Genomes and Genes
Affiliation: State University of New York
- Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activityRaj Wadgaonkar
Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
J Cell Biochem 94:351-64. 2005..Together, these novel observations indicate that TNFalpha-induced cytoskeletal rearrangement driven by MLCK activity is necessary for TNFalpha-dependent NFkappaB activation and amplification of pro-survival signals...
- Characterization of altered patterns of endothelial progenitor cells in sickle cell disease related pulmonary arterial hypertensionFatima Anjum
SUNY Downstate Medical Center and VA Medical Center, Brooklyn, New York, USA
Pulm Circ 2:54-60. 2012..Reduced number of EPCs in SCD patients with PAH might not only give potential insight into the pathophysiological mechanisms but also might be useful for identifying suitable therapeutic targets in these patients...
- Differential regulation of sphingosine kinases 1 and 2 in lung injuryRaj Wadgaonkar
Department of Medicine, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
Am J Physiol Lung Cell Mol Physiol 296:L603-13. 2009..Collectively, our results for the first time demonstrate distinct functional roles of the two SphK isoforms in the regulation of LPS-induced lung injury...
- Thrombin induced secretion of macrophage migration inhibitory factor (MIF) and its effect on nuclear signaling in endotheliumRaj Wadgaonkar
Department of Medicine, SUNY Downstate and VA Medical Center, Brooklyn, New York 11203, USA
J Cell Biochem 105:1279-88. 2008..These results indicate that MIF secretion and ERK phosphorylation both play a necessary role in thrombin induced NF-kappaB activation...
- Regulation of c-Jun N-terminal kinase and p38 kinase pathways in endothelial cellsRaj Wadgaonkar
Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
Am J Respir Cell Mol Biol 31:423-31. 2004..Thus, these inhibitors may play an important role in limiting the inflammatory effects of TNF-alpha and IL-1beta...
- Sphingomyelin synthase 2 is one of the determinants for plasma and liver sphingomyelin levels in miceJing Liu
Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, 450 Clarkson Ave, Box 5, Brooklyn, NY 11203, USA
Arterioscler Thromb Vasc Biol 29:850-6. 2009..Sphingomyelin synthase (SMS) is the last enzyme for SM de novo biosynthesis. Two SMS genes, SMS1 and SMS2, have been cloned and characterized...
- Role of sphingomyelin synthesis in pulmonary endothelial cell cytoskeletal activation and endotoxin-induced lung injuryFatima Anjum
Department of Pulmonary and Critical Care Medicine, State University of New York Downstate Medical Center, Brooklyn, USA
Am J Respir Cell Mol Biol 47:94-103. 2012....
- Pancreatitis-associated protein 2 modulates inflammatory responses in macrophagesDomenico Viterbo
Department of Surgery, Downstate Medical Center, State University of New York, Brooklyn, NY 11203, USA
J Immunol 181:1948-58. 2008..Our results demonstrate a new function for PAP2: it stimulates macrophage activity and likely modulates the inflammatory environment of pancreatitis...
- Sphingomyelin synthase 2 (SMS2) deficiency attenuates LPS-induced lung injurySatish Gowda
SUNY Downstate and Veterans Affairs Medical Center, Brooklyn, New York, USA
Am J Physiol Lung Cell Mol Physiol 300:L430-40. 2011..Furthermore, we show that depletion of SMS2 was sufficient to decrease MAP kinase-JNK activation, severity of LPS-induced pulmonary neutrophil influx, and inflammation, suggesting a novel role of SMS2 activation in lung injury...
- A transgenic mouse with vascular endothelial over-expression of the non-muscle myosin light chain kinase-2 isoform is susceptible to inflammatory lung injury: role of sexual dimorphism and ageJaideep Moitra
Department of Medicine, University of Chicago, Chicago, IL 60637 1470, USA
Transl Res 151:141-53. 2008..These data reveal that nmMLCK2 overexpression in the endothelium exacerbates lung injury in vivo in a sexually dimorphic and age-dependent manner...
- Congenital heart block: identification of autoantibody binding site on the extracellular loop (domain I, S5-S6) of alpha(1D) L-type Ca channelEddy Karnabi
Molecular and Cellular Cardiology Program, VA New York Harbor Healthcare System and SUNY Downstate Medical Center, Brooklyn, NY 11209, USA
J Autoimmun 34:80-6. 2010..This novel finding provides insights into the potential development of therapeutic peptides that could bind to the pathogenic antibodies and prevent CHB...
