005
    ....
  28. ncbi Importin-alpha-16 is a translocon-associated protein involved in sorting membrane proteins to the nuclear envelope
    Suraj Saksena
    Department of Biochemistry and Biophysics, Texas A and M University, USA
    Nat Struct Mol Biol 13:500-8. 2006
    ..Thus, it seems that during and after cotranslational membrane integration, importin-alpha-16 is involved in the trafficking of integral membrane proteins to the INM...
  29. pmc Cholesterol exposure at the membrane surface is necessary and sufficient to trigger perfringolysin O binding
    John J Flanagan
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843, USA
    Biochemistry 48:3977-87. 2009
    ....
  30. pmc The structural and functional coupling of two molecular machines, the ribosome and the translocon
    Arthur E Johnson
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center and Department of Chemistry and Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    J Cell Biol 185:765-7. 2009
    ..The study also identifies an unexpected component in the apparent molecular linkage that connects the two machines, a discovery that shows the current view of translocon structure is oversimplified...
  31. pmc Cotranslational integration and initial sorting at the endoplasmic reticulum translocon of proteins destined for the inner nuclear membrane
    Suraj Saksena
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Proc Natl Acad Sci U S A 101:12537-42. 2004
    ..Taken together, these data indicate that membrane protein sorting to the INM is an active process involving specific nonnuclear proteins...
  32. pmc Polytopic membrane protein folding at L17 in the ribosome tunnel initiates cyclical changes at the translocon
    Pen Jen Lin
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, College Station, TX 77843, USA
    J Cell Biol 195:55-70. 2011
    ..Thus, cyclical changes at the membrane during integration are initiated by TMS folding, even though nascent chain conformation and location vary dynamically in the ribosome tunnel. Nascent chains therefore control their own trafficking...
  33. ncbi Cotranslational membrane protein biogenesis at the endoplasmic reticulum
    Nathan N Alder
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, Texas 77843 1114, USA
    J Biol Chem 279:22787-90. 2004
  34. pmc Arginine changes the conformation of the arginine attenuator peptide relative to the ribosome tunnel
    Cheng Wu
    Department of Biology, Texas A and M University, College Station, TX 77843, USA
    J Mol Biol 416:518-33. 2012
    ..These data provide evidence that an Arg-induced change in AAP conformation and/or environment in the ribosome tunnel is important for stalling...
  35. ncbi Fluorescence approaches for determining protein conformations, interactions and mechanisms at membranes
    Arthur E Johnson
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station, TX 77843, USA
    Traffic 6:1078-92. 2005
    ..This article describes the use of various fluorescence techniques to characterize different aspects of proteins bound to or embedded in membranes...
  36. ncbi Structural insights into the membrane-anchoring mechanism of a cholesterol-dependent cytolysin
    Rajesh Ramachandran
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843, USA
    Nat Struct Biol 9:823-7. 2002
    ..Furthermore, D4 serves as the fulcrum around which extensive structural changes occur during the formation and insertion of the large transmembrane beta-barrel into the bilayer...
  37. ncbi N-linked glycans direct the cotranslational folding pathway of influenza hemagglutinin
    Robert Daniels
    Department of Biochemistry and Molecular Biology, Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Mol Cell 11:79-90. 2003
    ....
  38. ncbi Pore-forming protein structure analysis in membranes using multiple independent fluorescence techniques
    Alejandro P Heuck
    Department of Medical Biochemistry and Genetics, Texas A and M University System Health Science Center, College Station 77843 1114, USA
    Cell Biochem Biophys 36:89-101. 2002
    ..This review briefly outlines how MIFT can reveal the identity, location, conformation, and topography of the polypeptide sequences that interact with the membrane...
  39. pmc Control of translocation through the Sec61 translocon by nascent polypeptide structure within the ribosome
    Colin J Daniel
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, OR 97239, USA
    J Biol Chem 283:20864-73. 2008
    ....
  40. ncbi Sequential triage of transmembrane segments by Sec61alpha during biogenesis of a native multispanning membrane protein
    Heather Sadlish
    Department of Biochemistry and Molecular Biology, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    Nat Struct Mol Biol 12:870-8. 2005
    ..This mechanism provides a means to facilitate early folding events before release into the lipid bilayer...
  41. ncbi Identification of nascent chain interaction sites on trigger factor
    Sathish K Lakshmipathy
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Biol Chem 282:12186-93. 2007
    ..Our data indicate the existence of two regions on TF along which nascent chains can interact, the NC-domains as the main site and the PPIase domain as an auxiliary site...
  42. ncbi Docosahexaenoic acid selectively inhibits plasma membrane targeting of lipidated proteins
    Jeongmin Seo
    Department of Nutrition and Food Science, Chemistry, and Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2253, USA
    FASEB J 20:770-2. 2006
    ....
  43. pmc The interaction of the chaperonin tailless complex polypeptide 1 (TCP1) ring complex (TRiC) with ribosome-bound nascent chains examined using photo-cross-linking
    C D McCallum
    Department of Medical Biochemistry and Genetics, Texas A and M University, College Station, Texas 77843 1114, USA
    J Cell Biol 149:591-602. 2000
    ..Our results indicate that TRiC/CCT associates with the translating polypeptide shortly after it emerges from the ribosome and suggest a close association between the chaperonin and the translational apparatus...
