W J Chirico
Affiliation: State University of New York
- N-Ethylmaleimide inactivates a nucleotide-free Hsp70 molecular chaperoneQ Liu
Department of Anatomy and Cell Biology, State University of New York Health Science Center at Brooklyn, Brooklyn, New York 11203, USA
J Biol Chem 271:29937-44. 1996..Together the results demonstrate that Ssa1p is an NEM-sensitive factor in cytosolic extracts from yeast that stimulates post-translational translocation of proteins into organelles...
- Conformational changes of an Hsp70 molecular chaperone induced by nucleotides, polypeptides, and N-ethylmaleimideW J Chirico
Department of Anatomy and Cell Biology, State University of New York Health Science Center at Brooklyn 11203, USA
Biochemistry 37:13862-70. 1998..Furthermore, the results provide evidence for a model in which the polypeptide-binding cleft of Hsp70s is covered by an alpha-helical lid that is open in the presence of ATP, but closed in the presence of ADP...