- Caspase-dependent cleavage of myosin light chain kinase (MLCK) is involved in TNF-alpha-mediated bovine pulmonary endothelial cell apoptosisIrina Petrache
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
FASEB J 17:407-16. 2003..These studies convincingly demonstrate that novel interactions between the apoptotic machinery and EC MLCK exist that regulate the endothelial contractile apparatus in TNF-alpha-induced apoptosis...
- Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endotheliumRaj Wadgaonkar
Department of Medicine, Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA
J Cell Biochem 95:849-58. 2005..Together these studies suggest that the novel interaction between MIF and MLCK may have important implications for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiologic vascular events that involve MLCK...
- Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structureKrisztina Tar
Department of Medicine, Division of Biological Sciences, The University of Chicago, Chicago, Illinois, USA
J Cell Biochem 98:931-53. 2006..PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of EC cytoskeleton and barrier maintenance. Collectively, our observations clearly demonstrate the crucial role of PP2A in EC barrier protection...
- Phosphorylation of the consensus sites of protein kinase A on alpha1D L-type calcium channelOmar Ramadan
Veterans Administration, New York Harbor Healthcare System, Brooklyn, New York 11209, USA
J Biol Chem 284:5042-9. 2009..These novel findings provide new insights into the autonomic regulation of the alpha(1D) Ca(2+) channel in the heart...
- Uric Acid as a potential biomarker of pulmonary arterial hypertension in patients with sickle cell diseaseKeval Joshi
Division of Pulmonary and Critical Care Medicine, SUNY Downstate Medical Center, Brooklyn, NY 11203 USA
Indian J Hematol Blood Transfus 27:96-100. 2011..71; P < 0.0001). This correlation was independent of diuretic use. UA could be a potential marker for PAH in SCD. However, its' prognostic and pathophysiologic role in SCD patients with PAH needs to be further investigated...
- Sphingosine kinase 1 deficiency exacerbates LPS-induced neuroinflammationNatalia M Grin'kina
SUNY Downstate Medical Center, Brooklyn, New York, United States of America
PLoS ONE 7:e36475. 2012..Sphingosine kinase 1 deletion worsens the effect of the LPS. • Overexpression of SphK1 might be a potential new treatment approach to neuroinflammation...
- Sphingomyelin synthase 2 deficiency attenuates NFkappaB activationTiruneh K Hailemariam
Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
Arterioscler Thromb Vasc Biol 28:1519-26. 2008..SMS2 regulates SM levels in cell plasma membrane and lipid rafts and has a potential to regulate NFkappaB activation...
- Pulmonary vascular permeability and ischemic injury in gelsolin-deficient micePatrice M Becker
Division of Pulmonary and Critical Care Medicine, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
Am J Respir Cell Mol Biol 28:478-84. 2003..Lung wet/dry weight and gross histology following ischemia were comparable in wild-type and gsn-/- animals. These data suggest that gelsolin significantly contributes to maintenance of vascular barrier function in the lung...
- Small-interference RNA gene knockdown of pancreatitis-associated proteins in rat acute pancreatitisYin Yao Lin
SUNY Downstate Medical Center, Brooklyn, NY 11203, USA
Pancreas 36:402-10. 2008..Here, we investigated the effect of a more stable inhibition of PAP using small-interference RNA gene knockdown in vitro and in an in vivo model of experimental pancreatitis...
- Silencing of Cav1.2 gene in neonatal cardiomyocytes by lentiviral delivered shRNAEddy Karnabi
VA New York Harbor Healthcare System, New York, NY, USA
Biochem Biophys Res Commun 384:409-14. 2009..This novel approach allows for the assessments of the roles of alpha1C and alpha1D Ca channels in native myocytes and could be used to examine their roles in physiological and pathological settings...
- Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitroLaura A Martello
Department of Medicine, Division of Gastroenterology and Hepatology, Downstate Medical Center, State University of New York, Brooklyn, NY, 11203, USA
Parasitol Res 112:4177-83. 2013..These findings may provide insight into signaling pathways that mediate innate immunity to T. cruzi and pathogenesis of gastrointestinal and pancreatic alterations in Chagas disease. ..
- Macrophage migration inhibitory factor in acute lung injury: expression, biomarker, and associationsLi Gao
Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA
Transl Res 150:18-29. 2007..These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI...
- Mutation analysis of the non-muscle myosin light chain kinase (MLCK) deletion constructs on CV1 fibroblast contractile activity and proliferationRaj Wadgaonkar
Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, Maryland 21224, USA
J Cell Biochem 88:623-34. 2003..These data demonstrate the necessary role for MLCK in driving the contractile apparatus via MLC phosphorylation, which can alter fibroblast growth and contractility...