Affiliation: SUNY Upstate Medical University
- Ablation of the proapoptotic genes CHOP or Ask1 does not prevent or delay loss of visual function in a P23H transgenic mouse model of retinitis pigmentosaAdeseye Adekeye
Departments of Neuroscience and Physiology, Biochemistry and Molecular Biology and Ophthalmology, Center for Vision Research, SUNY Upstate Medical University, Syracuse, New York, United States of America
PLoS ONE 9:e83871. 2014..Instead they may be involved in regulating secondary pathological responses such as inflammation that are upregulated during later stages of disease progression...
- Rhodopsin mutant P23H destabilizes rod photoreceptor disk membranesMohammad Haeri
Center for Vision Research, Department of Neuroscience, State University of New York Upstate Medical University, Syracuse, New York, United States of America
PLoS ONE 7:e30101. 2012..This may render rods susceptible to mechanical injury or compromise OS function, contributing to photoreceptor loss...
- Regulation of rhodopsin-eGFP distribution in transgenic xenopus rod outer segments by lightMohammad Haeri
Departments of Neuroscience and Physiology, Biochemistry and Molecular Biology, and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York, United States of America
PLoS ONE 8:e80059. 2013..The impact of these results on the use of chimeric proteins with rhodopsin fused to fluorescent proteins at the carboxyl terminus is discussed. ..
- An inducible expression system to measure rhodopsin transport in transgenic Xenopus rod outer segmentsXinming Zhuo
Departments of Neuroscience and Physiology, Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York, United States of America
PLoS ONE 8:e82629. 2013..Using a simple model, we find that stochastic variation in transgene expression can account for the shape of the induction response. ..
- Cooperative activation of Xenopus rhodopsin transcription by paired-like transcription factorsSarah E Reks
Departments of Neuroscience and Physiology, Ophthalmology and Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, NY 13210, USA
BMC Mol Biol 15:4. 2014..Cooperative action by these transcription factors activates rod-specific genes such as rhodopsin. However, a quantitative mechanistic explanation of transcriptional rate determinants is lacking...
- Bioinformatic identification of novel putative photoreceptor specific cis-elementsCharles G Danko
Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY, USA
BMC Bioinformatics 8:407. 2007..Here, we use motif discovery algorithms to predict transcription factor binding sites involved in regulating the differences between murine rod and cone photoreceptor populations...
- Heterologous expression of limulus rhodopsinBarry E Knox
Department of Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 278:40493-502. 2003..This may be a general property of invertebrate opsins and may underlie some of the functional differences between invertebrate and vertebrate visual pigments...
- Shedding light on conesBarry E Knox
Department of Biochemistry and Department of Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, NY 13210, USA
J Gen Physiol 127:355-8. 2006
- Light responses in rods of vitamin A-deprived XenopusEduardo Solessio
Department of Ophthalmology, SUNY Upstate Medical University, Center for Vision Research, Syracuse, New York 13210, USA
Invest Ophthalmol Vis Sci 50:4477-86. 2009..Contrasting phototransduction in vitamin A-deprived Xenopus rods with phototransduction in constitutively active mammalian rods may provide new understanding of the mechanisms that lead to retinal degeneration...
- Circadian modulation of temporal properties of the rod pathway in larval XenopusEduardo Solessio
Department of Ophthalmology and Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
J Neurophysiol 92:2672-84. 2004..The temporal properties of the behaviorally measured rod mechanism correspond closely with those of the electrophysiologically measured retinal response, indicating that the rod signals are modulated at the level of the outer retina...
- Developmental regulation of calcium-dependent feedback in Xenopus rodsEduardo Solessio
Center for Vision Research, Weiskotten Hall, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
J Gen Physiol 124:569-85. 2004..We conclude that larval Xenopus maximize sensitivity at the expense of slower response kinetics while adults maximize response kinetics at the expense of sensitivity...
- Conserved transcriptional activators of the Xenopus rhodopsin geneS Leigh Whitaker
Departments of Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
J Biol Chem 279:49010-8. 2004..Furthermore, activation of the endogenous rhodopsin gene was also observed in some animals, showing that XOtx5 and XL-Nrl can activate the promoter in native chromatin environment...