  44. ncbi Translation arrest requires two-way communication between a nascent polypeptide and the ribosome
    Cheryl A Woolhead
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 22:587-98. 2006
    ..Based on these observations, we propose that translation arrest results from a series of reciprocal interactions between the ribosome and the C terminus of the nascent SecM polypeptide...
  45. ncbi How hydrophobic is alanine?
    Ingmarie Nilsson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    J Biol Chem 278:29389-93. 2003
    ....
  46. pmc Human Peroxin PEX3 Is Co-translationally Integrated into the ER and Exits the ER in Budding Vesicles
    Peter U Mayerhofer
    Department of Molecular and Cellular Medicine, Texas A and M Health Science Center, 440 Reynolds Medical Building, College Station, TX, 77843, USA
    Traffic 17:117-30. 2016
    ..This study identifies early steps in human peroxisomal biogenesis by demonstrating sequential stages of PMP passage through the mammalian ER. ..
  47. pmc Structural elements of the cholesterol-dependent cytolysins that are responsible for their cholesterol-sensitive membrane interactions
    Casie E Soltani
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 104:20226-31. 2007
    ..These studies provide an example of a structural motif that specifically mediates the interaction of a bacterial toxin with a cholesterol-rich membrane...
  48. ncbi Double-spanning plant viral movement protein integration into the endoplasmic reticulum membrane is signal recognition particle-dependent, translocon-mediated, and concerted
    Ana Sauri
    Departament de Bioquimica i Biologia Molecular, Universitat de València E 46 100 Burjassot, Spain
    J Biol Chem 280:25907-12. 2005
    ....
  49. ncbi Barreling through the membrane
    Arthur E Johnson
    Nat Struct Mol Biol 11:113-4. 2004
  50. ncbi Cleavage of a tail-anchored protein by signal peptidase
    Ingmarie Nilsson
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Stockholm, Sweden
    FEBS Lett 516:106-8. 2002
    ..Here, we show that a tail-anchored protein can be cleaved by signal peptidase and that the sequence requirements for efficient cleavage seem to be the same as for cleavage of co-translationally targeted SRP-dependent proteins...
  51. ncbi Exploring the capacity of trigger factor to function as a shield for ribosome bound polypeptide chains
    Sladjana Tomic
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    FEBS Lett 580:72-6. 2006
    ..Thus, TF is not a general shield for nascent chains. Protease protection appears to depend on a hydrophobic interaction of TF with nascent polypeptides...
  52. ncbi Sec61alpha and TRAM are sequentially adjacent to a nascent viral membrane protein during its ER integration
    Ana Sauri
    Departament de Bioquimica i Biologia Molecular, Universitat de València E 46 100 Burjassot, Spain
    J Mol Biol 366:366-74. 2007
    ..TRAM therefore appears to be involved in viral membrane protein integration, and nascent chain recognition by TRAM does not appear to rely solely on the TM domains...
  53. ncbi Fluorescence resonance energy transfer analysis of protein translocase. SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes
    Hiroyuki Mori
    Institute for Virus Research, Kyoto University, Japan
    J Biol Chem 278:14257-64. 2003
    ....
  54. pmc Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length
    Carolina Lundin
    Stockholm Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Svante Arrhenius vag 12, SE 106 91 Stockholm, Sweden
    FEBS Lett 581:3809-13. 2007
    ..Our results show a strong correlation between the length of an Abeta-derived segment and its ability to integrate into the microsomal membrane...
  55. ncbi Conformational changes that effect oligomerization and initiate pore formation are triggered throughout perfringolysin O upon binding to cholesterol
    Alejandro P Heuck
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 282:22629-37. 2007
    ..The interaction of PFO with cholesterol is therefore sufficient to initiate an irreversible sequence of coupled conformational changes that extend throughout the toxin molecule...
  56. pmc Specific protein-membrane contacts are required for prepore and pore assembly by a cholesterol-dependent cytolysin
    Casie E Soltani
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 282:15709-16. 2007
    ..These studies reveal that pore formation by ILY, and probably other CDCs, is affected by an intricate and coupled sequence of interactions between domain 4 and the membrane...
  57. pmc SRP keeps polypeptides translocation-competent by slowing translation to match limiting ER-targeting sites
    Asvin K K Lakkaraju
    Departement de Biologie Cellulaire, Universite de Geneve, Sciences III, 1211 Geneva, Switzerland
    Cell 133:440-51. 2008
    ..SRP therefore ensures that nascent chains remain translocation competent during the targeting time window dictated by SR. Since SRP-signal sequence affinities vary, the delay may also regulate which proteins are preferentially targeted...
  58. pmc Bcl-2 homodimerization involves two distinct binding surfaces, a topographic arrangement that provides an effective mechanism for Bcl-2 to capture activated Bax
    Zhi Zhang
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA
    J Biol Chem 279:43920-8. 2004
    ..Our data support a simple model mechanism by which Bcl-2 interacts with activated Bax during apoptosis in an effective manner to neutralize the proapoptotic activity of Bax...