- Phototransduction by vertebrate ultraviolet visual pigments: protonation of the retinylidene Schiff base following photobleachingAbhiram Dukkipati
Departments of Chemistry and Biology, Syracuse University, 111 College Place, Syracuse, NY 13244 4100, USA
Biochemistry 41:9842-51. 2002..We conclude that the MUV-visual pigment possesses an unprotonated retinylidene Schiff base in the dark state, and undergoes a protonation event during the photobleaching cascade...
- Nr2e3 and Nrl can reprogram retinal precursors to the rod fate in Xenopus retinaVera A McIlvain
Department of Biochemistry and Molecular Biology and Ophthalmology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
Dev Dyn 236:1970-9. 2007..Misexpression of Nrl and Nr2e3 together were more effective than either transcription factor alone in directing precursors to the rod fate...
- The rod cGMP-phosphodiesterase beta-subunit promoter is a specific target for Sp4 and is not activated by other Sp proteins or CRXLeonid E Lerner
Jules Stein Eye Institute, Department of Ophthalmology, UCLA School of Medicine, Los Angeles, California 90095, USA
J Biol Chem 277:25877-83. 2002..We found that it binds purified TBP and TFIIB in gel mobility shift assays with cooperative enhancement of binding affinity...
- Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptorsJon A Peet
Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA
J Cell Sci 117:3049-59. 2004..Arrestin-EGFP mass was conserved during a large-scale, light-driven redistribution in which approximately 40% of the protein in the inner segment moved to the outer segment in less than 30 minutes...
- Regulation of photoactivation in vertebrate short wavelength visual pigments: protonation of the retinylidene Schiff base and a counterion switchLavoisier S Ramos
Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, USA
Biochemistry 46:5330-40. 2007..We examine the relevance of this observation to the counterion switch mechanism of visual pigment activation...
- Vertebrate ultraviolet visual pigments: protonation of the retinylidene Schiff base and a counterion switch during photoactivationAna Karin Kusnetzow
Departments of Chemistry and Molecular and Cell Biology, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, USA
Proc Natl Acad Sci U S A 101:941-6. 2004..The findings with MUV are in close analogy to rhodopsin and provides further support for the importance of the counterion switch in the photoactivation of both rod and cone visual pigments...
- Perspectives on the counterion switch-induced photoactivation of the G protein-coupled receptor rhodopsinRobert R Birge
Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, USA
Proc Natl Acad Sci U S A 100:9105-7. 2003
- Disruption of kinesin II function using a dominant negative-acting transgene in Xenopus laevis rods results in photoreceptor degenerationJennifer Lin-Jones
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA
Invest Ophthalmol Vis Sci 44:3614-21. 2003..In the current study, the role of kinesin II in rod photoreceptors was examined by expressing a dominant negative-acting transgene that disrupts kinesin II function in Xenopus laevis rods of transgenic tadpoles...
- Mouse cone arrestin gene characterization: promoter targets expression to cone photoreceptorsXuemei Zhu
The Mary D Allen Laboratory for Vision Research, Doheny Eye Institute, Department of Cell and Neurobiology, The Keck School of Medicine of the University of Southern California, Los Angeles, CA 90089 9112, USA
FEBS Lett 524:116-22. 2002....
- Crystal structure of cone arrestin at 2.3A: evolution of receptor specificityR Bryan Sutton
Department of Neuroscience and Cell Biology, University of Texas Medical Branch, and Sealy Center for Molecular Science and Structural Biology, Galveston, TX 77555, USA
J Mol Biol 354:1069-80. 2005..Transient arrestin binding to the photopigment in cones may be responsible for the extremely rapid regeneration and reuse of the photopigment that is essential for cone function at high levels of illumination...
- MOLECULAR MECHANISMS OF PHOTORECEPTOR FUNCTIONBarry E Knox; Fiscal Year: 2010..Once the relevant signaling pathway(s) are identified, it may be possible to therapeutically target the signaling pathways to slow or block disease progression. ..
- STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTSBarry E Knox; Fiscal Year: 2011..To accomplish this we will use an inducible heterologous over-expression system to produce milligram quantities of cone pigment. The information gained in this Aim will allow for protein crystallization trials in the future. ..